DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ACXE and Adcy8

DIOPT Version :9

Sequence 1:	NP_652601.3	Gene:	ACXE / 53426	FlyBaseID:	FBgn0040506	Length:	1123	Species:	Drosophila melanogaster
Sequence 2:	NP_058838.1	Gene:	Adcy8 / 29241	RGDID:	2036	Length:	1248	Species:	Rattus norvegicus

Alignment Length:	1249	Identity:	334/1249 - (26%)
Similarity:	546/1249 - (43%)	Gaps:	292/1249 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 GYLKAKCAELRLESEFRL-----YR----------------IRLWKSYL---------------- 48
            |:|...||.  ..|:|.|     ||                .||::.|.
  Rat   125 GFLHLDCAP--SNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDV 187

  Fly    49 ---LTFFMLHIFVTSVHC----ALLLATIERRSIIYFDVALSIGCALVLILVLSVNFCDEFIAKH 106
               ||..:||:.:.|...    .:||.        :|.....:.||||::        .:....|
  Rat   188 LTKLTLLVLHLSLASAPMDPLKGILLG--------FFTGIEVVICALVVV--------RKDTTSH 236

  Fly   107 TWYMYASSIFASLTLVFADLTESIYHTYAHSWIL--GTFYDTYIIYMIYMFLPIHFISGAVLLAL 169
            |:..|:.      .:.:..:|..|........:|  |..|..:.::..|..||:. ::.|:|..|
  Rat   237 TYLQYSG------VVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLP-LTWAILAGL 294

  Fly   170 LVSGLYILYFVIFIAQGFAQFASALFSVGGMSVDIVHYLCLNLVGIFYRVMNDTVVRSSFLDRHQ 234
            ..|.|.:...|:..       ..|:||:..:...:|.::|:|..|||...::|...|.:||:..:
  Rat   295 GTSLLQVTLQVLIP-------RLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRR 352

  Fly   235 YIKEKIWLRNARLQEKQLLDSILPPQISLPLQKDIQGRIVMAKQGIHSWTAMERTMAIQIH--PD 297
            .::.::.|.....::::|:.|:||..:.|.:..|:        ..:.......:...|.||  .:
  Rat   353 CVEARLRLETENQRQERLVLSVLPRFVVLEMINDM--------TNVEDEHLQHQFHRIYIHRYEN 409

  Fly   298 VSILYADVVNYTHLTTTLTVEMLVKVLHDLYGRFDLAAYRYKVQRIKFLGDCYYCVAGLSDPDPD 362
            ||||:|||..:|:|:|||:.:.||::|::|:.|||..|:.:...|||.||||||||:||.:|..|
  Rat   410 VSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQD 474

  Fly   363 HANNCVILGLSMINHIMEVRDIHGLDINMRIGVHSGNLFAGVIGEAKLQFDIWGLDVTIANVLES 427
            ||:.||.:|||||..|..||.....|::||||:|||::..||:|..|.|||:|..||.|||.|||
  Rat   475 HAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLES 539

  Fly   428 TGVPGCVHISGATLNNLDVNRFDIEDGPEEARDHPLLKKYRIRSYIIRQDLHMDDEDS------- 485
            .|:||.:|||.|||:.|. ..:::|:|..:.|:. .|:|:.|.:|:|:|     .|:|
  Rat   540 GGIPGRIHISKATLDCLS-GDYNVEEGHGKERNE-FLRKHNIETYLIKQ-----PEESLLSLPED 597

  Fly   486 -----------------------------DEFLGDLHSISLCNMGAQPRISDSANQSLRALFHEE 521
                                         |..:|..::::.....:   |:...|...:||..:.
  Rat   598 IVKESVSCSDRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNS---INLLPNHLAQALHVQS 659

  Fly   522 LREEFRKMPVSAFSPKRLLGICRFNTGKEVPAHQNLNICLTFTDPILERAYLKQTDYMYKYSIIL 586
            ..||..         ||:.......:|.::.........|.|.|..||..|.:..|.::|.:::.
  Rat   660 GPEEIN---------KRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVC 715

  Fly   587 SASVGCSLVYIELMDTQMICS----------------SCFVLPASVATIQCILALIAWYKKYCW- 634
            :..|   |::|..:.:.:..|                |..||..:....:|:..::.  |..||
  Rat   716 AFIV---LLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILR--KTCCWI 775

  Fly   635 --TRYGRNNVPHHYNGFSCFIFRIHDKILN--------SLPIRICIYLFLMISSFFVMCLIVMSC 689
              |...||.:.     |:..:......::|        |:|::
  Rat   776 NETYLARNVII-----FASILINFLGAVINILWCDFDKSIPLK---------------------- 813

  Fly   690 QREEFEMAYIEERLFHYEQEAHICFHP--WVTTNMLSLMICLTFTFAHIPIMVKTAVAILETLAY 752
                 .:.:....:|     ..||.:|  :|.|.:|:::.|.  .|..:..::|.||.::....|
  Rat   814 -----NLTFNSSAVF-----TDICSYPEYFVFTGVLAMVTCA--VFLRLNSVLKLAVLLIMIAIY 866

  Fly   753 LLL-------IFFQFDFVFHHSVTTNPYFKSEYAHALLICITFLIMFVKERQIEFTNKVNFNWRV 810
            .||       :|..:|.:.|   :...:..::.|..||:.:..|.:|...:|:|:|.:::|.|||
  Rat   867 ALLTETIYAGLFLSYDNLNH---SGEDFLGTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRV 928

  Fly   811 DLRKEEN-AASLTNHSIIIILNNILPSHIVDVYL-NSLAKHELYFENYRMVSVMFAMLINF---- 869
            ..::|.| ...|..|: ..:|.||||.|:...:| ......|||.::|..|.||||.:..|
  Rat   929 QAKEEINEMKDLREHN-ENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFY 992

  Fly   870 ---EMD---LRSLRVLNEIIAEFDTLLLFYKEYYTVEKIKIVGCTYMAACGLDLNFAGSTSTNRK 928
               ||:   :..||:||||||:||. ||....:..:||||.:|.||||..||...
  Rat   993 SQTEMNNQGVECLRLLNEIIADFDE-LLGEDRFQDIEKIKTIGSTYMAVSGLSPE---------- 1046

  Fly   929 ESIPPTEFNEEQSRRILFQQSNEDLDEVVFVMTSYALDMMRTLAKSNEAYQSIAGDRNITDGTIA 993
                              :|..||....:..:..::|.:..::.:.|:        .:..:..:.
  Rat  1047 ------------------KQQCEDKWGHLCALADFSLALTESIQEINK--------HSFNNFELR 1085

  Fly   994 IGISSGEVMAGIVGASQPHYDIWGNPVNMASRMESTGLPGHIHVTEETSEILQQFGITCSYRGMT 1058
            ||||.|.|:||::||.:|.|||||..||:||||:|||:.|.|.|.|||..||:..|....|||..
  Rat  1086 IGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEI 1150

  Fly  1059 FVKG----RGKIPTY-LVG-IDENLNFIPQKATRFPSHQERSTVI--SLQSTYTHA------ENN 1109
            :|||    .|||.|| |:| :..|...:|.:  |.|.....:.|:  .:||.....      ||:
  Rat  1151 YVKGISEQEGKIKTYFLLGRVQPNPFILPPR--RLPGQYSLAAVVLGLVQSLNRQRQKQLLNENS 1213

  Fly  1110 NSIASTSRHTLQSL 1123
            ||....|.:..::|
  Rat  1214 NSGIIKSHYNRRTL 1227

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ACXE	NP_652601.3	AC_N	<31..272	CDD:318454	57/286 (20%)
		Nucleotidyl_cyc_III	290..459	CDD:325147	86/170 (51%)
		Nucleotidyl_cyc_III	851..1071	CDD:325147	84/234 (36%)
Adcy8	NP_058838.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000269\|PubMed:16258073, ECO:0000269\|PubMed:22494970, ECO:0000269\|PubMed:22976297	1..179		12/55 (22%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000269\|PubMed:22976297	1..106
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..117
		AC_N	<158..397	CDD:292831	52/276 (19%)
		CYCc	363..561	CDD:214485	90/206 (44%)
		Guanylate_cyc	402..586	CDD:278633	92/185 (50%)
		DUF1053	614..708	CDD:283888	18/105 (17%)
		CYCc	941..1145	CDD:214485	82/241 (34%)
		Guanylate_cyc	970..1169	CDD:278633	85/235 (36%)
		Involved in CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	1106..1248		48/124 (39%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000269\|PubMed:19305019	1197..1212		2/14 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1220..1248		2/8 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810