DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Spt5 and Supt5

DIOPT Version :9

Sequence 1:	NP_652610.1	Gene:	Spt5 / 53442	FlyBaseID:	FBgn0040273	Length:	1078	Species:	Drosophila melanogaster
Sequence 2:	NP_038704.1	Gene:	Supt5 / 20924	MGIID:	1202400	Length:	1082	Species:	Mus musculus

Alignment Length:	1138	Identity:	593/1138 - (52%)
Similarity:	773/1138 - (67%)	Gaps:	123/1138 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSDSEVSNMSDSGSEDGSISNKSQRSARSKSRSRSRSGSRGSRSVSRSRSRSQSGHSRSGSESPQ 65
            |||||.||.|:  .||...|:::: .|..:...||.:||
Mouse     1 MSDSEDSNFSE--EEDSERSSEAE-EAEVEEDQRSAAGS-------------------------- 36

  Fly    66 RRDNRGKSDESGEEEEEPPGEDIDSEEYDEEENDDHPRKKKKKERFGGFIIDEAEVDDEVDEDDE 130
                        |:||||..|:.:.|||||||.::...:..||.|.||||:|||:||||.:::|:
Mouse    37 ------------EKEEEPEEEEEEEEEYDEEEEEEDDDRPPKKPRHGGFILDEADVDDEYEDEDQ 89

  Fly   131 WEEGANEI----GIVGNEIDELGPTARDIEIRRRGTNLWDTQKEDEIEEYLRKKYADESIAKRHF 191
            ||:||.:|    .|..:.||.: ....|....||..|||..|:|:|:.||..||||..|:.:..:
Mouse    90 WEDGAEDILEKEEIEASNIDNV-VLDEDRSGARRLQNLWRDQREEELGEYYMKKYAKSSVGETVY 153

  Fly   192 GDGGEEMSDEITQQTLLPGIKDPNLWMVKCRIGEEKATALLLMRKYLTYLNTDDPLQIKSIIAPE 256
            | |.:|:||:||||.||||:||||||.|||:||||:|||:.||||::.|..||.||||||::|||
Mouse   154 G-GSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPE 217

  Fly   257 GVKGYIYLEAYKQTHVKTCIDNVGNLRMGKWKQEMVPIKEMTDVLKVVKEQVGLKVKQWVRLKRG 321
            .||||||:|||||||||..|:.|||||:|.|.|:||||||||||||||||...||.|.|||||||
Mouse   218 HVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVRLKRG 282

  Fly   322 LYKDDIAQVDYVDLAQNQVHLKLLPRIDYTRMRGALRTTATESDDSKRKKKRRPAAKPFDPEAVR 386
            :|||||||||||:.:||.:.||::|||||.|::..:   :.:...:||||.:||..:.||.|.:|
Mouse   283 IYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARM---SLKDWFAKRKKFKRPPQRLFDAEKIR 344

  Fly   387 AIGGEVHSDGDFLLFEGNRYSRKGFLYKNFTMSAILSDGVKPTLAELERFEESPEEVNLEIMGTV 451
            ::||:|.||||||:||||||||||||:|:|.|||::::||||||:|||:||:.||.::||:: |.
Mouse   345 SLGGDVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVV-TE 408

  Fly   452 KDDPTMAHSFSMGDNVEVCVGDLENLQAKIVAIDGTMITVMPKHQDLKDPLIFKASELRKYFKTG 516
            .......|:|..|||||||.|:|.|||.|::::||..||:||||:||||.|.|.|.|||||||.|
Mouse   409 STGKEREHNFQPGDNVEVCEGELINLQGKVLSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMG 473

  Fly   517 DHARVLAGRYEGETGLIIRVEPTRVVLVSDLTNHELEVLPRDLQLCSDVATGVDCLGQFQWGDMV 581
            ||.:|:|||:||:||||:|||...|:|.||||.|||:||||||||||:.|:|||..||.:||::|
Mouse   474 DHVKVIAGRFEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELV 538

  Fly   582 QLDSQNVGVIVRLERENFHVLGMNGKCIECKPTALHKRKENRHTVALDADQNQIRRRDVVKVMEG 646
            |||.:.||||||||||.|.||.|:||.:..:..|:.::|:||..||||:|||.|..:|:|||::|
Mouse   539 QLDPRTVGVIVRLERETFQVLNMHGKVVTVRHQAVTQKKDNRFAVALDSDQNNIHVKDIVKVIDG 603

  Fly   647 PHAGRSGEIKHLYRSLAFLHCRMYTENGGIFVCKTRHLQLAGGSK-TTVSNAGIVGGLGFMSPRI 710
            ||:||.|||:|||||.|||||:...||||:||||.|||.|||||| ..|:|. .|||...|||||
Mouse   604 PHSGREGEIRHLYRSFAFLHCKKLVENGGMFVCKARHLVLAGGSKPRDVTNL-TVGGFTPMSPRI 667

  Fly   711 QSPMHPS-----GGRGARGGARGGRGGFRVTRDREILGKTIKISGGPYKGAVGIVKDATESTARV 770
            .||||||     ||.|:.||...|||    .||.|::|:|::||.|||||.:|:|||||||||||
Mouse   668 SSPMHPSAEGQHGGFGSPGGMSRGRG----RRDNELIGQTVRISQGPYKGYIGVVKDATESTARV 728

  Fly   771 ELHTSCQTISVDRNHIAIVGVTGKEGSVSTYGRTPARTPGYGAQTPSYTAAGSKTPLVGSQTPNW 835
            |||::||||||||..:..|......|..|||||||.    ||:|||.| .:||:||:.|||||..
Mouse   729 ELHSTCQTISVDRQRLTTVDSQRPGGMTSTYGRTPM----YGSQTPMY-GSGSRTPMYGSQTPLQ 788

  Fly   836 DTDTRTP-YGTMTPSHDGSMTP-RHGAWDPTANTTPAR-NNDFDYSL-EEPSPSP-GY----NPS 891
            | .:||| ||:.||.||||.|| :.|||||....||:| ..:::|:. :||:||| .|    ||.
Mouse   789 D-GSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQ 852

  Fly   892 TPGY------QMTSQFAPQTPGT--LYGSDRSYSP---------FNPSPSPAP----SPYPVGYM 935
            ||||      |:..|:.||||||  :|.:|: :||         :.|||||..    :|.|.||.
Mouse   853 TPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQ-FSPYAAPSPQGSYQPSPSPQSYHQVAPSPAGYQ 916

  Fly   936 NT--------------------PSPSTYSPNTPGGIPQSPYNPQTPGASLDSSMGDWCTTDIEVR 980
            ||                    |||..|||.|||......|||.|||:.::.:..||.||||:|:
Mouse   917 NTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVK 981

  Fly   981 IH-THDDTDLVGQTGIIRTVSNGVCSVFLRQEDRSVSIVSEHLAPVLPCNGDEFKIIYGDDRESV 1044
            :. |:.||.:|||||:||:|:.|:|||:|:..::.|||.||||.|:.|...::.|:|.|:|||:.
Mouse   982 VRDTYLDTQIVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREAT 1046

  Fly  1045 GRVLSKDGDVFVCRI---NEEIKLLPINFLCKM 1074
            |.:||.||:..:.|:   :::||:|.:.||.|:
Mouse  1047 GVLLSIDGEDGIIRMDLEDQQIKILNLRFLGKL 1079

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Spt5	NP_652610.1	Spt5_N	<136..208	CDD:288770	29/75 (39%)
		nusG	212..352	CDD:181467	102/139 (73%)
		NGN_Euk	215..302	CDD:193577	63/86 (73%)
		KOW_Spt5_1	313..350	CDD:240505	26/36 (72%)
		KOW_Spt5_2	461..511	CDD:240506	31/49 (63%)
		KOW_Spt5_3	512..562	CDD:240507	35/49 (71%)
		KOW_Spt5_4	638..680	CDD:240508	28/41 (68%)
		KOW_Spt5_5	737..788	CDD:240509	35/50 (70%)
		CTD	812..908	CDD:215026	55/110 (50%)
		CTD	812..870	CDD:289577	34/59 (58%)
		KOW_Spt5_6	1020..1075	CDD:240510	23/58 (40%)
Supt5	NP_038704.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..88		43/127 (34%)
		Spt5_N	<91..169	CDD:288770	32/79 (41%)
		nusG	173..314	CDD:181467	102/140 (73%)
		Interaction with SUPT4H1 and SUPT4H2. /evidence=ECO:0000250	174..268		70/93 (75%)
		NGN_Euk	176..263	CDD:193577	63/86 (73%)
		KOW_Spt5_1	274..311	CDD:240505	26/36 (72%)
		Interaction with RNA polymerase II. /evidence=ECO:0000250	311..418		55/110 (50%)
		SPT5	332..959	CDD:227493	350/639 (55%)
		KOW_Spt5_2	418..468	CDD:240506	31/49 (63%)
		KOW_Spt5_3	469..519	CDD:240507	35/49 (71%)
		KOW_Spt5_4	595..637	CDD:240508	28/41 (68%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	669..694		14/28 (50%)
		KOW_Spt5_5	696..745	CDD:240509	34/48 (71%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	741..972		98/237 (41%)
		9 X 7 AA approximate tandem repeats of G-S-[QR]-T-P-X-[YQ], motif CTR1	748..811		37/68 (54%)
		CTD	760..816	CDD:289577	35/61 (57%)
		10 X 8 AA approximate tandem repeats of P-[TS]-P-S-P-[QA]-[SG]-Y, motif CTR2	838..944		37/106 (35%)
		Caudal_act	841..970	CDD:282574	47/129 (36%)
		KOW_Spt5_6	1022..1079	CDD:240510	22/56 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167838201
Domainoid	1	1.000	539	1.000	Domainoid score	I272
eggNOG	1	0.900	-	-		E1_COG5164
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2384
Inparanoid	1	1.050	1096	1.000	Inparanoid score	I237
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54675
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003457
OrthoInspector	1	1.000	-	-		oto92082
orthoMCL	1	0.900	-	-		OOG6_101219
Panther	1	1.100	-	-	LDO	PTHR11125
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1387
SonicParanoid	1	1.000	-	-		X2825
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	16	15.790