Protein Alignment PIK3CA and VPS34

DIOPT Version :10

• Sequence 1: NP_006209.2
• Gene: PIK3CA / 5290
• HGNCID: 8975
• Length: 1068
• Species: Homo sapiens
• Sequence 2: NP_013341.1
• Gene: VPS34 / 850941
• SGDID: S000004230
• Length: 875
• Species: Saccharomyces cerevisiae
• Alignment Length: 846
• Identity: 217/846 - (25%)
• Similarity: 359/846 - (42%)
• Gaps: 199/846 - (23%)

Human   371 VNTQRVPCSNPR-WNEWLNYDIYIPDLPRAARLCLSICSVKGRK------------GAKE----- 417
            :.|..:|..|.| |:.||...|.|..|..::.|.:.:....|.|            ..|:
Yeast    75 IYTPYIPFRNSRTWDYWLTLPIRIKQLTFSSHLRIILWEYNGSKQIPFFNLETSIFNLKDCTLKR 139

Human   418 ------------EHCPLAWGN---INLFDY------------------------TDTLVSGKMAL 443
                        :||.:...|   .||..|                        ..|...|...|
Yeast   140 GFESLKFRYDVIDHCEVVTDNKDQENLNKYFQGEFTRLPWLDEITISKLRKQRENRTWPQGTFVL 204

Human   444 NL------WPVPHGLEDLLNP---IGVTGSN---------PNKETPCLELEF-DWFSSVVKF--- 486
            ||      .||.....:::|.   |....:|         ||:..|.:::.. |.:.|.:||
Yeast   205 NLEFPMLELPVVFIEREIMNTQMNIPTLKNNPGLSTDLREPNRNDPQIKISLGDKYHSTLKFYDP 269

Human   487 --PDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRD---------PL 540
              |:...|||            .|      .||.|.::....||:....|..||         |.
Yeast   270 DQPNNDPIEE------------KY------RRLERASKNANLDKQVKPDIKKRDYLNKIINYPPG 316

Human   541 SEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCN 605
            :::|..||..:|.:|:|.:...:.|.|||.|.......|..::..|:..|..|..:.|:|||...
Yeast   317 TKLTAHEKGSIWKYRYYLMNNKKALTKLLQSTNLREESERVEVLELMDSWAEIDIDDALELLGST 381

Human   606 YPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYE---------------------QYL--DN 647
            :.:..||.:||..|:| .:|.:|..||:|||:.:.:|                     |.|  |:
Yeast   382 FKNLSVRSYAVNRLKK-ASDKELELYLLQLVEAVCFENLSTFSDKSNSEFTIVDAVSSQKLSGDS 445

Human   648 LLV---------------------------------RFLLKKALTNQRIGHFFFWHLKSEMHNKT 679
            :|:                                 .||:::||.|.|:|.||:|:||||..:|.
Yeast   446 MLLSTSHANQKLLKSISSESETSGTESLPIVISPLAEFLIRRALVNPRLGSFFYWYLKSESEDKP 510

Human   680 VSQRFGLLLESYCRACGMYLKH-LNRQVEAMEKLINLTDILKQEKKDETQKVQ-MKFLVEQMRRP 742
            ...:   :|.|:........:: ||.||..:..|....:.:|:.|....:|:: :..|:|...||
Yeast   511 YLDQ---ILSSFWSRLDKKSRNILNDQVRLINVLRECCETIKRLKDTTAKKMELLVHLLETKVRP 572

Human   743 DFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDL 807
              :..::....||:|...:.::..|..::..|:..||.:.::.      .|.|...::||.||||
Yeast   573 --LVKVRPIALPLDPDVLICDVCPETSKVFKSSLSPLKITFKT------TLNQPYHLMFKVGDDL 629

Human   808 RQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCK-GGLKGALQ 871
            |||.|.:|||.:|..:.:|:.:||::.||..|:.|...|.||.:.|. |:..|..| .|:.|.|:
Yeast   630 RQDQLVVQIISLMNELLKNENVDLKLTPYKILATGPQEGAIEFIPND-TLASILSKYHGILGYLK 693

Human   872 FNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGH 936
                 ||...::...| :....:|.|.:|||||||.|:|||:||||..|::|..||..||.|||:
Yeast   694 -----LHYPDENATLG-VQGWVLDNFVKSCAGYCVITYILGVGDRHLDNLLVTPDGHFFHADFGY 752

Human   937 FLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINL 1001
            .|....|.|      .|.:.....:|....||:    :..:::|:..|:.||..:|::|.|.:||
Yeast   753 ILGQDPKPF------PPLMKLPPQIIEAFGGAE----SSNYDKFRSYCFVAYSILRRNAGLILNL 807

Human  1002 FSMMLGSGMPELQSFDD--IAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQ 1064
            |.:|..|.:|:::...:  |..:|:...|:.:|::|..:|...:||:.:......:|.: |.:.|
Yeast   808 FELMKTSNIPDIRIDPNGAILRVRERFNLNMSEEDATVHFQNLINDSVNALLPIVIDHL-HNLAQ 871

Human  1065 H 1065
            :
Yeast   872 Y 872

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PIK3CA	NP_006209.2	PI3K_p85B	31..108	CDD:197539
		PI3K_rbd	173..292	CDD:197540
		C2_PI3K_class_I_alpha	325..484	CDD:176043	35/188 (19%)
		PI3Ka_I	525..696	CDD:238444	60/235 (26%)
		PI3Kc_IA_alpha	695..1064	CDD:270719	111/373 (30%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	771..777		1/5 (20%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	912..920		5/7 (71%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	931..957		8/25 (32%)
VPS34	NP_013341.1	TEL1	<1..872	CDD:227365	216/844 (26%)
		PI3Kc_III	528..871	CDD:270628	111/368 (30%)