DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Spt5 and spt-5

DIOPT Version :9

Sequence 1:	NP_652610.1	Gene:	Spt5 / 53442	FlyBaseID:	FBgn0040273	Length:	1078	Species:	Drosophila melanogaster
Sequence 2:	NP_502283.1	Gene:	spt-5 / 178143	WormBaseID:	WBGene00005015	Length:	1208	Species:	Caenorhabditis elegans

Alignment Length:	1257	Identity:	458/1257 - (36%)
Similarity:	662/1257 - (52%)	Gaps:	244/1257 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 SGSEDGSISNKSQRSARSKSRSRSRSGSRGSRSVSRSRSRSQSGHSRSGSESPQRRDNRGKSDES 76
            |..|..:.|||.:.:..|..                           .||:...:...:.:..:.
Worm     2 SSDESDAASNKEEDTTLSDD---------------------------DGSDVEIKNSKKDRKRKL 39

  Fly    77 GEEEEEPPGEDIDSEEYDEEENDDHPRKKKKKERFGG-----FI-----IDEAEVDDEVDEDDEW 131
            ..|.|:...:|.|.::.||:||....:.||:|.:...     |:     :|:.:.||:.|.|||.
Worm    40 ASESEKSGSDDDDDDDDDEDENVTSKKSKKQKRKNKAPSGRDFLAWDVEVDDEDEDDDNDYDDED 104

  Fly   132 EEGANEIGIVGNEIDELGPTARDIEIRRRGTNLWDTQK--EDEIEEYLRKKYADESIAKRHFGDG 194
            :...|.:    ||.:|.....:::|:.:|..:.:..|.  |||:::|...||..:....::  |.
Worm   105 DPSMNAM----NEREEAERAMKEMELSQRNRDRYKFQNMTEDEVQKYFENKYKGDKNDSQY--DD 163

  Fly   195 GEEMSDEITQQTLLPGIKDPNLWMVKCRIGEEKATALLLMRKYLTYLNTDDPLQIKSIIAPEGVK 259
            .:...|:|::.:.||..||||||:||||:||||..|:.||||.|...:|::|.||||::..||:|
Worm   164 EDSAMDDISKNSHLPSTKDPNLWIVKCRMGEEKLVAMHLMRKCLAVEHTNEPFQIKSVVVKEGLK 228

  Fly   260 GYIYLEAYKQTHVKTCIDNVGNLRMGKWKQEMVPIKEMTDVLKVVKEQVGLKVKQWVRLKRGLYK 324
            |.||:||:||:||.:.|:  |...:.::...|||||:|.|||:|||:...||:..:|||||.:||
Worm   229 GMIYIEAFKQSHVMSAIE--GFSALNQFTITMVPIKDMVDVLRVVKDIPQLKLGSYVRLKRTMYK 291

  Fly   325 DDIAQVDYVDLAQNQVHLKLLPRIDYTRMRGALRTTATESDDSKRKKKRRPAAKPFDPEAVRAIG 389
            ||:|.||.||:|||:|:|||:||:||.:.|||:||.|    |...|.||||..|.||.:.::.:|
Worm   292 DDLAVVDLVDIAQNRVNLKLIPRVDYQKRRGAMRTDA----DKNYKLKRRPMPKLFDQDTIKEVG 352

  Fly   390 GEVHSDGDFLLFEGNRYSRKGFLYKNFTMSAILSDGVKPTLAELERFEESPEEVNLEI-MGTVKD 453
            ||:.:|||||:||||.: |:|||||.|.::||.:|||||||.|||:|:||.:::..|: ..::||
Worm   353 GEIVTDGDFLVFEGNHF-RRGFLYKYFPINAIQADGVKPTLGELEKFQESSDDLKRELETASLKD 416

  Fly   454 DPTMAHSFSMGDNVEVCVGDLENLQAKIVAIDGTMITVMPKHQDLKDPLIFKASELRKYFKTGDH 518
               ..:.|..||.|||..|:|.||:.|::.:|||.:.:||..:|||:.:...|.|||||||.|||
Worm   417 ---TENPFVPGDIVEVKAGELVNLRGKVMTVDGTKVVMMPDQEDLKEAITLNAHELRKYFKEGDH 478

  Fly   519 ARVLAGRYEGETGLIIRVEPTRVVLVSDLTNHELEVLPRDLQLCSDVATGVDCLGQFQWGDMVQL 583
            |:|::|||||.||||:||:.:..:::|||...||:|..||||||:||.||||.|||||:.|:|||
Worm   479 AKVISGRYEGHTGLIVRVKDSTAIVLSDLGMEELKVRVRDLQLCADVTTGVDSLGQFQYHDLVQL 543

  Fly   584 D-SQNVGVIVRLERENFHVLGMNGKCIECKPTALHKRKENRHTVALDADQNQIRRRDVVKVMEGP 647
            | :.|||||||||:|:..||.|:|.....||.|:..:|:.|....||:..|.|..:|:|||:.||
Worm   544 DHTGNVGVIVRLEKEHLEVLNMHGVVNRIKPQAIIAKKDVRFAKVLDSQNNSIEAKDLVKVIGGP 608

  Fly   648 HAGRS-------GEIKHLYRSLAFLHCRMYTENGGIFVCKTRHLQLAGGSKTTVSNAGIVGGLGF 705
            :|...       ||:.:.:|...|::.|..|:|||:.|||.:.|.|.|..|||  :..:|..:..
Worm   609 NAKERETDEDPVGEVLYAFRGTVFVYSRKVTKNGGVLVCKPKQLILQGAKKTT--STPMVSRMAS 671

  Fly   706 MSPRIQSPMHPSGG------------RGARG------GARGGRGGF-------RVTRDREILGKT 745
            .:| :.||.|.|||            ||:.|      |..|||||.       :|.||..::||.
Worm   672 PNP-MASPRHSSGGMTPRSPQDGMSSRGSSGGQTPRQGGGGGRGGHFGTANQQKVRRDLTLIGKN 735

  Fly   746 IKISGGPYKGAVGIVKDATESTARVELHTSCQTISVDRNHIAIVGVT----GKEGSVSTY----- 801
            ::|..||.||..|||:||||.|.||||||.|:||||||..:.:||.|    |..|..|.|
Worm   736 VRIIKGPMKGHFGIVRDATEDTVRVELHTQCRTISVDRARVMVVGDTGITAGSGGGSSFYSSSKT 800

  Fly   802 -----GRTP---ARTPGYGAQTPSY-----------TAA---GSKTPLVGSQTPNW-DTD----T 839
                 |:||   ::||.||||||.|           |.|   |.:||..||:||.: |.|    :
Worm   801 PMRDSGKTPMYGSKTPMYGAQTPMYGSMTPAYDGGRTPAYGEGGRTPAYGSKTPAYGDLDEHSSS 865

  Fly   840 RTP-YG---TMTPSH---DGSMTPRHG-------------------AWDPTANT----------- 867
            ||| ||   :.||::   ||:.||.:|                   |:|.:..|
Worm   866 RTPAYGNDSSRTPAYGSADGARTPAYGSTEGGRTPAYGSMDNSRTPAYDDSGRTPGYESMPSRTP 930

  Fly   868 -------------------TPARNNDFDYSLEEPSPSPGYNPSTP-GYQ----MTSQFAPQTPG- 907
                               |||.|||:|..|     ||.|.|..| .|.    .|..|..:|||
Worm   931 NYDSSSKTPAYPESEHSARTPAYNNDYDIPL-----SPAYEPDAPEAYDNAPARTPAFVSRTPGY 990

  Fly   908 -TLYGSDRSYSP-----------------FNPSPSPAPSPYPVGYMNTPSPSTYSPNTPG-GIPQ 953
             |...|..:|.|                 ::..|.....|.|...:|..:|..|..:||| ..|.
Worm   991 DTYENSSPTYEPDAATKVEEDIGDTSSPTYDSPPHSYVVPTPGAMLNPATPGAYHVDTPGFAAPM 1055

  Fly   954 SP--------YNPQTPGASLDS------------------SMGDWCTTDIEVRIHTHDDTDLVGQ 992
            :|        |...:|.|..||                  :.|.|...::.|:|..|:.. ..|:
Worm  1056 TPGSGGAYDQYVAPSPFAGYDSNNYNNADGAIEQIPDHFLAQGVWIVQNLCVQIKDHEGR-FNGR 1119

  Fly   993 TGIIRTVSNGVCSVFLRQEDRSVSIVSEHLAPVLPCNGDEFKIIYGDDRESVGRVLSKDGDVFVC 1057
            ..||:.|::|...|::.....::.:..:.|.|:.|..||:.::|:|.|....|:::|.||...|.
Worm  1120 EAIIKDVTDGKVDVYMPDHKCNLEVDFDQLTPMKPQPGDDARVIFGQDAGHSGQLVSMDGFEAVI 1184

  Fly  1058 RINE---EIKLLPINFLCKMKS 1076
            |..|   :::::.|...|||.|
Worm  1185 RSQEDMSDMRVINIGLCCKMHS 1206

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Spt5	NP_652610.1	Spt5_N	<136..208	CDD:288770	16/73 (22%)
		nusG	212..352	CDD:181467	79/139 (57%)
		NGN_Euk	215..302	CDD:193577	45/86 (52%)
		KOW_Spt5_1	313..350	CDD:240505	23/36 (64%)
		KOW_Spt5_2	461..511	CDD:240506	22/49 (45%)
		KOW_Spt5_3	512..562	CDD:240507	29/49 (59%)
		KOW_Spt5_4	638..680	CDD:240508	17/48 (35%)
		KOW_Spt5_5	737..788	CDD:240509	30/50 (60%)
		CTD	812..908	CDD:215026	51/177 (29%)
		CTD	812..870	CDD:289577	33/132 (25%)
		KOW_Spt5_6	1020..1075	CDD:240510	18/57 (32%)
spt-5	NP_502283.1	Spt5_N	96..177	CDD:288770	21/86 (24%)
		NGN_Euk	184..269	CDD:193577	45/86 (52%)
		KOW_Spt5_1	280..317	CDD:240505	23/36 (64%)
		SPT5	337..1032	CDD:227493	279/706 (40%)
		KOW_Spt5_2	421..471	CDD:240506	22/49 (45%)
		KOW_Spt5_3	472..522	CDD:240507	29/49 (59%)
		KOW_Spt5_4	599..648	CDD:240508	17/48 (35%)
		KOW_Spt5_5	726..778	CDD:240509	30/51 (59%)
		CTD	850..924	CDD:289577	21/73 (29%)
		CTD	896..967	CDD:289577	14/75 (19%)
		CTD	935..1001	CDD:289577	21/70 (30%)
		KOW_Spt5_6	1147..1205	CDD:240510	18/57 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160160059
Domainoid	1	1.000	211	1.000	Domainoid score	I1631
eggNOG	1	0.900	-	-		E1_COG5164
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2384
Inparanoid	1	1.050	671	1.000	Inparanoid score	I380
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54675
OrthoDB	1	1.010	-	-		D166902at33208
OrthoFinder	1	1.000	-	-		FOG0003457
OrthoInspector	1	1.000	-	-		oto19463
orthoMCL	1	0.900	-	-		OOG6_101219
Panther	1	1.100	-	-	LDO	PTHR11125
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1387
SonicParanoid	1	1.000	-	-		X2825
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.800