Protein Alignment SMARCAL1 and RAD5

DIOPT Version :10

• Sequence 1: NP_054859.2
• Gene: SMARCAL1 / 50485
• HGNCID: 11102
• Length: 954
• Species: Homo sapiens
• Sequence 2: NP_013132.1
• Gene: RAD5 / 850719
• SGDID: S000004022
• Length: 1169
• Species: Saccharomyces cerevisiae
• Alignment Length: 1234
• Identity: 230/1234 - (18%)
• Similarity: 386/1234 - (31%)
• Gaps: 483/1234 - (39%)

Human    10 RKKIEENRQ---------------KALARRAEKLLAEQHQRTS----------SGTSIAGNPFQA 49
            |::|||..|               |.|.....:....|.:..|          |||||:..|
Yeast    67 RREIEEEGQNQFITELLRIIPEMPKDLVMELNEKFGSQEEGLSLALSHYFDHNSGTSISKIP--- 128

Human    50 KQGPSQNFPRESCKPVSHGVIFKQQNLSSSSNADQRPHDSH-------------SFQAKGIWKKP 101
             ..|:|                 ...||.:||:...|...|             ..:.|..||:
Yeast   129 -SSPNQ-----------------LNTLSDTSNSTLSPSSFHPKRRRIYGFRNQTRLEDKVTWKR- 174

Human   102 EEMPTACPGHSPRSQMALTGISPPLAQSPPEVPKQQLLSYELGQGHAQASPEIRFTPFANPTHKP 166
                       ....:.:||::......|.:...|..|.        ::|.||.    |...:..
Yeast   175 -----------FIGALQVTGMATRPTVRPLKYGSQMKLK--------RSSEEIS----ATKVYDS 216

Human   167 LAKPKSSQETPAHSSGQPPRDAKLEAKTAKASPSGQNISY----IHSSSESVT------------ 215
            ..:.|:|..:.....     |.:.:.:..:.|.....|.|    .|..|..||
Yeast   217 RGRKKASMASLVRIF-----DIQYDREIGRVSEDIAQILYPLLSSHEISFEVTLIFCDNKRLSIG 276

Human   216 -----------------PRTEG-----RLQQKSGSSVQKGVNSQKGKCVRNGDRFQVLIGYNAEL 258
                             .|.:|     |.:.:.|:..:|. |...|:.:...|...........|
Yeast   277 DSFILQLDCFLTSLIFEERNDGESLMKRRRTEGGNKREKD-NGNFGRTLTETDEELESRSKRLAL 340

Human   259 IAVFKTLPSK----------------NYDPDTKTWNFSMND----------YSALMKAAQSLPTV 297
            :.:|..|..|                |.||:....:   ||          ::.|........|:
Yeast   341 LKLFDKLRLKPILDEQKALEKHKIELNSDPEIIDLD---NDEICSNQVTEVHNNLRDTQHEEETM 402

Human   298 NLQPLEWAYGSSESPSTSSEGQAGLPSAPSLSFVKGRCMLISRAYFEADISYSQDLIALFKQMDS 362
            ||..|:..|.:::    |||....||....           ||..|:.::
Yeast   403 NLNQLKTFYKAAQ----SSESLKSLPETEP-----------SRDVFKLEL--------------- 437

Human   363 RRYDVKTRKWSFLLEEHSKLIAKVRCLPQV------QLDPL------PTTLTLAFASQLKKTSLS 415
            |.|..:...|....|:.   .||.....:.      .::||      |..::.| |..|::..::
Yeast   438 RNYQKQGLTWMLRREQE---FAKAASDGEASETGANMINPLWKQFKWPNDMSWA-AQNLQQDHVN 498

Human   416 LTPDV-PEADLSEVDPKLVSNLMPFQRAGVNFAIAKGGRLLLADDMGLGKTIQA----------- 468
            :...: ..|:|...:..|...::.        .:.|||  :|:|:||||||:.|
Yeast   499 VEDGIFFYANLHSGEFSLAKPILK--------TMIKGG--ILSDEMGLGKTVAAYSLVLSCPHDS 553

Human   469 ------------ICIAAFYRKEW----------PLLVVVPSSVRFTWEQAFLRWLPSLSPDCINV 511
                        ..::......|          ..|:|||.|:...|...|.:  .:.|||..:.
Yeast   554 DVVDKKLFDIENTAVSDNLPSTWQDNKKPYASKTTLIVVPMSLLTQWSNEFTK--ANNSPDMYHE 616

Human   512 VVTG-----------------------------------KDRLTAGLINIVSFDLLSKLEKQLKT 541
            |..|                                   |.|:|...:|| |..|.|       .
Yeast   617 VYYGGNVSSLKTLLTKTKTPPTVVLTTYGIVQNEWTKHSKGRMTDEDVNI-SSGLFS-------V 673

Human   542 PFKVVIIDESHFLKNSRTARCRAAMPVLKVAKRVILLSGTPAMSRPAELYT--QIIAVKP----T 600
            .|..:||||.|.::|..|...:|.|.:....|.|  |:|||.::|..:||:  :.:.:.|    .
Yeast   674 NFYRIIIDEGHNIRNRTTVTSKAVMALQGKCKWV--LTGTPIINRLDDLYSLVKFLELDPWRQIN 736

Human   601 FFPQF-----------HAFGL-----------------------------RYCDAKRMPWGWDYS 625
            ::..|           .||.:                             :....||:|    :|
Yeast   737 YWKTFVSTPFESKNYKQAFDVVNAILEPVLLRRTKQMKDKDGKPLVELPPKEVVIKRLP----FS 797

Human   626 GSSNLGELKLLLEEA---------------------VMLRRLK-----------SDVLSQLPAKQ 658
            .|.:| ..|.||::|                     |.:.||:           .|...:..:|.
Yeast   798 KSQDL-LYKFLLDKAEVSVKSGIARGDLLKKYSTILVHILRLRQVCCHPGLIGSQDENDEDLSKN 861

Human   659 RKIV------------VIAPGRINARTRAALDAAAKEMTTK----------------------DK 689
            .|:|            |::....|:.::..|||..:.:..|                      ||
Yeast   862 NKLVTEQTVELDSLMRVVSERFDNSFSKEELDAMIQRLKVKYPDNKSFQSLECSICTTEPMDLDK 926

Human   690 TK--------------------------------QQQKDALIL-----------------FFNRT 705
            ..                                :.|.||..|                 :...:
Yeast   927 ALFTECGHSFCEKCLFEYIEFQNSKNLGLKCPNCRNQIDACRLLALVQTNSNSKNLEFKPYSPAS 991

Human   706 AEAKIPSVIEYILDLLE--SGREKFLVFAHHKVVLDAITQELER---KHVQHI-RIDGSTSSAER 764
            ..:||.::::. |.||:  |..|:.::|:.....||.:.:||..   |.|..| :.||..|..||
Yeast   992 KSSKITALLKE-LQLLQDSSAGEQVVIFSQFSTYLDILEKELTHTFSKDVAKIYKFDGRLSLKER 1055

Human   765 EDLCQQFQLSE--RHAVAVLSITAANMGLTFSSADLVVFAELFWNPGVLIQAEDRVHRIGQTSSV 827
            ..:...|.:.:  |..:.:||:.|..:||..:.|......:.:|:|.:..||.||:||||||:||
Yeast  1056 TSVLADFAVKDYSRQKILLLSLKAGGVGLNLTCASHAYMMDPWWSPSMEDQAIDRLHRIGQTNSV 1120

Human   828 GIHYLVAKGTADDYLWPLIQEKIKVLAEAGLSETNFSEMTESTDYLYKDPKQQKIYDLFQKSFE 891
            .:...:.:.:.::.:. .||||.:.:.||           ..||   :|.::::..:..|..||
Yeast  1121 KVMRFIIQDSIEEKML-RIQEKKRTIGEA-----------MDTD---EDERRKRRIEEIQMLFE 1169

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SMARCAL1	NP_054859.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..20		5/24 (21%)
		Mediates interaction with RPA2. /evidence=ECO:0000269|PubMed:24910198	5..30		7/34 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	27..238		44/271 (16%)
		HARP	248..302	CDD:462166	13/79 (16%)
		HARP	343..397	CDD:462166	7/59 (12%)
		HepA	<423..856	CDD:440319	134/669 (20%)
		DEXHc_HARP_SMARCAL1	436..645	CDD:350768	67/343 (20%)
		DESH box	549..552		2/2 (100%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:18805831	644..661		4/27 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	904..934
RAD5	NP_013132.1	HIRAN	176..284	CDD:400928	19/124 (15%)
		HepA	<527..1143	CDD:440319	130/636 (20%)
		RING-HC_RAD5	909..973	CDD:438493	6/63 (10%)