DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Prosap and shank3b

DIOPT Version :9

Sequence 1:	NP_610925.3	Gene:	Prosap / 50225	FlyBaseID:	FBgn0040752	Length:	1871	Species:	Drosophila melanogaster
Sequence 2:	XP_005164655.1	Gene:	shank3b / 566152	ZFINID:	ZDB-GENE-041210-74	Length:	1643	Species:	Danio rerio

Alignment Length:	1768	Identity:	482/1768 - (27%)
Similarity:	702/1768 - (39%)	Gaps:	523/1768 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 EPPPEPRDGWLLVRIHVPELNVYKCLQFPSERLVWDVKQQVLASLPKELKESFNYGLFAPPANGK 74
            ||..:.....:::||.:|:|...|||:...|..||..||:||.:|.:.|.:..||||:.|..||:
Zfish    44 EPMEDLHGNAVVIRIGIPDLQQTKCLRLDLEAPVWVCKQRVLVTLTQSLTDVLNYGLYLPAFNGR 108

  Fly    75 AGKFLDEERRLGDYPFN--GPVGYLELKYKRRVYKMLTLDERQLKALHTRANLRRFLECINGGHV 137
            |||||||||.|.:||..  .||.|||.:||||||....:|::||..|||:|||::|:|.:....|
Zfish   109 AGKFLDEERLLREYPLPTVTPVPYLEFRYKRRVYTQSHVDDKQLAKLHTKANLKKFMEYVQQRCV 173

  Fly   138 EKIAKMCAKGLDPNFHCSESGDTPLTVATGAKKPNKLLIALVNGGALLDYRTKDGSTALHRAVEH 202
            :|:.:...||||||||.|:||::|||:.........|:..|.:|||.||:||:||.||||:|.:.
Zfish   174 DKVCRFLEKGLDPNFHDSDSGESPLTLVAQLDTCADLIKVLRSGGAHLDFRTRDGLTALHKAAQT 238

  Fly   203 DSLEAVSTLLELGASPNYRDGRGITPLYISITRKCEAKITESLLHDHATLGIQDSQGWNEVHQAC 267
            .:..|::|||:|||||:|:|.||:||||.|.....:....|.||:|||.||..|..||.|:||||
Zfish   239 HNHVALTTLLDLGASPDYKDSRGLTPLYHSAMVGGDPYCCELLLYDHAQLGYSDENGWQEIHQAC 303

  Fly   268 RHGLVQHLEHLLFYGADMDGRNASGNSPLHVCAVNNQEACARMLLFRGAQRGAQNFANQTPYQVA 332
            |||.||||||||||||:|..:|||||:.||:||:.|||.|||:||||||.:..:|:.|||.:|||
Zfish   304 RHGNVQHLEHLLFYGAEMSAQNASGNTALHLCALYNQEGCARVLLFRGANKEIKNYNNQTAFQVA 368

  Fly   333 VIAGNLELAEIIENYKSEDIVPFRGPPRYNPKRRSGIGWLSANGAAGAALLAAAGGGFGGAMVGS 397
            :||||.:|||||:.:|:.|:||||..|.|:.:||:     .......:.:.:|:.......:...
Zfish   369 IIAGNFDLAEIIKIHKTSDVVPFRETPSYSSRRRA-----VCVSPRRSLMRSASDNALDETLTAP 428

  Fly   398 NGAANGNNGAGGVGLMLSHQNHQQHHAGHHQQQHMHHQHHQNHHQQLQQQQLPHLHTLQLHGPPS 462
            :.|                                                 |.|.:|....|..
Zfish   429 SPA-------------------------------------------------PSLRSLPPLEPDD 444

  Fly   463 PCPSEHMLGAYSSASSSLSE--GSSGHRSHEDDISIVTDKSLGDTSDIISDSSGVGTNSDSAACS 525
            ..||:....|..:.:.||..  .|.||                     :|..|.|.......|..
Zfish   445 TVPSQRSPQAAHTHTRSLRRHTRSGGH---------------------LSPGSPVQREPSPPAVP 488

  Fly   526 IGH--------PSTTVVCMEPYAGNTVGHIRLQPGDVIEVVGSTDCGLLEGYVRGTNQSGFFPAD 582
            .|.        |..|.:.:..:.....|.|.|..|:.::|:...:.|..||.|:|  ::|:|||.
Zfish   489 RGPKRRLYSAVPGRTFIAINSHTPQGEGEITLNRGERVKVLSIGEGGFWEGSVKG--RTGWFPAH 551

  Fly   583 CVQEVSLRQKHITNVMTASTGMAPQQQQQQHLQQAPAGSSAASYQGSPQLSLGGHSGSSSTLLQQ 647
            ||:||.:||                                                      ..
Zfish   552 CVEEVQMRQ------------------------------------------------------YD 562

  Fly   648 PHQSPSLSVASNGSCQQPLESNEGGASGNGINNRNNNHSVGQYSSATAPRIKKSAYN----APRS 708
            |.                ||:.|     :.......:::||.|.:.|       :|:    ..:|
Zfish   563 PR----------------LETRE-----DRTKRLFRHYTVGSYDNYT-------SYSDYVIEEKS 599

  Fly   709 VVLH-RAKRGFGFILRGAKASSQLMQLRPSERFPALQYLDDVDPGGVADMAGLRPGDFLLTINGE 772
            ..|. |...||||:||||||.:.:.:..|:..|||||||:.||..|||..||||.||||:.:||.
Zfish   600 ATLQKRDSEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDLEGVAWRAGLRTGDFLIEVNGV 664

  Fly   773 DVTSASHEQVVEMIRSAGALVNLTVVSPQFPHQMQASAQYLPSGARAGSHHLNSGPSTPQSSHRQ 837
            .|....|.|||.:||..|:.:.:.|||
Zfish   665 SVVKVGHRQVVSLIRQGGSRLVMKVVS-------------------------------------- 691

  Fly   838 CATLPRKMTGPGGSGPGSSSGGSVR-MAPMPPRRDPKTTLSVGRARAKSMVAGLE------NGGE 895
               :.||          ..:|..|| .||.||:|||.|:|::   |:|||.|.||      ..||
Zfish   692 ---VTRK----------PDTGDVVRKKAPPPPKRDPSTSLTL---RSKSMTAELEELASRRRRGE 740

  Fly   896 KEDDLPHTKSNSVESIATPTPTGIQTGPGTPVQLRTASIKARPTSSRITAAELEELFQRQ----- 955
            |.|::   .|:..|.:.......:.:      ..|.|::|.||||.|||.||:..|.:||
Zfish   741 KLDEM---LSSPKEQVVVMRQRPVDS------DSRAATVKQRPTSRRITQAEISSLLERQGLPIS 796

  Fly   956 --------------QGEG-----SAANASRYATMMTSSRF---QSGTDS----------GAATPP 988
                          :|..     .|....|.:.::...||   .|.|||          ..|.||
Zfish   797 ELSLAVDKSHMQLPRGMSRTKSFGAPEDDRISALIGEHRFPRSSSMTDSFRQDSIPPPPQTAPPP 861

  Fly   989 -----------------------ASN--GSPMRSG--PLVYGSVAEMKRKTARSK---HGSGTLR 1023
                                   |:|  .|..|.|  |.::|.|...:::..||.   ..:..|.
Zfish   862 PPTPYFLDSGPPPSFLPPPPPSRAANQSRSSFRPGAEPKIHGPVTTDRQRKTRSMIILQDTSHLP 926

  Fly  1024 GKPV-----ATPTVGPGGAGGGRDLKRFHSTPDLHGPQLH-GSASSIWQAS-------------- 1068
            .:|.     .|||.||.....||     ...|.:..|..: |..|:::..:
Zfish   927 VEPTPITRPQTPTSGPVPPERGR-----RRGPPVENPYANVGRLSAVYTPTKPQRRKSPLVKQGP 986

  Fly  1069 ---GKGHHSQDDVATL------HAS-----LQRLNSNQGELKLGGLGAGSATGA----------- 1108
               |...||..|.:.|      |:|     .|::.|.:..:        :..||
Zfish   987 VEEGAAAHSSRDPSPLGGSRIPHSSRAEQFQQQVLSERARI--------TPPGARRRPSVFLSVE 1043

  Fly  1109 GGAVLP------------------PPNHP---PPPPPVGQVVKVETRSSVSEYESTISLQQKLKK 1152
            |||..|                  ||..|   |.|||.|............:..|.::|....::
Zfish  1044 GGATEPQTTPLLSQSHSVDELAELPPPAPMLSPGPPPGGTTFIHPLTGRPLDPSSPLALALAARE 1108

  Fly  1153 RTENDAVTSAAIDGVQSSFNPSANAKIYASPQELRNVMAWKLRQAQEKPSQETSAGSQQPVSQYA 1217
            |         |:.|..:   |:.......||.:.|         |.|:|  ||..|:..|....|
Zfish  1109 R---------ALSGRNT---PTPTPSPTPSPTQGR---------AVERP--ETEGGATPPAPLEA 1150

  Fly  1218 APTQM---RPAQQQQQAQQ-----PPTALASHYAAPQVQVQQVQQVQQSPQQSAPQSPPAPPLPQ 1274
            .|:..   .|....:.|.|     |.:..:...|.....||.:|......:.:.|..||.  ||.
Zfish  1151 PPSNSWRDEPVSITETASQVTSGSPGSGRSLEEALAPPGVQNIQPALMDTEHTPPAVPPT--LPS 1213

  Fly  1275 AAPVPAQNGNGNGSTSGAGSAPP----IPEPDYSCSESDGEDE--------------NSILVARN 1321
            .||..:.....:.:.|....|.|    :|....|.|:.:..:|              |.:|:...
Zfish  1214 PAPTLSNLTARSMTMSSEEEAEPYTVTLPPALLSSSDEETREELRKIGLVPPPQPFANGLLIKET 1278

  Fly  1322 TKLNEKIALFDVPETSGNSQASGSSSNSGSASIS---------HSLSVEEIQRIRS-NLKTSKSS 1376
            :|....|:      .|| |:.|.:.::||.||.|         |..:...:..:.| :..:|:|:
Zfish  1279 SKATLSIS------PSG-SRPSIAKTSSGKASDSTADSGVEDPHMETTSTVSTVSSMSTLSSEST 1336

  Fly  1377 PNGFAKKP----------EEEKPQQEQQQSHQ-----------QPQQLLQP-----GEDECDNSS 1415
            .:..|.||          ....|..:|....:           :|:.|.|.     ||:  ..:.
Zfish  1337 DSAHASKPRCGVGRGRPAHLRDPLLKQSSDSELLPHPPSTGPSRPRYLFQRRSKLWGEE--PRAQ 1399

  Fly  1416 SGVSSEQEQLALAAGVTLPGGGKPTDTIKKKPSVTIVEEPKTIPDQPSSNTSHTTKPMAKTTISI 1480
            .|.|.|....|:.|.:.    .|.|.::.::|       |...|..|...:     |:.......
Zfish  1400 MGSSDESRPAAMGAELL----SKDTHSLGEEP-------PMGAPLDPGRRS-----PVGGARCEE 1448

  Fly  1481 GGGGSAVPTATL-TVKQLVQQQHAPVIQQQQQQLGSKQPVT--AASTNKFTQQSNINSNVMSPQV 1542
            .||.|....::| .:..:.|:.:......:.   ||:.|||  ..|.....::|         :.
Zfish  1449 NGGESKSLFSSLGELHTISQRSYGTTFTIRP---GSRYPVTRRTPSPGATPERS---------EP 1501

  Fly  1543 LGRI----PSHHH 1551
            ||.:    |.|||
Zfish  1502 LGPVRTFGPHHHH 1514

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Prosap	NP_610925.3	FERM_f0	20..103	CDD:293120	41/84 (49%)
		Ank_2	128..222	CDD:289560	44/93 (47%)
		ANK	131..233	CDD:238125	49/101 (49%)
		ANK repeat	157..189	CDD:293786	12/31 (39%)
		ANK	186..312	CDD:238125	75/125 (60%)
		ANK repeat	191..222	CDD:293786	17/30 (57%)
		Ank_2	196..322	CDD:289560	74/125 (59%)
		ANK repeat	224..289	CDD:293786	39/64 (61%)
		ANK repeat	291..322	CDD:293786	19/30 (63%)
		ANK repeat	324..353	CDD:293786	17/28 (61%)
		SH3	532..586	CDD:302595	17/53 (32%)
		PDZ_signaling	706..798	CDD:238492	44/92 (48%)
shank3b	XP_005164655.1	FERM_f0	55..126	CDD:293120	35/70 (50%)
		ANK repeat	193..225	CDD:293786	12/31 (39%)
		ANK	222..348	CDD:238125	75/125 (60%)
		ANK repeat	227..258	CDD:293786	17/30 (57%)
		Ank_4	228..282	CDD:290365	25/53 (47%)
		ANK repeat	260..325	CDD:293786	39/64 (61%)
		Ank_2	299..388	CDD:289560	58/88 (66%)
		ANK repeat	327..358	CDD:293786	19/30 (63%)
		SH3	502..553	CDD:302595	15/52 (29%)
		PDZ_signaling	601..690	CDD:238492	43/88 (49%)
		SAM_Shank1,2,3	1576..1641	CDD:188905
		SAM	1580..1643	CDD:197735
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170582293
Domainoid	1	1.000	54	1.000	Domainoid score	I11194
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	573	1.000	Inparanoid score	I1015
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001441
OrthoInspector	1	1.000	-	-		otm25540
orthoMCL	1	0.900	-	-		OOG6_103134
Panther	1	1.100	-	-	O	PTHR24135
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X750
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.890