DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mbs and ppp1r12a

DIOPT Version :9

Sequence 1:	NP_001246786.1	Gene:	Mbs / 49070	FlyBaseID:	FBgn0005536	Length:	1273	Species:	Drosophila melanogaster
Sequence 2:	XP_021330670.1	Gene:	ppp1r12a / 445393	ZFINID:	ZDB-GENE-041011-3	Length:	1097	Species:	Danio rerio

Alignment Length:	1445	Identity:	380/1445 - (26%)
Similarity:	582/1445 - (40%)	Gaps:	545/1445 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 AMMKRAEQLKRWEESDTNRAAPTPRHEHGRRIKFSSGCVFLAACLSGDKDEVVQLLDQGADINTA 75
            |..||.||||||..|:|:...|..:.:. .::||..|.||||||.|||.:||:::||:|||||.|
Zfish     6 AKQKRNEQLKRWIGSETDLEPPLLKKKK-TKVKFDDGAVFLAACSSGDTEEVLRMLDRGADINYA 69

  Fly    76 NVDGLTALHQACIDDNLDMVEFLVERGADINRQDNEGWTPLHATASCGFVSIARYLVENGADVAA 140
            ||||||||||||||||:|||.||||.||.||:.|||||.||||.||||::.||.||:..||.|..
Zfish    70 NVDGLTALHQACIDDNVDMVTFLVEHGACINQPDNEGWIPLHAAASCGYLDIAEYLISQGASVGV 134

  Fly   141 VNSDGDLALDLAIDVQHMAMIDYMEKMVQELNINVDEARKAEELAMLNDAKKWLRSDAAEVDRPH 205
            |||:|:..||:|   :..||.:.::..:....::::.|||.||..||.||::||.|.... |..|
Zfish   135 VNSEGETPLDIA---EEEAMEELLQNEINRQGVDIEAARKEEERIMLRDARQWLNSGQIN-DVRH 195

  Fly   206 PKTGATALHVAAAKGYTKVLGLLLAGRGNVDRQDNDGWTPLHAASHWGQRETAEMLVESLADMDI 270
            .|:|.|||||||||||.:||.||:....:|:.:|.|||||||||:|||:.|...:|||.|.|||:
Zfish   196 TKSGGTALHVAAAKGYAEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVEHLCDMDV 260

  Fly   271 RNYAGQSCIDVADRKIVKFLEELRANKRN---------KRRPSSQ-------------IRISDAM 313
            .|..||:..||||..|:.:||||: .|:|         |:.|..:             ::..:.:
Zfish   261 VNKVGQTAFDVADEDILGYLEELQ-KKQNLLLSEKKDVKKSPLIETTTTGDNNQSVKPLKSKETL 324

  Fly   314 ENHVEKTPTKLVRVEVR-------------------------TDATKDAENVK------------ 341
            ....|||..::..:|..                         :::..:|:..|
Zfish   325 LLEPEKTAPRIETLEPEKVDEEEGEGKKDESSCSSEEEEEEDSESENEADKTKSSVPSSVSNSTP 389

  Fly   342 --------------PNQQIHAVEHPVEEE-----------APWRRKLPRT-----PS----DSPT 372
                          |:.|:.....|.::.           |.||:.|.:|     |.    .:|.
Zfish   390 TTAPSSITVTSPTTPSNQVTTPTSPTKKSDNFTGGEPGCPALWRQGLRKTGISLVPKKAMVSTPA 454

  Fly   373 NNQVPDRELGTNSSETANDVILRRTQSFE-----NDQKFYQKYNELRARIKANSCPILPANANAN 432
            ....|..|...:.|..:..:.||:|.|:.     :..|..||..:....:::.|.|.|.::.:..
Zfish   455 GKLSPKEEDRKDESPASWRLGLRKTGSYGALAEISTTKEAQKEKDTAGVMRSASSPRLSSSLDNK 519

  Fly   433 AAAANKSNN----------NNNLSSNNYHNNNNNNNKSDLLLGYAAGTTT-------TTSTPTTT 480
            .....|...          ..::|:::.....|.::.:.|:   .:|:.|       ..::..|.
Zfish   520 DKEKEKEKTRLAYVAPTIPRRHVSTSDIDEKENRDSAASLV---RSGSYTRRRWEEDLKNSDGTA 581

  Fly   481 TTTTSSTFNNTHSNT---NNTSTTQQQPAAAAAASAAN-------------QLYSVQRSASLKDN 529
            :|..:|::..:.|:|   ..||:::..||.:::||:..             |.||:.||.|
Zfish   582 STNRTSSYQRSTSHTLALGRTSSSRDLPAKSSSASSLEPNNSKAWQPSSYYQSYSIHRSGS---- 642

  Fly   530 SMYYRKPTVTIATSTAATAINSPSTTVQTPP-IRSQVTAKIAEKSNNGSSSTASVSDAESSKPPP 593
              :.|:....:::::::|...:.:::|.:|. .||.::...||:|            ||..|...
Zfish   643 --FGRRHEDPLSSTSSSTTTTTTTSSVTSPTGHRSLLSRYWAEES------------AEKEKEKE 693

  Fly   594 KQSASNMI----------------------KNFFKSFVPPVRDEESETQRKAHAKRVRETRRSTQ 636
            |:||:.:.                      :...:|::.||||||||:||||.:::.|::|||||
Zfish   694 KESATVIPTINTAGTTTTTSTTGTVLGSDGRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQ 758

  Fly   637 GVTLDEIKSAEELVKKKNMGMANNNNNNNISTTTTNTISNSGNETSSASSTSAPTPSILGSNEQQ 701
            ||||.:::.||:.:.:..
Zfish   759 GVTLTDLQEAEKTIGRSR----------------------------------------------- 776

  Fly   702 DELQPPPPPTTPPPAVIPTTTTATDETEIEEVVSSPPAGQSQNDTTASFTLSAPVRRSLSTEGEA 766
                    ||.|          ..||.|.:|
Zfish   777 --------PTRP----------REDEKEEKE---------------------------------- 789

  Fly   767 DANSDPEGDQDQTVVSASYTIMPRRERLPESSENVESIRSPQKTEPVAKSSSEDKSEEPPHARPT 831
                    .||                                         ::|.||
Zfish   790 --------KQD-----------------------------------------KEKQEE------- 798

  Fly   832 DLPLIPAPAAPSTEAIKSSSAATTPAALESPVRLRDKRGLSGSQESETKSDTASPVSSHPDFNAR 896
                                                      .:|:|||.|         |:.:|
Zfish   799 ------------------------------------------KKETETKED---------DYRSR 812

  Fly   897 -DSLLSLYARRTTDSSAGGGGVGGGAGVGAGGASGAATSSSTSAAERRPSWRLKFDAGSKFKLED 960
             .|....|  ||                        :.:|||:|
Zfish   813 YRSFEEKY--RT------------------------SLASSTTA--------------------- 830

  Fly   961 ITSGGTYPPNNSTIIPSAPAVMAAANLSTTTGVQRRISSGPIALNASNQSLNSVGRPVSAPSEGT 1025
                       |:.|||:.:..:::..||                      :|:.||.|.
Zfish   831 -----------SSTIPSSSSSSSSSLYST----------------------SSLNRPNSL----- 857

  Fly  1026 NNSAYVTPSARKFETNATSTGATTAATTTSNSTSNSVSATSANHTTATAPNATSNHDDKDNDKEN 1090
                               ||.|   :|.|.||.::              :..|:..:||.|::.
Zfish   858 -------------------TGLT---STYSRSTRDT--------------DRESDRKEKDEDRDG 886

  Fly  1091 DNRTQ-TVIQRRRKP--KRRSTGVVH--IDMDELDPERQNESSNNDNEEKEKESGSERTSRSRLG 1150
            |:::| ..|:.||:|  |||||||..  .|.||.:|:..::|..:...|.:    |:|.|.:...
Zfish   887 DDKSQPRSIRDRRRPREKRRSTGVSFWTQDSDENEPDHPSDSEGSTKGEPQ----SDRLSSNDPL 947

  Fly  1151 STASTATTSESKSSSSN---------DKTENGDGIDYKALWEAEKLENDKLRQMLKQKDDEAVQT 1206
            || ||::|...:|.||.         .:.|..|..|||.|:|....||:||:..|:..:.|....
Zfish   948 ST-STSSTDRYESLSSRGIGESRRPYSRFEKDDSTDYKKLYEQILAENEKLKAQLRDTELELSDL 1011

  Fly  1207 RATL-------ERFANATTKNSLSELEKRERRAMERKLSELEEEL------KQLDAYKSDNHRLK 1258
            :..|       ||||:    .|..|:|||||||:|||:||:||||      ||:.:.:..|.||:
Zfish  1012 KLQLEKATQRQERFAD----RSQLEMEKRERRALERKISEMEEELKNLPQVKQVQSLRQYNERLQ 1072

  Fly  1259 EENAALIRVISKLSK 1273
            .||.||.||:||||:
Zfish  1073 AENRALARVLSKLSQ 1087

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mbs	NP_001246786.1	ANK	50..164	CDD:238125	74/113 (65%)
		Ank_2	53..142	CDD:289560	62/88 (70%)
		ANK repeat	78..109	CDD:293786	25/30 (83%)
		ANK	106..290	CDD:238125	91/183 (50%)
		ANK repeat	111..142	CDD:293786	18/30 (60%)
		ANK repeat	209..239	CDD:293786	17/29 (59%)
		Ank_2	213..300	CDD:289560	49/95 (52%)
		ANK repeat	241..272	CDD:293786	20/30 (67%)
		PRKG1_interact	1177..1272	CDD:292521	47/107 (44%)
ppp1r12a	XP_021330670.1	Ank_2	<47..312	CDD:330894	140/269 (52%)
		ANK	67..252	CDD:238125	104/188 (55%)
		ANK repeat	72..103	CDD:293786	25/30 (83%)
		ANK repeat	105..136	CDD:293786	18/30 (60%)
		ANK repeat	138..229	CDD:293786	38/94 (40%)
		ANK repeat	231..262	CDD:293786	20/30 (67%)
		ANK repeat	264..295	CDD:293786	13/31 (42%)
		PRKG1_interact	982..1086	CDD:318172	47/107 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	93	1.000	Domainoid score	I7498
eggNOG	1	0.900	-	-		E2759_KOG0505
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	352	1.000	Inparanoid score	I2230
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D225308at33208
OrthoFinder	1	1.000	-	-		FOG0002673
OrthoInspector	1	1.000	-	-		otm25006
orthoMCL	1	0.900	-	-		OOG6_105882
Panther	1	1.100	-	-	LDO	PTHR24179
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4930
SonicParanoid	1	1.000	-	-		X2914
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.860