Protein Alignment PIK3CB and Pi3K92E

DIOPT Version :10

• Sequence 1: NP_006210.1
• Gene: PIK3CB / 5291
• HGNCID: 8976
• Length: 1070
• Species: Homo sapiens
• Sequence 2: NP_650902.1
• Gene: Pi3K92E / 42446
• FlyBaseID: FBgn0015279
• Length: 1088
• Species: Drosophila melanogaster
• Alignment Length: 1086
• Identity: 412/1086 - (37%)
• Similarity: 625/1086 - (57%)
• Gaps: 53/1086 - (4%)

Human     8 PPAMADILDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFNLL 72
            ||.|...:::|..:..    ..:.:..|||.|..::|.|....||..||..:..|....|:..::
  Fly    27 PPCMRFSVNLWKNEML----NWVDLICLLPNGFLLELRVNPANTIQVIKVEMVNQAKQMPLGYVI 87

Human    73 MDIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKL----VTRSCDPGEKLDSKIGVLIGKG 133
            .:...|....:: |...|...|||:||.:|:|:..:|.|    .|.|.....:|...:..:||..
  Fly    88 KEACEYQVYGIS-TFNIEPYTDETKRLSEVQPYFGILSLGERTDTTSFSSDYELTKMVNGMIGTT 151

Human   134 LHEFDSLKDPEVNEFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPE--HEPSIPENLEDKLYGGK 196
            .....:...||:::||..|.:..:...|.....:|...|...:|..  :...:||.:.::.....
  Fly   152 FDHNRTHGSPEIDDFRLYMTQTCDNIELERSAYTWQQRLLYEHPLRLANSTKMPELIRERHPTRT 216

Human   197 LIVAVHFENCQDVFSFQVSPNMNPIKVNELAIQKRLTIHGK-EDEVSPYDYVLQVSGRVEYVFGD 260
            .::.|..||.|..|:..|:....|..:.|..:||......| .|..|  ||:|:||||.||:.||
  Fly   217 FLIVVKNENDQSTFTLSVNEQDTPFSLTESTLQKMNRSQMKMNDRTS--DYILKVSGRDEYLLGD 279

Human   261 HPLIQFQYIRNCVMNRALPHFILVECCKIKKMYEQEMIAIEAAINRNSSNLPL---PLPPKKT-- 320
            :|||||.||:..:.:.|:|:.:|           |.:..:|:.||.::....:   |||.|:|
  Fly   280 YPLIQFLYIQEMLSDSAVPNVVL-----------QSVYRLESYINHHNEQAMVTKRPLPKKRTVH 333

Human   321 --RIISHVWENNNPFQIVLVKGNKLNTEET--VKVHVRAGLFHGTELLCKTIVSSEVSGKND-HI 380
              :.||.:|:..|.||:.|...:.:|.::|  :||.|...|:||.:.||....:...:|..| .:
  Fly   334 LHKSISSLWDMGNYFQLTLHSISNVNFDKTRALKVGVHVCLYHGDKKLCAQRSTDSPNGNFDTFL 398

Human   381 WNE-PLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTINPSKYQTIRKAGKVHYPVAWVNTM 444
            :|: .::|||.:.:||||.|||..::.|....::|||:.    :|...::.......|:|||||.
  Fly   399 FNDLVMDFDIQMRNLPRMTRLCIVIFEVTKMSRSKKSSN----NKDIALKDVPYNKNPLAWVNTT 459

Human   445 VFDFKGQLRTGDIILHSWSSFPDELE--EMLNPMGTVQTNPYTENATALHVKFPENKKQPYYYPP 507
            :||.|..||||...|::| ::.|:::  |:.:|:||::.||..|....:.:.|..:......||.
  Fly   460 IFDHKDILRTGRHTLYTW-TYADDIQSVEVFHPLGTIEPNPRKEECALVDLTFLSSGTGTVRYPS 523

Human   508 FDKIIEKAAEIASSDSANVSSRGGKKFLPVLKEILDR----DPLSQLCENEMDLIWTLRQDCREI 568
            .:.:::.||:....:.......|.:|.:..|||::..    |.:.::.:.:.:.||..|.|....
  Fly   524 EEVVLQYAADREQVNRLQRQLAGPEKPIKELKELMANYTGLDKIYEMVDQDRNAIWERRNDILRE 588

Human   569 FPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQMSDE 633
            .|:.|..||..:.|.:.:|||.:..||:.||.:....:|||||:.|||..||.:|:.||..:.||
  Fly   589 LPEELSILLHCVYWKERDDVADMWYLLKQWPLISIERSLELLDYAYPDPAVRRFAIRCLHFLKDE 653

Human   634 ELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRIGQFLFWHLRSEVHIPAVSVQFGVILEAY 698
            :|..|||||||.:|:|.:|:..|..|||||||.|:|||.:.|||||||:..|::..:||::||.|
  Fly   654 DLLLYLLQLVQAIKHESYLESDLVVFLLERALRNQRIGHYFFWHLRSEMQTPSMQTRFGLLLEVY 718

Human   699 CRGSVGHMKVLSKQVEALNKLKTLNSLIKLNAVKLNRAKGKEAMHTCLKQSAYREALSDLQSPLN 763
            .:|...|:..|.||:..|.|||. .|||   |.|.::.|.|..:...|:.........::|:|||
  Fly   719 LKGCKHHVAPLRKQLHVLEKLKQ-GSLI---AKKGSKEKVKTMLQDFLRDQRNSAVFQNIQNPLN 779

Human   764 PCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWK 828
            |....|.:..::||.|||||:|||:|:.|.......|.:|||||||||||||||||||:||.|||
  Fly   780 PSFRCSGVTPDRCKVMDSKMRPLWVVFENADVNASDVHIIFKNGDDLRQDMLTLQMLRVMDQLWK 844

Human   829 EAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNS-GD 892
            ..|:|.||..|.|::.....|:||||..:||||:||......:|.:.|.|.:||:||||:|. .|
  Fly   845 RDGMDFRMNIYNCISMEKSLGMIEVVRHAETIANIQKEKGMFSATSPFKKGSLLSWLKEHNKPAD 909

Human   893 DLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVP 957
            .|::||.||||||||||||:||||:.||||||||||:.|||||||||||||:||.|.|::|||||
  Fly   910 KLNKAINEFTLSCAGYCVATYVLGVADRHSDNIMVKRNGQLFHIDFGHILGHFKEKLGVRRERVP 974

Human   958 FILTYDFIHVIQQG-KTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDI 1021
            |:||:||::||.:| ....:::|..|::.||.|:|:||:||.|.::||::|::.|||||:|.||:
  Fly   975 FVLTHDFVYVINKGFNDRESKEFCHFQELCERAFLVLRKHGCLILSLFSMMISTGLPELSSEKDL 1039

Human  1022 QYLKDSLALGKSEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKD 1067
            .||:::|.|..:||:|.:.|:.||.|||..||.|.:||.:|...|:
  Fly  1040 DYLRETLVLDYTEEKAREHFRAKFSEALANSWKTSLNWASHNFSKN 1085

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PIK3CB	NP_006210.1	PI3K_p85B	41..118	CDD:197539	22/80 (28%)
		PI3K_rbd	180..288	CDD:197540	36/108 (33%)
		C2_PI3K_class_I_beta_delta	324..501	CDD:176075	58/182 (32%)
		Nuclear localization signal	410..418		3/7 (43%)
		PI3Ka_I	532..702	CDD:238444	72/173 (42%)
		PI3Kc_IA_beta	706..1067	CDD:270717	186/362 (51%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	778..784		4/5 (80%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	916..924		5/7 (71%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	935..961		19/25 (76%)
Pi3K92E	NP_650902.1	PI3K_p85B	56..132	CDD:470589	20/76 (26%)
		PI3K_rbd	200..307	CDD:197540	37/119 (31%)
		C2_PI3K_class_I_beta_delta	339..517	CDD:176075	58/182 (32%)
		PI3Ka_I	552..723	CDD:238444	72/170 (42%)
		PI3Kc_I	727..1084	CDD:270709	186/360 (52%)