Protein Alignment PIK3CG and VPS34

DIOPT Version :10

• Sequence 1: NP_002640.2
• Gene: PIK3CG / 5294
• HGNCID: 8978
• Length: 1102
• Species: Homo sapiens
• Sequence 2: NP_013341.1
• Gene: VPS34 / 850941
• SGDID: S000004230
• Length: 875
• Species: Saccharomyces cerevisiae
• Alignment Length: 903
• Identity: 231/903 - (25%)
• Similarity: 367/903 - (40%)
• Gaps: 239/903 - (26%)

Human   350 FTVSLWDCDRKFRVKIRGID--IPVLP-----RNTDL-----TVFVEANIQHGQQVLCQRR---- 398
            |.|| .|.|...:|||:.::  .|:|.     .|.:|     .||..:::....||..:.|
Yeast     8 FCVS-QDLDVPLKVKIKSLEGHKPLLKPSQKILNPELMLIGSNVFPSSDLIVSLQVFDKERNRNL 71

Human   399 -----TSPKPFTEEVLWNVWLEFSIKIKDLPKGALLNLQIYCGKAPALSSKASAESPSSESKGKV 458
                 |...||.....|:.||...|:||.|...:.|.:.::                  |..|..
Yeast    72 TLPIYTPYIPFRNSRTWDYWLTLPIRIKQLTFSSHLRIILW------------------EYNGSK 118

Human   459 QLLYY------VNLL----------------LIDHRFLLR------------RGEYVLHMW---- 485
            |:.::      .||.                :|||..::.            :||:....|
Yeast   119 QIPFFNLETSIFNLKDCTLKRGFESLKFRYDVIDHCEVVTDNKDQENLNKYFQGEFTRLPWLDEI 183

Human   486 -------QISGKGEDQGSF--------------------------------NADKLTSATNPDKE 511
                   |...:...||:|                                |....|....|::
Yeast   184 TISKLRKQRENRTWPQGTFVLNLEFPMLELPVVFIEREIMNTQMNIPTLKNNPGLSTDLREPNR- 247

Human   512 NSMSISILLDNYCHPIA--LPKHQPTPDPEGDRVR--------AEMPNQL------RKQLEAIIA 560
            |...|.|.|.:..|...  ....||..||..::.|        |.:..|:      |..|..||.
Yeast   248 NDPQIKISLGDKYHSTLKFYDPDQPNNDPIEEKYRRLERASKNANLDKQVKPDIKKRDYLNKIIN 312

Human   561 TDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQQEIVAKTYQLLARREVWDQSALDVG 625
            ..|...|||.:|..:|.:||..:.:.||..||..|....::   ::..::|...:.|  :.:|:.
Yeast   313 YPPGTKLTAHEKGSIWKYRYYLMNNKKALTKLLQSTNLREE---SERVEVLELMDSW--AEIDID 372

Human   626 LTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFE-------------------- 670
            ..::||...|.:.:||:.||.:|:...|.::..||||||:||.||
Yeast   373 DALELLGSTFKNLSVRSYAVNRLKKASDKELELYLLQLVEAVCFENLSTFSDKSNSEFTIVDAVS 437

Human   671 ------------------------------------PYHDSALARFLLKRGLRNKRIGHFLFWFL 699
                                                |...|.||.||::|.|.|.|:|.|.:|:|
Yeast   438 SQKLSGDSMLLSTSHANQKLLKSISSESETSGTESLPIVISPLAEFLIRRALVNPRLGSFFYWYL 502

Human   700 RSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIEMLQKVTLDIKSL---SAEKYDVSS 761
            :||.....:..|    ||.::.........:....||::|.:|::....||.|   :|:|.::
Yeast   503 KSESEDKPYLDQ----ILSSFWSRLDKKSRNILNDQVRLINVLRECCETIKRLKDTTAKKMEL-- 561

Human   762 QVISQLKQKLENLQNSQLPESFRVPYDPGLKAGALAIEKCKVMASKKKPLWLEFKCADPTALSNE 826
             ::..|:.|:..|...:   ...:|.||.:....:..|..||..|...||.:.||    |.| |:
Yeast   562 -LVHLLETKVRPLVKVR---PIALPLDPDVLICDVCPETSKVFKSSLSPLKITFK----TTL-NQ 617

Human   827 TIGIIFKHGDDLRQDMLILQILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKI 891
            ...::||.|||||||.|::||:.:|..:.:.|::||.|.||..::||.:.|.||.:.: .|:|.|
Yeast   618 PYHLMFKVGDDLRQDQLVVQIISLMNELLKNENVDLKLTPYKILATGPQEGAIEFIPN-DTLASI 681

Human   892 QQSTVGNTGAFKDEVLNHWLKEKSPTEEKFQA----AVERFVYSCAGYCVATFVLGIGDRHNDNI 952
            .....|..|         :||...|.|.....    .::.||.|||||||.|::||:||||.||:
Yeast   682 LSKYHGILG---------YLKLHYPDENATLGVQGWVLDNFVKSCAGYCVITYILGVGDRHLDNL 737

Human   953 MITETGNLFHIDFGHILG-NYKSFLGINKERVPFVLTPDFLFVMGTSGKKTSPHFQKFQDICVKA 1016
            ::|..|:.||.|||:||| :.|.|..:.|      |.|..:...|  |.::| ::.||:..|..|
Yeast   738 LVTPDGHFFHADFGYILGQDPKPFPPLMK------LPPQIIEAFG--GAESS-NYDKFRSYCFVA 793

Human  1017 YLALRHHTNLLIILFSMMLMTGMPQLTSKED--IEYIRDALTVGKNEEDAKKYFLDQI 1072
            |..||.:..|::.||.:|..:.:|.:....:  |..:|:...:..:||||..:|.:.|
Yeast   794 YSILRRNAGLILNLFELMKTSNIPDIRIDPNGAILRVRERFNLNMSEEDATVHFQNLI 851

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PIK3CG	NP_002640.2	PIK3CG_ABD	1..192	CDD:466155
		PI3K_rbd	203..312	CDD:197540
		C2_PI3K_class_I_gamma	350..527	CDD:176044	51/274 (19%)
		PI3Ka_I	549..725	CDD:238444	59/237 (25%)
		PI3Kc_IB_gamma	729..1095	CDD:270627	115/354 (32%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	803..809		2/5 (40%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	943..951		5/7 (71%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	962..988		10/26 (38%)
VPS34	NP_013341.1	TEL1	<1..872	CDD:227365	231/903 (26%)
		PI3Kc_III	528..871	CDD:270628	115/354 (32%)