DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ACXB and Adcy8

DIOPT Version :9

Sequence 1:	NP_620474.2	Gene:	ACXB / 53427	FlyBaseID:	FBgn0040509	Length:	1114	Species:	Drosophila melanogaster
Sequence 2:	NP_058838.1	Gene:	Adcy8 / 29241	RGDID:	2036	Length:	1248	Species:	Rattus norvegicus

Alignment Length:	1154	Identity:	323/1154 - (27%)
Similarity:	523/1154 - (45%)	Gaps:	228/1154 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 ERLWERGYLKAKCKELCLEEEYKKYQIRLMISYLTVFFPLFIFVV-VGCELCPWIFSEQKKLIYY 82
            |||::|.:|..:.|.            .::::.|.|...|.:.|: :.....|   .:..|.|..
  Rat   164 ERLYQRYFLGQRRKS------------EVVMNVLDVLTKLTLLVLHLSLASAP---MDPLKGILL 213

  Fly    83 ESLTAIVVLVAVPGILSINFFESFVLRH----RWIMVFSSILSAYI--VVLVDIFQILVFHFKYH 141
            ...|.|.|::....::..:......|::    .|:.:.:.||:|.:  .:|.|
  Rat   214 GFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGD------------ 266

  Fly   142 WPLNTLYDVFVLCMIYMFLPIPSIRGAALLATSVSLMYIGLFIYFLKSDDEDISEVVHDLDTICV 206
               ...|.:|.|...|..||:| :..|.|.....||:.:.|.:...:.       .|..::.:..
  Rat   267 ---GIGYVLFTLFATYSMLPLP-LTWAILAGLGTSLLQVTLQVLIPRL-------AVFSINQVLA 320

  Fly   207 DIFHYLGFNLMGIFFRIMNDTMVRSSFLDRHQFLKEEMWLRQARQQESMLLDSILPPQIAKPIQQ 271
            .:..::..|..|||...::|...|.:||:..:.::..:.|....|::..|:.|:||
  Rat   321 QVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLP--------- 376

  Fly   272 SIKERIMLSETDSDRIVVNARRTEN-FMAIQIH--PDVSILYADVVNYTHLTTTLTVEKLVKVLH 333
                |.::.|..:|...|.....:: |..|.||  .:||||:|||..:|:|:|||:.::||::|:
  Rat   377 ----RFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLN 437

  Fly   334 DLYGRFDMAASTFKVQRIKFLGDCYYCVAGLGEADPDHARMAVSLGISMIANIQEVRAHRALDID 398
            :|:.|||..|......|||.||||||||:||.|...|||...|.:|:|||..|:.||:....|:|
  Rat   438 ELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVD 502

  Fly   399 MRIGVHSGTLLAGVIGQAKLQYDIWGPDVDIANRLEATGKPGYVHVSGRTLSSLNVAEYTVFPGT 463
            ||||:|||::|.||:|..|.|:|:|..||||||:||:.|.||.:|:|..||..|: .:|.|..|
  Rat   503 MRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLS-GDYNVEEG- 565

  Fly   464 EVAQSDPILQKQPMTTYL-------LTAAPS---RNSVRSVDAVHSYA---------EIDI---- 505
            ...:.:..|:|..:.|||       |.:.|.   :.||...|..:|.|         |:..
  Rat   566 HGKERNEFLRKHNIETYLIKQPEESLLSLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNIV 630

  Fly   506 ---NALGA-SRKSPILRPTLMSDELR-----EEFKKMPVGGFNIRSPCCRDNSNDEKVNHGLGMF 561
               |.|.| :|.|..|.|..::..|.     ||..|......::|       |.|:.....:..|
  Rat   631 GKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLR-------SGDKLRREHIKPF 688

  Fly   562 CVAFKDSSLEWSYLHKPDFILKYSVLLAWGIGCCLIYIQIVNDKFTICVECIVIDMVV-FTFL-- 623
            .:.|||||||..|....|.:.|.:::.|:.:   |::|..:.   ::.....::.|.: |:.|
  Rat   689 SLMFKDSSLEHKYSQMRDEVFKSNLVCAFIV---LLFITAIQ---SLLPSSRLMPMTIQFSILIM 747

  Fly   624 --TSLLFISW----------FKKVCWWRCVENDSRKYGRVSCKLFKIWERIQHSFVLR-VTIYMS 675
              ::|:.|:.          .:|.|.|                       |..:::.| |.|:.|
  Rat   748 LHSALVLITTAEDYKCLPLILRKTCCW-----------------------INETYLARNVIIFAS 789

  Fly   676 IVASYYLLISTILLNCDKNQYELDVINSKLYHYDIVTDNCFNP--WVFTDMISLIVGVSYTFARI 738
            |:.::...:..||. ||.:: .:.:.|.......:.||.|..|  :|||.:::::  ....|.|:
  Rat   790 ILINFLGAVINILW-CDFDK-SIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMV--TCAVFLRL 850

  Fly   739 PFALKMLITCCATVAYLVIVFFQYSFIF------EHSATTTPFMKAELAHCLRVCMMLLTMYAKE 797
            ...||:.:.......|.::....|:.:|      .||.  ..|:..:.|..|.:.|.||.::...
  Rat   851 NSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSG--EDFLGTKEASLLLMAMFLLAVFYHG 913

  Fly   798 RQSEFNTKINFKINQDLQGKQKAADIT-----NKSIIILLTNILPSHVVEVYLDSVA-NHELYYE 856
            :|.|:..:::|...  :|.|::..::.     |::   :|.||||.||...:|:... |.|||.:
  Rat   914 QQLEYTARLDFLWR--VQAKEEINEMKDLREHNEN---MLRNILPGHVARHFLEKDRDNEELYSQ 973

  Fly   857 NYKMVSVMFAMLINF----------QMDLPSLRVLNDIITEFDRLLNAYKEYYVVEKIKVVGCTY 911
            :|..|.||||.:..|          ...:..||:||:||.:||.||.. ..:..:||||.:|.||
  Rat   974 SYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGE-DRFQDIEKIKTIGSTY 1037

  Fly   912 MAACGLDFTLAKSKFGSRTHASYSSELEQVLYRKESKGTENDHDEVAFI--MTTFALDLMRVLSV 974
            ||..||                 |.|.:|.            .|:...:  :..|:|.|...:..
  Rat  1038 MAVSGL-----------------SPEKQQC------------EDKWGHLCALADFSLALTESIQE 1073

  Fly   975 CNKAYAGRPFDRALSTGEICIGISTGEIMAGVVGASQPHYDIWGNPVNMASRMESTGLPGHIHVT 1039
            .||        .:.:..|:.||||.|.::|||:||.:|.|||||..||:||||:|||:.|.|.|.
  Rat  1074 INK--------HSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVP 1130

  Fly  1040 QETANILEQFDIMCMYRGMTFVKG----RGEIPTYFV--GIDDNLKFLP 1082
            :||..||:.......|||..:|||    .|:|.|||:  .:..|...||
  Rat  1131 EETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILP 1179

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ACXB	NP_620474.2	AC_N	<36..276	CDD:292831	44/246 (18%)
		CYCc	249..459	CDD:214485	92/212 (43%)
		Nucleotidyl_cyc_III	298..484	CDD:299850	90/194 (46%)
		CYCc	825..1047	CDD:214485	82/234 (35%)
		Nucleotidyl_cyc_III	853..1072	CDD:299850	82/234 (35%)
Adcy8	NP_058838.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000269\|PubMed:16258073, ECO:0000269\|PubMed:22494970, ECO:0000269\|PubMed:22976297	1..179		6/26 (23%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000269\|PubMed:22976297	1..106
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..117
		AC_N	<158..397	CDD:292831	54/283 (19%)
		CYCc	363..561	CDD:214485	91/211 (43%)
		Guanylate_cyc	402..586	CDD:278633	89/185 (48%)
		DUF1053	614..708	CDD:283888	25/100 (25%)
		CYCc	941..1145	CDD:214485	83/244 (34%)
		Guanylate_cyc	970..1169	CDD:278633	83/236 (35%)
		Involved in CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	1106..1248		34/74 (46%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000269\|PubMed:19305019	1197..1212
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1220..1248
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810