Protein Alignment RyR and itpr1b

DIOPT Version :10

• Sequence 1: NP_001246211.1
• Gene: RyR / 49090
• FlyBaseID: FBgn0011286
• Length: 5134
• Species: Drosophila melanogaster
• Sequence 2: XP_068080493.2
• Gene: itpr1b / 100149388
• ZFINID: ZDB-GENE-070604-2
• Length: 2819
• Species: Danio rerio
• Alignment Length: 3797
• Identity: 675/3797 - (17%)
• Similarity: 1197/3797 - (31%)
• Gaps: 1568/3797 - (41%)

  Fly  1775 LCYQSNYRAAHALCQHVDQKQLLYAIRSEYMSGPLRQGFYD--LLIALHLESHATTMEVCKNEYI 1837
            ||..:.|.|         |||...|.:      |......|  ||..||   ||..:|..:||
Zfish    60 LCPMNRYSA---------QKQFWKAAK------PGGNSTTDTVLLNKLH---HAADLEKKQNE-- 104

  Fly  1838 TPLGAELKELYSDEEMQHSLRSLVTESVRPQLRMTEITPPVIATSSMPSVSSEPIPDIDQLY-SP 1901
                :|.::|........::..|:.......|.:.:..|.::..::|.........:....| .|
Zfish   105 ----SENRKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDSAGNEGSWFYIQP 165

  Fly  1902 KFPLEVVRQFVMEALKDAVEINQVHNRDPIGWTNENL-------------------FLPLIKLTD 1947
            .:.|..:...|:  :.|.|.:|.|:...|:..::..|                   .:..:|.:|
Zfish   166 FYKLRSIGDSVV--IGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCNTSWKIVLFMKWSD 228

  Fly  1948 RLLLVGVLTDEDVQRLLVMIDPETWDQAFEREGK-----DEHRK---------GLLTMKMAEGAK 1998
            .  ...:|...||.||            |..|.:     |||||         |..:...|..:|
Zfish   229 N--KEAILKGGDVVRL------------FHAEQEKFLTCDEHRKKQHVFLRTTGRQSATSATSSK 279

  Fly  1999 LQMCYLLHHLYDTQ-LRHRV--------ESIIAFSHDFVGDLQTDQLRRYIEIKQSDLPSAVAAK 2054
            .        |::.: ::|..        .|:..|.|...|        .|:..:.|::.|..:.
Zfish   280 A--------LWEIEVVQHDPCRGGAGYWNSLFRFKHLATG--------HYLAAEVSEVCSTSSG- 327

  Fly  2055 KTKEFRCPPREQMNQILCFKNL--EPDDQDNCTCGLELRGRLGDF----------HDSLM----- 2102
              ....|      :||....:|  .||.::.   ..|.|..:.||          |::|.
Zfish   328 --SSLSC------SQISTNLSLWVNPDYEEE---SQESRSSVADFSPFNFQLDSEHEALRARLRT 381

  Fly  2103 --QKVSLNALQEPDGVEGTAIEEVKTGPITKIYNFINTVKELEEGPKEVEEPEKKTPEEVFRKVL 2165
              :||..:.:..|||                  |.|:::.||:  |..:...:...|...:.::.
Zfish   382 PNEKVMYSLVSVPDG------------------NDISSIFELD--PTTLRGGDSLVPRNSYVRLR 426

  Fly  2166 IKTIVSWAE------ESQIENPKLVR----------EMFSLL------LRQYDTVGELVRAL--- 2205
            .....:|..      :.:.|.|.::|          |.|:::      :|..|...:..:.|
Zfish   427 HLCTNTWVHSTNNPIDKEEEKPVMLRIGTSAVKEDKEAFAIVPVPPAEVRDLDFANDASKVLASI 491

  Fly  2206 ------------EKTYVINTRARDDVAEMWVGL----SQIRALLPVQMSQEEEELMR-----KRL 2249
                        |:.:|  |:..:|:....|.:    ..:..::..:.::|.::|||     |::
Zfish   492 AGKLEKGTITQNERRFV--TKLLEDLVFFVVDIPNNGQDVLEIMVNKPNRERQKLMREQNILKQI 554

  Fly  2250 WKLVNNATFFQHPD--LIRILRVHENVMAVMMNTLGRRAQAQSDAPTQSEVAEGAPSKEKDTSHE 2312
            :||: .|.|....|  ::|:..:::                |..||.:                .
Zfish   555 FKLL-QAPFTDSGDGPMLRLEELND----------------QRHAPFR----------------H 586

  Fly  2313 MVVACCRFLCYFCRTGRQNQKAMFDHFDFLLDNANILLARPSLRGSTPLDVAYSSLMENTELALA 2377
            :...|.|.|.:..:..|:||:.:...|.|:                 ...:.|..|.|:|..||.
Zfish   587 ICRLCYRVLRHSQQDYRKNQEYIAKQFRFM-----------------QKQIGYDVLAEDTITALL 634

  Fly  2378 LREH-YLEKIAVYLSRCGLQSNSELVEKGYPDLGWDPVEGERYLDFLR-YCVWVNGESV------ 2434
            .... .|||   :::...:.:...||.|..     :|    |:||:|. .||.:| :|:
Zfish   635 HNNRKLLEK---HITAAEIDTFVSLVRKNR-----EP----RFLDYLSDLCVSMN-KSIPVTQEL 686

  Fly  2435 -------EENANLVI--RLLIRRPECLGPALRGEGEGLFRAIVE----------ANRMSERISDR 2480
                   ..||:::|  :|::.|.|.      ..|||...:.::          :|:..:..|.|
Zfish   687 ICNAVLNPANADILIETKLVLSRFEV------ESGEGPMESEIDEEEVWLFWKNSNKEVKSKSIR 745

  Fly  2481 CKMQDEAEGTIAGLNFTHPLPEGEEDEDYIDTGAAILNFYCTL-----------------VDLLG 2528
            ...||..||.             ::|:|.|......||.:..:                 |||:.
Zfish   746 ELAQDAKEGQ-------------KDDQDVISYYRYQLNLFARMCLDRQYLAIEKISGQLDVDLIL 797

  Fly  2529 RCAPDASVIEQGKNESLRA---RAILRSLVPLEDLQGVLSLKFTL----------------SQTA 2574
            ||..|..:     ...|||   |.:|...|..:..:.|:.:|:..                ..|:
Zfish   798 RCMSDEDL-----PYDLRASFCRLMLHMHVDRDPQEQVMPVKYARLWSEIPSKIAIDDYDNDGTS 857

  Fly  2575 PGEEKPKSDMPSGLLPNNKQSIVL----FLER--------VYGIEAQDLFY----------RLLE 2617
            ..|.|.:..:....:.|..:.:||    |.::        |..: |::|.|          ||.:
Zfish   858 RDEIKERFALTMDFVENYLRDVVLQNVPFSDKEKNKLTFEVVNL-ARNLIYFGFYNFSDLLRLTK 921

  Fly  2618 D--AFLPDLRTATI-----LDKSDGSESDMALAMNRYIGNSILPLLIKHSKFYNEAENYASLLDA 2675
            .  |.|..:..:||     ::|.|.|:|...:.....:|..:..::::...|
Zfish   922 ILLAILDCVHVSTIYPINKMEKGDESKSSNVMKSIHGVGELMTQVVLRGGGF------------- 973

  Fly  2676 TLHTVYRLSKNRMLTKGQREAVSDFLVALTSQMQPAMLLKLLRKLTVDVSKLSEYTTVALR---- 2736
             |.|......:..:.|.|.|...:.::.:.::::...:|:.:..:.:|. ::|....:..|
Zfish   974 -LPTTPTTPPDGDVVKTQTEPEKEDILVMDTKLKIIEILQFILNVRLDY-RISCLLCIFKREFDE 1036

  Fly  2737 ---------------------LLTLHFDRCAKYYGSTQGQGSYGASSDEEKRLTM-----LLFSN 2775
                                 :..|.|:...:     |.:|.:| .|:|...|.:     ..|..
Zfish  1037 SNTQTDTAPAPNADGPNSIPNIPALDFENIEE-----QAEGIFG-GSEENTPLDLDDHGGRTFLR 1095

  Fly  2776 IFDSLSNMDYDPELFGKALPCLIAIGCALPPDYSLSKNTDEDYYGRQ--MGAPDQPQYMPNPIDT 2838
            :...|:..||.|.:.| ||..|.                 ..:..||  :.|..|.|.:....|.
Zfish  1096 VLLHLTMHDYPPLVSG-ALHLLF-----------------RHFSQRQEVLMAFKQVQLLVTSQDV 1142

  Fly  2839 NNV-HLDNDLNSL---VQKFSEHYHDAWASRRL-EGGWTYGD---IRSDNDRKHPRLKPYNMLSE 2895
            :|. .:.:||:.|   |:|     .:.|..:|. :.|...||   ..||:.:|............
Zfish  1143 DNYKQIKSDLDQLRSNVEK-----SELWVYKRQGDEGMDGGDGLPAESDHKKKRDSGSDKKKTDG 1202

  Fly  2896 YERERYRDPVRECLKGLLAIGWTVEHSEVEVALNHRGSTRRQSKPQINEFQNEGSPFNYNPHPVD 2960
            ...:.||| |:|.|..|         |::.|   ..|.:.::||.|
Zfish  1203 NGSDNYRD-VKEILLRL---------SKLCV---QEGPSGKKSKKQ------------------- 1235

  Fly  2961 MSNLTLSREMQNMAERLAENSHDIWAKKKNEELNGCGGVIHPQLVPYDLLTDKEKKKDRERSQEF 3025
                 ..|.::||      .:|.:              |:....:||:...|...::..:.:.||
Zfish  1236 -----QQRLLRNM------GAHSV--------------VLELLQIPYEKGEDVRMQEIMKLAHEF 1275

  Fly  3026 LKYM----QYQGYKLHKPSKGGAVEEGGATQAAVELRFSYSLLEKLIQYLDRATINMKLLKPSTT 3086
            |:..    |.....|||               .:.|..:..:||.:
Zfish  1276 LQNFCAGNQQNQALLHK---------------HINLFLNPGILEAI------------------- 1306

  Fly  3087 FSRRSSFKTATRDIKFFSKVVLPLMEKYFSTHRNYFIAIATATNNIGAAS-LKEKEMVASIFCKL 3150
                                          |.::.|:      ||....| :.|:.:...:.|
Zfish  1307 ------------------------------TMQHIFM------NNFQLCSEMNERVVQHFVHC-- 1333

  Fly  3151 AALLRNRLSAFGPDVRITVRCLQVLVKGIDARTLTKNCPEFIRTSM-------LTFFNQTSDDLG 3208
                   :...|..|:. ::.||.:||.  .....|.|.:.:...:       |.|:|..:.  .
Zfish  1334 -------IETHGRHVQY-LKFLQTIVKA--ENKFIKKCQDIVMAELVNSGEDVLVFYNDRAS--F 1386

  Fly  3209 NTILNLQDGKYSHLRGTHLKTSTSLGYVNQVVLPVLTAMFDHLAACDYGSD----------LLLD 3263
            .|::.:...:...:.    :.|..:.:::.|.|         ||.|..|.:          |.||
Zfish  1387 QTLVQMMRSERDRMD----ENSPLMYHIHLVEL---------LAVCTEGKNVYTEIKCNSLLPLD 1438

  Fly  3264 EI-QVASYKILAALYHLGTDGTLTHDRKYLKTEIERHRPALGSCLGAYSSCFPVAFLEPHLNKHN 3327
            :| :|.::|.......:.....|.|  .|:.||:|...        .|:|        .|:.|..
Zfish  1439 DIVRVVTHKDCIPEVKIAYINFLNH--CYVDTEVEMKE--------IYTS--------NHMWKLF 1485

  Fly  3328 QYSLLN--RIADHSLEAQDIMVKMESCMPNLETILAEVDQFVESDKTYNDAPHIIDVILPLLCAY 3390
            :..|::  |:.:::.:.:.....:||.:  .||:::.|..|..
Zfish  1486 ENFLVDICRVCNNTSDRKHADTTLESYV--TETVMSIVTTFFS---------------------- 1526

  Fly  3391 LPFWWSQGPDNVSPTSGNHVTMVTADHM------------NP----LLRNVLKMIKKNIGNDNAP 3439
                        ||.|....::..|.||            .|    ||:.|.::.       :..
Zfish  1527 ------------SPFSDQSTSLQVARHMYASILGKITETRQPVFVQLLQGVFRVY-------HCS 1572

  Fly  3440 WMTRIAAYTQQIIINTSEELLKDPFLPLAERVKKRTENMLHKEDSMRGFIKSATDDTSQVETQLQ 3504
            |:......:.:..|....::.|...:.:...:..:..|:         |:||     :.|..:..
Zfish  1573 WLLPSQKGSVESCIKVLSDVAKSRAIAIPVDLDSQVNNL---------FVKS-----NNVVQKTI 1623

  Fly  3505 EDWNLLVRDIYSFYPLLIKYVDLQRNHWLKDNIPEAEELYNHVAEIFNIWSKSQYFLKEEQNFIS 3569
            .:|.|..                 ||...:|:...|.:.|.::.|             ..|:.:|
Zfish  1624 LNWRLSA-----------------RNASRRDSTLTASKDYRNIIE-------------RLQDIVS 1658

  Fly  3570 ANE-----------------IDNMALIMP--TATRRSAISEGAPAVGGKVKKKKKNRDKKRDKDK 3615
            |.|                 :....|:.|  |..||...|      ||.:.|..|:..:..::::
Zfish  1659 ALEDRLRPLVQAELSVLVDVLHRPELLFPENTDARRKCES------GGFISKLIKHTKQLLEENE 1717

  Fly  3616 EVQASLMVACLKRLLPVGLNLFAGREQELVQHCKDR-YLKKMPEYDVIEFARNQLTLPDKLDPSD 3679
            |      ..|:|.|..:         :|::  .||| |.:|:..:|            |:||
Zfish  1718 E------RLCIKVLQTL---------REMM--TKDRGYGEKLIAFD------------DELD--- 1750

  Fly  3680 EMSWQHYLYSKLGKTEEPVDEQALEKANVNSNEKGKDKTQETVDRIVAMAKVLFGLHMAASSKNR 3744
                    .::|....||  |...|:.:.|..:|..:
Zfish  1751 --------VAELAPPPEP--EVPAEEFDPNQPQKQLE---------------------------- 1777

  Fly  3745 SKRWGSKISVARRQAVISSLRAKHLYRMSRHRACNIFARSYYEQWLQEENVGQEVMVEDLTQTFE 3809
                ..|.|.|.||.:::                     .||..:
Zfish  1778 ----DQKRSEALRQVLVN---------------------RYYGNF-------------------- 1797

  Fly  3810 DSEKSKKEGEETDSKPDPLTQLVTTFCRGAMTERSGALQEDLLYMSYAQIAAKSTGKEEEEGGDE 3874
                  |.|...||        :|.|..|.:|                     ..|:.:...|..
Zfish  1798 ------KPGGRRDS--------LTNFSNGPLT---------------------PVGQTKTPAGGG 1827

  Fly  3875 EGGEGGEEGEGTSIHEQEMEKQKLLFHQARLSNRGVAEMV--LLHISASKGIPSEMVMTTLNLGI 3937
            .||.||..|....:...|:        |..|...|.:::|  |:..:.|..|..|.::    |.|
Zfish  1828 GGGGGGSAGSRGELSLAEV--------QCHLDREGASDLVIDLIMNATSDRIFQESIL----LAI 1880

  Fly  3938 AILRGGNIDIQMGMLNHL----------------------------------------------- 3955
            |:|.|||..||......|
Zfish  1881 ALLEGGNPVIQRSFFCRLTGDNKSEKFFKVFYERMKLAQQEIKATVTVNTSDLGSKRKDEDTNDK 1945

  Fly  3956 -----KEKKDVGFFT-SIAGLMNSCSVLDLDAF---ERNTKAEEYIPSAGAGLGVGSEG--AAGE 4009
                 |:.|||...| .:...:...|.:...||   .|...|:|::.:        :||  :..|
Zfish  1946 DVQSRKKVKDVAVITEEVREQLLEASTVTKKAFSTYRREADADEHLNA--------TEGPSSTAE 2002

  Fly  4010 KNMHDAEFTC------ALFRFIQLTCEGHNLEWQNYLRTQAGNTTTVNVVICTVDYLLRLQES-- 4066
            ||..:.|.:.      .:.|.:||.||.||.|.||:||.| .|....|:|..|:.:|..:..|
Zfish  2003 KNQDENEMSVIITIMQPILRLMQLLCENHNRELQNFLRCQ-NNKNNYNLVCETLQFLDCICGSTT 2066

  Fly  4067 ----IMDFYWHYSSKEIIDPAGKANFFKAIEVASQVFNTLTEVIQGPCTLNQQALAHSRLWDAVG 4127
                ::..|.:.               |.:.:.:|...:|||..||||..||..:|
Zfish  2067 GGLGLLGLYINE---------------KNVALINQTVESLTEYCQGPCHENQNCIA--------- 2107

  Fly  4128 GFLFLFSHMQDKLSKHSSQVDLLKELLNLQKDMITMMLSMLEGNVVNGTIGKQMVDTLVESASNV 4192
                         :...:.:|::          |.::|     |.:| .:||:.:|.::|..:|.
Zfish  2108 -------------THECNGIDII----------IALIL-----NDIN-PLGKKRMDLVLELKNNA 2143

  Fly  4193 ELILKYFDMFLKLADLIESPSFHEVDMKNEGWV----TPKDFREKMEQSKNYTPEEMDFLLACCE 4253
            ..:|        || ::||    ..|.:|...:    .||:..|.::  |.|...|::     .|
Zfish  2144 SKLL--------LA-IMES----RHDSENAERILYNMRPKELVEVIK--KAYMQGEIE-----VE 2188

  Fly  4254 RNHEGKIDYRAFVEHFHEPSKEIGFNLAVLLTNLSEHMPNEPRLARFLETAGS------VLNYFE 4312
            ...:|:   ....||...| :.:|.|:.:|...|:.|   ...|...|:..|:      .|.::.
Zfish  2189 HTDDGE---NGEEEHAASP-RNVGHNIYILAHQLARH---NKELQAMLKPGGTYGEGDEALEFYA 2246

  Fly  4313 PFLGRIEILGSSKRIERVYFEIKDSNIEQWEKPQI-----RESKRAFFYSIVTEGGDK-EKLEAF 4371
            ....:|||:...:.:|::.|.:          |.|     :|||...:|:  ||..:: .|:..|
Zfish  2247 KHTAQIEIVRLDRTMEQIVFPV----------PNICEFLTQESKLRVYYT--TERDEQGSKINDF 2299

  Fly  4372 VNFCEDAIFEMTHASGLMATDDGGGNVKRDTAYSSYMSEEEEERAARDPIRRTITAVKEGLKFGV 4436
            ....||...||.....|..|.:..||
Zfish  2300 FLRSEDLFNEMNWQKKLRGTQEATGN--------------------------------------- 2325

  Fly  4437 HMLSPANIKHQIGVMQTKSIPELIVGFFKIIFYIFYYTGYAHFCVVRYIFGILLNLMRGPAPEQE 4501
                 ||           |:|
Zfish  2326 -----AN-----------SVP-------------------------------------------- 2330

  Fly  4502 EEPVVEEETFGRALPPLPLEEPPGTVQAFGLDINKEENGMYKVVVHESPANSSMEEGGESSPEDG 4566
            .:|::
Zfish  2331 SQPIL------------------------------------------------------------ 2335

  Fly  4567 AAASGELVEGEPHQEPISIVDLLGGEAAKKAAQERQEAQKAQEAAMASIEAEAKKSSSAPQETPA 4631
                                                                             
Zfish  2336 ----------------------------------------------------------------- 2335

  Fly  4632 VHQIDFSQYTHRAVSFLARNFYNLKYVA-LVLAFSINFMLLFYKVTSFTEEADSSAEEELILGSG 4695
                   .:..|.:||.:...:||..:. |::||       ||.:
Zfish  2336 -------YWCSRNMSFWSSISFNLAVLMNLLVAF-------FYPL-------------------- 2366

  Fly  4696 SGGGADITGSGFGGSGDGGSGDGEMEDEIPELVHVDEDFFYMEHVLRIAACLHSLVSLAMLIAYY 4760
            .|||  :.|             |.:|..:..|:.              ||   .|||||::|
Zfish  2367 EGGG--VRG-------------GTLEPHLSALLW--------------AA---MLVSLAIVI--- 2396

  Fly  4761 HLKVPLAIFKREKEIARRLEFEGLFIAEQPEDDDFKSHWDKLVISAKSFPVNYWDKFVKKKVRQK 4825
                                     :..||       |..:.:|::..             :|..
Zfish  2397 -------------------------VLPQP-------HGIRALIASTI-------------LRLI 2416

  Fly  4826 YSETYDFDSISNLLGMEKSTFAAQESEETGIFK------YIMNIDWRYQVWKAGVTFT------- 4877
            :|           :|:|.:.|.      .|.|.      ::|:.     |...| |||
Zfish  2417 FS-----------VGLEPTLFL------LGAFNVGNKVIFLMSF-----VGNRG-TFTRGYKAMI 2458

  Fly  4878 -DNAFLYSLWYFSFSVMGNF-NNFFFAAHLLDVAVGFKTLRTILQSVTHNGKQLVLTVMLLTIIV 4940
             |..|||.|.|.....:|.| :.||::..|.|:....:||..:::|||.||:.:|||.:|..|:|
Zfish  2459 MDVEFLYHLLYLIICSLGVFVHVFFYSLLLFDLIYREETLLNVIKSVTRNGRSIVLTAVLALILV 2523

  Fly  4941 YIYTVIAFNFFRKFYI----------------------------QEED----------------- 4960
            |:::::.:..|:..:|                            :||:
Zfish  2524 YLFSIVGYIIFKDDFILTVDRISNKTLEQSASKMGEFLAGAVCRKEENCSTETMMDAVGVQSSAL 2588

  Fly  4961 --EEVDKKCHDMLTCFVFHLYKGVRAGGGIGDEIGDPDGDD-YEVYRIIFDITFFFFVIIILLAI 5022
              |:.::.|..:|.|.|..|..|:|:|||:||.:..|..:: ....|:|:|:.|||.||||:|.:
Zfish  2589 VIEDKERTCDSLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNL 2653

  Fly  5023 IQGLIIDAFGELRDQLESVKDNMESNCFICGMGKDFFDIVPHGFDTHVQKEHNLANYMFFLMHLI 5087
            |.|:|||.|.:||.:.:..::.:::.|||||:.:|.||.....|:.|::.|||:.:|:||::.:.
Zfish  2654 IFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTVTFEEHIKVEHNMWHYLFFIVLVK 2718

  Fly  5088 NKPDTEYTGQETYVWNMYQQRSWDFFP 5114
            .|..||:||.|:||..|.:..:.|:||
Zfish  2719 VKDSTEFTGPESYVAEMIRDHNLDWFP 2745

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RyR	NP_001246211.1	Ins145_P3_rec	12..206	CDD:462572
		beta-trefoil_MIR_RyR	215..395	CDD:467749
		RYDR_ITPR	443..641	CDD:460175
		SPRY1_RyR	647..799	CDD:240457
		RyR	854..943	CDD:460419
		RyR	967..1056	CDD:460419
		SPRY2_RyR	1079..1213	CDD:240458
		SPRY3_RyR	1518..1669	CDD:293937
		RYDR_ITPR	2228..2457	CDD:460175	49/252 (19%)
		RyR	2831..2920	CDD:460419	22/96 (23%)
		RyR	2954..3036	CDD:460419	12/85 (14%)
		RIH_assoc	4013..4130	CDD:462482	33/128 (26%)
		RR_TM4-6	4526..4768	CDD:461918	25/242 (10%)
		Ion_trans	4856..>4979	CDD:459842	42/184 (23%)
itpr1b	XP_068080493.2	None