Protein Alignment Prosap and SHANK1

DIOPT Version :10

• Sequence 1: NP_610925.3
• Gene: Prosap / 50225
• FlyBaseID: FBgn0040752
• Length: 1871
• Species: Drosophila melanogaster
• Sequence 2: XP_011525315.1
• Gene: SHANK1 / 50944
• HGNCID: 15474
• Length: 2169
• Species: Homo sapiens
• Alignment Length: 2065
• Identity: 517/2065 - (25%)
• Similarity: 746/2065 - (36%)
• Gaps: 632/2065 - (30%)

  Fly    12 PPEPRDGWLLVRIHVPELNVYKCLQFPSERLVWDVKQQVLASLPKELKESFNYGLFAPPANGKAG 76
            |.:.....::.||.:|:|:..|||:|..:..:|..|||||.:|.:.|::..|||||.|..:|:..
Human    65 PDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDA 129

  Fly    77 KFLDEERRLGDYP--FNGPVGYLELKYKRRVYKMLTLDERQLKALHTRANLRRFLECINGGHVEK 139
            .||:|||.|.:||  |...|.|||.:||.||||...|||:||..|||:..|::|||.:..|..:|
Human   130 NFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDK 194

  Fly   140 IAKMCAKGLDPNFHCSESGDTPLTVATGAKKPNKLLIALVNGGALLDYRTKDGSTALHRAVEHDS 204
            :|::..||||||:|.|:||:||||:|...:...:::..|..|||.:|:|.:||.||||:|.....
Human   195 VARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARH 259

  Fly   205 LEAVSTLLELGASPNYRDGRGITPLYISITRKCEAKITESLLHDHATLGIQDSQGWNEVHQACRH 269
            ..|::.||:||.||||:|.||:|||:.:.....:.:..|.||.:.|.|||.|..||.|:||||:.
Human   260 CLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLFNRAQLGIADENGWQEIHQACQR 324

  Fly   270 GLVQHLEHLLFYGADMDGRNASGNSPLHVCAVNNQEACARMLLFRGAQRGAQNFANQTPYQVAVI 334
            |..|||||||||||:...:|||||:.||:||:.|:|.|||:||:|||.:..:|...|||:|||||
Human   325 GHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGADKDVKNNNGQTPFQVAVI 389

  Fly   335 AGNLELAEIIENYKSEDIVPFRGPPRYNPKRRS--GIG------WLSANGAAGAAL-----LAAA 386
            |||.||.|:|.|::.:|:|||:..|:|..:||.  |.|      .|.||.....||     .:|.
Human   390 AGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGTGLTVPPALLRANSDTSMALPDWMVFSAP 454

  Fly   387 GGGFGGAMVGSNGAANGNNGAGGVGLMLSHQNHQQHHAGHHQQQHMHHQHHQNHHQQLQQQQLPH 451
                |.|..|:.|..:|:.|                                        |..|.
Human   455 ----GAASSGAPGPTSGSQG----------------------------------------QSQPS 475

  Fly   452 LHTLQLHGPPSPCPSEHMLGAYSSASSSLSEGSSGHRSHEDDISIVTDKSLGDTSDIISDSSGV- 515
            ..|.:|        |...|.:.||...:.:...|..|..||    ...:..|..|     |||.
Human   476 APTTKL--------SSGTLRSASSPRGARARSPSRGRHPED----AKRQPRGRPS-----SSGTP 523

  Fly   516 ------GT-NSDSAACSIGH-----------PSTTVVCMEPYAGNTVGHIRLQPGDVIEVVGSTD 562
                  || .|.....|:|.           |..:.:.::.|.....|.|.|..|:.|:|:...:
Human   524 REGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQAEGEISLSKGEKIKVLSIGE 588

  Fly   563 CGLLEGYVRGTNQSGFFPADCVQEVSLRQKHITNVMTASTGMAPQQQQQQHLQQAPAGSSAASYQ 627
            .|..||.|:|  :.|:||:||::||:.|.               |:.:|:
Human   589 GGFWEGQVKG--RVGWFPSDCLEEVANRS---------------QESKQE--------------- 621

  Fly   628 GSPQLSLGGHSGSSSTLLQQPHQSPSLSVASNGSCQQPLESNEGGASGNGINNRNNNHSVGQYSS 692
                    ..|..:..|.:                                     :::||.|.|
Human   622 --------SRSDKAKRLFR-------------------------------------HYTVGSYDS 641

  Fly   693 ATAPRIKK-----SAYNAPRSVVLHRAK--RGFGFILRGAKASSQLMQLRPSERFPALQYLDDVD 750
            ..||.:..     |.|......||.:.|  .||||:||||||.:.:.:..|:..|||||||:.||
Human   642 FDAPSLMDGIGPGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVD 706

  Fly   751 PGGVADMAGLRPGDFLLTINGEDVTSASHEQVVEMIRSAGALVNLTVVSPQFPHQMQASAQYLPS 815
            .||||..||||.||||:.:||::|....|.|||.|||..|..:.:.||.
Human   707 EGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVM---------------- 755

  Fly   816 GARAGSHHLNSGPSTPQSSHRQCATLPRKMTGPGGSGPGSSSGGSVRMAPMPPRRDPKTTLSVGR 880
                    :...|...::.|::                          ||...:|.|..|:|:
Human   756 --------VTRHPDMDEAVHKK--------------------------APQQAKRLPPPTISL-- 784

  Fly   881 ARAKSMVAGLENGGEKEDDLPHTKSNSVESIATPTPT---------------------GIQT--- 921
             |:|||.:.||     |...|..|.:..|....|.|:                     ..||
Human   785 -RSKSMTSELE-----EMVSPWKKKSEYEQQPAPVPSMEKKRTVYQMALNKLDEILAAAQQTISA 843

  Fly   922 ----GPGTPVQLRTASIKARPTSSRITAAELEE-------------------LFQRQQGEGSAAN 963
                |||....|.    |.||.....|.:..:.                   |..||:..|:|.:
Human   844 SESPGPGGLASLG----KHRPKGFFATESSFDPHHRAQPSYERPSFLPPGPGLMLRQKSIGAAED 904

  Fly   964 ASRYAT--MMTSSRFQSGTDSGAATPPASNGSPMRSGPLVYGSVAEMKRKTARSKHGSGTLRGKP 1026
            ...|..  .|..||..|...|....||.:...|            |....|......||.|...|
Human   905 DRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPP------------EPPYSTPPVPSSSGRLTPSP 957

  Fly  1027 VATPTVGPGGAG--------------------GGRDLKRFHSTP----DLHGPQLHGSASSIWQA 1067
            ...| ..||..|                    |.|:...:||.|    ..|.|..|
Human   958 RGGP-FNPGSGGPLPASSPASFDGPSPPDTRVGSREKSLYHSGPLPPAHHHPPHHH--------- 1012

  Fly  1068 SGKGHHSQDDVATLHASLQRLNSNQGELKLGGLGAGSATGAGGAVLPPPN------HPPPPPPVG 1126
                ||        ||                              |||.      |||.||
Human  1013 ----HH--------HA------------------------------PPPQPHHHHAHPPHPP--- 1032

  Fly  1127 QVVKVETRSSVSEYESTISLQQKLKKR------------TENDAVTSAAIDGVQSSFNP------ 1173
               ::||..|..:....::|..:...|            ..:.:...:|..|..||..|
Human  1033 ---EMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTPGAPSPSHHGSAGGGGGSSQGPALRYFQ 1094

  Fly  1174 ----SANAKIYASPQELRNVMAWKLRQAQEKPSQETSAGSQQPVSQYAAPTQMRPAQQQQQAQQP 1234
                :|:|.:|...:..|......::|.  |...|...|...|    .||:...||     :.||
Human  1095 LPPRAASAAMYVPARSGRGRKGPLVKQT--KVEGEPQKGGGLP----PAPSPTSPA-----SPQP 1148

  Fly  1235 PTALASHYAAPQVQVQQV----QQVQQSPQQSAPQSPPAPPLPQAAPVPAQNGNGNGSTSGAGSA 1295
            |.|:|:......:.:..:    .....|.:.|...|..|.|..|  |.|...|.|.||:.....|
Human  1149 PPAVAAPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQ--PEPTGGGGGGGSSPSPAPA 1211

  Fly  1296 ----PPIPEPDYSCSESDGE---DENS------ILVAR-----NTKLNEKIALF---DVPETSGN 1339
                ||.|.|..:.:...|.   |..|      :..||     ..:...:..||   |..:..|.
Human  1212 MSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGG 1276

  Fly  1340 SQASGSSSNSGSASISHSLSVEE-------IQRIRSNLKTSKSSPNGFAKKPEEEKPQQEQQQSH 1397
            ....|:.:..| ..:.||.|::|       ..|:.|....:.....|...:............|.
Human  1277 DGGLGTGAAPG-PRLRHSKSIDEGMFSAEPYLRLESAGSGAGYGGYGAGSRAYGGGGGSSAFTSF 1340

  Fly  1398 QQPQQLLQPGEDECDNSSSGVSSEQEQLALAA-----GVTLPGGGKPTDTIKKKPSVTIVEEPKT 1457
            ..|:.|:.|...:..:.:|.:.     |||||     ..:..|||.|..  ..:|.....|.|..
Human  1341 LPPRPLVHPLTGKALDPASPLG-----LALAARERALKESSEGGGAPQP--PPRPPSPRYEAPPP 1398

  Fly  1458 IPDQPSSNTSHTTKPMAKT---------------TISIGGGGSAVPTATLTVKQLVQQQ---HAP 1504
            .|...|.:..|  :|:.:.               ...:|....::|.:....::.:..:   .||
Human  1399 TPHHHSPHAHH--EPVLRLWGASPPDPARRELGYRAGLGSQEKSLPASPPAARRSLLHRLPPTAP 1461

  Fly  1505 VIQQQQQQLGSKQPVTAASTNKFTQQSNINSNVMSPQVLGRIPSHHHQQQSSNPNQKLIATQQQI 1569
            .:.....|||::.|......:|..:.:       :|:...|:|.|....::..|
Human  1462 GVGPLLLQLGTEPPAPHPGVSKPWRSA-------APEEPERLPLHVRFLENCQP----------- 1508

  Fly  1570 LQQQQQQLAHQQHLQQILKAKAAAAGGASNTAVLVAKHQQKLHKGTSSGHESEMETRSDLEDDDG 1634
                                :|....|                :|..|            ||..|
Human  1509 --------------------RAPVTSG----------------RGPPS------------EDGPG 1525

  Fly  1635 --------DLSPSPPAKAFQRHNSL----TRKQAAAIAMQRGATRTTAVSLMQLPPPLEADSDGE 1687
                    .:.|||.:......|.|    ....|.::.::.|    ..:.:..||||||..:..|
Human  1526 VPPPSPRRSVPPSPTSPRASEENGLPLLVLPPPAPSVDVEDG----EFLFVEPLPPPLEFSNSFE 1586

  Fly  1688 PSQLTLQRQQSHHPSQTHPHPHQLQQQLQLQQQQQQQQQQQQPMAAHIVGMLPSGQLVAVASGAV 1752
            ..:..|.      |...||.|                   ..|        .|:..|..|...||
Human  1587 KPESPLT------PGPPHPLP-------------------DTP--------APATPLPPVPPPAV 1618

  Fly  1753 AGVPGV---GATAVQQGNNNLQQLCTDNLVLAP-------PPQFCDCNDAKHAPQPHLPTSQYHP 1807
            |..|..   .|:::...::.:..| |.....||       ||       |..||.|..|.....|
Human  1619 AAAPPTLDSTASSLTSYDSEVATL-TQGASAAPGDPHPPGPP-------APAAPAPAAPQPGPDP 1675

  Fly  1808 QQQQQQQHQQQQQQQQQLQQQLQQQQMLQMHQRLS--------GGAGAGA 1849
            ........::...:... ...|:..........||        ||.||||
Human  1676 PPGTDSGIEEVDSRSSS-DHPLETISSASTLSSLSAEGGGSAGGGGGAGA 1724

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Prosap	NP_610925.3	FERM_F0_SHANK	20..103	CDD:340611	37/84 (44%)
		ANKYR	109..348	CDD:440430	121/238 (51%)
		ANK repeat	157..189	CDD:293786	12/31 (39%)
		ANK repeat	191..222	CDD:293786	16/30 (53%)
		ANK repeat	224..289	CDD:293786	32/64 (50%)
		ANK repeat	291..322	CDD:293786	17/30 (57%)
		ANK repeat	324..353	CDD:293786	17/28 (61%)
		SH3	532..586	CDD:473055	17/53 (32%)
		PDZ_SHANK1_3-like	703..800	CDD:467228	49/98 (50%)
SHANK1	XP_011525315.1	FERM_F0_SHANK1	70..158	CDD:340695	37/87 (43%)
		ANKYR	166..407	CDD:440430	121/240 (50%)
		ANK repeat	212..244	CDD:293786	12/31 (39%)
		ANK repeat	246..277	CDD:293786	16/30 (53%)
		ANK repeat	279..344	CDD:293786	32/64 (50%)
		ANK repeat	346..377	CDD:293786	17/30 (57%)
		SH3	557..608	CDD:473055	16/52 (31%)
		PDZ_SHANK1_3-like	656..757	CDD:467228	49/124 (40%)
		SAM_Shank1,2,3	2102..2167	CDD:188905