Protein Alignment comt and SPAST

DIOPT Version :10

• Sequence 1: NP_524877.1
• Gene: comt / 47091
• FlyBaseID: FBgn0000346
• Length: 745
• Species: Drosophila melanogaster
• Sequence 2: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Alignment Length: 334
• Identity: 98/334 - (29%)
• Similarity: 150/334 - (44%)
• Gaps: 78/334 - (23%)

  Fly   166 DTAMRNVRFGRILGN-TVVQFEKAENSSLNLQGKSKGKVVRQSIINPDWDFGKMGIGGLDKEFNS 229
            |:.:.|:....|:.| |.|:|:       ::.|:...|...|.|:
Human   321 DSNLANLIMNEIVDNGTAVKFD-------DIAGQDLAKQALQEIV-------------------- 358

  Fly   230 IFRRAFASRVFP---PELVEQLGCKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQIL 291
                     :.|   |||...|... .:|:||:||||.|||::|:.:....||....| :...:.
Human   359 ---------ILPSLRPELFTGLRAP-ARGLLLFGPPGNGKTMLAKAVAAESNATFFNI-SAASLT 412

  Fly   292 DKYVGESEANVRRLFAEAEEEEKRLGPNSGLHIIIFDEIDA-ICKQRGSVAGNSGVHDT---VVN 352
            .|||||.|..||.|||.|.|    |.|:    ||..||:|: :|::|      .|.||.   :..
Human   413 SKYVGEGEKLVRALFAVARE----LQPS----IIFIDEVDSLLCERR------EGEHDASRRLKT 463

  Fly   353 QLLTKIDGVDQL--NNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQIL-NIHTKRM 414
            :.|.:.|||...  :.:||:|.|||...:|||:||  |...::.:|||||:.|:.:| |:..|:
Human   464 EFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLR--RFIKRVYVSLPNEETRLLLLKNLLCKQ- 525

  Fly   415 REFNKINDDVDNKEIAAL---TKNFSGAELEGLVRAAQSSAMNRLIKADAKVTVDPEAMEKLKVN 476
                  ...:..||:|.|   |..:||::|..|.:.|....:..|.....|   :..|.|...:.
Human   526 ------GSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVK---NMSASEMRNIR 581

  Fly   477 RDDFLHSLE 485
            ..||..||:
Human   582 LSDFTESLK 590

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
comt	NP_524877.1	CDC48_N	5..83	CDD:215012
		CDC48_2	111..>158	CDD:215011
		RecA-like_NSF-SEC18_r1-like	219..395	CDD:410912	60/184 (33%)
		CDC48	<220..>606	CDD:273521	87/279 (31%)
		SpoVK	<526..702	CDD:440232
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		98/334 (29%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		2/6 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312
		RecA-like_spastin	343..506	CDD:410932	64/209 (31%)
		AAA_lid_3	533..587	CDD:465537	15/56 (27%)
		Vps4_C	<580..612	CDD:462762	4/11 (36%)