Protein Alignment RyR and itpr3

DIOPT Version :10

• Sequence 1: NP_001246211.1
• Gene: RyR / 49090
• FlyBaseID: FBgn0011286
• Length: 5134
• Species: Drosophila melanogaster
• Sequence 2: XP_017212869.1
• Gene: itpr3 / 794666
• ZFINID: ZDB-GENE-070605-1
• Length: 2635
• Species: Danio rerio
• Alignment Length: 3672
• Identity: 634/3672 - (17%)
• Similarity: 1147/3672 - (31%)
• Gaps: 1441/3672 - (39%)

  Fly  1736 VSMLALHIPEEDRCI------DILELIEMDKLLSFHAHSLTLYAALCYQSNYRAAHALCQHVDQK 1794
            :|.|.|   .:|||:      |:....:..:...|..:.:..|:|                  ||
Zfish    27 ISTLGL---VDDRCVVEPASGDLENPPKKFRDCLFKVYPMNRYSA------------------QK 70

  Fly  1795 QLLYAIRSEYMSGPLRQGFYDLLIALHLESHATTMEVCKNEYITPLGAELKELYSDEEMQHSLRS 1859
            |...|.::::....:.    |:::...|: ||..:|..:|:      ||.|:::.|.....|:..
Zfish    71 QFWKAKQAKHEKDKIG----DMVLLQKLQ-HAANLEQKQND------AENKKVHGDVVKYGSVIQ 124

  Fly  1860 LVTESVRPQLRMTEITPPVIATSSMPSVSSEPIPDIDQLY-SPKFPLEVVRQFVMEALKDAVEIN 1923
            |:.......|.:.:..|.::..::|.........:...|: .|.:.|......|:  :.|.|.:|
Zfish   125 LLHMKSNKYLTVNKRLPALLEKNAMRVTLDGTGNEGSWLFIQPFWKLRANGDNVV--VGDKVILN 187

  Fly  1924 QVHNRDPIGWTNENLFLPLIKLTDRLLLVGVLTDEDVQRLLVMIDPETWDQAFEREGKDEHRKG- 1987
            .|:...|:..::..  ||.......:..|...|...:...::..|           .|||..||
Zfish   188 PVNAGQPLHASSYE--LPDHSGCKEINSVNSNTSWKISLFMMFSD-----------HKDEVLKGG 239

  Fly  1988 -LLTMKMAEGAKLQMC----YLLHHLYDTQLRHRVESIIAFSHDFVGDLQT-------------D 2034
             ::.:..||..|...|    ..||....|.||....|  |.|.:.:.:::.             :
Zfish   240 DVVRLFHAEQEKFLTCDEYKSKLHVFLRTTLRQSATS--ATSSNALWEVEVVHHDPCRGGAGHWN 302

  Fly  2035 QLRRYIEIKQSDLPSAVAAKKTKEFRCPPREQMNQILCFKNLEPDDQDNCTCGLELRGRLGDFHD 2099
            .|.|:   |.....:.:||::...:    :|..|.|      |.|..       .::..||
Zfish   303 SLYRF---KHLATGNYLAAEENPGY----KENSNDI------EVDSS-------RIKRSLG---- 343

  Fly  2100 SLMQKVSLNALQEPDGVEGTAIEEVKTGPITKIYNFI-------------NTVKELEEGPKEVEE 2151
               :::....:..|.|.:..::.|:....:.|..:|:             ||..:....|.:::|
Zfish   344 ---ERIKYKLVAVPHGNDIASLFELDPTTLQKTDSFVPRNSYVRFRHLCTNTWIQSTNIPIDIDE 405

  Fly  2152 PE----------KKTPEEVFRKVLIKTI----VSWAEESQIENPKLVREMFSLLLRQYDTVGELV 2202
            ..          .|..:|.|..|.:..:    :.:|.::.:        |.|.::.::..  ..:
Zfish   406 ERPIRLMLGTCPTKEDKEAFAIVSVPVMEIRDLDFANDASL--------MLSTVVEKFKY--GFL 460

  Fly  2203 RALEKTYVINTRARDDVAEMWVG-LSQIRALLPVQM---SQEEEELMRKRLWKLVNNATFFQHPD 2263
            ...::.|.|  :..:||....|. |:..:..|.|.|   ::|.::|||:
Zfish   461 SPNDRRYAI--KLLEDVVFFVVDQLNNGQPALEVSMNKPNRERQKLMRE---------------- 507

  Fly  2264 LIRILRVHENVMAVMMNTLGRRAQAQSDAP---TQSEVAEGAPSKEKDTSHE-MVVACCRFLCYF 2324
                    :|::..:...:....:.:.|.|   |..|:     |.:|...:: |:..|.|.|.:.
Zfish   508 --------QNILKQIFGIIKAPFKERGDDPPLLTLEEL-----SDQKYLLYQYMLRLCYRVLRHS 559

  Fly  2325 CRTGRQNQKAMFDHFDFLLDNANILLARPSLRGSTPLDVAYSSLMENTELALALREH-YLEKIAV 2388
            ....|:||:.:...|                 |.....:.|..|.|:|..||..... .|||
Zfish   560 QEDYRKNQEYIAKQF-----------------GMMQSQIGYDILAEDTITALLHNNRKLLEK--- 604

  Fly  2389 YLSRCGLQSNSELVEKGYPDLGWDPVEGERYLDFLRYCVWVNGESVEENANLVIRLLI--RRPEC 2451
            ::::..:::...||.|..     :|    |:||:|......|..::.....|:.:.::  :..:.
Zfish   605 HITKTEVETFVSLVRKNR-----EP----RFLDYLSDLCVSNNVAIPVTQELICKCVLNAKNQDI 660

  Fly  2452 LGPALRGEGEGLFRAIVEANRMSERISD-----------------------RCKMQDEAEGTIAG 2493
            |....|       ||..|:...||.:.:                       |...|:..:|.:
Zfish   661 LMKTER-------RAAKESQHTSEYLEEFGDEDEVWLIWSEKENENHEKSIRQLAQEARQGNV-- 716

  Fly  2494 LNFTHPLPEGEEDEDYIDTGAAILNFYCTLVDLLGRCAPDAS-------------------VIEQ 2539
                       .||:       :|.:|...:.|..|...|..                   ::::
Zfish   717 -----------HDEN-------VLTYYRYQLKLFARMCFDRQYLAIDVISKQLDIELISLCMMDE 763

  Fly  2540 GKNESLRA---RAILRSLVPLEDLQGVLSLKFTLSQTAPGEEKPKS----DMPSGL--LPNNKQ- 2594
            .....|||   |.:|.:.|..:..:.|..:||....|    |.|.|    |..|.|  ..:||:
Zfish   764 TLPFDLRASFCRLMLHAHVDRDPQELVTPIKFARLWT----EIPTSISIKDYDSHLDYSRDNKKN 824

  Fly  2595 ---SIVLFLERVY-----------GIEAQDLFYRLLEDA----------FLPDLR-TATILDKSD 2634
               :.:.|:|...           ..|...|.|.::..|          |...|| |.|:|...|
Zfish   825 KFANTMAFMEEYLNNVLIDDLPFANEEKNKLTYEVVSLARHLIYFGFYSFFELLRLTRTLLGIID 889

  Fly  2635 GSESDMALAMNRYIGNSILPLLIKHSKFYNEAENYASLLDATLHTVYRLSKNRMLTK-------- 2691
            .:.|:.          ||.||       :|: :.....:..::|.:.::....:|.:
Zfish   890 CTPSNA----------SINPL-------FND-DGSGKNVRRSIHGMGQMMSTMVLNRKPSLFSAP 936

  Fly  2692 --------GQREAVS-----------------DFLVALTSQMQPAMLLKLLRKLTVDVSKLSEYT 2731
                    |.::::.                 .|::::....:.:.||.:.:|..|||..:.:..
Zfish   937 GRTGDSQVGSKDSIDTQDITVMDTKLKILEILQFILSVRLDYRLSFLLSVFKKEFVDVYPMEDAD 1001

  Fly  2732 TVALRLLTLHFDRCA----KYYGSTQGQGSYGAS--------SDEEKRLTMLLFSNIFDSLSNMD 2784
            .      |.|.:..|    ::.|. |.:..:|..        .||..|    :|..:...|...|
Zfish  1002 A------THHTEHEASINLQHIGE-QAEAMFGIGKGNSILEVDDEGGR----MFLRVLIHLIMHD 1055

  Fly  2785 YDPELFGKALPCLIAIGCALPPDYSLSKNTDEDYYGRQ--MGAPDQPQYMPNPIDTNNV-HLDND 2846
            |.|.:.| ||..|.                 :.:..||  :....|.|.:.:..|.:|. |:..|
Zfish  1056 YPPLVSG-ALQLLF-----------------KHFSQRQEVLHTFKQVQLLISTQDVDNYKHIKRD 1102

  Fly  2847 LN---SLVQKFSEHYHDAWASRRLEGGWTYGDIRSD-NDRKHPRLKPYNMLSEYERERYRDPVRE 2907
            |:   ::|:|     .:.|..::...|   ||.:.| .|:|.|.
Zfish  1103 LDLLRTMVEK-----SELWVIKKSSSG---GDGKKDKKDKKEPE--------------------- 1138

  Fly  2908 CLKGLLAIGWTVEHSEVEVALNHRGSTRRQSKPQINE-FQNEGSPFNYNPHPVDMSNLTLSREMQ 2971
                             .|:......:::|:..:.|| :||                      ::
Zfish  1139 -----------------AVSPEEEADSKKQTTEKSNESYQN----------------------VK 1164

  Fly  2972 NMAERLAENSHDIWAKKKNEELNGCGGVIHPQLVPYDLLTDKEKKKDRERSQEFLKYMQYQGYKL 3036
            .:.|||.:.......||:...|...|.    ..|..|||.....:.|    .:.|:.::|....|
Zfish  1165 EILERLNKMCSSGVFKKQQRLLKNMGA----HKVMLDLLQISYDRND----TKMLEIIKYTHLFL 1221

  Fly  3037 HKPSKGGAVEEGGATQAAVELRFSYSLLEKLIQYLDRATINMKLLKPSTTFSRRSSFKTATRDIK 3101
            .|...|....:              :||.|.:...    :..:||:..|
Zfish  1222 QKFCTGNLENQ--------------ALLHKHLNLF----LTPRLLEAET---------------- 1252

  Fly  3102 FFSKVVLPLMEKYFSTHRNYFIAIATATNNIGAAS-LKEKEMVASIFCKLAALLRNRLSAFGPDV 3165
                     |::.||             ||....| :.|..:...|.|         |:..|..:
Zfish  1253 ---------MQQIFS-------------NNFQLCSEISESVLHHFIHC---------LATQGRHI 1286

  Fly  3166 RITVRCLQVLVKGIDARTLTKNCPEFIRTSMLTFFNQTSDDLGNTILNLQDGKYSHLRGTHLKTS 3230
            :. :..|..::|.  .....|.|.:.|.|.:    ....||:  .:|...|..::.:  ..|.|.
Zfish  1287 QY-LNFLHTIIKA--EGKYVKKCQDMIMTEL----TSAGDDV--VVLYTDDTSFNTM--VELMTQ 1340

  Fly  3231 TSLGYVNQVVLPVLTAMFDHLAACDYGSDLLLDEIQVASYKILAALYHLGTDGTLTHDRKYLKTE 3295
            :..|..:...|....::.:.||||..|.: :..||:..|...|..:..:     :||:....:.:
Zfish  1341 SREGVKDDSPLRYHISLVELLAACAEGKN-VYTEIKCTSLLPLEEMVKV-----ITHEDCITEVK 1399

  Fly  3296 IERHRPALGSCLGAYSSCFPVAFLEPHLNKHNQYSLLNRIADHSLEA-QDIMVKMESCMPNLETI 3359
            |            ||.:.....:::..:.....|:     ::|..:. ::..|.|.....|.|..
Zfish  1400 I------------AYVNFVNHCYVDTEVEMKEIYT-----SNHIWKLFENFTVDMARVCSNREKR 1447

  Fly  3360 LAEVDQFVESDKTYNDAPHIIDVILPLLCAYL--PFWWSQGPDNVSPTSGNHVTMVTADHMNPLL 3422
            ::  |..:|.        ::|.|:|..:.|:.  ||     .:|.:.|..:|.|      :..||
Zfish  1448 MS--DPVLEK--------YVIQVVLDTVTAFFSSPF-----SENSTSTEAHHTT------VKQLL 1491

  Fly  3423 RNVLKMIKKNIGNDNAPWMTRIAAYTQQIIINTSEELLKDPFLPLAERVKKRTENMLHKEDSMRG 3487
            ::.::::       :.||:........:..|.|.....|...:||...::.....||...:|:
Zfish  1492 QSTMRLL-------DCPWLQPQQKVQVESCIRTLAVTTKSRSIPLPVELEAHVNMMLSHSNSL-- 1547

  Fly  3488 FIKSATDDTSQVETQLQEDWNLLVRDIYSFYPLLIKYVDLQRNHWLKDNIPEAEELYNHVAEIFN 3552
                    |....:...:..:.|.|..            ...|.|...||.|      .:.:|.|
Zfish  1548 --------TLSRSSHSNKSMSRLTRPA------------APTNPWDYKNIIE------KLQDIIN 1586

  Fly  3553 IWSKSQYFLKEEQNFISANEIDNMALIMPTATRRSAISEGAPAVGGKVKKKKKNRDKKRDKDKEV 3617
            ...:....|...:..:..:.:....|:....|...:..|.    ||.:.|
Zfish  1587 TLEERVMPLVNAELSVLVDVLHQPELLFLEGTDARSRCES----GGFISK--------------- 1632

  Fly  3618 QASLMVACLKRLLPVGLNLFAGREQELVQHCKDRYLKKMPEYDVIEFARNQLTLPDKLDPSDEMS 3682
                                      |:||
Zfish  1633 --------------------------LIQH----------------------------------- 1636

  Fly  3683 WQHYLYSKLGKTEEPVDEQALEKANVNSNEKGKDKTQETVDRIVAMAKVLFGLHMAASSKNRSKR 3747
                                 .||.:||:||             ...|||..|
Zfish  1637 ---------------------TKALMNSDEK-------------LCIKVLRTL------------ 1655

  Fly  3748 WGSKISVARRQAVISSLRAKHLYRMSRHRACNIFARSYYEQWLQEENVGQEVMVEDLTQTFEDSE 3812
                                                             ||:::.:|
Zfish  1656 -------------------------------------------------QEMLIREL-------- 1663

  Fly  3813 KSKKEGEETDSKPDPLTQLVTTFCRGAMTERSGALQEDLLYMSYAQIAAKSTGKEEEEGGDEEGG 3877
                   :.|.|...|.::               |.::.||.:...|.|:..    |..|..||
Zfish  1664 -------DFDEKGFALRKV---------------LLQNYLYNNKKNIKAELV----EHAGGAEG- 1701

  Fly  3878 EGGEEGEGTSIHEQEMEKQKLLFHQARLSNRGVAEMVLLHISASKGIPSEMVMTTLNLGIAILRG 3942
                          |.:...:...|.||...|..::....|:::|.  .::...::.|.|.:|.|
Zfish  1702 --------------ERDWLTVAALQCRLDKEGGTKLFTDLITSTKN--EKIFQESIQLAICLLEG 1750

  Fly  3943 GNIDIQMG----MLNHLKEKKDVGFFTSIAGLMNSC-----SVLDLDAFERNTKAEEYIPSAGAG 3998
            ||.:||..    |:...|.:|   ||..:...|.:.     |.:.::..|.:.||::     ...
Zfish  1751 GNTEIQNSFYKLMMGDNKSEK---FFKVLNDRMKNAQLDIKSTVSVNVGEMSNKAKD-----DKD 1807

  Fly  3999 LGVGSEGAAG------EKNMHDAEFTCA------LFRFIQLTCEGHNLEWQNYLRTQAGNTTTVN 4051
            |..|...:.|      |:...:.|...:      :.||:||.||.||.:.||:||.| .|.|..|
Zfish  1808 LETGGVSSFGQPEPQPEQQEVETEMGPSVTIMKPILRFLQLLCENHNQDLQNFLRIQ-NNKTNYN 1871

  Fly  4052 VVICTVDYLLRLQES------IMDFYWHYSSKEIIDPAGKANFFKAIEVASQVFNTLTEVIQGPC 4110
            :|..|:.:|..:..|      ::..|.:.::.|:|               :|...||||..||||
Zfish  1872 LVSETLQFLDIMCGSTTGGLGLLGLYINENNVELI---------------TQTLETLTEYCQGPC 1921

  Fly  4111 TLNQQALAHSRLWDAVGGFLFLFSHMQDKLSKHSSQVDLLKEL----------------LNLQKD 4159
            ..||..:                      :|...:.:|::..|                |.|:.:
Zfish  1922 QENQTCI----------------------VSHECNGIDIITALILNDISPLCRYRMELVLQLKDN 1964

  Fly  4160 MITMMLSMLEGNVVNGTIGKQMVDTLVESASNVELILKYFDMF-LKLADLIESPSFHEVDMKNEG 4223
            ...::|:::|..               ..:.|.|.||  |::. .:|.:||:.....|.:.| ||
Zfish  1965 ASKLLLALMESR---------------HDSENAERIL--FNLRPRELVELIKKAYLQEGECK-EG 2011

  Fly  4224 WVTPKDFREKMEQSKNYTPEEMDFLLACCERNHEGKIDYRAFVEHFHEPSKEIGFNLAVLLTNLS 4288
            .|:|                                              :|:|.|:.:|...|:
Zfish  2012 EVSP----------------------------------------------REVGHNIYILALQLA 2030

  Fly  4289 EHMPNEPRLARFLETAGSVLNYFEPFLGRIEILGSSKRIERVYFEIKDSNIEQWEKPQIRESKRA 4353
            .|              ..||         :.:|...|:|:
Zfish  2031 RH--------------NKVL---------LTLLKPVKKIK------------------------- 2047

  Fly  4354 FFYSIVTEGGDKEKLEAFVNFCEDAIFEMTHASGLMATDDGGGNVKRDTAYSSYMSEEEEERAAR 4418
                                                                    |||||..:.
Zfish  2048 --------------------------------------------------------EEEEESISS 2056

  Fly  4419 DPIRRTITAVKEGLKFGVHMLSPANIKHQIGVMQTKSIPELIVGFFKIIFYIFYYTGYAHFCVVR 4483
                                                                             
Zfish  2057 ----------------------------------------------------------------- 2056

  Fly  4484 YIFGILLNLMRGPAPEQEEEPVVEEETFGRALPPLPLEEPPGTVQAFGLDINKEENGMYKVVVHE 4548
                 :|||.:    :||||.....|.:.|              |...::|.:|:..|.::|...
Zfish  2057 -----MLNLNK----QQEEEKEDPLEHYDR--------------QTAQIEIVREDRSMEQIVFPV 2098

  Fly  4549 SPANSSMEEGGESSPEDGAAASGELVEGEPHQEPISIVDLLGGEAAKK---AAQERQEAQKA--- 4607
            .|                                  |.:.|..|:..:   ..::.::..|.
Zfish  2099 HP----------------------------------ICEFLTEESKFRVFTTTEQDEQGSKVTNF 2129

  Fly  4608 -QEAAMASIEAE-AKKSSSAPQETPAVHQIDFSQYTHRAVSFLARNFYNLK-YVALVLAFSINFM 4669
             ::.:....|.| .||..|.|          ...:..|.:|......:||. ::.|::|
Zfish  2130 FEQTSFLHNEMEWQKKLRSMP----------VLYWFSRRMSLWGTISFNLAVFINLIIA------ 2178

  Fly  4670 LLFYKVTS-FTEEADSSAEEELILGSGSGGGADITGSGFGGSGDGGSGDGEMEDEIPELVHVDED 4733
             |||...| .:...|||.   |::|.....|..:.|..|...|
Zfish  2179 -LFYPHDSGHSGSIDSSL---LLMGFWCFAGLAVLGLLFKRYG---------------------- 2217

  Fly  4734 FFYMEHVLRIAACLHSLVSLAMLIAYYHLKVPLAIFKREKEIARRLEFEGLFIAEQPEDDDFKSH 4798
                         ..||.....|...||..:...:
Zfish  2218 -------------FQSLTVAITLRCIYHFGIGPTL------------------------------ 2239

  Fly  4799 WDKLVISAKSFPVNYWDKFVKKKVRQKYSETYDFDSISNLLGMEKSTFAAQESEETGIFKYIMNI 4863
               |::.|    :|..:|.|           |....:.|                .|.|  ||.
Zfish  2240 ---LLLGA----LNLINKIV-----------YLVSFVGN----------------NGTF--IMG- 2267

  Fly  4864 DWRYQVWKAGVTFTDNAFLYSLWYFSFSVMGNF-NNFFFAAHLLDVAVGFKTLRTILQSVTHNGK 4927
                  :||.|  .|..|||.:.|...|.:|.| :.||::..|.|:....:||..:::|||.||:
Zfish  2268 ------YKAMV--MDVEFLYHVAYVLTSSLGLFVHEFFYSILLFDLIYREETLFNVIKSVTRNGR 2324

  Fly  4928 QLVLTVMLLTIIVYIYTVIAFNFFRKFYIQ----------------------------------- 4957
            .::||.:|..|:||:::::.|.|.|..:|.
Zfish  2325 SILLTAVLAIILVYLFSIVGFLFLRNDFIMEVDHLASPAPGDTESFMSSCSSDGIDCTEETGLLA 2389

  Fly  4958 --EEDEE-VDKKCHDMLTCFVFHLYKGVRAGGGIGDEIGDPDGDDYEVY--RIIFDITFFFFVII 5017
              ||||: .::.|..:|.|.:..|..|:|.|||:||.:..|..:: .::  |:::|:.|:|.|||
Zfish  2390 PAEEDEDNTERACDTLLMCIITVLNHGLRNGGGVGDVLRKPSKNE-PLFPARVVYDLLFYFIVII 2453

  Fly  5018 ILLAIIQGLIIDAFGELRDQLESVKDNMESNCFICGMGKDFFDIVPHGFDTHVQKEHNLANYMFF 5082
            |:|.:|.|:|||.|.:||.:.:..::.:::.|||||:.:|.||.....|:.|::.|||:.||::|
Zfish  2454 IVLNLIFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTVSFEEHIKLEHNIWNYLYF 2518

  Fly  5083 LMHLINKPDTEYTGQETYVWNMYQQRSWDFFP 5114
            ::.:..|..|:|||.|:||..|.:.::.|:||
Zfish  2519 IVLIREKNKTDYTGPESYVALMIKNKNLDWFP 2550

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RyR	NP_001246211.1	Ins145_P3_rec	12..206	CDD:462572
		beta-trefoil_MIR_RyR	215..395	CDD:467749
		RYDR_ITPR	443..641	CDD:460175
		SPRY1_RyR	647..799	CDD:240457
		RyR	854..943	CDD:460419
		RyR	967..1056	CDD:460419
		SPRY2_RyR	1079..1213	CDD:240458
		SPRY3_RyR	1518..1669	CDD:293937
		RYDR_ITPR	2228..2457	CDD:460175	43/238 (18%)
		RyR	2831..2920	CDD:460419	15/93 (16%)
		RyR	2954..3036	CDD:460419	14/81 (17%)
		RIH_assoc	4013..4130	CDD:462482	35/128 (27%)
		RR_TM4-6	4526..4768	CDD:461918	40/251 (16%)
		Ion_trans	4856..>4979	CDD:459842	42/161 (26%)
itpr3	XP_017212869.1	Ins145_P3_rec	6..231	CDD:462572	43/250 (17%)
		beta-trefoil_MIR_ITPR3	226..435	CDD:467760	43/248 (17%)
		RYDR_ITPR	471..666	CDD:460175	47/252 (19%)
		RYDR_ITPR	1164..1310	CDD:460175	38/221 (17%)
		RIH_assoc	1834..1943	CDD:462482	36/146 (25%)
		PAP2_like	2151..>2184	CDD:469784	9/39 (23%)
		Ion_trans	<2277..2475	CDD:459842	61/198 (31%)