Protein Alignment HOOK1 and Girdin

DIOPT Version :10

• Sequence 1: NP_056972.1
• Gene: HOOK1 / 51361
• HGNCID: 19884
• Length: 728
• Species: Homo sapiens
• Sequence 2: NP_647780.1
• Gene: Girdin / 38385
• FlyBaseID: FBgn0283724
• Length: 1381
• Species: Drosophila melanogaster
• Alignment Length: 732
• Identity: 175/732 - (23%)
• Similarity: 334/732 - (45%)
• Gaps: 105/732 - (14%)

Human    17 SLMIWLQTFNTASPCQDVKQLTSGVAMAQVLHQIDAAWF--------NESWLSRIKEDVGDNWRI 73
            |::.||::      |....:|.:|.......|.|.:.|.        |.|.|:.:.   |.:..|
  Fly    19 SIISWLES------CLPRAELLAGYTSLLDGHIIHSVWLQIDPEPQNNPSELNDLN---GKSLSI 74

Human    74 -KASNVKKVLQGIMSYYHEFLGQQISEALIPD---LNQITECSDPVE-LGRLLQLILGCAINCEK 133
             :|.|.:.|::.:.|::.|.|||.|  .::||   |....|..:.:| :..||.|:||.|:.|..
  Fly    75 ARAKNFECVVRNLKSFFEEELGQTI--LVLPDAFTLGHHPESKNGLEQMKTLLTLLLGAAVQCPN 137

Human   134 KQEHIQNIMTLEESVQHVVMTAIQELMSKEILSSPPNDAVGELEQQ-----LKRALEELQEALAE 193
            |:..|..|..|:...||.::..|:::.....|.. ..|::..|..|     :.|..:|......:
  Fly   138 KELFIARIKELDLETQHAIVGLIKQVTDSHSLVL-TEDSLERLTPQSMYTHILRLTKERDVMYLK 201

Human   194 KEELRQRCEELDMQVTTLQDEKNSLVSENEMMNEKLDQLDG-SFDDPNTVVAKKYFHAQLQLEQL 257
            ..:|  .|.|.:|..:      ::||...:.::......:| :...|::....:..|..::...|
  Fly   202 WIDL--ACVETEMTAS------DNLVECGQGVSVTRSPSNGTATSTPSSSSNSESNHLAVECADL 258

Human   258 QEENFRLEAAKDDYRVHCEELEKQLIEFQHRNDELTSLAEE-------TRALKDEIDVLRATSDK 315
            :.:|.:|....::...:..||.::|.:.:.|.|:|...::|       ..|.:||:|:||..:::
  Fly   259 RSKNRKLRQELEEKSENLLELREELDDKKARFDKLRQESQEWFTEAKRASAYRDEVDILRERAER 323

Human   316 ANKLESTVEIYRQKLQDLNDLRKQVKTLQETNMMYMHNTVSLEEELKK-------ANAARTQLET 373
            |::||..|:.||:||.|.:..:.:|:.|:|.|.:.:.:...|||:|::       |.:..:::..
  Fly   324 ADRLEVEVQKYREKLGDSDFYKSRVEELREDNRVLLESKEMLEEQLQRYRKRSEHAISLESEIIK 388

Human   374 YKRQVQDLHVKLSSESKRADTLAFEMKRLEEKHEALLKEKERLIEQRDTLKETNEELRCSQVQQD 438
            ||:::.|              :|.|......|.|.||:|..:|......|..|.:..:.....:|
  Fly   389 YKQKIND--------------MALERDVDRSKLEELLEENSQLQLVARNLNSTMDLDKSFSENED 439

Human   439 HLNQTDASATKSYENLA---AEIMPVEYREVFIRLQ-------HE--NKMLRLQQEGSE-NERIE 490
            ..|..|.|.::...|.|   |..:.:|.|.:...|:       ||  :|||.|::|..: :.:||
  Fly   440 DCNSGDNSLSEQLTNNAQTRALKLELENRRLTAALEQLKESSFHESTSKMLELEKEKKKLSLKIE 504

Human   491 ELQEQLEQKHRKMNELETEQRLSKERIRELQQQIEDLQKSLQEQGSKSEGESSSKLKQKL---EA 552
            ::||.:.:..::..|||...:.:.|..::||..:::.|||...|..:.|.:     :|||   |.
  Fly   505 QMQENINRLTQQNVELEGVFKNALEENKKLQDAVDNRQKSYDRQSLEREAD-----RQKLSDAEQ 564

Human   553 HMEKLTEVHEELQKKQELIEDLQPDINQNVQ-KINELEAALQKK------DEDMKAMEERYKMYL 610
            |:|.|.:..:.:|...|.|:....|:.:..: |..|||..|:|.      .:.:..:|.|...|.
  Fly   565 HVETLNKEKQRIQTLNESIQRRADDLERLAESKTKELEQYLEKSRQYELTKQKLYEIEARVSTYE 629

Human   611 EKARNVIKTLDPKLNPASAEIMLLRKQLAEKERRIEILESEC-KVAK-FRDYE---EKLIVSAWY 670
            .:..:::|.:. ||...|.:..:   ||.:...|:::...|. |:.| ..|.|   :||:.....
  Fly   630 RENASLLKEVS-KLKEGSEQKSV---QLDDSINRLDVQSKELQKLGKALEDSEQVHQKLVELEKQ 690

Human   671 NKSLAFQKLGMESRLVS 687
            |:.||.|:: ::..::|
  Fly   691 NQELASQRI-IDQEMIS 706

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HOOK1	NP_056972.1	Sufficient for interaction with microtubules	1..555		141/586 (24%)
		HkD_Hook1	15..163	CDD:411796	42/158 (27%)
		Sufficient for homodimerization, interaction wit HOOK2, HOOK3 and AP4M1. /evidence=ECO:0000269|PubMed:32073997	169..444		63/294 (21%)
		HOOK	186..719	CDD:461694	128/545 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	481..512		8/31 (26%)
		Sufficient for interaction with AKTIP and VPS18. /evidence=ECO:0000269|PubMed:18799622	657..728		9/34 (26%)
Girdin	NP_647780.1	HkD_HkRP	15..165	CDD:411794	42/156 (27%)
		SMC_prok_B	315..1110	CDD:274008	105/416 (25%)