DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pkc53E and Pkn2

DIOPT Version :9

Sequence 1:	NP_725626.1	Gene:	Pkc53E / 48311	FlyBaseID:	FBgn0003091	Length:	679	Species:	Drosophila melanogaster
Sequence 2:	NP_848769.2	Gene:	Pkn2 / 109333	MGIID:	109211	Length:	983	Species:	Mus musculus

Alignment Length:	541	Identity:	201/541 - (37%)
Similarity:	280/541 - (51%)	Gaps:	100/541 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly   174 TERRGRIYL---EINVKENLLT---------VQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQS 226
            ::::|:.:|   ::|:  |:.|         ..:.......|..|...:.|.|..: |||....:
Mouse   496 SKQQGKTFLRAPQMNI--NIATWGRLVRRAIPTVNHSGTFSPQTPVPATVPVVDAR-IPDLAPPA 557

  Fly   227 KKKTRTIKACLNPVWNETLTYDLKP----------------EDKDRRILIEVWDWDRTSRNDFMG 275
            ...|.|           .|.:||:|                |..:.|:|            |..|
Mouse   558 SDSTVT-----------KLDFDLEPEPPPAPPRASSLGETDESSELRVL------------DIPG 599

  Fly   276 ALSFGISEIIKNPTNGWFKLLTQDEGEY-----------YNVPCADDEQDLLKLKQKPSQKKPMV 329
            .   | ||.:.|..|....|..:.:.||           :.|...||::
Mouse   600 Q---G-SETVFNIENDRNNLRPKSKSEYELSIPDSGRSCWGVGELDDKR---------------- 644

  Fly   330 MRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVEC 394
                     :.::......||....|||:|.||||||||.|.:.|::|||.|||..|:..|:|:.
Mouse   645 ---------AQQRFQFSLQDFRCCAVLGRGHFGKVLLAEYKHTNEMFAIKALKKGDIVARDEVDS 700

  Fly   395 TMIEKRVLAL--GEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAE 457
            .|.|||:...  ..:.||||.|.:||||.:.:.|||||..|||||..|.. ..|.||.||||||.
Mouse   701 LMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHT-DVFSEPRAVFYAAC 764

  Fly   458 IAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENI-VGDKTTKTFCGTPDYIAPEII 521
            :..||.:||...|:||||||||:|||.:|.|||||||:|||.: .||:|: ||||||:::|||::
Mouse   765 VVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTS-TFCGTPEFLAPEVL 828

  Fly   522 LYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTK 586
            ....|.::||||..|||:||||||:.||.|:||||:|.:|.:..|.||:.||.||....:..|.:
Mouse   829 TETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRR 893

  Fly   587 QPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLT- 650
            .|.:|||.|....|||:.|||||..||..:.:::|:|||.|.|:.|:||||||.:||||...||
Mouse   894 NPERRLGAGEKDAEDVKKHPFFRLTDWSALMDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTP 958

  Fly   651 PTDKVFMMNLDQSEFVGFSYM 671
            |.:...::..:|..|..|.|:
Mouse   959 PREPRILLEEEQEMFHDFDYV 979

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkc53E	NP_725626.1	C1_cPKC_rpt1	44..110	CDD:410383
		C1_cPKC_rpt2	120..173	CDD:410386
		C2_PKC_alpha_gamma	177..307	CDD:175992	31/168 (18%)
		STKc_cPKC	353..672	CDD:270739	165/323 (51%)
Pkn2	NP_848769.2	HR1_PKN_1	42..107	CDD:212012
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	107..135
		HR1_PKN2_2	128..201	CDD:212021
		HR1_PKN2_3	209..282	CDD:212025
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	351..382
		Necessary to rescue apical junction formation	381..462
		C2_PKN-like	389..472	CDD:176069
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	553..588		6/45 (13%)
		STKc_PKN	656..981	CDD:270741	166/326 (51%)
		S_TKc	661..915	CDD:214567	136/255 (53%)
		Necessary for the catalytic activity. /evidence=ECO:0000250	916..976		25/59 (42%)
		Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoA. /evidence=ECO:0000250	977..983		1/3 (33%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167839288
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.870