Protein Alignment GCS2alpha and ROT2

DIOPT Version :10

• Sequence 1: NP_652145.1
• Gene: GCS2alpha / 49953
• FlyBaseID: FBgn0027588
• Length: 924
• Species: Drosophila melanogaster
• Sequence 2: NP_009788.3
• Gene: ROT2 / 852530
• SGDID: S000000433
• Length: 954
• Species: Saccharomyces cerevisiae
• Alignment Length: 989
• Identity: 334/989 - (33%)
• Similarity: 512/989 - (51%)
• Gaps: 159/989 - (16%)

  Fly    27 KTCEQSSFCRRSR----KIQGSGSKYALIPGTLNTYADSLTADLVNKENHH-------------- 73
            |.|.||.||.|:|    .|..|...|..:.      |:|:..|.:....|.
Yeast    27 KKCAQSGFCHRNRVYAENIAKSHHCYYKVD------AESIAHDPLENVLHATIIKTIPRLEGDDI 85

  Fly    74 --QFAFKLEALVGSTFRLQIDEK--------------QPLRPRYRVEHALKGPTQAGRIRV---- 118
              ||.|.|..|...:.|..|:||              |.....::.....|...:|.|..:
Yeast    86 AVQFPFSLSFLQDHSVRFTINEKERMPTNSSGLLISSQRFNETWKYAFDKKFQEEANRTSIPQFH 150

  Fly   119 ---QRET-------------------------DGEIVITSEKNKAVIHGDPFRIDFFENDVLVVS 155
               |::|                         :|::.:.       |..:||::..:..:.|.:.
Yeast   151 FLKQKQTVNSFWSKISSFLSLSNSTADTFHLRNGDVSVE-------IFAEPFQLKVYWQNALKLI 208

  Fly   156 VNAKNWLYFEHLRQKAQEPTSHPAENENQQEQDAAVETPKAADTIDDPGAWEENF-KSHHDSKPY 219
            ||.:|:|..||.|.| ||..:|....|.....                  :::|| .|.|||.|.
Yeast   209 VNEQNFLNIEHHRTK-QENFAHVLPEETTFNM------------------FKDNFLYSKHDSMPL 254

  Fly   220 GPEAVALDFSFPAAKVLFGIPEHADSFILKSTS-GTDPYRLYNLDVFEYVVDSKMALYGSVPVIY 283
            |||:|||||||..:..::||||||.|..|..|| |.:||||:|:|||||.:.:...:|||:|.::
Yeast   255 GPESVALDFSFMGSTNVYGIPEHATSLRLMDTSGGKEPYRLFNVDVFEYNIGTSQPMYGSIPFMF 319

  Fly   284 GHGAQRTAGVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGP 348
               :..:..::|.|||:|||||:            :.:...||   ..|::||:|::|..:.|||
Yeast   320 ---SSSSTSIFWVNAADTWVDIK------------YDTSKNKT---MTHWISENGVIDVVMSLGP 366

  Fly   349 KPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDG 413
            .......::..|||...||.:.::.|||.|||||||.||.:|.::.|.:.||.|.:|||:|:|:.
Yeast   367 DIPTIIDKFTDLTGRPFLPPISSIGYHQCRWNYNDEMDVLTVDSQMDAHMIPYDFIWLDLEYTND 431

  Fly   414 KRYFTWDKFKFPHPLAMIKNLTELGRHLVVIVDPHIKRDNNYFFHRDCTDRGYYVKTREGNDYEG 478
            |:||||.:..||:|..::..|.:|||:|||::|||:|:|  |.......:....||...||||.|
Yeast   432 KKYFTWKQHSFPNPKRLLSKLKKLGRNLVVLIDPHLKKD--YEISDRVINENVAVKDHNGNDYVG 494

  Fly   479 WCWPGAASYPDFFNPVVREYYASQYALDKFQTVTAD---VMLWNDMNEPSVFNGPEITAPKDLIH 540
            .||||.:.:.|..:...::.:.|.:  ::|..:.||   :.:||||||||:|:|||.||||||||
Yeast   495 HCWPGNSIWIDTISKYGQKIWKSFF--ERFMDLPADLTNLFIWNDMNEPSIFDGPETTAPKDLIH 557

  Fly   541 YGNWEHRDVHNLYGHMHLMGSFAGLQQ--RDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHL 603
            ....|.|.|||:||......::..::.  ...::|||:||||.||||||.||.|||||.|:|.:|
Yeast   558 DNYIEERSVHNIYGLSVHEATYDAIKSIYSPSDKRPFLLTRAFFAGSQRTAATWTGDNVANWDYL 622

  Fly   604 QHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPE 668
            :.|:.|.|:..:||..|.|||:..|..:|..||:.||||.|.:.||||||||||||||||:||.|
Yeast   623 KISIPMVLSNNIAGMPFIGADIAGFAEDPTPELIARWYQAGLWYPFFRAHAHIDTKRREPYLFNE 687

  Fly   669 RTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDR-LLVR 732
            ..:.::::.:..||..||..||.|::..:||.|::.|:..::|...|.:.:|||....:. |||:
Yeast   688 PLKSIVRDIIQLRYFLLPTLYTMFHKSSVTGFPIMNPMFIEHPEFAELYHIDNQFYWSNSGLLVK 752

  Fly   733 PVMQQGVSKVDVYFPAIDDKKNGDWWYDVDTYQRQERSG------YVSVPVDDFKIPVWQRGGSI 791
            ||.:.|.|:.::.||.       ..:|:..:......:|      .:|.|:|  |||::..||.|
Yeast   753 PVTEPGQSETEMVFPP-------GIFYEFASLHSFINNGTDLIEKNISAPLD--KIPLFIEGGHI 808

  Fly   792 VPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSYAYRQGQRIHVNYEFAHDQLVNS 856
            :..|::.||:|.||.:|||.::|..|.:|:|.|.||:||.:::.|::|:.:...:.|.::.|.|.
Yeast   809 ITMKDKYRRSSMLMKNDPYVIVIAPDTEGRAVGDLYVDDGETFGYQRGEYVETQFIFENNTLKNV 873

  Fly   857 FVGKPK------YKTL--AWIERIVIA--GLE---KVPSSASITVNGVSQQLEV---LHHDNTVV 905
            ....|:      :.||  ..||:|:||  .|:   .:..|..:..||....|..   ..:||.:.
Yeast   874 RSHIPENLTGIHHNTLRNTNIEKIIIAKNNLQHNITLKDSIKVKKNGEESSLPTRSSYENDNKIT 938

  Fly   906 VRKPGVKMDVDFAI 919
            :....:.:..|:.:
Yeast   939 ILNLSLDITEDWEV 952

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
GCS2alpha	NP_652145.1	GH31_N	224..362	CDD:270212	52/138 (38%)
		GH31_GANC_GANAB_alpha	362..833	CDD:269889	203/482 (42%)
		DUF5110	809..877	CDD:465360	24/77 (31%)
ROT2	NP_009788.3	GH31_N	259..380	CDD:270212	52/138 (38%)
		GH31_GANC_GANAB_alpha	380..850	CDD:269889	203/482 (42%)