DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NOS2 and Nos

DIOPT Version :9

Sequence 1:	NP_000616.3	Gene:	NOS2 / 4843	HGNCID:	7873	Length:	1153	Species:	Homo sapiens
Sequence 2:	NP_523541.2	Gene:	Nos / 34495	FlyBaseID:	FBgn0011676	Length:	1349	Species:	Drosophila melanogaster

Alignment Length:	1178	Identity:	520/1178 - (44%)
Similarity:	695/1178 - (58%)	Gaps:	90/1178 - (7%)

- Green bases have known domain annotations that are detailed below.

Human    31 APCATSSPVTQDDLQYHNL--SKQQNESPQPLVETGKKSPESLVKLD--ATPLSSPRHVRIKNWG 91
            |.|..|...|........|  |.:..:.|:.:.:..:....|.:.||  ...|...:.:|:||..
  Fly   159 AGCPPSGSCTASGKSSRELSPSPKNQQQPRKMSQDYRSRAGSFMHLDDEGRSLLMRKPMRLKNIE 223

Human    92 SGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKE 156
            ......||||.|.:.||:|...:|..|||...:.....|..    |.:|..|.:|:.||:.|.|.
  Fly   224 GRPEVYDTLHCKGREILSCSKATCTSSIMNIGNAAVEARKS----DLILEHAKDFLEQYFTSIKR 284

Human   157 AKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAR 221
            .....|..|.:.|.:.|||||.||||..|||:..|.||||:.|||||||||.|||||.|..:|..
  Fly   285 TSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTS 349

Human   222 EMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEF 286
            .|||.||.|::|:||.||:|||||:||||:|.|||:|:||.|||.||||:..||.|.|||.||||
  Fly   350 GMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKIIGDPMNVEF 414

Human   287 TQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPA 351
            |::|..||||.|...:|::|||:.|||.||:.|:.||:|:|||.:.|||:|||.:|.|:||||||
  Fly   415 TEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDLGLRWYALPA 479

Human   352 VANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVV 416
            |::||.:|||::|....|:||||.||||.|:.||..|.|:||.|..:|.|:|....|||||:|||
  Fly   480 VSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPTSLWKDKAVV 544

Human   417 EINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEML 481
            |:|||||||:|.:||||:|||:|:|||||:.:||.:.|.||||||||:|||:||||||||||||.
  Fly   545 EMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSITPVFHQEMA 609

Human   482 NYVLSPFYYYQVEAWKTHVWQ----DEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTIL 542
            .|.|.|.:.||..||:||||:    :.|.:..||:...|.:.:||.|...|..:.::.|::.|:|
  Fly   610 LYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAVKFTSKLFGRALSKRIKATVL 674

Human   543 FATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKK 607
            :||||||||..|..|..|...|||.::.||..|.:|.:|.|.||:||.|||||||.|.|||...:
  Fly   675 YATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPENGELFSQ 739

Human   608 SLFMLK----------------------------------------------------------- 613
            .|:.::
  Fly   740 ELYAMRVQESSEHGLQDSSIGSSKSFMKASSRQEFMKLPLQQVKRIDRWDSLRGSTSDTFTEETF 804

Human   614 -ELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTF 677
             .|:| .|:|||.||||.||.||||...:|..|..||..:|..:..|||:.|||.:||.||.:.|
  Fly   805 GPLSN-VRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCGQEQSFRKWAPEVF 868

Human   678 KAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSR-Q 741
            |.|||||.:..::.:....|...|.:...:..|||..:....|..:||..|.|.|...:.|:: .
  Fly   869 KLACETFCLDPEESLSDASLALQNDSLTVNTVRLVPSANKGSLDSSLSKYHNKKVHCCKAKAKPH 933

Human   742 NL-QSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEA 805
            || :....::.|:|:|: |  ..||.|.||:|:|:.|.|:..||.|:|.|:|....|.:.::|:.
  Fly   934 NLTRLSEGAKTTMLLEI-C--APGLEYEPGDHVGIFPANRTELVDGLLNRLVGVDNPDEVLQLQL 995

Human   806 LDE----SGSY--WVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQ 864
            |.|    :|.:  |....::||.:|...|..|.|:||||::.||..||....:..:::|||.|..
  Fly   996 LKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADKERLELLVN 1060

Human   865 PSE-YSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVV 928
            .|. |..|:....|..|:|||||||.|..|..||:||..|:|||||||||......|||||||:|
  Fly  1061 DSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDEIHLTVAIV 1125

Human   929 TYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFW 993
            .|...||||...:||||.:|:.|:..|.:..|||:|.|||||.|.|.|.||||||||||||||||
  Fly  1126 KYRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGFHLPSDRSRPIILIGPGTGIAPFRSFW 1190

Human   994 Q--QRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYV 1056
            |  |.|.|.. ...:..:|.|.||||..|.| :|.||..|:.:..:|..|..|.||....||.||
  Fly  1191 QEFQVLSDLD-PTAKLPKMWLFFGCRNRDVD-LYAEEKAELQKDQILDRVFLALSREQAIPKTYV 1253

Human  1057 QDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQK 1121
            ||::.|:..| :.:::.:|.||:||||||.||..|..|:::.:|.|.:.:|.:||.:...|:.:.
  Fly  1254 QDLIEQEFDS-LYQLIVQERGHIYVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETFLLTLRDES 1317

Human  1122 RYHEDIFG 1129
            ||||||||
  Fly  1318 RYHEDIFG 1325

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NOS2	NP_000616.3	DINNN-motif, mediates interaction with SPSB1, SPSB2 and SPSB4. /evidence=ECO:0000269\|PubMed:21199876	23..27
		NOS_oxygenase_euk	84..496	CDD:238410	229/411 (56%)
		Calmodulin-binding. /evidence=ECO:0000255	509..529		6/19 (32%)
		CysJ	538..1127	CDD:223446	261/659 (40%)
		Flavodoxin_1	541..672	CDD:278677	66/190 (35%)
		FNR_like	736..1132	CDD:297884	178/405 (44%)
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	229/411 (56%)
		CysJ	669..1323	CDD:223446	261/660 (40%)
		Flavodoxin_1	673..863	CDD:278677	66/190 (35%)
		Nitric_oxide_synthase	931..1328	CDD:99799	177/401 (44%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	525	1.000	Domainoid score	I292
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1114	1.000	Inparanoid score	I227
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46820
OrthoDB	1	1.010	-	-		D56017at33208
OrthoFinder	1	1.000	-	-		FOG0001418
OrthoInspector	1	1.000	-	-		otm42050
orthoMCL	1	0.900	-	-		OOG6_103176
Panther	1	1.100	-	-	O	PTHR19384
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	10	9.980