DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment acj6 and POU4F2

DIOPT Version :9

Sequence 1:	NP_524876.1	Gene:	acj6 / 47080	FlyBaseID:	FBgn0000028	Length:	396	Species:	Drosophila melanogaster
Sequence 2:	NP_004566.2	Gene:	POU4F2 / 5458	HGNCID:	9219	Length:	409	Species:	Homo sapiens

Alignment Length:	401	Identity:	195/401 - (48%)
Similarity:	220/401 - (54%)	Gaps:	109/401 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    22 GRYSPSYRS-------SEQMRR-CMPNPSIHISSSCDSLESRLLEDASLLCNSWSARQNGDIFAG 78
            ||.|.|..|       ||.||| |:|.|.                              .:||.|
Human    68 GRSSSSSSSGSSGGGGSEAMRRACLPTPP------------------------------SNIFGG 102

  Fly    79 INDGILSRAEALAAVDIQKHQAQHVHSQMPSQIKHDVMYHHHSMSGPPQRPLQENPFSRQ-MHHS 142
            :::.:|:||||||||||......|               |||    ||    ..:||... .:|:
Human   103 LDESLLARAEALAAVDIVSQSKSH---------------HHH----PP----HHSPFKPDATYHT 144

  Fly   143 MDQLDMLDPTGSMTTLAPISE-SPLTPTHQHLHGSYHSMNHMMSHHH---------------PGT 191
            |:.:.......|.:  .|||. |.|..||.|.|..:|       |||               ||.
Human   145 MNTIPCTSAASSSS--VPISHPSALAGTHHHHHHHHH-------HHHQPHQALEGELLEHLSPGL 200

  Fly   192 LSGHTGG----------HHGHSAVHHPVITAAVAAAGLH---------PDTDTDPRELEAFAERF 237
            ..|...|          |..|.|..:|:..||::.|..|         .|.|.|||:||||||||
Human   201 ALGAMAGPDGAVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERF 265

  Fly   238 KQRRIKLGVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQA 302
            |||||||||||||||.||||||:||||:||||||||||||||||||||||||||||||||||
Human   266 KQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAE--- 327

  Fly   303 KNKRRDPDAPSVLPAGEKKRKRTSIAAPEKRSLEAYFAVQPRPSGEKIAAIAEKLDLKKNVVRVW 367
            |:.|.....|.:....||||||||||||||||||||||:|||||.||||||||||||||||||||
Human   328 KSHREKLTKPELFNGAEKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVW 392

  Fly   368 FCNQRQKQKRI 378
            ||||||||||:
Human   393 FCNQRQKQKRM 403

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
acj6	NP_524876.1	POU	222..299	CDD:197673	70/76 (92%)
acj6	NP_524876.1	Homeobox	323..377	CDD:395001	51/53 (96%)
POU4F2	NP_004566.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	26..93		11/24 (46%)
		Required for transcriptional activation. /evidence=ECO:0000250\|UniProtKB:Q63934	91..237		51/207 (25%)
		POU-IV box	110..119		8/8 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	153..188		14/43 (33%)
		Nuclear speckle targeting signal. /evidence=ECO:0000269\|PubMed:19266028	171..185		7/20 (35%)
		Required for DNA-binding and transcriptional repression. /evidence=ECO:0000250\|UniProtKB:Q63934	238..409		133/169 (79%)
		POU	250..327	CDD:197673	70/76 (92%)
		Homeobox	348..401	CDD:278475	50/52 (96%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165156605
Domainoid	1	1.000	141	1.000	Domainoid score	I4710
eggNOG	1	0.900	-	-		E1_KOG3802
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	299	1.000	Inparanoid score	I2711
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D396215at33208
OrthoFinder	1	1.000	-	-		FOG0001337
OrthoInspector	1	1.000	-	-		otm51489
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR11636
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2889
SonicParanoid	1	1.000	-	-		X830
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.890