DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Spt5 and supt5h

DIOPT Version :9

Sequence 1:	NP_652610.1	Gene:	Spt5 / 53442	FlyBaseID:	FBgn0040273	Length:	1078	Species:	Drosophila melanogaster
Sequence 2:	XP_005157586.1	Gene:	supt5h / 64275	ZFINID:	ZDB-GENE-001207-1	Length:	1086	Species:	Danio rerio

Alignment Length:	1146	Identity:	599/1146 - (52%)
Similarity:	769/1146 - (67%)	Gaps:	132/1146 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSDSEVSNMSDSGSEDGSISNKSQRSARSKSRSRSRSGSRGSRSVSRSRSRSQSGHSRSGSESPQ 65
            |||||.|:.||         |:|:||:.::             .|..:....:.| |.:||:
Zfish     1 MSDSEDSDFSD---------NQSERSSEAE-------------EVEENEEEEEQG-SVAGSD--- 39

  Fly    66 RRDNRGKSDESGEEEEEPPGEDIDSEEYD--EEENDDHPRKKKKKERFGGFIIDEAEVDDEVDED 128
                  |::|.||:.|       |.||||  |||:||.||   ||.|.||||:|||:||||.:::
Zfish    40 ------KAEEEGEDLE-------DEEEYDEEEEEDDDRPR---KKARHGGFILDEADVDDEYEDE 88

  Fly   129 DEWEEGANEIGIVGNE-----------IDELGPTARDIEIRRRGTNLWDTQKEDEIEEYLRKKYA 182
            |.||:||.:|....||           :||      |....||..|||...:|:.:.||..:|||
Zfish    89 DPWEDGAEDILEKVNEEAEVSNLDHVVLDE------DHSGSRRLQNLWRDSREEALGEYYMRKYA 147

  Fly   183 DESIAKRHFGDGGEEMSDEITQQTLLPGIKDPNLWMVKCRIGEEKATALLLMRKYLTYLNTDDPL 247
             :|....||..|.|::||:||||.||||:||||||.|||:||||:|||:.||||::.|..||.||
Zfish   148 -KSSGGEHFYGGSEDLSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFVAYQCTDTPL 211

  Fly   248 QIKSIIAPEGVKGYIYLEAYKQTHVKTCIDNVGNLRMGKWKQEMVPIKEMTDVLKVVKEQVGLKV 312
            ||||::|||.||||||:|||||||||..|:.|||||||.|.|:||||||||||||||||...||.
Zfish   212 QIKSVVAPEHVKGYIYVEAYKQTHVKAAIEGVGNLRMGFWNQQMVPIKEMTDVLKVVKEVTNLKP 276

  Fly   313 KQWVRLKRGLYKDDIAQVDYVDLAQNQVHLKLLPRIDYTRMRGALRTTATESDDSKRKKKRRPAA 377
            |.|||||||||||||||||||:.:||.:.||::||||..|::..:   :.:...:||||.:||..
Zfish   277 KSWVRLKRGLYKDDIAQVDYVEPSQNTISLKMIPRIDLDRIKARM---SMKDWFAKRKKFKRPPQ 338

  Fly   378 KPFDPEAVRAIGGEVHSDGDFLLFEGNRYSRKGFLYKNFTMSAILSDGVKPTLAELERFEESPEE 442
            :.||.|.:|::||||..||||::||.|||||||||:|:|.|||::::||||||:|||:||:.||.
Zfish   339 RLFDAEKIRSLGGEVSHDGDFMIFEANRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEG 403

  Fly   443 VNLEIMGTVKDDPTMAHSFSMGDNVEVCVGDLENLQAKIVAIDGTMITVMPKHQDLKDPLIFKAS 507
            ::||:: |........|:...|||||||.|:|.|||.||:::||..||:||||:||||||.|.|.
Zfish   404 IDLEVV-TETTGKEREHNLQAGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDPLEFPAH 467

  Fly   508 ELRKYFKTGDHARVLAGRYEGETGLIIRVEPTRVVLVSDLTNHELEVLPRDLQLCSDVATGVDCL 572
            ||||||:.|||.:|:||||||:||||:|||...|:|.||||.|||:||||||||||:.|:|||..
Zfish   468 ELRKYFRMGDHVKVIAGRYEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDAG 532

  Fly   573 GQFQWGDMVQLDSQNVGVIVRLERENFHVLGMNGKCIECKPTALHKRKENRHTVALDADQNQIRR 637
            ||.:||::||||.|.||||||||||.|.||.|:||.:..:..|:::||:||..||||::||.|..
Zfish   533 GQHEWGELVQLDPQTVGVIVRLERETFQVLNMHGKVLTVRHQAVNRRKDNRFAVALDSEQNNIHV 597

  Fly   638 RDVVKVMEGPHAGRSGEIKHLYRSLAFLHCRMYTENGGIFVCKTRHLQLAGGSKTTVSNAGIVGG 702
            :|:|||::|||:||.|||:|::|..|||||:...||||:||||.|||.||||||........|||
Zfish   598 KDIVKVIDGPHSGREGEIRHIFRGFAFLHCKKLVENGGMFVCKARHLVLAGGSKPRDVTNFTVGG 662

  Fly   703 LGFMSPRIQSPMHPSGG----RGARGGARGGRGGFRVTRDREILGKTIKISGGPYKGAVGIVKDA 763
            ...|||||.|||||.||    ||..||..||.|..|..||.:::|:|::||.|||||.:|:||||
Zfish   663 FAPMSPRISSPMHPGGGGQPQRGGGGGGGGGMGRGRGRRDNDLIGQTVRISQGPYKGYIGVVKDA 727

  Fly   764 TESTARVELHTSCQTISVDRNHIAIVGVTGKEGSVSTYGRTPARTPGYGAQTPSYTAAGSKTPLV 828
            ||||||||||::||||||||..:..||...::|..||:    .|||.||:|||.| ..||:||:.
Zfish   728 TESTARVELHSTCQTISVDRQRLTTVGGKERQGRSSTH----LRTPMYGSQTPIY-GTGSRTPMY 787

  Fly   829 GSQTPNWDTDTRTP-YGTMTPSHDGSMTP-RHGAWDPTANTTPARNND---FDYSLEEPSPSP-G 887
            |||||..| .:||| ||:.||.||||.|| :.|||||....||:|.:|   |.|. :|||||| |
Zfish   788 GSQTPLHD-GSRTPHYGSQTPLHDGSRTPGQSGAWDPNNPNTPSRPDDEYEFAYD-DEPSPSPQG 850

  Fly   888 Y----NPSTPGY------QMTSQFAPQTPGT--LYGSDRSYSP---------FNPSPSPAP---- 927
            |    ||.||||      |:..|:.||||||  :|.::: |||         :.|||||..
Zfish   851 YGGTPNPQTPGYPEVPSPQVNPQYNPQTPGTPAMYNTEQ-YSPYAAPSPQGSYQPSPSPQSYHQV 914

  Fly   928 SPYPVGYMNT--------------------PSPSTYSPNTPGGIPQSPYNPQTPGASLDSSMGDW 972
            :|.||||.||                    |||..|||.|||......|||.|||:::|.:..||
Zfish   915 APSPVGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSNIDQASNDW 979

  Fly   973 CTTDIEVRI-HTHDDTDLVGQTGIIRTVSNGVCSVFLRQEDRSVSIVSEHLAPVLPCNGDEFKII 1036
            .||||.||: .|..|..::.||||||:|:.|:|||||:..::.|||.||||.||.|...::.|:|
Zfish   980 VTTDIMVRVKDTFLDGGVINQTGIIRSVTGGMCSVFLQDTEKVVSISSEHLEPVTPTKNNKVKVI 1044

  Fly  1037 YGDDRESVGRVLSKDGDVFVCR--INEEIKLLPINFLCKMK 1075
            .|:|||:.|.:||.||:..:.|  ::|::|:|.:.||.|::
Zfish  1045 LGEDREATGVLLSIDGEDGIVRMELDEQLKILNLRFLGKLE 1085

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Spt5	NP_652610.1	Spt5_N	<136..208	CDD:288770	28/82 (34%)
		nusG	212..352	CDD:181467	103/139 (74%)
		NGN_Euk	215..302	CDD:193577	64/86 (74%)
		KOW_Spt5_1	313..350	CDD:240505	27/36 (75%)
		KOW_Spt5_2	461..511	CDD:240506	32/49 (65%)
		KOW_Spt5_3	512..562	CDD:240507	35/49 (71%)
		KOW_Spt5_4	638..680	CDD:240508	25/41 (61%)
		KOW_Spt5_5	737..788	CDD:240509	34/50 (68%)
		CTD	812..908	CDD:215026	59/111 (53%)
		CTD	812..870	CDD:289577	34/59 (58%)
		KOW_Spt5_6	1020..1075	CDD:240510	24/56 (43%)
supt5h	XP_005157586.1	Spt5_N	<93..172	CDD:314766	30/85 (35%)
		NGN_Euk	179..266	CDD:193577	64/86 (74%)
		KOW_Spt5_1	277..314	CDD:240505	27/36 (75%)
		CTD	335..965	CDD:331124	349/638 (55%)
		KOW_Spt5_6	1028..1084	CDD:240510	23/55 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170582040
Domainoid	1	1.000	547	1.000	Domainoid score	I252
eggNOG	1	0.900	-	-		E1_COG5164
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2384
Inparanoid	1	1.050	1088	1.000	Inparanoid score	I233
OMA	1	1.010	-	-		QHG54675
OrthoDB	1	1.010	-	-		D166902at33208
OrthoFinder	1	1.000	-	-		FOG0003457
OrthoInspector	1	1.000	-	-		oto40589
orthoMCL	1	0.900	-	-		OOG6_101219
Panther	1	1.100	-	-	LDO	PTHR11125
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1387
SonicParanoid	1	1.000	-	-		X2825
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	17	16.800