DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Myo28B1 and Myo15

DIOPT Version :9

Sequence 1:	NP_723294.1	Gene:	Myo28B1 / 53515	FlyBaseID:	FBgn0040299	Length:	2122	Species:	Drosophila melanogaster
Sequence 2:	NP_034992.2	Gene:	Myo15 / 17910	MGIID:	1261811	Length:	3511	Species:	Mus musculus

Alignment Length:	2679	Identity:	697/2679 - (26%)
Similarity:	1056/2679 - (39%)	Gaps:	832/2679 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MDAGYSPR--KGEYVWVKPQ-NTTSEFAVPFGARIVR---------TEKTQTLVCDDRNKQFWVP 53
            :.||..||  .|.:..|.|| ...|.|. |.|...|:         .:......|..|..:.|.|
Mouse  1082 LKAGEQPRAEPGRFAVVMPQVRGVSSFR-PKGPAPVQPPEHPDQDPEQGPAPQACSLRWPRLWPP 1145

  Fly    54 ----------------------AGDVLKA-------------MH-------ITSQED-----VED 71
                                  .||..|:             ||       :.|:|.     |||
Mouse  1146 TDAHCLWSRIRTYSSQSHLRGHGGDCHKSLWKKTRPQSWQNKMHSIRNLPSMRSREQHREDGVED 1210

  Fly    72 MITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPYQILPIYTNREIQLYRNKSLAELPPHI 136
            |..|.||||.|:|.||:.|:.:.|||||.||:||::|||::..||...::|.|..::|.|.|||:
Mouse  1211 MTQLEDLQETTVLANLKTRFERNLIYTYIGSILVSVNPYRMFAIYGPEQVQQYSGRALGENPPHL 1275

  Fly   137 FAISDNAFQRLQRLKENQCVVISGESGAGKTESTKLILQYLAAISGKHSWIEQ-QIIEANPIMEA 200
            |||::.||.::...|:||||:||||||:||||:|||||:.|||::.:...::| :|:||.|::||
Mouse  1276 FAIANLAFAKMLDAKQNQCVIISGESGSGKTEATKLILRCLAAMNQRRDVMQQIKILEATPLLEA 1340

  Fly   201 FGNAKTVRNDNSSRFGKYIEIRFTPQGAIQGARIQQYLLEKSRIVFQSRDERNYHIFYCMLAGLS 265
            |||||||||||||||||::|| |...|.|.||...||||||||||||:::||||||||.:||||.
Mouse  1341 FGNAKTVRNDNSSRFGKFVEI-FLEGGVICGAITSQYLLEKSRIVFQAKNERNYHIFYELLAGLP 1404

  Fly   266 TAERERLKLQEQSPSQYHYLAQGGCFTLPGRGDAKDFADIRAAMKVLSFKPEEVWSILSLLAAIL 330
            ...|:...|||  ...|:||.|||...:.|:.||.||..:.|||:||.|..|:..||..:||:||
Mouse  1405 AQLRQAFSLQE--AETYYYLNQGGNCEIAGKSDADDFRRLLAAMEVLGFTSEDQDSIFRILASIL 1467

  Fly   331 HLGNLRF-----TATEVANLATAEIDDTPNLQRVAQLLGIPISALNAALTQRTIFVHGEHVTTSL 390
            ||||:.|     .|.|||::.:|.     .:|.||:||.:....|..|:|.:......|.:.|.|
Mouse  1468 HLGNVYFEKHETDAQEVASVVSAR-----EIQAVAELLQVSPEGLQKAITFKVTETIREKIFTPL 1527

  Fly   391 SKEAAIEGRDAFVKSLYDGIFVRIVRRINETINKQVDQPMNSIGVLDIFGFENFDNNSFEQLCIN 455
            :.|:|::.|||..|.||..:|..::.|:|..::.:  |...||.:|||:|||:...|||||||||
Mouse  1528 TVESAVDARDAIAKVLYALLFGWLITRVNALVSPK--QDTLSIAILDIYGFEDLSFNSFEQLCIN 1590

  Fly   456 YANENLQQFFVGHIFKMEQDEYQNEHINWQHIEFQDNQQILDLIGMKPMNLMSLIDEESKFPKGT 520
            |||||||..|...:|:.||:||..|.::|:.|.|.|||..::||.:||..::.::|::..||:.|
Mouse  1591 YANENLQYLFNKIVFQEEQEEYIREQMDWREIAFADNQPCINLISLKPYGILRILDDQCCFPQAT 1655

  Fly   521 DQTLLEKLHVQHGNRSIYVKGKTTQTSLFGIRHYAGVVMYNPLGFLEKNRDSFSGDLRTLVQRST 585
            |.|.|:|.|..||...:|.|.|..... |.|:||||.|.|....||:||.|....|:..|...|.
Mouse  1656 DHTFLQKCHYHHGANPLYSKPKMPLPE-FTIKHYAGKVTYQVHKFLDKNHDQVRQDVLDLFVHSR 1719

  Fly   586 NKYLVDIF--------PHEMPMDTA----KKQPTLCVKFRNSLDMLMRTLSQAHPYFIRCIKPNE 638
            .:.:..:|        |..:...::    .|..|:..||:.||..|:..:.:.:|.|:||:|||.
Mouse  1720 TRVVAHLFSSHAAQTAPPRLGKSSSITRLYKAHTVAAKFQQSLLDLVEKMERCNPLFVRCLKPNH 1784

  Fly   639 YKEPKNFDKELCVRQLRYSGMMETARIRRAGYPIRHAYRAFVERYRLL------VPPVGPLEQCD 697
            .|||..|:.::.:.||||||::||.|||:.|:|:|..::.|::|||.|      ||..|.:
Mouse  1785 KKEPGLFEPDVMMAQLRYSGVLETVRIRKEGFPVRLPFQVFIDRYRCLVALKLNVPADGDM---- 1845

  Fly   698 CRKLARQICEVALPADSDRQYGKTKLFLRDEDDASLELQRSQLMLKSIVTIQRGIRRVLFRRYMK 762
            |..|..::|.|.  .|..| .|.:||||::.....||..|.::..::.:|:||.:|....:|:.:
Mouse  1846 CVSLLSRLCTVT--PDMYR-VGISKLFLKEHLHQLLESMRERVQNRAALTLQRYLRGFFIQRHFR 1907

  Fly   763 RYREAIITVQRYWRGRLQRRKYQVMRQGFHRLGACIAAQQLTTKFTMVRCRTIKLQALSRGYLVR 827
            ..|..||.:|...||.|.|::||.|||..                       :|.::|...|:.|
Mouse  1908 SLRRKIILLQSRARGFLARQRYQQMRQSL-----------------------LKFRSLVHTYVNR 1949

  Fly   828 KDFQKKLLERRKQNQLKKEELLKLAKMKEAEELLRLQQLKEQKEREQREQQEKRLQEEQRLKAEA 892
            :.:.|...|:|::.|             ||       .|:||:|..:||....|..|   :.||.
Mouse  1950 RRYLKLRAEQRRRAQ-------------EA-------WLREQEELSKREVVPVRHLE---VPAEV 1991

  Fly   893 AARNALAMAAVQQKRRTKPVKQEAPKAPTLQARNSLPPPPTTLIVAAPLPTRPASAVTRINTIPE 957
            |   .|..||...|..:.| :....:||.|||
Mouse  1992 A---GLLQAAAGLKLSSGP-RVAVVRAPRLQA--------------------------------- 2019

  Fly   958 SPGTIDVESSKQMVDDVFRFLNDEPDAALRKLNNISSGDTIRLPKSVPNNIDTSDFSYLKYAATY 1022
                                   ||              .:.||      :|.:::...|:...:
Mouse  2020 -----------------------EP--------------CVTLP------LDINNYPMAKFIRCH 2041

  Fly  1023 FG----GGATAQHERKPLKKSLLKHEHPIDEMASK-AIWLTILRFMGDLPDVVSSPTLHVFDNEN 1082
            |.    |..|.     |||..|.:  .|::..|.. :::..|||||||       |.||
Mouse  2042 FKEPSFGMLTV-----PLKMPLTR--LPVEHHAEAISVFKLILRFMGD-------PHLH------ 2086

  Fly  1083 LMSDLASLLNTSDSYKPRLFVRQSQRRIPKPLASGEKEAQEFYQHWLNVPTSHLEKIHFIIGHGI 1147
                                                             .|..:...::|:..|:
Mouse  2087 -------------------------------------------------GTQEMILGNYIVHQGL 2102

  Fly  1148 IKNSLRDEILAQICKQLYLNPSRSSYSRGWLLLSLCLSCFPPSKEFEPHLRSFMKQ-----GTAQ 1207
            ::.:||||||||:..|::.||:..:..||||||:.|||.|.||    |||..|:.:     |...
Mouse  2103 VEPALRDEILAQLANQVWRNPNAYNSKRGWLLLAACLSGFAPS----PHLDKFLLKFVSDYGQNG 2163

  Fly  1208 LQATPSLQRLERTLVNGPRCQPPSLFELHAIRGRHPLRLDIHLMDGQQRRLQVDAASTAREAVNQ 1272
            .||....:.|:.......|..||:..|..||:.:..:.||:...:|.|....|...||.......
Mouse  2164 FQAVCQHRLLQAMGSGAARTFPPTQLEWTAIQEKASMALDVSCFNGDQFSCPVHTWSTGEAVAGD 2228

  Fly  1273 LCQGMGLTDTFGFGLVMSLNGKLMPLGAGQEHVLDAISECEQRQLDAP-WKLYI----------- 1325
            :.:..||.|.:....|...||......||.::|||.:|:.|..: |.| .|.|.
Mouse  2229 ILKHRGLADGWRGWTVAMKNGVQWAELAGHDYVLDLVSDLELLR-DFPRQKSYFIVGAEGPLAGR 2292

  Fly  1326 --RKEMFATWYDPSMD----PKATQLIYK------------------------------------ 1348
              .:.:|...:|...|    |:....:.|
Mouse  2293 GDTRGVFGNCWDSDEDTPTRPQPQDHVAKMPDLDGYCSHKEDGTNGETEAQRWTSNRQAVDSIGE 2357

  Fly  1349 -----QILNG---------LKCGE----------YRCRS-------------------------- 1363
                 :.|:|         |.||:          ||.:.
Mouse  2358 STVPPRELDGYLDSLFDPVLACGDADLEKPTAIAYRMKGGGQPGGGGGSTSEDTSRRPPEPKLKP 2422

  Fly  1364 ------------------------------------------------EKDIAMVCALACFVEYG 1380
                                                            |:.:|........|..|
Mouse  2423 IPGLDASTLALQQAFIHRQAVLLAREMTLQALALQQQPLSATSRPQLPERPLAPEARPKTVVGTG 2487

  Fly  1381 P---------------------------------------------------------------- 1381
            |
Mouse  2488 PPAKPVLVRPTPQSWAPGSVAKAPKIPSKPVAVPILAQDWTAPESISASPELVRYSTLNSEHFPQ 2552

  Fly  1382 ----------------------------GEILRLKPS--------------------------EI 1392
                                        |::.|..|.                          |.
Mouse  2553 PTQQIRSIIKQYKQPPWAGHPEARRTDGGKVFRRPPDPHEEALMILKGQKTQLAVVPGTQVSREA 2617

  Fly  1393 TAFV-----------------PSDLLAPGERAIEN-----------------WSRLI--AATYEK 1421
            .|.|                 ||..|.|.|..::.                 |...:  ...|.|
Mouse  2618 VAMVKPVTSAPRPCMGPTPVQPSRSLEPPEDPVQTQLHRLVNPNFYGYQDIPWRIFLRKEVFYPK 2682

  Fly  1422 SSYVKEEQNDLLL---------EAQKRAKED-----ICLFAHL---------------------- 1450
            .:|....|.|||.         ||..|..||     ..|||..
Mouse  2683 DNYSHPVQLDLLFRQILHDTFSEACLRISEDERLQMKALFAQNQLDTQRPLVTESVKRAAISMAR 2747

  Fly  1451 -SWPMRHSRLFEVVRKEGPKLQSDELMLGINSAGLFLIDETE---------QVLASCCFSEVLKV 1505
             ||.:..||||..:...|..:|    :|.::..|:.|:...:         :||.:..|:::|.|
Mouse  2748 DSWEIYFSRLFPAMGSVGTGVQ----ILAVSHTGIKLLQMVKGSKEASRRLRVLCAYSFADILFV 2808

  Fly  1506 HVESDDKLHVMTFQHVNFVLQCSSAQDANEVINYMLDNLRQRSSYGVALDPVVEGDLEDCLVLNP 1570
            .:.|.:.|. ....:...:|..:.||....:::..:..|::.|.|.||:...:..|.| .|..:.
Mouse  2809 TMPSQNMLE-FNLSNEKLILFSARAQQVKTLVDTFILELKKDSDYVVAVRNFLSEDPE-LLSFHK 2871

  Fly  1571 GDLIE----------FEAG-------VTGAQLMAGNAQDCYR-GCVNGQWGQFLAGNVRVLATLT 1617
            ||:|.          :.||       |...:|........:| |.|:|:.|:|
Mouse  2872 GDIIHLQSLEPTRVGYSAGCVVRKKLVYLEELRRRGPDFGWRFGAVHGRVGRF------------ 2924

  Fly  1618 KPSEKLQDILREGRFQEPPKP---------------TPRANYSRRRQ------------------ 1649
             |||.:|........|.|.:|               .......|||:
Mouse  2925 -PSELVQPAAAPDFLQLPAEPGRGRAAAVAAAVASAAAAQEVGRRREGPPVRARSADSGEDSIAL 2988

  Fly  1650 --HNISQLAESHFREPLDSDKAPLSKFSPEPLKAPLLKAVV---KVPPLFQQALV---------- 1699
              ..:.:.|:.:||:|....:..|...|.|..::..|:.|:   |||  .|::|:
Mouse  2989 PPSTMLEFAQKYFRDPRRRPRDGLKLKSKEDRESKTLEDVLCFTKVP--IQESLIELSDSNLNKM 3051

  Fly  1700 ---MHHHILKYMGDI---ARSNLPVNTDLIFQPALQHPLLCDELYCQLMKQLSDN--PSSESEKR 1756
               |...::::|||.   .:|.|.|...|: :....|.::.||.|||::||::||  |..:|.:|
Mouse  3052 AVDMFVAVMRFMGDAPLKGQSELDVLCTLL-KLCGDHEVMRDECYCQIVKQITDNSSPKQDSCQR 3115

  Fly  1757 GWDLLYLATGLVAPSVLVMRELIILLR-------MRADALADACLKRLKRSLAQGQRKKAPHLIE 1814
            ||.|||:.....:.|.:....||..|:       :....:|.||.:.|:::|..|.|.:.|..:|
Mouse  3116 GWRLLYIMAAYYSCSEVFYPYLIRFLQHVSWTPGLPFQGIAKACEQNLQKTLRFGGRLEFPSNME 3180

  Fly  1815 VEGI-----QQRCLHIYHKIYFPDDTVEAFEIESHTRGAELIADIAQRLELKSPVG---YSIFLK 1871
            :..:     .:|.|.:     .|.......:|::.|...::|..:...:.|..|..   |.||:.
Mouse  3181 LRAMLAGRSSKRQLFL-----LPGGLERHLKIKTCTVALDVIEGLCTEMALTRPEAFDEYVIFVV 3240

  Fly  1872 T--GDRVYAMPEEEFVFDFITQLIYWLRQQRTIRSISDGQYQLHFMRKLWLNNHPGEDLNGDMI- 1933
            |  |..|..:....::.|..:::     :|      .||.|.|.|.|.||  :.|.:..|...:
Mouse  3241 TNRGQHVCPLSCRAYILDVASEM-----EQ------VDGGYTLWFRRVLW--DQPLKFENELYVT 3292

  Fly  1934 FSYPQELHKYLKGYY-------PID--CEQASRLAILVYSA-DHDVSLQRLPEV--LTRLIPEDL 1986
            ..|.|.|..||||.:       |.:  .:|.|:||.|.:.| ||..    ||.|  :...||..|
Mouse  3293 MHYNQVLPDYLKGLFSSVPARQPTEQQLQQVSKLASLQHRAKDHFY----LPSVREVQEYIPAQL 3353

  Fly  1987 IP-------LQTVAEWRQQILPKVHRDHLTEDHAKILFLQELSHFACFGSTFFVVKQQNDDALPE 2044
            ..       |..|::.|||      ...|:...|:..||..||.|..|||:||.::..::..:|.
Mouse  3354 YHTTAGDTWLNLVSQHRQQ------TQALSPHQARAQFLGLLSAFPLFGSSFFFIQSCSNVLVPA 3412

  Fly  2045 TLLIAINSTGFHMLDPTTKEILRSYEYSQL-GIW------SSGKNHFHIRFGNMIGASKLLCSTT 2102
            ..::|:|..|.:.|...|.|::......:: ..|      :|...:..|..|::.....:.....
Mouse  3413 PCILAVNHNGLNFLSTKTHELIVKIPLKEIQSTWTQQPTANSSYPYVEISLGDVAAQRTMQLQLE 3477

  Fly  2103 QGYKMDDLLASYVK 2116
            ||.::..::|.:|:
Mouse  3478 QGLELCRVVAVHVE 3491

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Myo28B1	NP_723294.1	MYSc	62..737	CDD:214580	308/710 (43%)
		MYSc_Myo7	81..726	CDD:276832	293/668 (44%)
		IQ	765..785	CDD:197470	8/19 (42%)
		DUF4355	827..>887	CDD:290964	15/59 (25%)
		MyTH4	1031..1239	CDD:295320	55/213 (26%)
		B41	1248..1429	CDD:214604	54/486 (11%)
		FERM_N	1248..>1288	CDD:286467	8/39 (21%)
		FERM_B-lobe	1342..1429	CDD:271216	28/374 (7%)
		PH-like	1454..1547	CDD:302622	20/101 (20%)
		MyTH4	1674..1819	CDD:214535	48/177 (27%)
		B41	1827..2032	CDD:214604	61/229 (27%)
		FERM_N	1829..>1877	CDD:286467	11/52 (21%)
		FERM_M	1936..2032	CDD:278785	37/114 (32%)
		FERM_C2_MyoVII	2028..2121	CDD:270020	19/96 (20%)
Myo15	NP_034992.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..44
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	574..690
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	712..1030
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1105..1135		5/30 (17%)
		MYSc	1201..1883	CDD:214580	306/699 (44%)
		MYSc_Myo15	1220..1871	CDD:276838	293/668 (44%)
		Actin-binding. /evidence=ECO:0000255	1776..1783		4/6 (67%)
		FliJ	1870..1960	CDD:280262	28/112 (25%)
		Neck or regulatory domain	1872..2013		47/190 (25%)
		Tail	2014..3511		323/1678 (19%)
		MyTH4	2049..2195	CDD:214535	56/218 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2330..2359		0/28 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2392..2425		2/32 (6%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2460..2509		5/48 (10%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2565..2584		1/18 (6%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2629..2648		4/18 (22%)
		SH3	2852..2931	CDD:302595	22/92 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2964..2984		3/19 (16%)
		MyTH4	3031..3185	CDD:214535	44/156 (28%)
		B41	3195..3400	CDD:214604	61/232 (26%)
		FERM_B-lobe	3290..3390	CDD:271216	31/109 (28%)
		FERM_C_MyoXV	3396..3497	CDD:270022	19/96 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG4229
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100101
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.710