DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-zeta and Pola1

DIOPT Version :9

Sequence 1:	NP_524881.2	Gene:	DNApol-zeta / 47186	FlyBaseID:	FBgn0002891	Length:	2130	Species:	Drosophila melanogaster
Sequence 2:	NP_445931.1	Gene:	Pola1 / 85241	RGDID:	621816	Length:	1468	Species:	Rattus norvegicus

Alignment Length:	1564	Identity:	296/1564 - (18%)
Similarity:	545/1564 - (34%)	Gaps:	437/1564 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly   767 DEC---SSEIQTTGPRVTTTS-LDRPQKKARLSQSPKENTKTSMNG----------TVALEKATK 817
            |:|   :|.:..:|..|.:.: .::..||.|  |...|..|.:..|          |...|:..:
  Rat     7 DDCKLETSAVSDSGSFVASRARREKKSKKGR--QEALERLKKAKAGEKYKYEVEDLTSVYEEVDE 69

  Fly   818 DSSSNSESPHQQENSV--SEQIEYLES-----KPKKSDETARSCDE--------KLQRELIPQEP 867
            :..|......|.::.:  .:.|.|:|.     .....|:...:|.|        |.::::  ::|
  Rat    70 EQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGEGSDGKAHRKDRKDV--KKP 132

  Fly   868 AGISPGD--------SANSTEEITFSPCHDEAI-----ESDTESDYI------VTKLRKTPNLKR 913
            :...|.:        :...|.:.|.....|:.:     :.:||:..|      :.|.:::.....
  Rat   133 SVTKPNNIKAMFIASAGKKTTDKTVDLSKDDLLGDILQDLNTETPQIAPPPVLIPKKKRSTGASP 197

  Fly   914 LRWSIRSELLNKQFTPSSGIRPPETETTPQLSPK-------SNESNTPELMRSFYEHSLIVNSPS 971
            ..:|:.:...    .||..|..|.:...|.|:|.       :.:...||......|..:|.....
  Rat   198 NPFSVHTATA----VPSGKIASPVSRKEPPLTPVPLKRAEFAGDLAQPECPEDEQESGVIEFEDG 258

  Fly   972 VFSDFLDSPEIHMDSPRSAPPSPDSNSFVIAPLELPPSYDEVVSGSRKMDIPE---YEFQKPYYS 1033
            .|.:.:|:.|:..:.|.:|              ::.....|.|.|.:....||   ..|...:..
  Rat   259 DFDEPMDTEEVDEEEPVTA--------------KIWDQESEPVEGVKHEADPETGTTSFLDSFLP 309

  Fly  1034 NPS--DVSKVTEVGFLVLHIPGNKLNDCDPFQSILGNDRGLASWRRRQLIAIGGLAMLQRHRGEQ 1096
            :.|  |:.:..|..||:..:.    .|.:....:.|.|                         ::
  Rat   310 DVSCWDIDQKDENSFLLQEVQ----VDSNHLPLVKGAD-------------------------DE 345

  Fly  1097 KVREYFSTQQRIAIEPAQLAPTWQEA---------------KIWLKAKE-------LLRQREE-- 1137
            :|.:::                |.:|               |:|:::.:       :::..|.
  Rat   346 QVFQFY----------------WLDAYEDPYNQPGVVFLFGKVWVESAKTHVSCCVMVKNIERTL 394

  Fly  1138 ---PKKSSDDIDSPIKIKRQKITMMLQAEEGDGGSGDEDAGEELDCSLSLTPLSQAKDKCKATPT 1199
               |::...|:::. |.....|||             :|..||.|..:|.        |.|....
  Rat   395 YFLPREMKIDLNTG-KETATPITM-------------KDVYEEFDSKISA--------KYKIMKF 437

  Fly  1200 SSKARETGKSRLKRGTRLSFIGSQDEEPPSSQSSEQSVSSSAAQAELDRSSFLRQLEGSSQDRQH 1264
            .||..|.           ::.....:.|..|:..|...|:...|..       :.|:|.:
  Rat   438 KSKIVEK-----------NYAFEIPDVPEKSEYLEVRYSAEVPQLP-------QNLKGET----- 479

  Fly  1265 DLSFGLSHATLDNTFGFK-------------VNLENLQQAKADID-CNHLTIITLEVFVSTRGDL 1315
                 .||....||...:             :.::|.|.....|. |....:......|:...|:
  Rat   480 -----FSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEAMALKPDLVNVIKDV 539

  Fly  1316 QPDPM----------------HDEIRCLFYAIEHSLPDEKLPSK---ACGYIMVNTVQDLLSEGP 1361
            .|.|:                ..||..:...:.|:.|.:|.|.|   ...:.:|:..:|.:....
  Rat   540 SPPPLVVMSFSMKTMQNVQNHQHEIIAMAALVHHNFPLDKAPPKPPFQTHFCVVSKPKDCIFPCA 604

  Fly  1362 FHGI--DRDIEVQVVTSEAEAFEALLALCERWDADIYAGYEIEMSSWGYVIDRAKHLC-FNIAPL 1423
            |..:  .:::||:|..:|.......||...:.|.||..|:.|               | |.:..|
  Rat   605 FKEVIKKKNMEVEVAATERTLLGFFLAKVHKLDPDILVGHNI---------------CGFELEVL 654

  Fly  1424 LSRVPTQKVRDFVDEDREQFTDLDVEMKL------------CGRILLDVWRLMRSEIALTSYTFE 1476
            |.|:...||..:....|.:.:::.   ||            |||::.||...::..|...||...
  Rat   655 LQRINECKVPFWSKIGRLRRSNMP---KLGSRSGFGERNATCGRMICDVEISVKELIHCKSYHLS 716

  Fly  1477 NVMYHILHKRCPWHTAKSLTEWFGSPCTRWIVMEYYLERVRGTLTLLDQLDLLGRTSEMAKLIGI 1541
            .::..||.........:::...:..|.....::|:..:..|..|.::.:|::|....::..:.|.
  Rat   717 ELVQQILKTERIVIPTENIRNMYSEPSHLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGN 781

  Fly  1542 QFYEVLSRGSQFRVESMMLRIAKPKNLVPLSPSVQARAHMRAPEYL------------------- 1587
            .....|..|...|.|.::|......|.:     |..:...|.|:..
  Rat   782 IMSRTLMGGRSERNEFLLLHAFYENNYI-----VPDKQIFRKPQQKPGDEDEEIDGDTNKYKKGR 841

  Fly  1588 -------ALIMEPQSRFYADPLIVLDFQSLYPSMIIAYNYCFSTCLGRVEHLGGSSPFEFGASQL 1645
                   .|:::|:..||...:::|||.|||||:|..:|.||:|.                   .
  Rat   842 KKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTV-------------------Q 887

  Fly  1646 RVSRQMLQKLLEHDLVTVSPCGVVFVKREVRE--------GILPRMLTEILDTRQMVKQSMKLHK 1702
            ||:.:.|:...:.:            :.::.|        |||||.:.::::.|:.|||.||...
  Rat   888 RVASETLKATEDEE------------QEQIPELPDPNLDMGILPREIRKLVERRKQVKQLMKQQD 940

  Fly  1703 DSSALQRILHSRQLGLKLMANVTYGYTAANFSGRMPSVEVGDSVVSKGRETLERAIKLVENNEEW 1767
            .:..|......||..|||.||..||....::| |..:..:...|..||||.|....::|   ::.
  Rat   941 LNPDLVLQYDIRQKALKLTANSMYGCLGFSYS-RFYAKPLAALVTYKGREILMHTKEMV---QKM 1001

  Fly  1768 KVRVVYGDTDSMFVLVPGRNRAEAFRIGEEIAKAVTEMNPQPVKLKLEKVYQPCMLQTKKRYVGY 1832
            .:.|:||||||:.:.....|..|.|::|.::...|.::. :.:::.::.|::..:|..||:|...
  Rat  1002 NLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKNEVNKLY-KLLEIDIDGVFKSLLLLKKKKYAAL 1065

  Fly  1833 MYETADQEQPV--YEAKGIETVRRDGCPAVAKMLEKVLRILFETQDVSKIKA-YVCRQFTKLLSG 1894
            :.|.......:  .|.||::.||||.|                  |::|... :|..|   :||.
  Rat  1066 VVEPTSDGNYITKQELKGLDIVRRDWC------------------DLAKDTGNFVIGQ---ILSD 1109

  Fly  1895 RA------NLQDLIFAKEFRGLNGYKPTACVPALELTRKWMQKDPRHVP---------------- 1937
            ::      |:|..:.......|||..|   |...|: .|.:.|||:..|
  Rat  1110 QSRDTIVENIQKRLIEIGENVLNGSVP---VSQFEI-NKALTKDPQDYPDKKSLPHVHVALWINS 1170

  Fly  1938 ------RRGERVPFIIVNGPPGMQLIRLVRSPHDILANEGHKINAIYYITKAIIPPLNR-C---- 1991
                  :.|:.|.::|......:...:...:|..:...:...|:..||:.:.|.|.:.| |
  Rat  1171 QGGRKVKAGDTVSYVICQDGSNLPATQRAYAPEQLQKQDNLAIDTQYYLAQQIHPVVARICEPID 1235

  Fly  1992 -------LLLIGAN-----VHDWFASLPR-KLLMTPAVGTANE----------LAGARGAKSTIS 2033
                   .|.:|.:     ||.:...... .||..||..|..|          |..:.|.::...
  Rat  1236 GIDAVLIALWLGLDSTQFRVHQYHKDEENDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYD 1300

  Fly  2034 QYFSTTSCVIDCGRQTKAGICPDCLKNATTCVVVLSDK----TARLERGYQLTRQICQ--ACCGR 2092
            ..|..:...::......:.|  ||..:..|.:|.||:|    ..|..:.|.....||:  .|..|
  Rat  1301 NVFEGSGMDMEPSLNRCSNI--DCKASPATFMVQLSNKLIMDIRRCIKKYYDGWLICEEPTCRNR 1363

  Fly  2093 LGSL 2096
            :..|
  Rat  1364 IRRL 1367

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-zeta	NP_524881.2	PTZ00166	20..>196	CDD:240301
		DNA_polB_zeta_exo	1297..1518	CDD:99821	48/254 (19%)
		POLBc_zeta	1550..2000	CDD:99917	117/531 (22%)
		zf-C4pol	2041..2107	CDD:405022	15/62 (24%)
Pola1	NP_445931.1	DNA_pol_alpha_N	41..101	CDD:289058	10/59 (17%)
		pol2	56..1232	CDD:273159	255/1374 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	105..135		5/31 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	174..226		11/55 (20%)
		DNA_polB_alpha_exo	542..774	CDD:99819	48/249 (19%)
		DNA-binding. /evidence=ECO:0000255	657..722		14/67 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	816..836		2/19 (11%)
		POLBc_alpha	839..1249	CDD:99915	109/470 (23%)
		DNA-binding. /evidence=ECO:0000255	1252..1383		26/118 (22%)
		zf-DNA_Pol	1272..1461	CDD:286132	21/98 (21%)
		CysB motif	1355..1381		4/13 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810