DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment alpha-Catr and VCL

DIOPT Version :9

Sequence 1:	NP_726389.4	Gene:	alpha-Catr / 49713	FlyBaseID:	FBgn0029105	Length:	810	Species:	Drosophila melanogaster
Sequence 2:	NP_054706.1	Gene:	VCL / 7414	HGNCID:	12665	Length:	1134	Species:	Homo sapiens

Alignment Length:	1140	Identity:	172/1140 - (15%)
Similarity:	323/1140 - (28%)	Gaps:	485/1140 - (42%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 AVERFITIGEIIAEENID--IKGDMYEAAKEARDAGKSIERLCDISPLSGMELRHHIEPLKDYGA 100
            ||...:.:|:...:...|  :|.||..|..:..:|...:.:...:     ::...:..|.:||
Human    50 AVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQM-----LQSDPYSVPARDY-- 107

  Fly   101 IILAARSLLSSVTRILLLVDIIVVKKLLTAKKRASESLEKLESVMNFTEFVRAFSVFGTEMIELA 165
            :|..:|.:||..:.:||..|...|:|::...|...|.|...|.|....:.|......|..|.::|
Human   108 LIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMA 172

  Fly   166 YLTGHHRNSFKEERRRAQMFSARQILEKSIAILLTSSKNSLIHSDCVIVKENRDTVFCQIRRAMD 230
            .:....:.....:..|..:.::...:::.:.:|:::.|
Human   173 KMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMK--------------------------- 210

  Fly   231 LIHFVVKDSIFDTAKHMPFEKQASNPNYENESIYTTIKRIVNLIKRYKYQMSYYEGSGEGNNNSD 295
             |....|:|     |:...|:...|.|:..|.:...|..|:.::     |::.::.....:.:::
Human   211 -IFVTTKNS-----KNQGIEEALKNRNFTVEKMSAEINEIIRVL-----QLTSWDEDAWASKDTE 264

  Fly   296 AY---FSFLDDDL-----WKSEKNLPSAAGG-----------------GRMVNNTNFRK------ 329
            |.   .:.:|..|     |..:   |||:.|                 |.:......|:
Human   265 AMKRALASIDSKLNQAKGWLRD---PSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCK 326

  Fly   330 ---HLSSESLDLR---------------------EELSLAFEKLFEKAHDFTDSPY-------IS 363
               .::.:..|||                     :.|:...|....|....|:|..       .:
Human   327 MLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAA 391

  Fly   364 HN--------------------HRKNILSYCDNCKLEFDLY--------LNTIMKDQHEGGGG-- 398
            .|                    ..:.|...||:.|...|:.        |.:.:.|....|.|
Human   392 QNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDS 456

  Fly   399 -------------------------------------AGGV--------------GGVGGGQVKG 412
                                                 .|.:              .|||...::|
Human   457 PEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRG 521

  Fly   413 ----------------RSDPELGMLSSLEELCKQL-------------VVSVSSQIEDFNESLKI 448
                            |.| .|.....:::|..||             ..:::||::|..:.||.
Human   522 LVAEGHRLANVMMGPYRQD-LLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKA 585

  Fly   449 ----------------CSKLVKSFHVLAT-NYDIDN----LNQRSSKFHDCCDHIFDICKLLQHI 492
                            .:..:|...|.|| ..|..|    .::|::.|.   :|...:....:..
Human   586 RMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFE---NHSGKLGATAEKA 647

  Fly   493 A--------PTERLQVQAKCLAINLRIYGPQVFIAAKILWKYSNSSAANENFESFSDMW------ 543
            |        ..|.:|...|    ..|...|||..||:||.:...:.||.|:||:..:.|
Human   648 AAVGTANKSTVEGIQASVK----TARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEK 708

  Fly   544 --------------------------------------KWLTNEISMIAKK----ILVSINTTKG 566
                                                  :.|....:.||::    :||:....:.
Human   709 MTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVEN 773

  Fly   567 DRDAD-AEKCESSSDKLTKS--------------------------------------------- 585
            ..|.. .|..:::||:|:|:
Human   774 SEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQ 838

  Fly   586 ----------------------------------------------------------------- 585

Human   839 EPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAAR 903

  Fly   586 -------------GVPATEDG---------------------------EMVEYSGLEGKNNGDLN 610
                         |:||.|.|                           |::.....:..|...|.
Human   904 QLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILA 968

  Fly   611 TYQS------KWSEDLSDDNDILKRAKNMSAMAFLMYQFTKGNGSLRTTQDLFTQAEYFAEEANR 669
            ..||      |||   |..|||:..||.|:.:...|.:..:|...  |.:.|...|:..|:.::.
Human   969 AAQSLHREATKWS---SKGNDIIAAAKRMALLMAEMSRLVRGGSG--TKRALIQCAKDIAKASDE 1028

  Fly   670 LYKVLRHFSYQVPASDNKKDLLNILDRVPTFVQALQF--TVKDHTVGKA---------ATFVKVD 723
            :.::.:..:.|......:.:||.:.:|:||....|:.  |||...:|:.         ||    :
Human  1029 VTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQAT----E 1089

  Fly   724 HVIRETKNLMNVINKVVSKCFECATKYKLNLTGIT 758
            .::...:|||..:.:.|.:....:.|.:.: .|.|
Human  1090 MLVHNAQNLMQSVKETVREAEAASIKIRTD-AGFT 1123

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Catr	NP_726389.4	Vinculin	52..755	CDD:279395	167/1121 (15%)
VCL	NP_054706.1	N-terminal globular head. /evidence=ECO:0000269\|PubMed:20484056	1..835		126/840 (15%)
		Vinculin	66..1134	CDD:395830	169/1124 (15%)
		Talin-interaction. /evidence=ECO:0000250\|UniProtKB:P12003	168..208		4/39 (10%)
		3 X 112 AA tandem repeats. /evidence=ECO:0000255	259..589		44/333 (13%)
		Interaction with ACTN4. /evidence=ECO:0000269\|PubMed:15988023, ECO:0007744\|PDB:1YDI	741..764		3/22 (14%)
		Linker (Pro-rich). /evidence=ECO:0000269\|PubMed:20484056	836..878		0/41 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	857..887		0/29 (0%)
		C-terminal tail. /evidence=ECO:0000269\|PubMed:20484056	879..1134		46/255 (18%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250\|UniProtKB:Q64727	1003..1046		6/44 (14%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250\|UniProtKB:Q64727	1120..1134		2/4 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3681
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810