DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pkc53E and Prkci

DIOPT Version :9

Sequence 1:	NP_725626.1	Gene:	Pkc53E / 48311	FlyBaseID:	FBgn0003091	Length:	679	Species:	Drosophila melanogaster
Sequence 2:	NP_114448.1	Gene:	Prkci / 84006	RGDID:	620961	Length:	596	Species:	Rattus norvegicus

Alignment Length:	674	Identity:	222/674 - (32%)
Similarity:	311/674 - (46%)	Gaps:	207/674 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 PQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAW 75
            |::.|.  ...||:  ||..|:||.:.:.::....|.|.|:.|.:...|:.|.|.|        |
  Rat   110 PERPGM--PCPGED--KSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRI--------W 162

  Fly    76 MGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLL 140
             |.|:||::|..|..:|||:||:.||..|
  Rat   163 -GLGRQGYKCINCKLLVHKKCHKLVTIEC------------------------------------ 190

  Fly   141 YGIYHQGLKCSACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMD 205
                                                                     ||:.:|.:
  Rat   191 ---------------------------------------------------------GRHSLPPE 198

  Fly   206 PNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSR 270
            |           ::|  .|||.         ::|...:|:.
  Rat   199 P-----------MMP--MDQSS---------MHPDHTQTVI------------------------ 217

  Fly   271 NDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTN 335
                             |.|                |.:.:..|     |...:|:.|      |
  Rat   218 -----------------PYN----------------PSSHESLD-----QVGEEKEAM------N 238

  Fly   336 THTSSK-KDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEK 399
            |..|.| ...:...||:.::|:|:||:.||||...|.::.:||:|::||:::..|:|::....||
  Rat   239 TRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEK 303

  Fly   400 RVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFF 464
            .|.......||||.|||||||..|||||:||||||||||.:|:..|..|..|.||:|||:..|.:
  Rat   304 HVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNY 368

  Fly   465 LHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKS 529
            ||.:||:||||||||||||::||:|:.|:|||||.:....||.||||||:||||||:..:.||.|
  Rat   369 LHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFS 433

  Fly   530 VDWWAYGVLLYEMLVGQPPF---------DGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLT 585
            |||||.|||::||:.|:.||         |...|:.||..|.:..:..|:|||.:|....|.||.
  Rat   434 VDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLN 498

  Fly   586 KQPNKRLGC-GSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDL 649
            |.|.:|||| ..:|..|::.|||||.:||:.:|.::|.|||||.|.....:.|||.|||:|...|
  Rat   499 KDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQL 563

  Fly   650 TPTDKVFMMNLDQSEFVGFSYMNP 673
            ||.|...:..:|||||.||.|:||
  Rat   564 TPDDDDIVRKIDQSEFEGFEYINP 587

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkc53E	NP_725626.1	C1_cPKC_rpt1	44..110	CDD:410383	23/65 (35%)
		C1_cPKC_rpt2	120..173	CDD:410386	0/52 (0%)
		C2_PKC_alpha_gamma	177..307	CDD:175992	12/129 (9%)
		STKc_cPKC	353..672	CDD:270739	165/328 (50%)
Prkci	NP_114448.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		Regulatory domain. /evidence=ECO:0000250	2..253		53/338 (16%)
		Required for interaction with RAB2. /evidence=ECO:0000250	2..28
		PB1_aPKC	26..108	CDD:99725
		Interaction with PARD6A. /evidence=ECO:0000250	72..91
		Pseudosubstrate. /evidence=ECO:0000250	125..134		3/8 (38%)
		C1_aPKC	139..193	CDD:410344	24/155 (15%)
		STKc_aPKC_iota	233..596	CDD:270769	175/361 (48%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166343091
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D614710at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.850