DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pkc53E and prkceb

DIOPT Version :9

Sequence 1:	NP_725626.1	Gene:	Pkc53E / 48311	FlyBaseID:	FBgn0003091	Length:	679	Species:	Drosophila melanogaster
Sequence 2:	XP_002664164.2	Gene:	prkceb / 100332055	ZFINID:	ZDB-GENE-081028-47	Length:	738	Species:	Danio rerio

Alignment Length:	692	Identity:	310/692 - (44%)
Similarity:	415/692 - (59%)	Gaps:	109/692 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 DNNGDPQQQGA--EGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICG 68
            |.:|...:.|.  ..|.|...:|:.|.|:||:::: |..|..|.|:|.:.:|||:||||:|||
Zfish   130 DLSGSSSEAGGCENEERVFRERMRPRKRQGAVRRR-VHQVNGHKFMATYLRQPTYCSHCRDFI-- 191

  Fly    69 YQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDK--------GIDSDSPKTQHNFEPF 125
                  |...||||:|||||:.||||||||.:...|.|..|        |....|....|.|...
Zfish   192 ------WGVLGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQEDTAEEVGSQRFSVNMPHKFRIH 250

  Fly   126 TYAGPTFCDHCGSLLYGIYHQGLKCSACDMNVHARCKENVPSLCGCDHTERRG--RIYLEINVKE 188
            .|...||||||||||:|:..|||:|..|.||||.||:.||...||.|   .||  ::..::.|..
Zfish   251 NYKVLTFCDHCGSLLWGLLRQGLQCKVCKMNVHKRCEINVAPNCGVD---ARGIAKVLSDLGVTP 312

  Fly   189 NLLTVQIKEGRNL--------IPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETL 245
            :.|:..::..:.:        :|:.|           |:.:|:.:|...:
Zfish   313 DKLSGSVQRRKKISQGPDPHPLPISP-----------LLEEDRCKSAPTS--------------- 351

  Fly   246 TYDLKPEDKDRRILIEVWDWDRTSRNDFMGALSFG-ISEIIKNPTNGWFKLLTQDEGEYYNVPCA 309
                 |.|:|.:.|           .:...||||. .||.:|..:.|    ::::.||      |
Zfish   352 -----PCDQDIKDL-----------ENIRKALSFDHRSEEMKQASEG----VSEETGE------A 390

  Fly   310 DDEQDLLKLKQKPSQKKPMVMRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEE 374
            .:..::     |..|.|.|::.                 ||:|||||||||||||:|||.:|::|
Zfish   391 RENGEV-----KTVQAKRMMLE-----------------DFSFIKVLGKGSFGKVMLAELRGTDE 433

  Fly   375 LYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQ 439
            ::|:|:||||||:|||||:|||.|||:|||..|.|:|.||:.||||.||||||||||||||||||
Zfish   434 VFAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQ 498

  Fly   440 IQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDK 504
            ||:..||.|..:.|||||:.:.|.|||..|::||||||||:||||:||.|:||||||||.|:...
Zfish   499 IQRSRKFDEARSRFYAAEVTSALMFLHHNGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILDGI 563

  Fly   505 TTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYP 569
            ||.|||||||||||||:....||.||||||.|||:|||:.|||||:.::|::||.:|...:|.||
Zfish   564 TTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYP 628

  Fly   570 KSLSKEAKEACKGFLTKQPNKRLGC-GSSGEED-VRLHPFFRRIDWEKIENREVQPPFKPKIKHR 632
            ..|||||....|.|:||.|:||||| .|.|.|| :::||||:.|||..:|.|:|:|||||:||.:
Zfish   629 VWLSKEAVSILKAFMTKSPSKRLGCVVSQGLEDAIKVHPFFKDIDWVLLEQRKVKPPFKPRIKTK 693

  Fly   633 KDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPE 674
            :||:|||:.||.|:..|||.:...:..::|.||.||||...|
Zfish   694 RDVNNFDQDFTREEPVLTPVEDAIIKQINQEEFKGFSYFGEE 735

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkc53E	NP_725626.1	C1_cPKC_rpt1	44..110	CDD:410383	34/73 (47%)
		C1_cPKC_rpt2	120..173	CDD:410386	29/52 (56%)
		C2_PKC_alpha_gamma	177..307	CDD:175992	23/140 (16%)
		STKc_cPKC	353..672	CDD:270739	200/320 (63%)
prkceb	XP_002664164.2	C2_PKC_epsilon	3..135	CDD:175981	2/4 (50%)
		C1_1	171..221	CDD:278556	31/57 (54%)
		C1_1	245..297	CDD:278556	29/51 (57%)
		STKc_nPKC_epsilon	413..733	CDD:270743	199/319 (62%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170583311
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG51944
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000117
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	1	1.000	-	-		X125
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	7	6.880