Protein Alignment PIK3CB and age-1

DIOPT Version :10

• Sequence 1: NP_006210.1
• Gene: PIK3CB / 5291
• HGNCID: 8976
• Length: 1070
• Species: Homo sapiens
• Sequence 2: NP_496462.2
• Gene: age-1 / 174762
• WormBaseID: WBGene00000090
• Length: 1182
• Species: Caenorhabditis elegans
• Alignment Length: 1196
• Identity: 318/1196 - (26%)
• Similarity: 550/1196 - (45%)
• Gaps: 192/1196 - (16%)

Human     6 IMPPAMADILDIWAVDSQ-------------IASDGSIPVDFLLPTGIYIQLEVP---------R 48
            |:.|.:..:::.|.:..:             :.::|...:..:.|.|..|.:..|         .
 Worm    41 ILHPQLQTMVEQWQMRERPSLETENGKGSLLLENEGVADIITMCPFGEVISVVFPWFLANVRTSL 105

Human    49 EATISYIKQMLWKQVHNYPMFNLLMDIDSYMFACVNQTAVYEEL--EDETRRLCDVRPFLPVLKL 111
            |..:|..|..|::.:.........:....|:|..:|.....|.:  :|:.....::....|:|.|
 Worm   106 EIKLSDFKHQLFELIAPMKWGTYSVKPQDYVFRQLNNFGEIEVIFNDDQPLSKLELHGTFPMLFL 170

Human   112 -----VTRSCDPGEKLDSKIGVLIGKGLHEFDSLKDPEVNEFRRKMRKFSEEKILS--LVGLSWM 169
                 :.|.    ::|.|.|...:|..|.:.:...|.|:.:||..:...:::..|:  |.|.|..
 Worm   171 YQPDGINRD----KELMSDISHCLGYSLDKLEESLDEELRQFRASLWARTKKTCLTRGLEGTSHY 231

Human   170 DWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFEN--------CQDVFSFQV--SPNMNPIKV- 223
            .:.::.|....| |.|::||.|:...||...:.:..        |:.:...|:  :||..|..:
 Worm   232 AFPEEQYLCVGE-SCPKDLESKVKAAKLSYQMFWRKRKAEINGVCEKMMKIQIEFNPNETPKSLL 295

Human   224 -NELAIQKRLTIHGKEDEVSPYD--YVLQVSGRVEYVFG-DHPLIQFQYIRNCVMNRALPHF--- 281
             ..|...::|.::..:|   |.|  :.||::||..:|.. |..|..:..:|:.:.:...|.|
 Worm   296 HTFLYEMRKLDVYDTDD---PADEGWFLQLAGRTTFVTNPDVKLTSYDGVRSELESYRCPGFVVR 357

Human   282 ----ILVECCKIKKMYEQEMI-AIEAAINRNSSNLPLPLPPKKTRIISHV--------------- 326
                :|.:.|:.|.:||...: |.|..:..:..::.:...||:::....|
 Worm   358 RQSLVLKDYCRPKPLYEPHYVRAHERKLALDVLSVSIDSTPKQSKNSDMVMTDFRPTASLKQVSL 422

Human   327 WENNNPFQI--VLVKGNKLNTEETVKVHVRAGLFHGTELLCKTIVSSEVSGKNDHI--WN-EPLE 386
            |:.:....|  |.:.|.....:..:.|.:...::.||    .|:.|...:..|...  || |...
 Worm   423 WDLDANLMIRPVNISGFDFPADVDMYVRIEFSVYVGT----LTLASKSTTKVNAQFAKWNKEMYT 483

Human   387 FDINICDLPRMARLCFAVYAVLDKVKTKKSTKTINPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQ 451
            ||:.:.|:|..|.|  ::..:..|||.|..                  .:.|.|||..:.|::.:
 Worm   484 FDLYMKDMPPSAVL--SIRVLYGKVKLKSE------------------EFEVGWVNMSLTDWRDE 528

Human   452 LRTGDIILHSWSSFPDELEEMLNPMG---------TVQTNPYTENATALHVKFPENKKQPYYYPP 507
            ||.|..:.|.|:..|......:...|         |::.:.|     ...|:.|...:..|....
 Worm   529 LRQGQFLFHLWAPEPTANRSRIGENGARIGTNAAVTIEISSY-----GGRVRMPSQGQYTYLVKH 588

Human   508 FDKIIEKAAEIASSDSANVSSRGGKKFLPVLKE-----ILDRDPLSQLCENEMDLIWTLRQDCRE 567
            .....|....:.....:.:...|.||...::|:     :|:        |:|...:|..|:..::
 Worm   589 RSTWTETLNIMGDDYESCIRDPGYKKLQMLVKKHESGIVLE--------EDEQRHVWMWRRYIQK 645

Human   568 IFPQSLPKLL-LSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCL-RQM 630
            ..|..|..|. |:..|...|:.::|..:|:.|.......||.||.....|:.:|::||..| .|:
 Worm   646 QEPDLLIVLSELAFVWTDRENFSELYVMLEKWKPPSVAAALTLLGKRCTDRVIRKFAVEKLNEQL 710

Human   631 SDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRIGQFLFWHLRSEV----HIPAVSVQF 691
            |......::|.|:|.|||||.....:...||.|||.:.|||..|||.||:|:    .....|.::
 Worm   711 SPVTFHLFILPLIQALKYEPRAQSEVGMMLLTRALCDYRIGHRLFWLLRAEIARLRDCDLKSEEY 775

Human   692 ---GVILEAYCRGSVGHMKVLSKQVEALNKLKTLNSLIK-----LNAVKLNRAKGKEAMHTCLKQ 748
               .:::|||.||:..|:|::::||:.:::|..:::|:|     :..:|| |.:.:...|
 Worm   776 RRISLLMEAYLRGNEEHIKIITRQVDMVDELTRISTLVKGMPKDVATMKL-RDELRSISH----- 834

Human   749 SAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGED----SVGVIFKNGDD 809
                 .:.::.|||:|...|.|:.::|...:.|..:||.|.:.||....|    ...:|||||||
 Worm   835 -----KMENMDSPLDPVYKLGEMIIDKAIVLGSAKRPLMLHWKNKNPKSDLHLPFCAMIFKNGDD 894

Human   810 LRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAA 874
            ||||||.||:|.:||.:||.|.:|..:.||..|..|:..|:||||...:||.:||:.:..:..|.
 Worm   895 LRQDMLVLQVLEVMDNIWKAANIDCCLNPYAVLPMGEMIGIIEVVPNCKTIFEIQVGTGFMNTAV 959

Human   875 AFNKDALLN-WLKEYNSGDD-----------------LD---------RAIEEFTLSCAGYCVAS 912
            .....:.:| |:::....:|                 :|         .:::.|..||.||.||:
 Worm   960 RSIDPSFMNKWIRKQCGIEDEKKKSKKDSTKNPIEKKIDNTQAMKKYFESVDRFLYSCVGYSVAT 1024

Human   913 YVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKT--GN 975
            |::||.||||||:|:.:.|:.||||||||||:.|:|.||:|:|.|||||..|:.||:.||:  ||
 Worm  1025 YIMGIKDRHSDNLMLTEDGKYFHIDFGHILGHGKTKLGIQRDRQPFILTEHFMTVIRSGKSVDGN 1089

Human   976 TEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSL-ALGKSEEEALK 1039
            :.:..:|:..|.:||.::..:.:||::||.|||...||||::..|:.:||.:| ..|:|:|||.|
 Worm  1090 SHELQKFKTLCVEAYEVMWNNRDLFVSLFTLMLGMELPELSTKADLDHLKKTLFCNGESKEEARK 1154

Human  1040 QFKQKFDEALRESWTTKVNWMAHTVR 1065
            .|...::||...||:||.||:.|.|:
 Worm  1155 FFAGIYEEAFNGSWSTKTNWLFHAVK 1180

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PIK3CB	NP_006210.1	PI3K_p85B	41..118	CDD:197539	15/92 (16%)
		PI3K_rbd	180..288	CDD:197540	29/129 (22%)
		C2_PI3K_class_I_beta_delta	324..501	CDD:176075	40/205 (20%)
		Nuclear localization signal	410..418		4/7 (57%)
		PI3Ka_I	532..702	CDD:238444	55/183 (30%)
		PI3Kc_IA_beta	706..1067	CDD:270717	146/399 (37%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	778..784		1/5 (20%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	916..924		6/7 (86%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	935..961		18/25 (72%)
age-1	NP_496462.2	PI3K_p85B	98..177	CDD:197539	12/78 (15%)
		PI3K_rbd	241..360	CDD:197540	28/122 (23%)
		C2_PI3K_like	420..574	CDD:176026	37/182 (20%)
		PI3Ka	606..798	CDD:214537	59/199 (30%)
		Protein Kinases, catalytic domain	793..1181	CDD:473864	146/399 (37%)