DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mus101 and Topbp1

DIOPT Version :9

Sequence 1:	NP_524909.1	Gene:	mus101 / 48309	FlyBaseID:	FBgn0002878	Length:	1425	Species:	Drosophila melanogaster
Sequence 2:	XP_006511769.3	Gene:	Topbp1 / 235559	MGIID:	1920018	Length:	1541	Species:	Mus musculus

Alignment Length:	1595	Identity:	362/1595 - (22%)
Similarity:	595/1595 - (37%)	Gaps:	418/1595 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    26 VQEADTLQQFEAARELLGQQLAETQIRQIKPSEGYPLIAAGNLTKKDVFVLTQFEGEFFEQLQQT 90
            ::.:|..:.|..|.|.:.:..:|..::.|...|...:    ....|.:::..:|.|..|:.|:|.
Mouse    89 LKSSDNSECFFKALESIKELQSEDYLQIITDEEALKI----RENDKSLYICDRFSGTVFDHLKQL 149

  Fly    91 RALILGPPCLITCLRRNEPIPEGSSAIYSTAMRDLQVSATGITPQKKEELSRLINWMGGIYFQSF 155
            ...|:||..:..|:|..:.:|.....:|:..|.|:.||.|.:...|:||:.:.:..|||..::..
Mouse   150 GCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDL 214

  Fly   156 GHRTTHLISNTIKSSKYEQATLNGVPVMHVDWVQYVWDQSRRSQREGIMATDPDFDKYRLPIFFG 220
            ....||||:..:.|.||..|.....|::...|::.:|::|:  :::....||.:.:.::.|||.|
Mouse   215 NVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQ--EKKITKYTDVNMEDFKCPIFLG 277

  Fly   221 ANITCSGLDVARKDQVMRLVNDNGGIYHRAFRSQVVDIVITEQTKTDTEKYKAAIRYKKDVLLPE 285
            ..|..:||:...:..|.:|...:||.|....:......:|.::.|  .:||:.|.|:....:..:
Mouse   278 CIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPK--GQKYECARRWNVHCVTLQ 340

  Fly   286 WIFDSCNRGYALPTKDYEVRPGKTSSTPTKTTRPGAAPGADQTHLSDLSRISFVSGSRRMCSDLS 350
            |..||..:|:......|:......:.....|:.|.|...|:...|:|:|.||.::||   |
Mouse   341 WFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSNAESHTLADVSHISNINGS---C---- 398

  Fly   351 TVNESVSSVGSSSPAKQLLKQATSSGRNYQQVLAEIEPRQAKKAGAFLDGCCVYLSGFRSEEREK 415
             |||::  .||::      .:...|..|.:.:  :|...||.:  ..||||.:||.||...:.:|
Mouse   399 -VNETM--FGSTT------SKLECSLENLENL--DISMFQAPE--DLLDGCRIYLCGFSGRKLDK 450

  Fly   416 LNRVLNTGGATRYDEANEGISHIIVGQLDDAEYRQWQRDGLMGSVHVVRLDWLLESIRAGRVVSE 480
            |.|::|:||..|:::.||.::|:|||..||...:.|.:..  ...|||...||||....|.::.|
Mouse   451 LRRLINSGGGVRFNQLNEDVTHVIVGDYDDDVRQFWSKSS--HRPHVVGAKWLLECFTKGYILPE 513

  Fly   481 --LVHRVSMPQNREP-DVA---SPASKRTLRSMNHSFKQPTLPIKKKLFDQEPDPVQEQEHEEPD 539
              .:|     .|.:| .:|   .|.::..:...:.||.:..|             |..:..::.|
Mouse   514 ESYIH-----TNYQPAGIAVSDQPGNQTAVLDKSGSFSKSAL-------------VPAERLQQAD 560

  Fly   540 HTLLDQYSQDQGAVAQL-----------PPADVSLLQPAASSTQMDIRQRVSVANPKPPAEGLQL 593
            ..||.||..|...:.:.           |....:..:|...||.:.:::     ..|.......|
Mouse   561 EDLLAQYGNDDSTMVEAKLSEALEPEVGPCPGSAHREPCDDSTHISVQE-----ENKSSVSHCIL 620

  Fly   594 PDLSASTLSIDFDKLDYFAGVSVYVHRECFNEEFFNQMLTECEAAQGLLVPSSFSDEVDFAIVSF 658
            .|.:.....:...|.....|.|  |..:|...:...:     .|.:.:.:||..  ..|:|:|..
Mouse   621 DDSTVREEGLFSQKSFLVLGFS--VENKCNIVDIIRE-----HAGKIVSLPSRI--VADYAVVPL 676

  Fly   659 EVAFDVKQLPVKARHVVTELFLESCMKKNQLLPIEYYHKHVPATALR--QPLKGMTIVVSIYAGL 721
            ...    ::.|....|||..:|.:|:....|:..:......|.:.:.  .||:...|..|...|.
Mouse   677 LGC----EVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTPLEDCVISFSQCVGA 737

  Fly   722 ERDFINATAELLGASVNKTFIKK---EKPL-----LVCPSAEGSKYEGAIKWNYPVVTSDWLVQC 778
            |||.:...|..|||||.:.|::|   :|.:     |:.....|||||.|.||:.|.|...||::.
Mouse   738 ERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLET 802

  Fly   779 ARTGQKLPFVGYLVGKSPE-----------------DFPISPRLR------------DSN---SR 811
            ||.|::.....:||..:|:                 |.|..|...            |.|   ||
Mouse   803 ARIGKRADENHFLVDNAPKQEQVLETKIPNGVSSNPDLPAHPDAHLEIHRKKAVTPLDMNRFQSR 867

  Fly   812 TARR-------------PNESTLVAQPDVTMEEAEN--------QPAGSVTPVTA---------- 845
            ..|.             |....|..:|.:.::....        :|:..||...|
Mouse   868 AFRAVISQQRGQDPTFPPVRQPLTKEPSLHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQ 932

  Fly   846 ----GSP------------------------GAPEL-----------TPL----------RNKRV 861
                .||                        .:|:|           .||          .:|:.
Mouse   933 KRKLSSPLSEVIVRNLTVALANSSRNTDSHSASPQLKGAHLEEEETRKPLDSVVVCVSKKLSKKQ 997

  Fly   862 SELAGIPGG------------------------------SALHRGSNSTS-----SPDSPCTPLS 891
            |||.|:...                              ||..||.:..|     .||.    ||
Mouse   998 SELNGVAASLGAEYRWSFDETVTHFIYQGRANDSNREYKSAKERGVHIVSEHWLLEPDH----LS 1058

  Fly   892 QVGAQQYNLDFLEQFVQRLDTEEGKDCVREIIR--EMRENQTPELERIRRQAC---TPVSRKHQR 951
            ..|.:...:...::....|:.....||...:.:  |||||...:|:.|....|   ||..:.
Mouse  1059 VEGNETNTMGTNDKESPLLNGSGRDDCKGALTQALEMRENFQKQLQEIMSATCIVKTPAQKT--- 1120

  Fly   952 PAPGIPDFCLTPEFQQRMADDFERRWRLPTMKIKPDTPLAVI--RQRVMRITCETLGIEYEESNA 1014
                    |::             |....:....||:..:|.  |.||:    |.|    .:|..
Mouse  1121 --------CMS-------------RSSCNSASSTPDSARSVRSGRSRVL----EAL----RQSRQ 1156

  Fly  1015 KTPTLSESPSTVKK---KPPT----RTTQATKLNFDRSP----------KTPKLSLGKKTPLRVS 1062
            ..|.::..||..::   ..||    |...|:.|.:...|          |.|..|..:|......
Mouse  1157 AVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSDPTQHSELQVEIKMPDDSPSRKPVYHSE 1221

  Fly  1063 MGSPRS-GTQSPFVPNTQSPIEAAPPRRSDGPTLSEEGQSTINFDKISFEESAVPVLAPDVPTVA 1126
            :....| .||:|..|.::.|    .|..::.|.:.|                      |..|.||
Mouse  1222 IAEQASCVTQAPGHPGSEEP----EPPVAERPLIPE----------------------PQAPAVA 1260

  Fly  1127 ------PDVKQITDYLKNCESRRNSLKRSHDNDMDCGESEVQYVQPFESEGFALGTEDMVDWRDP 1185
                  |...|..|.::..|.....:|:             |||....|    |.:::.:|:
Mouse  1261 SPLAKPPVAPQPADKIETQEETHRKVKK-------------QYVFQMSS----LNSQERIDY--- 1305

  Fly  1186 AEFNAAKRRSSGGSPKMQYAGIPCFSISCGDDDEKRAELIARITQLGGKVCENLVNYDDSCTHLL 1250
                                        |           ..|..|||.|.|...: |.||||::
Mouse  1306 ----------------------------C-----------RLIKDLGGSVIEKQCS-DPSCTHMV 1330

  Fly  1251 CERPNRGEKMLACIAAGKWILNIQYIEQSHARGDFLDETLYEWGNPKAIN-LPTLAPEEEPIAAA 1314
            ...|.|.||.||.:|||||:|:..|::.....|.|:.|..||||:...:: ||.:...::.:|.|
Mouse  1331 VGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALA 1395

  Fly  1315 VHRWRTEL-----SACGGGAFSDHRVILSMNERSGAPIRNVLRAGGACILEPTTPFSKDPVAKSA 1374
            ..|||..:     |....||||..:.||.::....|..:.:|:||||.:|..    ..:|:.|.|
Mouse  1396 AMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLSG----HPEPLLKDA 1456

  Fly  1375 SHCFVDVKK----------APLSTQDM-----EYL 1394
            :|.|.|..|          |..:.|:|     ||:
Mouse  1457 THLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYI 1491

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mus101	NP_524909.1	BRCT	118..188	CDD:278934	21/69 (30%)
		BRCT	216..290	CDD:278934	19/73 (26%)
		BRCT	397..472	CDD:278934	31/74 (42%)
		BRCT	707..778	CDD:278934	30/78 (38%)
		BRCT	1210..1279	CDD:237994	25/68 (37%)
Topbp1	XP_006511769.3	BRCT_TopBP1_rpt1	183..254	CDD:349369	21/70 (30%)
		BRCT_TopBP1_rpt2_like	273..349	CDD:349363	21/77 (27%)
		BRCT_TopBP1_rpt3	427..508	CDD:349350	33/84 (39%)
		BRCT_TopBP1_rpt4	631..714	CDD:349380	18/95 (19%)
		BRCT_TopBP1_rpt5	723..811	CDD:349387	33/87 (38%)
		BRCT_TopBP1_rpt6	982..1052	CDD:349359	11/69 (16%)
		Streccoc_I_II	<1159..>1272	CDD:411384	27/138 (20%)
		PHA01732	1228..>1297	CDD:222828	21/111 (19%)
		BRCT_TopBP1_rpt7	1289..1363	CDD:349370	31/120 (26%)
		BRCT_TopBP1_rpt8	1418..1496	CDD:349360	21/78 (27%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	69	1.000	Domainoid score	I9643
eggNOG	1	0.900	-	-		E1_KOG1929
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	333	1.000	Inparanoid score	I2404
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48684
OrthoDB	1	1.010	-	-		D305707at33208
OrthoFinder	1	1.000	-	-		FOG0003632
OrthoInspector	1	1.000	-	-		oto95077
orthoMCL	1	0.900	-	-		OOG6_102493
Panther	1	1.100	-	-	LDO	PTHR13561
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1061
SonicParanoid	1	1.000	-	-		X5299
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.870