Protein Alignment Hsp70Bc and hspa4b

DIOPT Version :10

• Sequence 1: NP_650209.1
• Gene: Hsp70Bc / 48583
• FlyBaseID: FBgn0013279
• Length: 641
• Species: Drosophila melanogaster
• Sequence 2: NP_956151.1
• Gene: hspa4b / 334086
• ZFINID: ZDB-GENE-030131-6018
• Length: 840
• Species: Danio rerio
• Alignment Length: 709
• Identity: 200/709 - (28%)
• Similarity: 339/709 - (47%)
• Gaps: 116/709 - (16%)

  Fly     1 MPAIGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVF 65
            |..:|.|:|.....|.|.:.|.:|.:||:..:|.||:.|:|....|.||..||:|:..|.:|||.
Zfish     1 MSVVGFDVGFLNCYVAVARAGGIETVANEYSDRCTPACVSFGPRNRSIGAAAKSQMVTNCKNTVH 65

  Fly    66 DAKRLIGRKYDD-------PKIAEDMKHWPFKVVSDGGKPKIGVEYKGESKRFAPEEISSMVLTK 123
            ..||..||.:.|       |.:..|:...|      .|...:.|.|..|.|.|:.|::::|:|||
Zfish    66 GFKRFHGRAFSDPFVQNLKPSLVYDLAQMP------SGTTGLKVMYMEEEKVFSIEQVTAMLLTK 124

  Fly   124 MKETAEAYLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKN--- 185
            :|||||:.|.:.:.|.||:||.::.|::|::..||..|||||.||::||.||.|||||:.|.
Zfish   125 LKETAESALKKPVADCVISVPCFYTDAERRSVIDAAQIAGLNCLRLMNETTAVALAYGIYKQDLP 189

  Fly   186 --LKGERNVLIFDLGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLAEEFKRKYK 248
              .:..|||:..|:|...:.||:...::|.| ::.:||.|..:||:.||.|||.:..|||..|||
Zfish   190 APEEKPRNVVFVDIGHSGYQVSVCAFNKGKL-KILATAFDPEMGGKYFDERLVKYFCEEFVVKYK 253

  Fly   249 KDLRSNPRALRRLRTAAERAKRTLS-SSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTL 312
            .|.::.||||.||....|:.|:.:| :|::..:.|:......|..::::||:|||:|||:.....
Zfish   254 LDAKTKPRALIRLFQECEKLKKLMSANSSDLPLNIECFMNDVDVSSRLNRAQFEEMCADILARVE 318

  Fly   313 QPVEKALNDAKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAI 377
            .|:...|..|.:.|..||.:.:|||::|:|.::..:.:|| ||..:.::|.|||||.|.|:|.||
Zfish   319 PPLRSLLEQAHLKKDDIHAVEIVGGASRMPAIKERISKFF-GKEPSTTLNADEAVARGCALQCAI 382

  Fly   378 LSGDQSGKIQDVLLVDVAPLSLGIE----TAGGVMTKLIERNCRI-PCKQTKTFS---TYSDNQP 434
            ||  .:.|:::..:.:|.|..:.::    ...||      .:|.: |......||   |:...:|
Zfish   383 LS--PAFKVREFSITEVVPFPISLKWNSAAEDGV------SDCEVFPKNHAAPFSKVLTFYRREP 439

  Fly   435 GVSIQVYEGERAMTKDNNALGTFDLSGIPPAPRG-VPQIEVTFDLDANGILNVSA---------- 488
            ......|...:.:...:..:|.:.:..:.|...| ..:::|...::.:||.:||:
Zfish   440 FTLDAYYNSPKELPYPDPTIGQYVVQKVVPQASGESSKVKVKVRVNIHGIFSVSSASLVEVQKSE 504

  Fly   489 -------------------------------------------KEMST----GK----------A 496
                                                       :||.|    ||          |
Zfish   505 EEEESMETEQSTEKENENKMQVDQEEQKTPETEQENGEKKPGTEEMETSAEEGKQEKKSDQPPQA 569

  Fly   497 KNITIK----------NDKGRLSQAEIDRMVNEAEKYADEDEKHRQRITSRNALESYVFNVKQSV 551
            |...:|          |.:.:|:...::..|....|...:|:..::|..::|.:|.||:.::..:
Zfish   570 KKPKVKTKVLDLPIENNPQWQLANDMLNLFVESEGKMIMQDKLEKERNDAKNYVEEYVYEMRDKL 634

  Fly   552 EQAPAGKLDEADKNSVLDKCNETIRWL-DSNTTAEKEEFDHKMEELTRHCSPIMTKMHQ 609
            .......:.|:|::.:..|..:|..|| :......|:.:..|:.||.....||..:..:
Zfish   635 HGIFEKFVTESDRDVLSLKLEDTEVWLYEDGEDQPKQIYIDKLAELKNLGQPIQDRYRE 693

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hsp70Bc	NP_650209.1	PTZ00009	2..641	CDD:240227	199/708 (28%)
hspa4b	NP_956151.1	ASKHA_NBD_HSP70_HSPA4	2..382	CDD:466843	145/387 (37%)
		SCP-1	<521..802	CDD:114219	30/173 (17%)