DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pkc53E and prkcbb

DIOPT Version :9

Sequence 1:	NP_725626.1	Gene:	Pkc53E / 48311	FlyBaseID:	FBgn0003091	Length:	679	Species:	Drosophila melanogaster
Sequence 2:	NP_957272.1	Gene:	prkcbb / 393953	ZFINID:	ZDB-GENE-040426-1178	Length:	670	Species:	Danio rerio

Alignment Length:	683	Identity:	453/683 - (66%)
Similarity:	545/683 - (79%)	Gaps:	28/683 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDF 65
            |:|.::::|:.:.....|.|          |:|||::|||..||:|.|||||||||||||||.||
Zfish     1 MAEPANSDGEERSAPMRGFA----------RQGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDF 55

  Fly    66 ICGYQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGP 130
            |        | ||||||||||||.:||||||||:|||.|||.|||..||.|:::|.|:..||:.|
Zfish    56 I--------W-GFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSP 111

  Fly   131 TFCDHCGSLLYGIYHQGLKCSACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQI 195
            ||||||||||||:.|||::|..|.||:|.||..|||||||.|||||||||.:...:|.|:|||.|
Zfish   112 TFCDHCGSLLYGLIHQGMRCDHCMMNIHKRCVANVPSLCGTDHTERRGRIQITAEIKNNVLTVSI 176

  Fly   196 KEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILI 260
            ||.:||:|||||||||||||:|||||.|.:||:||:|||.||||.||||.|::||..|||||:.:
Zfish   177 KEAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCCLNPTWNETFTFNLKESDKDRRLSV 241

  Fly   261 EVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPC-ADDEQDLLKLKQKPSQ 324
            |:||||.||||||||:|||||||:.|...:||||||:|:||||:|||. .:.|:...:|:||..:
Zfish   242 EIWDWDLTSRNDFMGSLSFGISELQKQGVDGWFKLLSQEEGEYFNVPVPPEGEEGNEELRQKFER 306

  Fly   325 KKPMVMRSD-TNTHTSSK------KDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILK 382
            .|....::| ::::..||      :|.::.:||||:.||||||||||:||||||::||:||||||
Zfish   307 AKIGPSKTDGSSSNAISKFDSNGNRDRMKLSDFNFLMVLGKGSFGKVMLAERKGADELFAIKILK 371

  Fly   383 KDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFK 447
            |||:||||||||||:|||||||..|||||.||||||||||||:|||||:||||||:.|||.||||
Zfish   372 KDVVIQDDDVECTMVEKRVLALSGKPPFLTQLHSCFQTMDRLYFVMEYINGGDLMYHIQQVGKFK 436

  Fly   448 EPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGT 512
            ||.|||||||||.||||||:||::|||||||||:|||:||:|||||||||||::...||||||||
Zfish   437 EPHAVFYAAEIAIGLFFLHSKGVIYRDLKLDNVMLDAEGHIKIADFGMCKENMLDGVTTKTFCGT 501

  Fly   513 PDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAK 577
            |||||||||.||||||||||||:||||||||.||||||||||:|||.:|.:|:||||||:||||.
Zfish   502 PDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQPPFDGEDEDELFQSIMEHHVSYPKSMSKEAV 566

  Fly   578 EACKGFLTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQF 642
            ..|||.:||.|.||||||..||.|:|.|.|||.:||||:|:||||||||||...| |..|||:.|
Zfish   567 AICKGLMTKHPGKRLGCGPEGERDIREHGFFRYMDWEKLEHREVQPPFKPKACGR-DAENFDRFF 630

  Fly   643 TSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEY 675
            |.....|||.|:..:|||||.||.|||::|||:
Zfish   631 TRHPPVLTPPDQEVIMNLDQDEFEGFSFINPEF 663

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkc53E	NP_725626.1	C1_cPKC_rpt1	44..110	CDD:410383	48/65 (74%)
		C1_cPKC_rpt2	120..173	CDD:410386	34/52 (65%)
		C2_PKC_alpha_gamma	177..307	CDD:175992	89/129 (69%)
		STKc_cPKC	353..672	CDD:270739	241/318 (76%)
prkcbb	NP_957272.1	C1_1	36..88	CDD:278556	45/60 (75%)
		C1_1	101..151	CDD:278556	32/49 (65%)
		C2_PKC_alpha_gamma	158..288	CDD:175992	89/129 (69%)
		STKc_cPKC_beta	338..660	CDD:270767	245/322 (76%)
		S_TKc	339..597	CDD:214567	205/257 (80%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170583303
Domainoid	1	1.000	428	1.000	Domainoid score	I580
eggNOG	1	0.900	-	-		E1_KOG0696
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	955	1.000	Inparanoid score	I321
OMA	1	1.010	-	-		QHG51944
OrthoDB	1	1.010	-	-		D143719at33208
OrthoFinder	1	1.000	-	-		FOG0000117
OrthoInspector	1	1.000	-	-		otm24881
orthoMCL	1	0.900	-	-		OOG6_103255
Panther	1	1.100	-	-	LDO	PTHR24356
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	1	1.000	-	-		X125
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.800