DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ACXE and ADCY8

DIOPT Version :9

Sequence 1:	NP_652601.3	Gene:	ACXE / 53426	FlyBaseID:	FBgn0040506	Length:	1123	Species:	Drosophila melanogaster
Sequence 2:	NP_001106.1	Gene:	ADCY8 / 114	HGNCID:	239	Length:	1251	Species:	Homo sapiens

Alignment Length:	1259	Identity:	342/1259 - (27%)
Similarity:	548/1259 - (43%)	Gaps:	311/1259 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 GYLKAKCAELRLESEFRL-----YR----------------IRLWKSYL---------------- 48
            |:|...||.  ..|:|.|     ||                .||::.|.
Human   128 GFLHLDCAP--SNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDV 190

  Fly    49 ---LTFFMLHIFVTSVHC----ALLLATIERRSIIYFDVALSIGCALVLILVLSVNFCDEFIAKH 106
               ||..:||:.:.|...    .:||.        :|.....:.||||::        .:....|
Human   191 LTKLTLLVLHLSLASAPMDPLKGILLG--------FFTGIEVVICALVVV--------RKDTTSH 239

  Fly   107 TWYMYASSIFASLTLVFADLTESIYHTYAHSWIL--GTFYDTYIIYMIYMFLPIHFISGAVLLAL 169
            |:..|:.      .:.:..:|..|........:|  |..|..:.::..|..||:. ::.|:|..|
Human   240 TYLQYSG------VVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLP-LTWAILAGL 297

  Fly   170 LVSGLYILYFVIFIAQGFAQFASALFSVGGMSVDIVHYLCLNLVGIFYRVMNDTVVRSSFLDRHQ 234
            ..|.|.::..|:..       ..|:.|:..:....|.::|:|..|||...::|...|.:||:..:
Human   298 GTSLLQVILQVVIP-------RLAVISINQVVAQAVLFMCMNTAGIFISYLSDRAQRQAFLETRR 355

  Fly   235 YIKEKIWLRNARLQEKQLLDSILPPQISLPLQKDIQGRIVMAKQGIHSWTAMERTMAIQIH--PD 297
            .::.::.|.....::::|:.|:||..:.|.:..|:        ..:.......:...|.||  .:
Human   356 CVEARLRLETENQRQERLVLSVLPRFVVLEMINDM--------TNVEDEHLQHQFHRIYIHRYEN 412

  Fly   298 VSILYADVVNYTHLTTTLTVEMLVKVLHDLYGRFDLAAYRYKVQRIKFLGDCYYCVAGLSDPDPD 362
            ||||:|||..:|:|:|||:.:.||::|::|:.|||..|:.:...|||.||||||||:||.:|..|
Human   413 VSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQD 477

  Fly   363 HANNCVILGLSMINHIMEVRDIHGLDINMRIGVHSGNLFAGVIGEAKLQFDIWGLDVTIANVLES 427
            ||:.||.:|||||..|..||.....|::||||:|||::..||:|..|.|||:|..||.|||.|||
Human   478 HAHCCVEMGLSMIKTIRYVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLES 542

  Fly   428 TGVPGCVHISGATLNNLDVNRFDIEDGPEEARDHPLLKKYRIRSYIIRQDLHMDDEDSDEFLGDL 492
            .|:||.:|||.|||:.|: ..:::|:|..:.|:. .|:|:.|.:|:|:|     .|||
Human   543 GGIPGRIHISKATLDCLN-GDYNVEEGHGKERNE-FLRKHNIETYLIKQ-----PEDS------- 593

  Fly   493 HSISLCNMGAQPRISDSANQSLRALFHEELREEFRKMPVSAFSPK-----------RLLGICRFN 546
             .:||.....:..:|.|..::..|.|.|           .::||:           .|..:.| |
Human   594 -LLSLPEDIVKESVSSSDRRNSGATFTE-----------GSWSPELPFDNIVGKQNTLAALTR-N 645

  Fly   547 TGKEVPAH--QNLNI-------------------------------CLTFTDPILERAYLKQTDY 578
            :...:|.|  |.|::                               .|.|.|..||..|.:..|.
Human   646 SINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDE 710

  Fly   579 MYKYSIILSASVGCS-LVYIELMDTQMICSSCFVLPASV-----ATIQCILALIAWYKKY----- 632
            ::|.:::      |: :|.:.:...|.:..|..|:|.::     ..:...|.||...:.|
Human   711 VFKSNLV------CAFIVLLFITAIQSLLPSSRVMPMTIQFSILIMLHSALVLITTAEDYKCLPL 769

  Fly   633 ------CW---TRYGRNNVPHHYNGFSCFIFRIHDKILN--------SLPIRICIYLFLMISSFF 680
                  ||   |...||.:.     |:..:......|||        |:|::
Human   770 ILRKTCCWINETYLARNVII-----FASILINFLGAILNILWCDFDKSIPLK------------- 816

  Fly   681 VMCLIVMSCQREEFEMAYIEERLFHYEQEAHICFHP--WVTTNMLSLMICLTFTFAHIPIMVKTA 743
                          .:.:....:|     ..||.:|  :|.|.:|:::.|.  .|..:..::|.|
Human   817 --------------NLTFNSSAVF-----TDICSYPEYFVFTGVLAMVTCA--VFLRLNSVLKLA 860

  Fly   744 VAILETLAYLLL-------IFFQFDFVFHHSVTTNPYFKSEYAHALLICITFLIMFVKERQIEFT 801
            |.::....|.||       :|.::|.:.|   :...:..::....||:.:..|.:|...:|:|:|
Human   861 VLLIMIAIYALLTETVYAGLFLRYDNLNH---SGEDFLGTKEVSLLLMAMFLLAVFYHGQQLEYT 922

  Fly   802 NKVNFNWRVDLRKEENAASLTNHSIIIILNNILPSHIVDVYL-NSLAKHELYFENYRMVSVMFAM 865
            .:::|.|||..::|.|...........:|.||||||:...:| ......|||.::|..|.||||.
Human   923 ARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARHFLEKDRDNEELYSQSYDAVGVMFAS 987

  Fly   866 LINF-------EMD---LRSLRVLNEIIAEFDTLLLFYKEYYTVEKIKIVGCTYMAACGLDLNFA 920
            :..|       ||:   :..||:||||||:||. ||....:..:||||.:|.||||..||...
Human   988 IPGFADFYSQTEMNNQGVECLRLLNEIIADFDE-LLGEDRFQDIEKIKTIGSTYMAVSGLSPE-- 1049

  Fly   921 GSTSTNRKESIPPTEFNEEQSRRILFQQSNEDLDEVVFVMTSYALDMMRTLAKSNEAYQSIAGDR 985
                                      :|..||....:..:..::|.:..::.:.|:        .
Human  1050 --------------------------KQQCEDKWGHLCALADFSLALTESIQEINK--------H 1080

  Fly   986 NITDGTIAIGISSGEVMAGIVGASQPHYDIWGNPVNMASRMESTGLPGHIHVTEETSEILQQFGI 1050
            :..:..:.||||.|.|:||::||.:|.|||||..||:||||:|||:.|.|.|.|||..||:..|.
Human  1081 SFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGF 1145

  Fly  1051 TCSYRGMTFVKG----RGKIPTY-LVG-IDENLNFIPQKATRFPSHQERSTVI-----SL----Q 1100
            ...|||..:|||    .|||.|| |:| :..|...:|.:  |.|.....:.|:     ||    |
Human  1146 AFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPR--RLPGQYSLAAVVLGLVQSLNRQRQ 1208

  Fly  1101 STYTHAENNNSI--ASTSRHTLQS 1122
            ....:..||..|  ...:|.||.|
Human  1209 KQLLNENNNTGIIKGHYNRRTLLS 1232

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ACXE	NP_652601.3	AC_N	<31..272	CDD:318454	56/286 (20%)
		Nucleotidyl_cyc_III	290..459	CDD:325147	86/170 (51%)
		Nucleotidyl_cyc_III	851..1071	CDD:325147	84/234 (36%)
ADCY8	NP_001106.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..182		12/55 (22%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..109
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	50..92
		AC_N	<161..400	CDD:292831	51/276 (18%)
		CYCc	366..564	CDD:214485	90/206 (44%)
		Guanylate_cyc	405..589	CDD:278633	92/185 (50%)
		DUF1053	617..711	CDD:283888	18/105 (17%)
		CYCc	944..1148	CDD:214485	82/240 (34%)
		Guanylate_cyc	973..1172	CDD:278633	85/235 (36%)
		Involved in CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1109..1251		50/126 (40%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000250\|UniProtKB:P40146	1200..1215		3/14 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1223..1251		4/10 (40%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S5228
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.760