DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Spt5 and SPT5

DIOPT Version :9

Sequence 1:	NP_652610.1	Gene:	Spt5 / 53442	FlyBaseID:	FBgn0040273	Length:	1078	Species:	Drosophila melanogaster
Sequence 2:	NP_013703.1	Gene:	SPT5 / 854999	SGDID:	S000004470	Length:	1063	Species:	Saccharomyces cerevisiae

Alignment Length:	924	Identity:	256/924 - (27%)
Similarity:	430/924 - (46%)	Gaps:	148/924 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 DSEVSNMSDSGSEDGSISNKSQRSARSKSRSRSRSGSRGSRSVSRSRSRSQSGHSRSGSESPQRR 67
            ::|.:......|.:|..::.:|.:..:.|...:....:...|..|.|.|.:           ..:
Yeast    81 NAETAATEQVSSSNGPATDDAQATLNTDSSEANEIVKKEEGSDERKRPREE-----------DTK 134

  Fly    68 DNRGKSDESGEEEEEPPGEDIDSEEYDEEENDDHPRKKKKKERFGGFIIDEAEVDDEVDEDDEWE 132
            ::.|.:.:.|:.::|...||.|.::.||:::|:.|.|::::|| ..|:..||||.|:.|||::.|
Yeast   135 NSDGDTKDEGDNKDEDDDEDDDDDDDDEDDDDEAPTKRRRQER-NRFLDIEAEVSDDEDEDEDEE 198

  Fly   133 EGANEI---GIVGNEIDE----LGPTA-RDIEIRRR-GTNLWDTQKED--EIEEYLRKKYADESI 186
            :  :|:   |.:.:..||    ..|.| ||..:.|: ..:|..|.:||  .:.:.||::|. .|.
Yeast   199 D--SELVREGFITHGDDEDDEASAPGARRDDRLHRQLDQDLNKTSEEDAQRLAKELRERYG-RSS 260

  Fly   187 AKRHFG---DGGEEMSDEITQQTLLPGIKDPNLWMVKCRIGEEKATALLLMRKYLTYLNTD---- 244
            :|::..   ||      .:.|:.|||.:....:|.|:||.|:||.....|::|   ..|.|
Yeast   261 SKQYRAAAQDG------YVPQRFLLPSVDTATIWGVRCRPGKEKELIRKLLKK---KFNLDRAMG 316

  Fly   245 -DPLQIKSIIAPEGVKGYIYLEAYKQTHVKTCIDNVGNLRMGKWKQEMVPIKEMTDVLKVVK-EQ 307
             ..|:|.||...:...|.||:||.||:.::...:.|.::.:.  ::.::|::|:..:||..| :.
Yeast   317 KKKLKILSIFQRDNYTGRIYIEAPKQSVIEKFCNGVPDIYIS--QKLLIPVQELPLLLKPNKSDD 379

  Fly   308 VGLKVKQWVRLKRGLYKDDIAQVDYVDLAQNQVHLKLLPRIDYTRMRGALRTTATESDDSKRKKK 372
            |.|:...:||:|||:||.|:|.||.:.....:|.||::||:||.:......||       :::|.
Yeast   380 VALEEGSYVRIKRGIYKGDLAMVDQISENNLEVMLKIVPRLDYGKFDEIDPTT-------QQRKS 437

  Fly   373 RRPA----AKP--FDPE-AVRAIGGEVHSDGDFLLFEGNRYSR-------------KGFLYKNFT 417
            |||.    |.|  |:|. |:|             |.:.|.|.|             .|:|||:|.
Yeast   438 RRPTFAHRAPPQLFNPTMALR-------------LDQANLYKRDDRHFTYKNEDYIDGYLYKSFR 489

  Fly   418 MSAILSDGVKPTLAELERFEESPEEVNL-EIMGTVKDDPTMAHSFSMGDNVEVCVGDLENLQAKI 481
            :..:.:..::||:.||.||......|:| .:..::|.......:|..||.:||..|:....:..:
Yeast   490 IQHVETKNIQPTVEELARFGSKEGAVDLTSVSQSIKKAQAAKVTFQPGDRIEVLNGEQRGSKGIV 554

  Fly   482 VAIDGTMITVMPKHQDLKDPLIFKASELRKYFKTGDHARVLAGRYEGETGLIIRVEPTRVVLVSD 546
            ......:.|:  |......||.|..|.|||.|:.|||..|:.|.::|:.||::.||..:|..:|.
Yeast   555 TRTTKDIATI--KLNGFTTPLEFPISTLRKIFEPGDHVTVINGEHQGDAGLVLMVEQGQVTFMST 617

  Fly   547 LTNHELEVLPRDLQLCSD-VATGVDCLGQFQWGDMVQLDSQNVGVIVRLERENFHVLGMNGKCIE 610
            .|:.|:.:...:|....| .||.    .::...|:|:|.::||..|::...:.|.|:...||...
Yeast   618 QTSREVTITANNLSKSIDTTATS----SEYALHDIVELSAKNVACIIQAGHDIFKVIDETGKVST 678

  Fly   611 -CKPTALHKRKENRHTV-ALDADQNQIRRRDVVKVMEGPHAGRSGEIKHLYRSLAFLHCRMYTEN 673
             .|.:.|.|....|..| ::||:.|:|:..|.  ::|...:.|.|::.::.....|:..:...||
Yeast   679 ITKGSILSKINTARARVSSVDANGNEIKIGDT--IVEKVGSRREGQVLYIQTQQIFVVSKKIVEN 741

  Fly   674 GGIFVCKTRHLQLAGGSKTTVSNAGIVGGLGFMSPRIQSPMHPSGGRGARGGARGGRGGFRVTRD 738
            .|:||....:::........:||.   ..|..|:|.|.|.|.|...:..:...: .|||
Yeast   742 AGVFVVNPSNVEAVASKDNMLSNK---MDLSKMNPEIISKMGPPSSKTFQQPIQ-SRGG------ 796

  Fly   739 REI-LGKTIKISGGPYKGAVGIVKDATESTARVELHTSCQTISVDRNHIAIVGVTGKEG-----S 797
            ||: ||||::|....|||.:|||||.....|.||||:..:.|::|::.:......|.||     .
Yeast   797 REVALGKTVRIRSAGYKGQLGIVKDVNGDKATVELHSKNKHITIDKHKLTYYNREGGEGITYDEL 861

  Fly   798 VSTYGRTPARTPGYGAQTPSYT--------------AAGSKTPLVGSQTPNWDTDTRTPYGTMTP 848
            |:..||.|....|     |||.              ||.:.:.|.|..||.|.
Yeast   862 VNRRGRVPQARMG-----PSYVSAPRNMATGGIAAGAAATSSGLSGGMTPGWS------------ 909

  Fly   849 SHDGSMTP---RHG 859
            |.||..||   .||
Yeast   910 SFDGGKTPAVNAHG 923

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Spt5	NP_652610.1	Spt5_N	<136..208	CDD:288770	21/85 (25%)
		nusG	212..352	CDD:181467	48/145 (33%)
		NGN_Euk	215..302	CDD:193577	25/91 (27%)
		KOW_Spt5_1	313..350	CDD:240505	16/36 (44%)
		KOW_Spt5_2	461..511	CDD:240506	13/49 (27%)
		KOW_Spt5_3	512..562	CDD:240507	16/49 (33%)
		KOW_Spt5_4	638..680	CDD:240508	10/41 (24%)
		KOW_Spt5_5	737..788	CDD:240509	22/51 (43%)
		CTD	812..908	CDD:215026	17/65 (26%)
		CTD	812..870	CDD:289577	17/65 (26%)
		KOW_Spt5_6	1020..1075	CDD:240510
SPT5	NP_013703.1	PRK08581	2..>142	CDD:236304	10/71 (14%)
		Spt5_N	180..280	CDD:403226	32/108 (30%)
		NGN_Euk	287..373	CDD:193577	25/90 (28%)
		KOW_Spt5_1	385..422	CDD:240505	16/36 (44%)
		SPT5	441..1059	CDD:227493	146/531 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157344167
Domainoid	1	1.000	91	1.000	Domainoid score	I1756
eggNOG	1	0.900	-	-		E1_COG5164
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2384
Inparanoid	1	1.050	279	1.000	Inparanoid score	I566
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54675
OrthoFinder	1	1.000	-	-		FOG0003457
OrthoInspector	1	1.000	-	-		oto99025
orthoMCL	1	0.900	-	-		OOG6_101219
Panther	1	1.100	-	-	LDO	PTHR11125
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1387
SonicParanoid	1	1.000	-	-		X2825
TreeFam	1	0.960	-	-
	15	14.790