DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment GCS2alpha and ROT2

DIOPT Version :9

Sequence 1:	NP_652145.1	Gene:	GCS2alpha / 49953	FlyBaseID:	FBgn0027588	Length:	924	Species:	Drosophila melanogaster
Sequence 2:	NP_009788.3	Gene:	ROT2 / 852530	SGDID:	S000000433	Length:	954	Species:	Saccharomyces cerevisiae

Alignment Length:	989	Identity:	334/989 - (33%)
Similarity:	512/989 - (51%)	Gaps:	159/989 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 KTCEQSSFCRRSR----KIQGSGSKYALIPGTLNTYADSLTADLVNKENHH-------------- 73
            |.|.||.||.|:|    .|..|...|..:.      |:|:..|.:....|.
Yeast    27 KKCAQSGFCHRNRVYAENIAKSHHCYYKVD------AESIAHDPLENVLHATIIKTIPRLEGDDI 85

  Fly    74 --QFAFKLEALVGSTFRLQIDEK--------------QPLRPRYRVEHALKGPTQAGRIRV---- 118
              ||.|.|..|...:.|..|:||              |.....::.....|...:|.|..:
Yeast    86 AVQFPFSLSFLQDHSVRFTINEKERMPTNSSGLLISSQRFNETWKYAFDKKFQEEANRTSIPQFH 150

  Fly   119 ---QRET-------------------------DGEIVITSEKNKAVIHGDPFRIDFFENDVLVVS 155
               |::|                         :|::.:.       |..:||::..:..:.|.:.
Yeast   151 FLKQKQTVNSFWSKISSFLSLSNSTADTFHLRNGDVSVE-------IFAEPFQLKVYWQNALKLI 208

  Fly   156 VNAKNWLYFEHLRQKAQEPTSHPAENENQQEQDAAVETPKAADTIDDPGAWEENF-KSHHDSKPY 219
            ||.:|:|..||.|.| ||..:|....|.....                  :::|| .|.|||.|.
Yeast   209 VNEQNFLNIEHHRTK-QENFAHVLPEETTFNM------------------FKDNFLYSKHDSMPL 254

  Fly   220 GPEAVALDFSFPAAKVLFGIPEHADSFILKSTS-GTDPYRLYNLDVFEYVVDSKMALYGSVPVIY 283
            |||:|||||||..:..::||||||.|..|..|| |.:||||:|:|||||.:.:...:|||:|.::
Yeast   255 GPESVALDFSFMGSTNVYGIPEHATSLRLMDTSGGKEPYRLFNVDVFEYNIGTSQPMYGSIPFMF 319

  Fly   284 GHGAQRTAGVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGP 348
               :..:..::|.|||:|||||:            :.:...||   ..|::||:|::|..:.|||
Yeast   320 ---SSSSTSIFWVNAADTWVDIK------------YDTSKNKT---MTHWISENGVIDVVMSLGP 366

  Fly   349 KPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDG 413
            .......::..|||...||.:.::.|||.|||||||.||.:|.::.|.:.||.|.:|||:|:|:.
Yeast   367 DIPTIIDKFTDLTGRPFLPPISSIGYHQCRWNYNDEMDVLTVDSQMDAHMIPYDFIWLDLEYTND 431

  Fly   414 KRYFTWDKFKFPHPLAMIKNLTELGRHLVVIVDPHIKRDNNYFFHRDCTDRGYYVKTREGNDYEG 478
            |:||||.:..||:|..::..|.:|||:|||::|||:|:|  |.......:....||...||||.|
Yeast   432 KKYFTWKQHSFPNPKRLLSKLKKLGRNLVVLIDPHLKKD--YEISDRVINENVAVKDHNGNDYVG 494

  Fly   479 WCWPGAASYPDFFNPVVREYYASQYALDKFQTVTAD---VMLWNDMNEPSVFNGPEITAPKDLIH 540
            .||||.:.:.|..:...::.:.|.:  ::|..:.||   :.:||||||||:|:|||.||||||||
Yeast   495 HCWPGNSIWIDTISKYGQKIWKSFF--ERFMDLPADLTNLFIWNDMNEPSIFDGPETTAPKDLIH 557

  Fly   541 YGNWEHRDVHNLYGHMHLMGSFAGLQQ--RDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHL 603
            ....|.|.|||:||......::..::.  ...::|||:||||.||||||.||.|||||.|:|.:|
Yeast   558 DNYIEERSVHNIYGLSVHEATYDAIKSIYSPSDKRPFLLTRAFFAGSQRTAATWTGDNVANWDYL 622

  Fly   604 QHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPE 668
            :.|:.|.|:..:||..|.|||:..|..:|..||:.||||.|.:.||||||||||||||||:||.|
Yeast   623 KISIPMVLSNNIAGMPFIGADIAGFAEDPTPELIARWYQAGLWYPFFRAHAHIDTKRREPYLFNE 687

  Fly   669 RTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDR-LLVR 732
            ..:.::::.:..||..||..||.|::..:||.|::.|:..::|...|.:.:|||....:. |||:
Yeast   688 PLKSIVRDIIQLRYFLLPTLYTMFHKSSVTGFPIMNPMFIEHPEFAELYHIDNQFYWSNSGLLVK 752

  Fly   733 PVMQQGVSKVDVYFPAIDDKKNGDWWYDVDTYQRQERSG------YVSVPVDDFKIPVWQRGGSI 791
            ||.:.|.|:.::.||.       ..:|:..:......:|      .:|.|:|  |||::..||.|
Yeast   753 PVTEPGQSETEMVFPP-------GIFYEFASLHSFINNGTDLIEKNISAPLD--KIPLFIEGGHI 808

  Fly   792 VPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSYAYRQGQRIHVNYEFAHDQLVNS 856
            :..|::.||:|.||.:|||.::|..|.:|:|.|.||:||.:::.|::|:.:...:.|.::.|.|.
Yeast   809 ITMKDKYRRSSMLMKNDPYVIVIAPDTEGRAVGDLYVDDGETFGYQRGEYVETQFIFENNTLKNV 873

  Fly   857 FVGKPK------YKTL--AWIERIVIA--GLE---KVPSSASITVNGVSQQLEV---LHHDNTVV 905
            ....|:      :.||  ..||:|:||  .|:   .:..|..:..||....|..   ..:||.:.
Yeast   874 RSHIPENLTGIHHNTLRNTNIEKIIIAKNNLQHNITLKDSIKVKKNGEESSLPTRSSYENDNKIT 938

  Fly   906 VRKPGVKMDVDFAI 919
            :....:.:..|:.:
Yeast   939 ILNLSLDITEDWEV 952

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
GCS2alpha	NP_652145.1	GH31_N	224..362	CDD:270212	52/138 (38%)
		Glyco_hydro_31	343..792	CDD:279404	189/460 (41%)
		GH31_GANC_GANAB_alpha	362..833	CDD:269889	203/482 (42%)
ROT2	NP_009788.3	GH31_N	259..380	CDD:270212	52/138 (38%)
ROT2	NP_009788.3	GH31_GANC_GANAB_alpha	380..850	CDD:269889	203/482 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157343617
Domainoid	1	1.000	366	1.000	Domainoid score	I122
eggNOG	1	0.900	-	-		E1_COG1501
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H5426
Inparanoid	1	1.050	537	1.000	Inparanoid score	I178
Isobase	1	0.950	-	0	Normalized mean entropy	S1104
OMA	1	1.010	-	-		QHG54072
OrthoFinder	1	1.000	-	-		FOG0000251
OrthoInspector	1	1.000	-	-		oto100380
orthoMCL	1	0.900	-	-		OOG6_100592
Panther	1	1.100	-	-	LDO	PTHR22762
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R499
SonicParanoid	1	1.000	-	-		X1016
TreeFam	1	0.960	-	-
	16	15.740