Protein Alignment comt and afg2b

DIOPT Version :10

• Sequence 1: NP_524877.1
• Gene: comt / 47091
• FlyBaseID: FBgn0000346
• Length: 745
• Species: Drosophila melanogaster
• Sequence 2: NP_001070056.2
• Gene: afg2b / 767648
• ZFINID: ZDB-GENE-060929-204
• Length: 748
• Species: Danio rerio
• Alignment Length: 749
• Identity: 187/749 - (24%)
• Similarity: 292/749 - (38%)
• Gaps: 213/749 - (28%)

  Fly    92 ITCVSFETDFLQ----KKTVS----QEPYDSDQMAKEFIMQFAGMALTVGQSLVFNFKDKKLLGL 148
            :|||..:. |:|    ||.||    ||..|...:.:..::..:|:...:...||
Zfish    95 LTCVKLKV-FVQRLEHKKRVSERSLQELLDGIYVHEGHLVDLSGVKAEIRCVLV----------- 147

  Fly   149 AVKSLEAIDPKSLGEGKDTAMRNVRFGRILGNTVVQFEKAENSSLNLQGKSKGKVVRQSIINPDW 213
                 |.::..|...|..||...|....|  .||...::.:               :|....|
Zfish   148 -----EKVNSGSQRAGLITARTCVDISTI--QTVKHLDRQQ---------------QQVSAAP-- 188

  Fly   214 DFGKMGIGGLDKEFNSIFRRAFASRVFP---PELVEQLGCKHVKGILLYGPPGTGKTLM----AR 271
                  :||::..|.|:....    .||   |..:.|||....:|:||.||||.||||:    |:
Zfish   189 ------LGGMEDVFASLKEMI----TFPLRYPGSLRQLGLSCPRGLLLIGPPGVGKTLLVRCVAK 243

  Fly   272 QIGTMLNAREPKIVNGPQILDKYVGESEANVRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKQ 336
            .||..|     ..||||::.....||||.|:||:|     |:.|...:.|..:::.||||::|.:
Zfish   244 DIGATL-----VTVNGPEVTGSRPGESEENLRRVF-----EQARDAADDGPCVLLIDEIDSLCPR 298

  Fly   337 RGSVAGNSGVHDTVVNQLLTKIDGVDQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQ 401
            |  ...:|...:.:|.||||.:|.:......::||.||:.|.:|.||.||||.:.::.|.:|:..
Zfish   299 R--TGSSSAPENRLVAQLLTLMDAIGSHEGFVIIGATNQPDSLDPALRRPGRFDREVIIGVPSLL 361

  Fly   402 GRVQILNIHTKRMREFNKINDDVDNKEIAALTKNFSGAELEGLVRAAQSSAMNRLIKADAKVTVD 466
            .|..||....:.|    .::.|||...:|.:|..:.||:|..|.|.|...|| |..:..|.   :
Zfish   362 QRRSILKCVCREM----PLSPDVDLNTLAEMTCGYVGADLSALSREAALQAM-RHSQMGAS---E 418

  Fly   467 PEAMEKLKVNRDDFLHSLEH-------------DIKPAFGTAQEILDNMLARGVINW---GAPVS 515
            |.:|:       .|:.:|.|             |.||                 |.|   |.
Zfish   419 PVSMQ-------HFMQALRHVQPSCLRSSIGATDFKP-----------------IGWEQIGG--- 456

  Fly   516 NLLEDGMLYVQQA-----KAPES------SGLVSVLVAGAPNSGKTALAAQLAKMSDFPFVKVCS 569
              |||..|.::|:     :.||:      |....||:.|.|...||.|....|..|...|..:..
Zfish   457 --LEDVKLKLKQSIEWPMRFPEAFVRLGVSRPRGVLLYGPPGCAKTTLVKAAASSSHCSFFSLSG 519

  Fly   570 PEDMVGYT-ESAKCLHIRKIFDDAYRSMLSCIVVDNVERLLDYGSIGPRYS-NMTLQALLVLLKK 632
            .|....|. :|.|.|  .::|..|.....|.:.:|.|:.::.....|...| ::..|.|.|||.:
Zfish   520 AELFSPYVGDSEKTL--AQLFAQARACAPSIVFLDEVDSMVGSREDGSSSSHSVQSQVLSVLLTE 582

  Fly   633 QPPKG----------RKLLILCTSSRREV-LEEMEM----------------------------- 657
            ....|          ||:.:|....:.:| |.:||:
Zfish   583 LDGVGVRTLERRSTCRKIALLEGGDQEDVRLHQMELQEVCNKDVLIVAATNRPEALDSALLRPGR 647

  Fly   658 -----------LTAFTSVLHVPNLSKPDHVLAVLEN----TDIFSKGEIQAIGKKMA-------- 699
                       |.|..:||.:...|.|.|....||:    |::||..:::.:.|:.|
Zfish   648 LDQIIYVPPPDLQARLAVLRICTKSVPLHQDVCLEDLAAQTELFSGADLENLCKEAALLALREDG 712

  Fly   700 ------GKRVFIGIKKLLGLIDMARQTEQSQRAI 727
                  .::.|:   |.|.::..:...||.|:.|
Zfish   713 LAVSCVRQKYFL---KALQMLSPSLSAEQLQQNI 743

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
comt	NP_524877.1	CDC48_N	5..83	CDD:215012
		CDC48_2	111..>158	CDD:215011	5/46 (11%)
		RecA-like_NSF-SEC18_r1-like	219..395	CDD:410912	65/182 (36%)
		CDC48	<220..>606	CDD:273521	124/420 (30%)
		SpoVK	<526..702	CDD:440232	53/257 (21%)
afg2b	NP_001070056.2	CDC48	13..740	CDD:273521	185/744 (25%)