DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ACXE and Adcy8

DIOPT Version :9

Sequence 1:	NP_652601.3	Gene:	ACXE / 53426	FlyBaseID:	FBgn0040506	Length:	1123	Species:	Drosophila melanogaster
Sequence 2:	NP_033753.2	Gene:	Adcy8 / 11514	MGIID:	1341110	Length:	1249	Species:	Mus musculus

Alignment Length:	1244	Identity:	334/1244 - (26%)
Similarity:	548/1244 - (44%)	Gaps:	282/1244 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 GYLKAKCAELRLESEFRL-----YR----------------IRLWKSYL---------------- 48
            |:|...||.  ..|:|.|     ||                .||::.|.
Mouse   126 GFLHLDCAP--SNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDV 188

  Fly    49 ---LTFFMLHIFVTSVHC----ALLLATIERRSIIYFDVALSIGCALVLILVLSVNFCDEFIAKH 106
               ||..:||:.:.|...    .:||.        :|.....:.||||::...:.:        |
Mouse   189 LTKLTLLVLHLSLASAPMDPLKGILLG--------FFTGIEVVICALVVVRKDNTS--------H 237

  Fly   107 TWYMYASSIFASLTLVFADLTESIYHTYAHSWIL--GTFYDTYIIYMIYMFLPIHFISGAVLLAL 169
            |:..|:.      .:.:..:|..|........:|  |..|..:.::..|..||:. ::.|:|..|
Mouse   238 TYLQYSG------VVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLP-LTWAILAGL 295

  Fly   170 LVSGLYILYFVIFIAQGFAQFASALFSVGGMSVDIVHYLCLNLVGIFYRVMNDTVVRSSFLDRHQ 234
            ..|.|.:...|:..       ..|:||:..:...:|.::|:|..|||...::|...|.:||:..:
Mouse   296 GTSLLQVTLQVLIP-------RLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRR 353

  Fly   235 YIKEKIWLRNARLQEKQLLDSILPPQISLPLQKDIQGRIVMAKQGIHSWTAMERTMAIQIH--PD 297
            .::.::.|.....::::|:.|:||..:.|.:..|:        ..:.......:...|.||  .:
Mouse   354 CVEARLRLETENQRQERLVLSVLPRFVVLEMINDM--------TNVEDEHLQHQFHRIYIHRYEN 410

  Fly   298 VSILYADVVNYTHLTTTLTVEMLVKVLHDLYGRFDLAAYRYKVQRIKFLGDCYYCVAGLSDPDPD 362
            ||||:|||..:|:|:|||:.:.||::|::|:.|||..|:.:...|||.||||||||:||.:|..|
Mouse   411 VSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRRD 475

  Fly   363 HANNCVILGLSMINHIMEVRDIHGLDINMRIGVHSGNLFAGVIGEAKLQFDIWGLDVTIANVLES 427
            ||:.||.:|||||..|..||.....|::||||:|||::..||:|..|.|||:|..||.|||.|||
Mouse   476 HAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLES 540

  Fly   428 TGVPGCVHISGATLNNLDVNRFDIEDGPEEARDHPLLKKYRIRSYIIRQDLHMDDEDS------- 485
            .|:||.:|||.|||:.|: ..:::|:|..:.|:. .|:|:.|.:|:|:|     .|:|
Mouse   541 GGIPGRIHISKATLDCLN-GDYNVEEGHGKERNE-FLRKHNIETYLIKQ-----PEESLLCLPED 598

  Fly   486 -----------------------------DEFLGDLHSISLCNMGAQPRISDSANQSLRALFHEE 521
                                         |..:|..::::.....:   |:...|...:||..:.
Mouse   599 IVKESVSCSDRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNS---INLLPNHLAQALHVQS 660

  Fly   522 LREEFRKMPVSAFSPKRLLGICRFNTGKEVPAHQNLNICLTFTDPILERAYLKQTDYMYKYSIIL 586
            ..||..         ||:.......:|.::.........|.|.|..||..|.:..|.::|.:::.
Mouse   661 GPEEIN---------KRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVC 716

  Fly   587 SASVGCSLVYIELMDTQMICS----------------SCFVLPASVATIQCILALIAWYKKYCW- 634
            :..|   |::|..:.:.:..|                |..||..:....:|:..::.  |..||
Mouse   717 AFIV---LLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILR--KTCCWI 776

  Fly   635 --TRYGRNNVPHHYNGFSCFIFRIHDKILNSLPIRICIYLFLMISSFFVMCLIVMSCQREEF--- 694
              |...||                           :.|:..::| :|....|.::.|..::.
Mouse   777 NETYLARN---------------------------VIIFASILI-NFLGAVLNILWCDFDKSIPL 813

  Fly   695 -EMAYIEERLFHYEQEAHICFHP--WVTTNMLSLMICLTFTFAHIPIMVKTAVAILETLAYLLL- 755
             .:.:....:|     ..||.:|  :|.|.:|:::.|.  .|..:..::|.||.::....|.||
Mouse   814 KNLTFNSSAVF-----TDICSYPEYFVFTGVLAMVTCA--VFLRLNSVLKLAVLLIMIAIYALLT 871

  Fly   756 ------IFFQFDFVFHHSVTTNPYFKSEYAHALLICITFLIMFVKERQIEFTNKVNFNWRVDLRK 814
                  :|..:|.:.|   :...:..::.|..||:.:..|.:|...:|:|:|.:::|.|||..::
Mouse   872 ETIYAGLFLSYDNLNH---SGEDFLGTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKE 933

  Fly   815 EENAASLTNHSIIIILNNILPSHIVDVYL-NSLAKHELYFENYRMVSVMFAMLINF-------EM 871
            |.|...........:|.||||||:...:| ......|||.::|..|.||||.:..|       ||
Mouse   934 EINEMKELREHNENMLRNILPSHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEM 998

  Fly   872 D---LRSLRVLNEIIAEFDTLLLFYKEYYTVEKIKIVGCTYMAACGLDLNFAGSTSTNRKESIPP 933
            :   :..||:||||||:||. ||....:..:||||.:|.||||..||...
Mouse   999 NNQGVECLRLLNEIIADFDE-LLGEDRFQDIEKIKTIGSTYMAVSGLSPE--------------- 1047

  Fly   934 TEFNEEQSRRILFQQSNEDLDEVVFVMTSYALDMMRTLAKSNEAYQSIAGDRNITDGTIAIGISS 998
                         :|..||....:..:..::|.:..::.:.|:        .:..:..:.||||.
Mouse  1048 -------------KQQCEDKWGHLCALADFSLALTESIQEINK--------HSFNNFELRIGISH 1091

  Fly   999 GEVMAGIVGASQPHYDIWGNPVNMASRMESTGLPGHIHVTEETSEILQQFGITCSYRGMTFVKG- 1062
            |.|:||::||.:|.|||||..||:||||:|||:.|.|.|.|||..||:..|....|||..:|||
Mouse  1092 GSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGI 1156

  Fly  1063 ---RGKIPTY-LVG-IDENLNFIPQKATRFPSHQERSTVI--SLQSTYTHA------ENNNSIAS 1114
               .|||.|| |:| :..|...:|.:  |.|.....:.|:  .:||.....      ||:||...
Mouse  1157 SEQEGKIKTYFLLGRVQPNPFILPPR--RLPGQYSLAAVVLGLVQSLNRQRQKQLLNENSNSGII 1219

  Fly  1115 TSRHTLQSL 1123
            .|.:..::|
Mouse  1220 KSHYNRRTL 1228

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ACXE	NP_652601.3	AC_N	<31..272	CDD:318454	57/286 (20%)
		Nucleotidyl_cyc_III	290..459	CDD:325147	86/170 (51%)
		Nucleotidyl_cyc_III	851..1071	CDD:325147	84/234 (36%)
Adcy8	NP_033753.2	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..180		12/55 (22%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..107
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	14..33
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	50..118
		AC_N	<159..398	CDD:292831	52/276 (19%)
		CYCc	364..562	CDD:214485	90/206 (44%)
		Guanylate_cyc	403..587	CDD:278633	92/185 (50%)
		DUF1053	615..709	CDD:283888	18/105 (17%)
		CYCc	942..1146	CDD:214485	82/240 (34%)
		Guanylate_cyc	971..1170	CDD:278633	85/235 (36%)
		Involved in CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1107..1249		48/124 (39%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000250\|UniProtKB:P40146	1198..1213		2/14 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1221..1249		2/8 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S5228
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.760