DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-zeta and Pola1

DIOPT Version :9

Sequence 1:	NP_524881.2	Gene:	DNApol-zeta / 47186	FlyBaseID:	FBgn0002891	Length:	2130	Species:	Drosophila melanogaster
Sequence 2:	NP_032918.1	Gene:	Pola1 / 18968	MGIID:	99660	Length:	1465	Species:	Mus musculus

Alignment Length:	1571	Identity:	307/1571 - (19%)
Similarity:	537/1571 - (34%)	Gaps:	470/1571 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   800 ENTKTSMNGTVALEKATKDSSSNSESPHQQENSVSEQIEYLES-------KPKKSDETA--RSCD 855
            |.:..|.:|:.|..:|.::..|        :....|.:|.|:.       |.:..|.|:  ...|
Mouse    12 EASAVSDSGSFAASRARREKKS--------KKGRQEALERLKKAKAGEKYKYEVEDLTSVYEEVD 68

  Fly   856 EKLQRELIP--QEPAGISPGDSANSTE---EITFSPCHDEAIE-----SDTES------DYIVTK 904
            |:...:|:.  |:...|...|.....|   ||......|:|::     ||.::      |.....
Mouse    69 EEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGKGSDGKAHRKDRKDVKKPS 133

  Fly   905 LRKTPNLKRL-----------------RWSIRSELLNKQFTPSSGIRPPETETTPQLSPKSNESN 952
            :.|..|:|.:                 :..:..::|....|.::.|.||     |.|.||...|
Mouse   134 VTKPNNIKAMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTETAQITPP-----PVLIPKKKRS- 192

  Fly   953 TPELMRSFYEHSLIVNSPSVFSDFLDSPEIHMDSPRSAPPSPD-SNSFVIAPLELPPSYDEVVSG 1016
            |..|:..|..|     :|...             |...|.||. .|..::.|:.|..:  |:...
Mouse   193 TGALLNPFSVH-----TPKAI-------------PSGKPASPVLRNEPLLTPIPLKRA--ELAGE 237

  Fly  1017 SRKMDIPE-------YEFQKPYYSNPSDVSKV-------------TEVGFLVLHIPGNKLNDCDP 1061
            ..:.:.||       .||:...:....|..||             ||....|.|       :.||
Mouse   238 LAQPECPEDEQELGVMEFEDGDFDESMDTEKVDEKPVTAKTWDQETEPVERVEH-------EADP 295

  Fly  1062 FQSILGNDRGLASWRRRQLIAIGGLAMLQRHRGEQKVREYFSTQQRIAIEPAQLAPT-------- 1118
                   :||..|:....|..:....:.|...         |..|.:.::.:.|...
Mouse   296 -------ERGTTSYLENFLPDVSCWDIDQDDE---------SIPQEVQVDSSNLPLVKGADDEQV 344

  Fly  1119 ----WQEA---------------KIWLKAKE-------LLRQREE-----PKKSSDDIDS----- 1147
                |.:|               |:|:::.:       :::..|.     |::...|:::
Mouse   345 FQFYWLDAYEDPYNQPGVVFLFGKVWIESVKTHVSCCVMVKNIERTLYFLPREMKFDLNTGKETA 409

  Fly  1148 -PIKIKRQKITMMLQAEEGDGGSGDEDAGEELDCSLSLTPLSQAKDKCKATPTSSKARETGKSRL 1211
             |:.:|                    |..||.|..:|.        |.|.....||..|.
Mouse   410 IPVTMK--------------------DVYEEFDSKISA--------KYKIMKFKSKIVEK----- 441

  Fly  1212 KRGTRLSFIGSQDEEPPSSQSSEQSVSSSAAQAELDRSSFLRQLEGSSQDRQHDLSFGLSHATLD 1276
                  ::.....:.|..|:..|...|:...|..       :.|:|.:          .||....
Mouse   442 ------NYAFEIPDVPEKSEYLEVRYSAEVPQLP-------QNLKGET----------FSHVFGT 483

  Fly  1277 NTFGFK-------------VNLENLQQAKADIDCNHLTIITLEV-FVSTRGDLQPDPM------- 1320
            ||...:             :.::|.|.....|......::.|:. .|:...|:.|.|:
Mouse   484 NTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEVMALKPDLVNVIKDVSPPPLVVMSFSM 548

  Fly  1321 ---------HDEIRCLFYAIEHSLPDEKLPSK---ACGYIMVNTVQDLLSEGPFHGI--DRDIEV 1371
                     ..||..:...:.||...:|.|.:   ...:.:|:..:|.:....|..:  .::::|
Mouse   549 KTMQNVQNHQHEIIAMAALVHHSFALDKAPPEPPFQTHFCVVSKPKDCIFPCDFKEVISKKNMKV 613

  Fly  1372 QVVTSEAEAFEALLALCERWDADIYAGYEIEMSSWGYVIDRAKHLC-FNIAPLLSRVPTQKVRDF 1435
            ::..:|.......||...:.|.||..|:.|               | |.:..||.|:...||..:
Mouse   614 EIAATERTLIGFFLAKVHKIDPDILVGHNI---------------CSFELEVLLQRINECKVPYW 663

  Fly  1436 VDEDREQFTDLDVEMKL------------CGRILLDVWRLMRSEIALTSYTFENVMYHILHKRCP 1488
            ....|.:.:::.   ||            |||::.||....:..|...||....::..||.....
Mouse   664 SKIGRLRRSNMP---KLGSRSGFGERNATCGRMICDVEISAKELIHCKSYHLSELVQQILKTERI 725

  Fly  1489 WHTAKSLTEWFGSPCTRWIVMEYYLERVRGTLTLLDQLDLLGRTSEMAKLIGIQFYEVLSRGSQF 1553
            ....:::...:........::|:..:..|..|.::.:|::|....::..:.|......|..|...
Mouse   726 VIPTENIRNMYSESSYLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSE 790

  Fly  1554 RVESMMLRIAKPKNLVPLSPSVQARAHMRAPEYL--------------------------ALIME 1592
            |.|.::|......|.:     |..:...|.|:..                          .|:::
Mouse   791 RNEFLLLHAFYENNYI-----VPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKATYAGGLVLD 850

  Fly  1593 PQSRFYADPLIVLDFQSLYPSMIIAYNYCFSTCLGRVEHLGGSSPFEFGASQLRVSRQMLQKLLE 1657
            |:..||...:::|||.|||||:|..:|.||:|.                   .||:.: :||..|
Mouse   851 PKVGFYDKFILLLDFNSLYPSIIQEFNICFTTV-------------------QRVTSE-VQKATE 895

  Fly  1658 HDLVTVSPCGVVFVKREVRE--------GILPRMLTEILDTRQMVKQSMKLHKDSSALQRILHSR 1714
            .:           .:.::.|        |||||.:.::::.|:.|||.||....:..|......|
Mouse   896 DE-----------EQEQIPELPDPNLEMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIR 949

  Fly  1715 QLGLKLMANVTYGYTAANFSGRMPSVEVGDSVVSKGRETLERAIKLVENNEEWKVRVVYGDTDSM 1779
            |..|||.||..||....::| |..:..:...|..||||.|.....:|   ::..:.|:||||||:
Mouse   950 QKALKLTANSMYGCLGFSYS-RFYAKPLAALVTYKGREILMHTKDMV---QKMNLEVIYGDTDSI 1010

  Fly  1780 FVLVPGRNRAEAFRIGEEIAKAVTEMNPQPVKLKLEKVYQPCMLQTKKRYVGYMYETADQEQPV- 1843
            .:.....|..|.|::|.::...|.::. :.:::.::.|::..:|..||:|...:.|.......:
Mouse  1011 MINTNSTNLEEVFKLGNKVKSEVNKLY-KLLEIDIDAVFKSLLLLKKKKYAALVVEPTSDGNYIT 1074

  Fly  1844 -YEAKGIETVRRDGCPAVAKMLEKVLRILFETQDVSKIKA-YVCRQFTKLLSGRA------NLQD 1900
             .|.||::.||||.|                  |::|... :|..|   :||.::      |:|.
Mouse  1075 KQELKGLDIVRRDWC------------------DLAKDTGNFVIGQ---ILSDQSRDTIVENIQK 1118

  Fly  1901 LIFAKEFRGLNGYKPTACVPALELTRKWMQKDPRHVPRR----------------------GERV 1943
            .:.......|||..|   |...|: .|.:.|||:..|.|                      |:.|
Mouse  1119 RLIEIGENVLNGSVP---VSQFEI-NKALTKDPQDYPDRKSLPHVHVALWINSQGGRKVKAGDTV 1179

  Fly  1944 PFIIVNGPPGMQLIRLVRSPHDILANEGHKINAIYYITKAIIPPLNR-C-----------LLLIG 1996
            .::|......:...:...:|..:...:...|:..||:.:.|.|.:.| |           .|.:|
Mouse  1180 SYVICQDGSNLTATQRAYAPEQLQKLDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLG 1244

  Fly  1997 AN-----VHDWFASLPR-KLLMTPAVGTANE----------LAGARGAKSTISQYFSTTSCVIDC 2045
            .:     ||.:...... .||..||..|..|          |..:.|.::.....|..:...::.
Mouse  1245 LDSTQFRVHQYHKDEENDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYDNVFEGSGLDMEP 1309

  Fly  2046 GRQTKAGICPDCLKNATTCVVVLSDK----TARLERGYQLTRQICQ--ACCGRL----------G 2094
            .....:.:  ||..:..|.:|.||:|    ..|..:.|.....||:  .||.||          |
Mouse  1310 SLYRCSNV--DCKVSPLTFMVQLSNKLIMDIRRCIKKYYDGWLICEEPTCCSRLRRLPLHFSRNG 1372

  Fly  2095 SLQCDSLDCPV 2105
            .|      |||
Mouse  1373 PL------CPV 1377

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-zeta	NP_524881.2	PTZ00166	20..>196	CDD:240301
		DNA_polB_zeta_exo	1297..1518	CDD:99821	44/255 (17%)
		POLBc_zeta	1550..2000	CDD:99917	120/531 (23%)
		zf-C4pol	2041..2107	CDD:405022	20/81 (25%)
Pola1	NP_032918.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	20..39		4/26 (15%)
		DNA_pol_alpha_N	41..101	CDD:289058	13/59 (22%)
		pol2	56..1229	CDD:273159	263/1361 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	105..135		5/29 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	261..297		9/49 (18%)
		DNA_polB_alpha_exo	539..771	CDD:99819	44/249 (18%)
		DNA-binding. /evidence=ECO:0000255	654..719		14/67 (21%)
		POLBc_alpha	836..1246	CDD:99915	112/470 (24%)
		DNA-binding. /evidence=ECO:0000255	1249..1380		31/137 (23%)
		zf-DNA_Pol	1269..1458	CDD:286132	26/117 (22%)
		CysB motif	1352..1378		10/32 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810