DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pkc53E and prkcea

DIOPT Version :9

Sequence 1:	NP_725626.1	Gene:	Pkc53E / 48311	FlyBaseID:	FBgn0003091	Length:	679	Species:	Drosophila melanogaster
Sequence 2:	XP_005156350.1	Gene:	prkcea / 100005075	ZFINID:	ZDB-GENE-030131-8191	Length:	744	Species:	Danio rerio

Alignment Length:	668	Identity:	308/668 - (46%)
Similarity:	401/668 - (60%)	Gaps:	85/668 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 EAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGF 83
            |.|...:|:.|.|:||:::: |..|..|.|:|.:.:|||:||||:|||        |...||||:
Zfish   147 ERVFRERMRPRKRQGAVRRR-VHQVNGHKFMATYLRQPTYCSHCRDFI--------WGVLGKQGY 202

  Fly    84 QCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPK----------TQHNFEPFTYAGPTFCDHCGS 138
            |||||:.||||||||.:...|.|..|  ..|:|:          ..|.|....|..|||||||||
Zfish   203 QCQVCTCVVHKRCHELIITKCAGMKK--QEDTPEEVGSQRFSVNIPHMFSIHNYKVPTFCDHCGS 265

  Fly   139 LLYGIYHQGLKCSACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIP 203
            ||:|:..|||:|.||.||||.||:.||...||.| ..:..::..::.|..:.::...:..:.|.|
Zfish   266 LLWGLMRQGLQCKACKMNVHRRCESNVAPSCGVD-ARKIAKVLSDLGVTPDKISNSTQRRKKLTP 329

  Fly   204 MDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRT 268
            .     .||.......|..::..:.|:.....|                  |:..|.|:
Zfish   330 G-----QDPQQSTTEAPSSEESDRCKSAPTSPC------------------DQAELAEL------ 365

  Fly   269 SRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSD 333
              .....|||||......:.::......|:.||.        :..||     |..|.|.|.:
Zfish   366 --ESIRKALSFGQEHKSSSSSSCSGVQSTEIEGR--------ENGDL-----KTVQTKRMSL--- 412

  Fly   334 TNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIE 398
                          |||:|||||||||||||:|||.||::|:||:|:||||||:|||||:||:.|
Zfish   413 --------------TDFSFIKVLGKGSFGKVMLAELKGTDEVYAVKVLKKDVILQDDDVDCTLTE 463

  Fly   399 KRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLF 463
            ||:|||..|.|:|.||..||||.||||||||||||||||||||:..||.|..:.|||||:.:.|.
Zfish   464 KRILALARKHPYLTQLFCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEARSRFYAAEVTSALM 528

  Fly   464 FLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGK 528
            |||..|::||||||||:||||:||.|:||||||||.|:...||.|||||||||||||:....||.
Zfish   529 FLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGP 593

  Fly   529 SVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPNKRLG 593
            ||||||.|||:|||:.|||||:.::|::||.:|...:|.||..|||||....|.|:||.|:||||
Zfish   594 SVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPSKRLG 658

  Fly   594 C-GSSG-EEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVF 656
            | .:.| ||.:::|||||.|||.::|.|.::|||||:||.::||:|||:.||.|...|||.|...
Zfish   659 CVVTQGLEEAIKVHPFFREIDWVQLEQRRIKPPFKPRIKTKRDVNNFDQDFTREDPVLTPVDDAI 723

  Fly   657 MMNLDQSEFVGFSYMNPE 674
            :..::|.||.||||...|
Zfish   724 IKQINQEEFKGFSYFGEE 741

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkc53E	NP_725626.1	C1_cPKC_rpt1	44..110	CDD:410383	33/65 (51%)
		C1_cPKC_rpt2	120..173	CDD:410386	31/52 (60%)
		C2_PKC_alpha_gamma	177..307	CDD:175992	19/129 (15%)
		STKc_cPKC	353..672	CDD:270739	201/320 (63%)
prkcea	XP_005156350.1	C2_PKC_epsilon	3..135	CDD:175981
		C1_1	173..223	CDD:278556	31/57 (54%)
		C1_1	247..299	CDD:278556	31/51 (61%)
		S_TKc	415..675	CDD:214567	169/259 (65%)
		STKc_nPKC_epsilon	419..739	CDD:270743	200/319 (63%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170583312
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG51944
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000117
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	1	1.000	-	-		X125
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	7	6.880