Protein Alignment Hsp70Bb and Hspa9

DIOPT Version :10

• Sequence 1: NP_524927.2
• Gene: Hsp70Bb / 48582
• FlyBaseID: FBgn0013278
• Length: 641
• Species: Drosophila melanogaster
• Sequence 2: NP_001094128.2
• Gene: Hspa9 / 291671
• RGDID: 1311806
• Length: 679
• Species: Rattus norvegicus
• Alignment Length: 635
• Identity: 316/635 - (49%)
• Similarity: 424/635 - (66%)
• Gaps: 33/635 - (5%)

  Fly     4 IGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFT-DSERLIGDPAKNQVAMNPRNTVFDA 67
            :|||||||.|||.|.:..:.:::.|.:|.|||||.|||| |.|||:|.|||.|...||.||.:..
  Rat    56 VGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTPDGERLVGMPAKRQAVTNPNNTFYAT 120

  Fly    68 KRLIGRKYDDPKIAEDMKHWPFKVV--SDGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEA 130
            ||||||:||||::.:|.|:.|||:|  |:|   ...||..|  |.::|.:|.:.||.|||||||.
  Rat   121 KRLIGRRYDDPEVQKDTKNVPFKIVRASNG---DAWVEAHG--KLYSPSQIGAFVLMKMKETAEN 180

  Fly   131 YLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIF 195
            |||.:..:|||||||||||||||||||||.|:||||||:|||||||||||||||:  .::.:.::
  Rat   181 YLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKS--EDKVIAVY 243

  Fly   196 DLGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLAEEFKRKYKKDLRSNPRALRR 260
            ||||||||:|||.|.:| :|||:||.|||.|||||||..|:.|:.:||||:...||..:..||:|
  Rat   244 DLGGGTFDISILEIQKG-VFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQR 307

  Fly   261 LRTAAERAKRTLSSSTEATIEIDALFEG----QDFYTKVSRARFEELCADLFRNTLQPVEKALND 321
            :|.|||:||..||||.:..|.:..|...    :....|::||:||.:..||.:.|:.|.:||:.|
  Rat   308 VREAAEKAKCELSSSVQTDINLPYLTMDASGPKHLNMKLTRAQFEGIVTDLIKRTIAPCQKAMQD 372

  Fly   322 AKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKI 386
            |::.|..|.:::||||.||:||||..:|:.| |:..:.::|||||||.|||:|..:|:||    :
  Rat   373 AEVSKSDIGEVILVGGMTRMPKVQQTVQDLF-GRAPSKAVNPDEAVAIGAAIQGGVLAGD----V 432

  Fly   387 QDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYSDNQPGVSIQVYEGERAMTKDN 451
            .||||:||.||||||||.|||.||||.||..||.|:::.|||.:|.|..|.|:|.:|||.|..||
  Rat   433 TDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDN 497

  Fly   452 NALGTFDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRM 516
            ..||.|.|.|||||||||||||||||:|||||::||||:..||:.:.|.|:: .|.||:.:|:.|
  Rat   498 KLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQS-SGGLSKDDIENM 561

  Fly   517 VNEAEKYADEDEKHRQRITSRNALESYVFNVKQSVE----QAPAGKLDEADK-NSVLDKCNETIR 576
            |..|||||:||.:.::|:.:.|..|..:.:.:..:|    |.||   ||.:| ...:.|..|.:.
  Rat   562 VKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPA---DECNKLKEEISKMRELLA 623

  Fly   577 WLDSNTTAEKEEFDHKMEELTRHCSPIMTKMHQQGAGAAGGPG-ANCGQQ 625
            ..||.|   .|........|.:....:....:::.|....|.| ::.|:|
  Rat   624 RKDSET---GENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSSTGEQ 670

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hsp70Bb	NP_524927.2	PTZ00009	2..641	CDD:240227	316/635 (50%)
Hspa9	NP_001094128.2	Interaction with NFS1. /evidence=ECO:0000250|UniProtKB:P38646	1..432		207/388 (53%)
		dnaK	52..656	CDD:234715	311/619 (50%)
		Nucleotide-binding domain (NBD). /evidence=ECO:0000250|UniProtKB:P38646	63..431		199/376 (53%)
		Interaction with FXN and ISCU. /evidence=ECO:0000250|UniProtKB:P38646	432..679		109/246 (44%)
		Interdomain linker. /evidence=ECO:0000250|UniProtKB:P38646	432..441		5/8 (63%)
		Substrate-binding domain (SBD). /evidence=ECO:0000250|UniProtKB:P38646	442..679		103/236 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	655..679		5/16 (31%)