Protein Alignment NLK and CG8565

DIOPT Version :10

• Sequence 1: NP_057315.3
• Gene: NLK / 51701
• HGNCID: 29858
• Length: 527
• Species: Homo sapiens
• Sequence 2: NP_573080.1
• Gene: CG8565 / 32538
• FlyBaseID: FBgn0030697
• Length: 790
• Species: Drosophila melanogaster
• Alignment Length: 635
• Identity: 114/635 - (17%)
• Similarity: 179/635 - (28%)
• Gaps: 309/635 - (48%)

Human   130 HHPQQQLDIEPDR-----PIGYGAFGVVWSVTDPRDGKRVALK--KMPNVFQNL-VSCKRVFREL 186
            :||....|:..:|     .:|:|.|..||...|.:..:..|:|  |...|::.. :....:|.::
  Fly   190 YHPVAIGDVFVNRYHVFKKLGWGHFSTVWLCYDTQMDRYCAVKVSKSAQVYKETGIDEIMLFSQM 254

Human   187 KMLCFFKHD------NVLSALDILQ--PPHIDYFEEIYVVTELMQSDLHKII----VSPQPLSSD 239
            .:     ||      :|:...|..:  .||   ...|.:|.|::..:|.|:|    ....|:|
  Fly   255 SL-----HDQHKYRSHVVGFYDFFEITGPH---GRHICLVLEVLGDNLLKVIERCFYKGMPIS-- 309

Human   240 HVKVFLYQILRGLKYLH-SAGILHRDIKPGNLLVNSNCV-------------LKI---------- 280
            ::|....|:|.|||:|| ..||:|.|:||.|:|:.||.|             ||.
  Fly   310 NIKQIAQQVLTGLKFLHEECGIIHTDLKPENVLLASNEVSVRTEIKTAIEVYLKANEGKLSPSSK 374

Human   281 ----------------------------------------------------------------- 280
                                                                             
  Fly   375 MTKTAKRRMQAKSKKVISFFKNHRRMLRRQGIEDLLYLAERGLIEPITAAMAVSDNLPFIPFGFD 439

Human   281 ---------------------------CD--------------FGLARVEELDES---------- 294
                                       ||              :.|..::.|||.
  Fly   440 GFMMMSDADCRTVQNSKLPEMEGMEYKCDRINLCLKSPELFLRYVLDIIKNLDEKELSTQDKRRR 504

Human   295 ----------------------------------------------------------------- 294
                                                                             
  Fly   505 FTQASGRGQTKKNARSRLRLASNAAYTTGTGWRSNRKINLNDIYPIDPANNECDVRVKIADLGNA 569

Human   295 ----RHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQLDLITD 355
                .|.|.::.|:.|||.|:::|: .|....|||||.|:..||.....||...|.
  Fly   570 CYFHHHFTDDIQTKEYRALEVILGA-GYCETADIWSVACLLWELATGTYLFDTHSK--------- 624

Human   356 LLGTPSLEAMRTA-----CEGAKAHILR-GPHKQ------------PSLPVL---------YTLS 393
             .|..:|:.:..|     |.....:::| |.|.:            .:|..|         |...
  Fly   625 -RGKYNLDEVHIAKIVETCGRIPWYLIRKGKHSRNFINSAGKLCNIETLKPLKLANILIRWYGWR 688

Human   394 SQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPV 458
            ::.:.|.|:.|..||..:|..||||..||...||           |.                 :
  Fly   689 TRQSTEFVNFLMPMLQTNPLSRISASKALESHYL-----------CN-----------------I 725

Human   459 TNPKFD-DTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKS 507
            ..|..| |::  ...|||::|:..|. ..|:........|.:..|:.|.|
  Fly   726 ALPGIDIDSY--YYYSVREIKDCTHT-KSERNSDEDSSSCSSIDSSEFSS 772

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NLK	NP_057315.3	Required for interaction with TAB2. /evidence=ECO:0000250|UniProtKB:O54949	1..304		58/402 (14%)
		Sufficient for interaction with DAPK3. /evidence=ECO:0000269|PubMed:21454679	1..125
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	22..72
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	90..140		3/9 (33%)
		Sufficient for interaction with DAPK3. /evidence=ECO:0000269|PubMed:21454679	124..416		92/541 (17%)
		STKc_NLK	137..508	CDD:173748	112/628 (18%)
		TQE	298..300		1/1 (100%)
		Required for homodimerization and kinase activation and localization to the nucleus. /evidence=ECO:0000250|UniProtKB:O54949	428..527		14/81 (17%)
		Required for interaction with TAB2. /evidence=ECO:0000250|UniProtKB:O54949	434..527		14/75 (19%)
CG8565	NP_573080.1	Protein Kinases, catalytic domain	192..722	CDD:473864	97/550 (18%)