Protein Alignment aPKC and PKN2

DIOPT Version :10

• Sequence 1: NP_001260984.1
• Gene: aPKC / 47594
• FlyBaseID: FBgn0261854
• Length: 958
• Species: Drosophila melanogaster
• Sequence 2: NP_006247.1
• Gene: PKN2 / 5586
• HGNCID: 9406
• Length: 984
• Species: Homo sapiens
• Alignment Length: 448
• Identity: 174/448 - (38%)
• Similarity: 241/448 - (53%)
• Gaps: 65/448 - (14%)

  Fly   553 PLVKERAEESSDPI-------------PVPLPPLPYEAMSGG--------------AEACET--- 587
            |:|..|..:.:.|.             |.| ||.|..|.|.|              .:..||
Human   545 PVVDVRIPQLAPPASDSTVTKLDFDLEPEP-PPAPPRASSLGEIDESSELRVLDIPGQDSETVFD 608

  Fly   588 --HDHAHIV-------APPPPEDPLE-PGTQR----------QYSLNDFELIRVIGRGSYAKVLM 632
              :|...|:       .|..|:..|| .|.|.          |::|.||....|:|||.:.|||:
Human   609 IQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLL 673

  Fly   633 VELRRTRRIYAMKVIKKALVTDDEDIDWVQTEKHVFETASN--HPFLVGLHSCFQTPSRLFFVIE 695
            .|.:.|..::|:|.:||..:...:::|.:..||.:|||.::  |||||.|.:||||...:.||:|
Human   674 AEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVME 738

  Fly   696 FVRGGDLMYHMQRQRRLPEEHARFYAAEISLALNFLHEKGIIYRDLKLDNVLLDHEGHIKLTDYG 760
            :..|||||.|:... ...|..|.||||.:.|.|.:|||..|:||||||||:|||.||.:|:.|:|
Human   739 YAAGGDLMMHIHTD-VFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFG 802

  Fly   761 MCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLLYEMLAGRSPFDLAGASENPD 825
            :||||:..||.||||||||.::|||:|....|..:||||.||||:||||.|.|||        |.
Human   803 LCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPF--------PG 859

  Fly   826 QNTEDYLFQVILEKTIRIPRSLSVRAASVLKGFLNKNPADRLGCHRESAFMDIVSHPFFKNMDWE 890
            .:.|: :|..|:...:|.||.||..|.|:::..|.:||..|||...:.| .|:..||||:.:||.
Human   860 DDEEE-VFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDA-EDVKKHPFFRLIDWS 922

  Fly   891 LLERKQVTPPFKPRLDSDRDLANFPPEFTGEAVQLTPD-DDHVIDNIDQSEFEGFEYV 947
            .|..|:|.|||.|.:....|::||..|||.||..|||. :..::...:|..|..|:|:
Human   923 ALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYI 980

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
aPKC	NP_001260984.1	C1_aPKC	496..550	CDD:410344
		STKc_aPKC	620..947	CDD:270740	147/329 (45%)
PKN2	NP_006247.1	HR1_PKN_1	42..107	CDD:212012
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	114..133
		HR1	130..201	CDD:469609
		HR1_PKN2_3	209..282	CDD:212025
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	351..383
		Necessary to rescue apical junction formation. /evidence=ECO:0000250	382..463
		C2_PKN-like	390..473	CDD:176069
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	558..584		6/26 (23%)
		STKc_PKN	657..982	CDD:270741	149/335 (44%)
		Necessary for the catalytic activity	917..977		22/59 (37%)
		Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoA	978..984		1/3 (33%)