DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Src64B and jak2b

DIOPT Version :9

Sequence 1:	NP_001286937.1	Gene:	Src64B / 48973	FlyBaseID:	FBgn0262733	Length:	553	Species:	Drosophila melanogaster
Sequence 2:	NP_571162.1	Gene:	jak2b / 30298	ZFINID:	ZDB-GENE-980526-123	Length:	1126	Species:	Danio rerio

Alignment Length:	648	Identity:	165/648 - (25%)
Similarity:	258/648 - (39%)	Gaps:	189/648 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 KRQDQELALAYPTG-------GYKKSDYTFGQTHINSSGGGNMGGVLGQKHNN-------GGSLD 58
            |::|.||..:...|       |.:|....:|:||....    :..||.:.|.|       ..|:.
Zfish   524 KKEDLELGESQGQGTFTKIFKGIRKEQGDYGETHKTEV----IVKVLDKAHRNYSESFFEAASMM 584

  Fly    59 SRYTPDPNHRGPLKIGGKGGVDIIRPRTTPTGVPGVVLKRVVV--ALYDYKSRDESDLSFMKGDR 121
            |:.|    |:..:...|          ....|...::::..|.  :|..|..:::|.:|.....:
Zfish   585 SQLT----HKHLVLTYG----------ICVCGDENIMVQEYVKFGSLDTYLKKNKSSVSVNILWK 635

  Fly   122 MEVIDDTESDWWRVVNLTTRQEGLIPLNFVAEERSVNSEDWFFENVLR-KEADKLL-----LAEE 180
            :||.....   |.::.|              ||:|:...:...:|:|. :|.|:.|     :...
Zfish   636 LEVAKQLA---WAMLYL--------------EEKSLAHGNVCAKNILLIREEDRALGNTPFIKLS 683

  Fly   181 NPRGTFLVRPSE--------------HNPNGYSLSVKDWEDGRGYHVKHYRIKPLDNGGYYIATN 231
            :|..:..|.|.|              .:|...||:...|..|.       .:..:.:||.....|
Zfish   684 DPGISITVLPREILVERIPWVPPECILDPKNLSLATDKWSFGT-------TLWEICSGGEQPLAN 741

  Fly   232 QTFPSLQALVMAYSKENALGLCHILSRPCPKPQPQMW-----------DLGPELRDK-------- 277
                      |..||::.....| ...|.||     |           |..|..|..
Zfish   742 ----------MDNSKKHLFYENH-HQLPAPK-----WTELANLINSCMDYEPTFRPSFKAIIRDL 790

  Fly   278 -------YEIPR-SEIQ----------------------------LLRKLGRGNFGEVFYGKWRN 306
                   |||.: |:|.                            .|::||:||||.|...::..
Zfish   791 NSLFCPDYEIVKESDIMPSRAAASIFNTGTFKNNEPVQFEERHLIFLQQLGKGNFGSVEMCRYDP 855

  Fly   307 SID-----VAVKTLREGTMS-TAAFLQEAAIMKKFRHNRLVALYAVC--SQEEPIYIVQEYMSKG 363
            ..|     ||||.|:..|.. ...|.:|..|:|..:|..:|....||  :....:.:|.||:..|
Zfish   856 LQDNTGEVVAVKKLQHSTTEHIRDFEREIEILKSLQHENIVKYKGVCYGAGRRNLRLVMEYLPYG 920

  Fly   364 SLLDFLREGDGRYLHFEDLIYIATQVASGMEYLESKQLIHRDLAARNVLIGENNVAKICDFGLAR 428
            ||.|:|.:...|..| :.|::.|:|:..|||||.:|:.||||||.||:|:......||.||||.:
Zfish   921 SLRDYLNKNRDRIDH-QKLVHYASQICKGMEYLATKRYIHRDLATRNILVESECRVKIGDFGLTK 984

  Fly   429 VIADDE--YCPKQGSRFPVKWTAPEAIIYGKFSIKSDVWSYGILLMELFTY-------------- 477
            |:..|:  |..|:....|:.|.|||::...|||:.|||||:|::|.|||||
Zfish   985 VLPQDKEYYKVKEPGESPIFWHAPESLTESKFSVASDVWSFGVVLYELFTYSDKLCSPPTVFLSM 1049

  Fly   478 ------GQ-VPYPGMHSREVIENIERGFRMPKPTNHYFPDNIYQLLLQCWDAVPEKRPTFEFL 533
                  || :.|      .:||.::||.|:|:|..  .|..:::::.:|||..|..||.|:.|
Zfish  1050 VGGDKQGQTIVY------HLIELLKRGNRLPQPMG--CPTEMFEIMQECWDNDPSLRPNFKEL 1104

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Src64B	NP_001286937.1	SH3_Src_like	99..150	CDD:212779	9/52 (17%)
		SH2_Src_family	158..259	CDD:199827	21/120 (18%)
		STYKc	285..537	CDD:214568	101/308 (33%)
		PTKc_Src_like	289..538	CDD:270630	99/276 (36%)
jak2b	NP_571162.1	B41	29..262	CDD:214604
		FERM_B-lobe	144..253	CDD:271216
		FERM_C_JAK2	258..370	CDD:270141
		SH2_Jak2	370..466	CDD:198242
		PKc_like	528..791	CDD:304357	58/320 (18%)
		Pkinase_Tyr	528..790	CDD:285015	58/319 (18%)
		PKc_like	829..1112	CDD:304357	100/285 (35%)
		TyrKc	834..1104	CDD:197581	99/278 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910