DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mbs and PPP1R12A

DIOPT Version :9

Sequence 1:	NP_001246786.1	Gene:	Mbs / 49070	FlyBaseID:	FBgn0005536	Length:	1273	Species:	Drosophila melanogaster
Sequence 2:	NP_001137357.1	Gene:	PPP1R12A / 4659	HGNCID:	7618	Length:	1030	Species:	Homo sapiens

Alignment Length:	1416	Identity:	392/1416 - (27%)
Similarity:	553/1416 - (39%)	Gaps:	544/1416 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 AMMKRAEQLKRWEESDTNRAAPTPRHEHGRRIKFSSGCVFLAACLSGDKDEVVQLLDQGADINTA 75
            |..||.||||||..|:|:...|..:.:. .::||..|.||||||.|||.|||::||.:|||||.|
Human     6 AKQKRNEQLKRWIGSETDLEPPVVKRQK-TKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYA 69

  Fly    76 NVDGLTALHQACIDDNLDMVEFLVERGADINRQDNEGWTPLHATASCGFVSIARYLVENGADVAA 140
            ||||||||||||||||:|||:||||.||:||:.|||||.||||.||||::.||.:|:..||.|.|
Human    70 NVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGA 134

  Fly   141 VNSDGDLALDLAIDVQHMAMIDYMEKMVQELNINVDEARKAEELAMLNDAKKWLRSDAAEVDRPH 205
            |||:||..||:|   :..||.:.::..|....::::.|||.||..||.||::||.|.... |..|
Human   135 VNSEGDTPLDIA---EEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHIN-DVRH 195

  Fly   206 PKTGATALHVAAAKGYTKVLGLLLAGRGNVDRQDNDGWTPLHAASHWGQRETAEMLVESLADMDI 270
            .|:|.||||||||||||:||.||:....:|:.:|.|||||||||:|||:.|...:||::|.||::
Human   196 AKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEM 260

  Fly   271 RNYAGQSCIDVADRKIVKFLEELR-------ANKRNKRRPSSQIRISDAMENH------------ 316
            .|..||:..||||..|:.:||||:       :.||:|:.|  .|..:..|:|:
Human   261 VNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSP--LIESTANMDNNQSQKTFKNKETL 323

  Fly   317 ---VEKTPTKLVRV------------------------------EVRTDATK------------- 335
               .||..:::..:                              |..||.||
Human   324 IIEPEKNASRIESLEQEKVDEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPLASVTNANTSST 388

  Fly   336 ----------------------------DAENVKPNQQIHAVEHPVEEEAPWRRKLPRTPSDSPT 372
                                        .|..:.|.::    |...|..|.||..|.:|.|....
Human   389 QAAPVAVTTPTVSSGQATPTSPIKKFPTTATKISPKEE----ERKDESPATWRLGLRKTGSYGAL 449

  Fly   373 NNQVPDRELGTNSSETA----NDVILRRTQSFENDQKFYQKYNELRARIKANSCPILPANANANA 433
            ......:| |....:||    :....|.:.|.:|.    :|..:.:....|...|.:|....:.:
Human   450 AEITASKE-GQKEKDTAGVTRSASSPRLSSSLDNK----EKEKDSKGTRLAYVAPTIPRRLASTS 509

  Fly   434 AAANKSNNNN--------------------NLSSN---NYHNNNNNNNKSDLLLGYAAGTTTTTS 475
            ....|.|.::                    |.|.|   .||.:.:...:.|.|:  ::...:|||
Human   510 DIEEKENRDSSSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHKSCSFGRRQDDLI--SSSVPSTTS 572

  Fly   476 TPTTTT------TTTSSTFNNTHSNTNNTSTTQQQPAAAAAASAANQLYSVQRSASLKDNSMYYR 534
            |||.|:      :..|||...|...|.::|       |...:|.:|:|::...:...||:
Human   573 TPTVTSAAGLQKSLLSSTSTTTKITTGSSS-------AGTQSSTSNRLWAEDSTEKEKDS----- 625

  Fly   535 KPT-VTIATSTAATAIN-SPSTTVQTPPIRSQVTAKIAEKSNNGSSSTASVSDAESSKPPPKQSA 597
            .|| |||  ..|.|.:| :.|||..|......|            |||..|.:..
Human   626 VPTAVTI--PVAPTVVNAAASTTTLTTTTAGTV------------SSTTEVRERR---------- 666

  Fly   598 SNMIKNFFKSFVPPVRDEESETQRKAHAKRVRETRRSTQGVTLDEIKSAEELVKKKNMGMANNNN 662
                    :|::.||||||||:||||.:::.|::|||||||||.:::.||:.:.:..
Human   667 --------RSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSR-------- 715

  Fly   663 NNNISTTTTNTISNSGNETSSASSTSAPTPSILGSNEQQDELQPPPPPTTPPPAVIPTTTTATDE 727
                ||.|                           .||::|                       |
Human   716 ----STRT---------------------------REQENE-----------------------E 726

  Fly   728 TEIEEVVSSPPAGQSQNDTTASFTLSAPVRRSLSTEGEADANSDPEGDQDQTVVSASYTIMPRRE 792
            .|.||
Human   727 KEKEE------------------------------------------------------------ 731

  Fly   793 RLPESSENVESIRSPQKTEPVAKSSSEDKSEEPPHARPTDLPLIPAPAAPSTEAIKSSSAATTPA 857
                                 .:...::|.||
Human   732 ---------------------KEKQDKEKQEE--------------------------------- 742

  Fly   858 ALESPVRLRDKRGLSGSQESETKSDTASPVSSHPDFNARDSLLSLYARRTTDSSAGGGGVGGGAG 922
                            .:||||..:              |.....|:|
Human   743 ----------------KKESETSRE--------------DEYKQKYSR----------------- 760

  Fly   923 VGAGGASGAATSSSTSAAERRPSWRLKFDAGSKFKLEDITSGGTYPPNNSTIIPSAPAVMAAANL 987
                                                   |...||
Human   761 ---------------------------------------TYDETY-------------------- 766

  Fly   988 STTTGVQRRISSGPIALNASNQSLNSVGRPVSAPSEGTNNSAYVTPSARKFETNATSTGATTAAT 1052
                  ||.                   ||||..|..|.:|:..|.|:..:            |:
Human   767 ------QRY-------------------RPVSTSSSTTPSSSLSTMSSSLY------------AS 794

  Fly  1053 TTSNSTSNSVSATSANHTTATAPNATSNHDDKDNDKENDNRTQ-TVIQRRRKP--KRRSTGVVH- 1113
            :..|..::.|..|||.....|..|.... :.::.:||.::::| ..|:.||:|  |||||||..
Human   795 SQLNRPNSLVGITSAYSRGITKENEREG-EKREEEKEGEDKSQPKSIRERRRPREKRRSTGVSFW 858

  Fly  1114 -IDMDELDPERQNESSNNDNEEKEKESGSERTSR-------------SRLGSTASTATTSESKSS 1164
             .|.||.:.|:|:::....|   :||:.::..||             |.||.:.|.:...|.|..
Human   859 TQDSDENEQEQQSDTEEGSN---KKETQTDSISRYETSSTSAGDRYDSLLGRSGSYSYLEERKPY 920

  Fly  1165 SSNDKTENGDGIDYKALWEAEKLENDKLRQMLKQKDDEAVQTRATL-------ERFANATTKNSL 1222
            ||  :.|..|..|:|.|:|....||:||:..|...:.|....:..|       ||||:    .||
Human   921 SS--RLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFAD----RSL 979

  Fly  1223 SELEKRERRAMERKLSELEEELKQLDAYKSDNHRLKEENAALIRVISKLSK 1273
            .|:|||||||:||::||:|||||.|...|:||.|||:||.|||||||||||
Human   980 LEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK 1030

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mbs	NP_001246786.1	ANK	50..164	CDD:238125	76/113 (67%)
		Ank_2	53..142	CDD:289560	63/88 (72%)
		ANK repeat	78..109	CDD:293786	25/30 (83%)
		ANK	106..290	CDD:238125	92/183 (50%)
		ANK repeat	111..142	CDD:293786	18/30 (60%)
		ANK repeat	209..239	CDD:293786	18/29 (62%)
		Ank_2	213..300	CDD:289560	48/93 (52%)
		ANK repeat	241..272	CDD:293786	18/30 (60%)
		PRKG1_interact	1177..1272	CDD:292521	51/101 (50%)
PPP1R12A	NP_001137357.1	KVKF motif	35..38		0/2 (0%)
		ANK 1	39..68		19/28 (68%)
		Ank_2	47..136	CDD:289560	63/88 (72%)
		ANK	67..252	CDD:238125	107/188 (57%)
		ANK repeat	72..103	CDD:293786	25/30 (83%)
		ANK 2	72..101		24/28 (86%)
		ANK repeat	105..136	CDD:293786	18/30 (60%)
		ANK 3	105..134		17/28 (61%)
		ANK repeat	138..229	CDD:293786	41/94 (44%)
		ANK 4	138..164		8/28 (29%)
		ANK 5	198..227		18/28 (64%)
		Ank_4	199..252	CDD:290365	32/52 (62%)
		ANK	226..>301	CDD:238125	35/76 (46%)
		ANK repeat	231..262	CDD:293786	18/30 (60%)
		ANK 6	231..260		18/28 (64%)
		Ank_4	232..280	CDD:290365	25/47 (53%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	290..628		63/362 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	643..928		109/639 (17%)
		Interaction with ROCK2. /evidence=ECO:0000269\|PubMed:19131646	682..864		72/501 (14%)
		PRKG1_interact	931..1029	CDD:292521	51/101 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	98	1.000	Domainoid score	I7171
eggNOG	1	0.900	-	-		E2759_KOG0505
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	363	1.000	Inparanoid score	I2180
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D225308at33208
OrthoFinder	1	1.000	-	-		FOG0002673
OrthoInspector	1	1.000	-	-		otm41111
orthoMCL	1	0.900	-	-		OOG6_105882
Panther	1	1.100	-	-	LDO	PTHR24179
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2914
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.830