DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Spt5 and SUPT5H

DIOPT Version :9

Sequence 1:	NP_652610.1	Gene:	Spt5 / 53442	FlyBaseID:	FBgn0040273	Length:	1078	Species:	Drosophila melanogaster
Sequence 2:	NP_001104490.1	Gene:	SUPT5H / 6829	HGNCID:	11469	Length:	1087	Species:	Homo sapiens

Alignment Length:	1136	Identity:	596/1136 - (52%)
Similarity:	771/1136 - (67%)	Gaps:	114/1136 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSDSEVSNMSDSGSEDGSISNKSQRSARSKSRSRSRSGSRGSRSVSRSRSRSQSGHSRSGSESPQ 65
            |||||.||.|:  .||...|:..:.:...:.|                       .|.:|||
Human     1 MSDSEDSNFSE--EEDSERSSDGEEAEVDEER-----------------------RSAAGSE--- 37

  Fly    66 RRDNRGKSDESGEEEEEPPGEDIDSEEYDEEENDDHPRKKKKKERFGGFIIDEAEVDDEVDEDDE 130
                  |.:|..:||||...|:.|.||  |||:||.|   .||.|.||||:|||:||||.:::|:
Human    38 ------KEEEPEDEEEEEEEEEYDEEE--EEEDDDRP---PKKPRHGGFILDEADVDDEYEDEDQ 91

  Fly   131 WEEGANEI----GIVGNEIDELGPTARDIEIRRRGTNLWDTQKEDEIEEYLRKKYADESIAKRHF 191
            ||:||.:|    .|..:.||.: ....|....||..|||..|:|:|:.||..||||..|:.:..:
Human    92 WEDGAEDILEKEEIEASNIDNV-VLDEDRSGARRLQNLWRDQREEELGEYYMKKYAKSSVGETVY 155

  Fly   192 GDGGEEMSDEITQQTLLPGIKDPNLWMVKCRIGEEKATALLLMRKYLTYLNTDDPLQIKSIIAPE 256
            | |.:|:||:||||.||||:||||||.|||:||||:|||:.||||::.|..||.||||||::|||
Human   156 G-GSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPE 219

  Fly   257 GVKGYIYLEAYKQTHVKTCIDNVGNLRMGKWKQEMVPIKEMTDVLKVVKEQVGLKVKQWVRLKRG 321
            .||||||:|||||||||..|:.|||||:|.|.|:||||||||||||||||...||.|.|||||||
Human   220 HVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVRLKRG 284

  Fly   322 LYKDDIAQVDYVDLAQNQVHLKLLPRIDYTRMRGALRTTATESDDSKRKKKRRPAAKPFDPEAVR 386
            :|||||||||||:.:||.:.||::|||||.|::..:   :.:...:||||.:||..:.||.|.:|
Human   285 IYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARM---SLKDWFAKRKKFKRPPQRLFDAEKIR 346

  Fly   387 AIGGEVHSDGDFLLFEGNRYSRKGFLYKNFTMSAILSDGVKPTLAELERFEESPEEVNLEIMGTV 451
            ::||:|.||||||:||||||||||||:|:|.|||::::||||||:|||:||:.||.::||:: |.
Human   347 SLGGDVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVV-TE 410

  Fly   452 KDDPTMAHSFSMGDNVEVCVGDLENLQAKIVAIDGTMITVMPKHQDLKDPLIFKASELRKYFKTG 516
            .......|:|..|||||||.|:|.|||.||:::||..||:||||:||||.|.|.|.|||||||.|
Human   411 STGKEREHNFQPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMG 475

  Fly   517 DHARVLAGRYEGETGLIIRVEPTRVVLVSDLTNHELEVLPRDLQLCSDVATGVDCLGQFQWGDMV 581
            ||.:|:|||:||:||||:|||...|:|.||||.|||:||||||||||:.|:|||..||.:||::|
Human   476 DHVKVIAGRFEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELV 540

  Fly   582 QLDSQNVGVIVRLERENFHVLGMNGKCIECKPTALHKRKENRHTVALDADQNQIRRRDVVKVMEG 646
            |||.|.||||||||||.|.||.|.||.:..:..|:.::|:||..||||::||.|..:|:|||::|
Human   541 QLDPQTVGVIVRLERETFQVLNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIVKVIDG 605

  Fly   647 PHAGRSGEIKHLYRSLAFLHCRMYTENGGIFVCKTRHLQLAGGSKTTVSNAGIVGGLGFMSPRIQ 711
            ||:||.|||:||:||.|||||:...||||:||||||||.||||||........|||...|||||.
Human   606 PHSGREGEIRHLFRSFAFLHCKKLVENGGMFVCKTRHLVLAGGSKPRDVTNFTVGGFAPMSPRIS 670

  Fly   712 SPMHPS-----GGRGARGGARGGRGGFRVTRDREILGKTIKISGGPYKGAVGIVKDATESTARVE 771
            ||||||     ||.|:.||..||....|..||.|::|:|::||.|||||.:|:||||||||||||
Human   671 SPMHPSAGGQRGGFGSPGGGSGGMSRGRGRRDNELIGQTVRISQGPYKGYIGVVKDATESTARVE 735

  Fly   772 LHTSCQTISVDRNHIAIVGVTGKEGSVSTYGRTPARTPGYGAQTPSYTAAGSKTPLVGSQTPNWD 836
            ||::||||||||..:..||.....|..|||||||.    ||:|||.| .:||:||:.|||||..|
Human   736 LHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRTPM----YGSQTPMY-GSGSRTPMYGSQTPLQD 795

  Fly   837 TDTRTP-YGTMTPSHDGSMTP-RHGAWDPTANTTPAR-NNDFDYSL-EEPSPSP-GY----NPST 892
             .:||| ||:.||.||||.|| :.|||||....||:| ..:::|:. :||:||| .|    ||.|
Human   796 -GSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQT 859

  Fly   893 PGY------QMTSQFAPQTPGT--LYGSDRSYSP---------FNPSPSPAP----SPYPVGYMN 936
            |||      |:..|:.||||||  :|.:|: :||         :.|||||..    :|.|.||.|
Human   860 PGYPDPSSPQVNPQYNPQTPGTPAMYNTDQ-FSPYAAPSPQGSYQPSPSPQSYHQVAPSPAGYQN 923

  Fly   937 T--------------------PSPSTYSPNTPGGIPQSPYNPQTPGASLDSSMGDWCTTDIEVRI 981
            |                    |||..|||.|||......|||.|||:.::.:..||.||||:|::
Human   924 THSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVKV 988

  Fly   982 H-THDDTDLVGQTGIIRTVSNGVCSVFLRQEDRSVSIVSEHLAPVLPCNGDEFKIIYGDDRESVG 1045
            . |:.||.:|||||:||:|:.|:|||:|:..::.|||.||||.|:.|...::.|:|.|:|||:.|
Human   989 RDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATG 1053

  Fly  1046 RVLSKDGDVFVCR--INEEIKLLPINFLCKM 1074
            .:||.||:..:.|  ::|::|:|.:.||.|:
Human  1054 VLLSIDGEDGIVRMDLDEQLKILNLRFLGKL 1084

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Spt5	NP_652610.1	Spt5_N	<136..208	CDD:288770	29/75 (39%)
		nusG	212..352	CDD:181467	102/139 (73%)
		NGN_Euk	215..302	CDD:193577	63/86 (73%)
		KOW_Spt5_1	313..350	CDD:240505	26/36 (72%)
		KOW_Spt5_2	461..511	CDD:240506	32/49 (65%)
		KOW_Spt5_3	512..562	CDD:240507	35/49 (71%)
		KOW_Spt5_4	638..680	CDD:240508	27/41 (66%)
		KOW_Spt5_5	737..788	CDD:240509	35/50 (70%)
		CTD	812..908	CDD:215026	55/110 (50%)
		CTD	812..870	CDD:289577	34/59 (58%)
		KOW_Spt5_6	1020..1075	CDD:240510	23/57 (40%)
SUPT5H	NP_001104490.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..92		47/129 (36%)
		Spt5_N	<93..172	CDD:403226	33/80 (41%)
		Interaction with SUPT4H1	176..270		70/93 (75%)
		NGN_Euk	178..265	CDD:193577	63/86 (73%)
		KOW_Spt5_1	276..313	CDD:240505	26/36 (72%)
		Interaction with RNA polymerase II	313..420		55/110 (50%)
		SPT5	334..965	CDD:227493	350/638 (55%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	670..700		14/29 (48%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	747..978		99/237 (42%)
		9 X 7 AA approximate tandem repeats of G-S-[QR]-T-P-X-[YQ], motif CTR1	754..817		38/68 (56%)
		10 X 8 AA approximate tandem repeats of P-[TS]-P-S-P-[QA]-[SG]-Y, motif CTR2	844..950		37/106 (35%)
		KOW_Spt5_6	1028..1084	CDD:240510	22/55 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165148114
Domainoid	1	1.000	547	1.000	Domainoid score	I256
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2384
Inparanoid	1	1.050	1103	1.000	Inparanoid score	I232
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54675
OrthoDB	1	1.010	-	-		D166902at33208
OrthoFinder	1	1.000	-	-		FOG0003457
OrthoInspector	1	1.000	-	-		oto88513
orthoMCL	1	0.900	-	-		OOG6_101219
Panther	1	1.100	-	-	LDO	PTHR11125
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1387
SonicParanoid	1	1.000	-	-		X2825
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	15	14.900