Protein Alignment Hsp70Bb and Hspa8

DIOPT Version :10

• Sequence 1: NP_524927.2
• Gene: Hsp70Bb / 48582
• FlyBaseID: FBgn0013278
• Length: 641
• Species: Drosophila melanogaster
• Sequence 2: NP_112442.2
• Gene: Hspa8 / 15481
• MGIID: 105384
• Length: 646
• Species: Mus musculus
• Alignment Length: 643
• Identity: 483/643 - (75%)
• Similarity: 554/643 - (86%)
• Gaps: 4/643 - (0%)

  Fly     2 PAIGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFD 66
            ||:|||||||||||||:|||||||||||||||||||||||||:||||||.|||||||||.|||||
Mouse     5 PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFD 69

  Fly    67 AKRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAY 131
            |||||||::||..:..|||||||.||:|.|:||:.||||||:|.|.|||:||||||||||.||||
Mouse    70 AKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAY 134

  Fly   132 LGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196
            ||:::|:||:||||||||||||||||||.|||||||||||||||||:||||||.:..||||||||
Mouse   135 LGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFD 199

  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLAEEFKRKYKKDLRSNPRALRRL 261
            |||||||||||||::| :|||:||||||||||||||||:|.|...|||||:|||:..|.||:|||
Mouse   200 LGGGTFDVSILTIEDG-IFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRL 263

  Fly   262 RTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDK 326
            |||.|||||||||||:|:||||:|:||.||||.::|||||||.|||||.||.||||||.|||:||
Mouse   264 RTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDK 328

  Fly   327 GQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLL 391
            .||||||||||||||||:|.|||:||:||.||.||||||||||||||||||||||:|..:||:||
Mouse   329 SQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLL 393

  Fly   392 VDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYSDNQPGVSIQVYEGERAMTKDNNALGT 456
            :||.||||||||||||||.||:||..||.|||:||:|||||||||.|||||||||||||||.||.
Mouse   394 LDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGK 458

  Fly   457 FDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAE 521
            |:|:|||||||||||||||||:||||||||||.:.||||...|||.|||||||:.:|:|||.|||
Mouse   459 FELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAE 523

  Fly   522 KYADEDEKHRQRITSRNALESYVFNVKQSVE-QAPAGKLDEADKNSVLDKCNETIRWLDSNTTAE 585
            ||..||||.|.:::|:|:||||.||:|.:|| :...||:::.||..:||||||.|.|||.|.|||
Mouse   524 KYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAE 588

  Fly   586 KEEFDHKMEELTRHCSPIMTKMHQQGAGAAGG-PGANCGQQAGGFGG-YSGPTVEEVD 641
            ||||:|:.:||.:.|:||:||::|...|..|| ||...|..|...|| .||||:||||
Mouse   589 KEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD 646

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hsp70Bb	NP_524927.2	PTZ00009	2..641	CDD:240227	481/641 (75%)
Hspa8	NP_112442.2	PTZ00009	1..613	CDD:240227	465/608 (76%)
		Nucleotide-binding domain (NBD). /evidence=ECO:0000250|UniProtKB:P11142	2..386		309/381 (81%)
		Interaction with BAG1. /evidence=ECO:0000250	186..377		150/191 (79%)
		Substrate-binding domain (SBD). /evidence=ECO:0000250|UniProtKB:P11142	394..509		92/114 (81%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	614..646		15/31 (48%)