DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pkc53E and pck1

DIOPT Version :9

Sequence 1:	NP_725626.1	Gene:	Pkc53E / 48311	FlyBaseID:	FBgn0003091	Length:	679	Species:	Drosophila melanogaster
Sequence 2:	NP_593737.2	Gene:	pck1 / 2542293	PomBaseID:	SPAC17G8.14c	Length:	988	Species:	Schizosaccharomyces pombe

Alignment Length:	671	Identity:	238/671 - (35%)
Similarity:	331/671 - (49%)	Gaps:	115/671 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    31 RKGALKKKN---VFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGFQCQVCSYVV 92
            |:||:::|.   |.:...|.|:.|.|.|...|:.|.:..     .|:      .|.||:.||:|.
pombe   396 RQGAIRQKKEDVVASQVGHQFVQRQFYQIMRCAVCAELF-----SYS------PGLQCENCSFVC 449

  Fly    93 HKRCHEYVTFICPGKDKGIDSD----SPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKCSAC 153
            ||:|...|...|..:.....||    ..:..|.||||...|..:|.|||..|........||..|
pombe   450 HKKCVTKVLASCIAQSNSEKSDFGGLRYRIPHRFEPFNSLGAQWCAHCGFFLPLRRKDCFKCVEC 514

  Fly   154 DMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKL 218
            .:..|.:|...:|..||..:           ::|..|||......|...|..||
pombe   515 GITCHGQCAHLIPDYCGMSN-----------DLKHQLLTELEVSKRPKKPELPN----------- 557

  Fly   219 IPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSRNDFMGALS----- 278
                   .:.||...|....|:.::. |:|..|                |.....:.|..
pombe   558 -------QENKTTNEKVYRKPLSSQN-TFDTLP----------------TISQGLLAATQPVTSV 598

  Fly   279 FGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKP---MVMRSDTNTHTSS 340
            ...|.:.|.|                       |:| ..|...||...|   .|:...::...||
pombe   599 LNTSPLPKTP-----------------------EKD-RSLNVTPSSSTPTPASVLAPPSSASLSS 639

  Fly   341 KKDMIRAT---------------DFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDD 390
            .||..|:.               ||.|:.|||||:||||:|||.|.:::.||||:|||:.|::::
pombe   640 SKDANRSVPESPRREKKNRVTLDDFTFLAVLGKGNFGKVMLAEYKVNKKFYAIKVLKKEAILKNE 704

  Fly   391 DVECTMIEKRV--LALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVF 453
            ::|....||.|  :|..||.|||:.|.:.|||..|::|||||:.|||||..||: .:|....|.|
pombe   705 ELESLKTEKHVFEVANKEKHPFLLNLFASFQTSTRVYFVMEYILGGDLMVHIQR-QQFSVKRARF 768

  Fly   454 YAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAP 518
            |.||:...|.:.|..||.||||||||:||..|||::|||:|:||||::...||.||||||:::||
pombe   769 YGAEVCLALKYFHENGIAYRDLKLDNILLCPDGHIRIADYGLCKENMLLGNTTSTFCGTPEFMAP 833

  Fly   519 EIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGF 583
            ||:|.|.|.|.|||||:|||:|:||:||.||.||||||:|.||......:|.::..:|....:|.
pombe   834 EILLEQQYSKDVDWWAFGVLMYQMLLGQSPFKGEDEEEIFDAILSDEPLFPINMPADAVSLLRGL 898

  Fly   584 LTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTD 648
            ||:.||:|||.|.....:|..||||..|.|:.:.|:..:|.:||.|...:|::|||::|||....
pombe   899 LTRDPNQRLGSGPKDANEVMAHPFFASIVWDDLYNKLYEPSYKPLINDPRDLNNFDEEFTSACPT 963

  Fly   649 LTPTDKVFMMNLDQSEFVGFS 669
            |||.:.| :....|..|.|||
pombe   964 LTPVNTV-LTRQQQECFRGFS 983

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkc53E	NP_725626.1	C1_cPKC_rpt1	44..110	CDD:410383	19/65 (29%)
		C1_cPKC_rpt2	120..173	CDD:410386	19/52 (37%)
		C2_PKC_alpha_gamma	177..307	CDD:175992	20/134 (15%)
		STKc_cPKC	353..672	CDD:270739	158/319 (50%)
pck1	NP_593737.2	HR1_PKC-like_1_fungi	2..73	CDD:212011
		HR1	111..180	CDD:294066
		C2	212..316	CDD:301316
		C1_1	414..462	CDD:278556	18/58 (31%)
		C1_1	481..533	CDD:278556	19/51 (37%)
		S_TKc	664..923	CDD:214567	135/259 (52%)
		STKc_PKC	668..983	CDD:270722	156/316 (49%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	46	1.000	Domainoid score	I3535
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	437	1.000	Inparanoid score	I315
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000117
OrthoInspector	1	1.000	-	-		mtm9272
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24356
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	1	1.000	-	-		X125
TreeFam	0	0.000	Not matched by this tool.
	9	9.090