DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NOS1 and Nos

DIOPT Version :9

Sequence 1:	NP_001191147.1	Gene:	NOS1 / 4842	HGNCID:	7872	Length:	1468	Species:	Homo sapiens
Sequence 2:	NP_523541.2	Gene:	Nos / 34495	FlyBaseID:	FBgn0011676	Length:	1349	Species:	Drosophila melanogaster

Alignment Length:	1403	Identity:	620/1403 - (44%)
Similarity:	832/1403 - (59%)	Gaps:	169/1403 - (12%)

- Green bases have known domain annotations that are detailed below.

Human   105 THLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYD----- 164
            |....|..|:|.                  ||..|..|           |..:.|.|..|
  Fly    58 TQATPTLNGNGL------------------LSGNPNGG-----------GGDSSPSHEVDHPGGA 93

Human   165 -DGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGV----K 224
             ..|.||.||.::|...|...:.....|:....:.||.|..::.|::...|...||..||    .
  Fly    94 QGAQAAGGLPSSSGTPLRHHKRASISTASPPIRERRGTNTSIVVELDGSGSGSGSGGGGVGVGQG 158

Human   225 GGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSG 289
            .|.|.....         ...|||.:.|....:|                       ::||....
  Fly   159 AGCPPSGSC---------TASGKSSRELSPSPKN-----------------------QQQPRKMS 191

Human   290 KQSPTKNGS---------------PSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGS 339
            :...::.||               |      :::||.|....:.||||.|......|::..|..|
  Fly   192 QDYRSRAGSFMHLDDEGRSLLMRKP------MRLKNIEGRPEVYDTLHCKGREILSCSKATCTSS 250

Human   340 IMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDT 404
            ||:....|   .:.|....:...||:|::||::||||..|.||..|.::|.:.|:||..|||.:|
  Fly   251 IMNIGNAA---VEARKSDLILEHAKDFLEQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTET 312

Human   405 ELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQ 469
            |||||||.||||:|||:||||||||||||.|..||..|||..||||:||||||||||||||||||
  Fly   313 ELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQ 377

Human   470 RTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGN 534
            |||.|||:|:||:|||.||||||.||..:|||.||:|||:|.:.|||.....:|:|||::.|||:
  Fly   378 RTDAKHDYRIWNNQLISYAGYKQADGKIIGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGH 442

Human   535 DPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIG 599
            ||:.|..||||:||||:.|||||||.||||:||.|||||:||.::||::|:|..||||||.||||
  Fly   443 DPDYFDYPPELILEVPLTHPKFEWFSDLGLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIG 507

Human   600 VRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFI 664
            .|:.||.:|.|:||.||.||.||.|..:|||||:|:||:|||||:|:||..|||||||:|:|||:
  Fly   508 SRNLCDTNRRNMLETVALKMQLDTRTPTSLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFM 572

Human   665 KHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWK---GTNG 726
            ||.|||.:.|.||||||:|||||:||||||||||||..|.|.||||||...|.|||||   |.:.
  Fly   573 KHFENESKLRNGCPADWIWIVPPLSGSITPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESK 637

Human   727 TPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVM 791
            ....||...||::|.||||::||.|:|::||:|||:|||||||||:.|||.|||:..|||:|::.
  Fly   638 GKKPRRKFNFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIY 702

Human   792 SMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKG 856
            .|.:|||..:|||.|::||.|||||||||||||.|...|..||    |||..:
  Fly   703 CMSDYDISSIEHEALLIVVASTFGNGDPPENGELFSQELYAMR----VQESSE------------ 751

Human   857 PPLPNGDTEVHGLAAARDSQHRSYKVRFNSVSSYS----------------DSQKSSGDGPDLRD 905
                      |||   :||...|.| .|...||..                ||.:.|     ..|
  Fly   752 ----------HGL---QDSSIGSSK-SFMKASSRQEFMKLPLQQVKRIDRWDSLRGS-----TSD 797

Human   906 NF--ESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRT 968
            .|  |:.|||:||||:||.|||.|||:|||||..||.:|.||||||:|::..|||:||||::||.
  Fly   798 TFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCGQEQSFRK 862

Human   969 WAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSA 1033
            ||.:|||.||:.||:..:.::..|:.:| .|| |...|..||...|....|...||..|.|:|..
  Fly   863 WAPEVFKLACETFCLDPEESLSDASLAL-QND-SLTVNTVRLVPSANKGSLDSSLSKYHNKKVHC 925

Human  1034 ARLLSR-QNL-QSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPV 1096
            .:..:: .|| :..:.:::|:.:.:...|   |:|:||||:|:||.|..:||:.|:.||......
  Fly   926 CKAKAKPHNLTRLSEGAKTTMLLEICAPG---LEYEPGDHVGIFPANRTELVDGLLNRLVGVDNP 987

Human  1097 NQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEK 1161
            :::::::||:|:.|:.|:...|....::||.|:......:.|:||||:...|...|.......:|
  Fly   988 DEVLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADK 1052

Human  1162 QRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDE 1226
            :||.:|......||:|:..:.|.:::|||||||.:.||.|||.||:.||||:|||||||....||
  Fly  1053 ERLELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDE 1117

Human  1227 VHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTG 1291
            :|||||||.||..||:|...:||||::|:.::||:.:..|||.|..||||.:...|.||:|||||
  Fly  1118 IHLTVAIVKYRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGFHLPSDRSRPIILIGPGTG 1182

Human  1292 IAPFRSFWQQRQF--DIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSRE 1354
            |||||||||:.|.  |:......| .|.|.||||...:| :|.||..:.:...:...::.|.|||
  Fly  1183 IAPFRSFWQEFQVLSDLDPTAKLP-KMWLFFGCRNRDVD-LYAEEKAELQKDQILDRVFLALSRE 1245

Human  1355 PDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVF 1419
            ...||.||||:::::. :|:|:.:.::.||||||||||||..|.:.|::.:..:.:.|..:...|
  Fly  1246 QAIPKTYVQDLIEQEF-DSLYQLIVQERGHIYVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETF 1309

Human  1420 ISRMRDDNRYHEDIFGVTLRTYEV------TNRLRSES 1451
            :..:||::||||||||:||||.|:      |.|:|..|
  Fly  1310 LLTLRDESRYHEDIFGITLRTAEIHTKSRATARIRMAS 1347

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NOS1	NP_001191147.1	PDZ_signaling	15..98	CDD:238492
		NOS_oxygenase_euk	305..716	CDD:238410	252/410 (61%)
		CysJ	759..1433	CDD:223446	292/695 (42%)
		Flavodoxin_1	762..969	CDD:278677	104/224 (46%)
		Nitric_oxide_synthase	1035..1438	CDD:99799	165/406 (41%)
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	253/416 (61%)
		CysJ	669..1323	CDD:223446	292/696 (42%)
		Flavodoxin_1	673..863	CDD:278677	104/224 (46%)
		Nitric_oxide_synthase	931..1328	CDD:99799	165/402 (41%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	525	1.000	Domainoid score	I292
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H37327
Inparanoid	1	1.050	1114	1.000	Inparanoid score	I227
Isobase	1	0.950	-	0.75	Normalized mean entropy	S1636
OMA	1	1.010	-	-		QHG46820
OrthoDB	1	1.010	-	-		D56017at33208
OrthoFinder	1	1.000	-	-		FOG0001418
OrthoInspector	1	1.000	-	-		otm42050
orthoMCL	1	0.900	-	-		OOG6_103176
Panther	1	1.100	-	-	O	PTHR19384
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3122
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	13	12.930