Protein Alignment MYH4 and ck

DIOPT Version :10

• Sequence 1: NP_060003.2
• Gene: MYH4 / 4622
• HGNCID: 7574
• Length: 1939
• Species: Homo sapiens
• Sequence 2: NP_523571.1
• Gene: ck / 34882
• FlyBaseID: FBgn0000317
• Length: 2167
• Species: Drosophila melanogaster
• Alignment Length: 2244
• Identity: 553/2244 - (24%)
• Similarity: 898/2244 - (40%)
• Gaps: 574/2244 - (25%)

Human    27 AQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATV-TVKEDQVFSMNPPKYDKIED 90
            |..:.||..            :.|.|.|.||.::..:.:.|..| ...|.::.:|:......:||
  Fly    15 ASGREFDVA------------IGARVVSAEGRRIQVRDDDGDEVWLAPERRIKAMHASSVQGVED 67

Human    91 MAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHI 155
            |..:..|||..:|.||..||...:||||:|...|.||||:.||:|..:.:..|:.:|..|.||||
  Fly    68 MISLGDLHEAGILRNLLIRYKENLIYTYTGSILVAVNPYQILPIYTGDQIKLYKERKIGELPPHI 132

Human   156 FSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLE 220
            |:|.||||..|...|::|.|:|:||||||||.:||.::||.|.|            ||| ...:|
  Fly   133 FAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAI------------SGK-HSWIE 184

Human   221 DQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERS 285
            .||:.|||:|||||||||:||||||||||:|.|||.|.|.:..|.||.|||||||:..|..:||:
  Fly   185 QQILEANPILEAFGNAKTIRNDNSSRFGKYIDIHFSANGVIEGAKIEQYLLEKSRIVSQNHSERN 249

Human   286 YHIFYQIL----SNKKPEL-IEMLLITTNPYDFAFVSQG-EITVPSIDDQEELMATDSAVDILGF 344
            ||:||.||    :::|..| :.|..      |:.:::.| .||....||..|.....||:.:|.|
  Fly   250 YHVFYCILAGLSADEKSRLDLGMAA------DYKYLTGGNSITCEGRDDAAEFSDIRSAMKVLLF 308

Human   345 TADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEV-----ADKAAYLTSLNSADLLKSLCY 404
            :..|...|.||..|::|.||:|:|....:..   |.||:     .::.|.|..|....|:.:|..
  Fly   309 SDQEIWEIIKLLAALLHCGNIKYKATVVDNL---DATEIPEHINVERVAGLLGLPIQPLIDALTR 370

Human   405 PRVKVGNEFVTKGQTVQQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPR---QYFIGVLDIA 466
            ..:....|.|....:..|..:...|..|.||.:||:.:|.:||..:  .:||   :..||||||.
  Fly   371 RTLFAHGETVVSTLSRDQSVDVRDAFVKGIYGRMFVHIVRKINTAI--FKPRGTSRNAIGVLDIF 433

Human   467 GFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIE-KP 530
            |||.||.||.||.|||:.||.|||||..|:|.||||||..|.|.|:.|:| :|....::||. |.
  Fly   434 GFENFDQNSFEQFCINYANENLQQFFVQHIFKLEQEEYNHEAINWQHIEF-VDNQDALDLIAIKQ 497

Human   531 MGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAG 595
            :.|.::::||..|||.||.:...||::.| |...|:.||   |......|.|.|:||.|.|:..|
  Fly   498 LNIMALIDEEARFPKGTDQTMLAKLHKTH-GSHKNYLKP---KSDINTSFGLNHFAGVVFYDTRG 558

Human   596 WLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLN 660
            :||||:|..:..::.|..:|..|.|..:|  ||..|.        |.:......|:|..||::|:
  Fly   559 FLDKNRDTFSPDLLHLVSQSTNKFLRQIF--AQDIEM--------GAETRKRTPTLSTQFRKSLD 613

Human   661 KLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQR 725
            .||..|.|..|.|:|||.|||.|.|...:..|...|||.:|::|.|||.|.|:|.|..:.:|.:|
  Fly   614 ALMKTLSSCQPFFIRCIKPNELKKPMMFDRGLCCRQLRYSGMMETIRIRRAGYPIRHGFREFVER 678

Human   726 YKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLIT 790
            |:.| ...:|.....|.:.|:.::. ::.:..:.|:.||||||.|......||:.||..|.:.|.
  Fly   679 YRFL-IPGVPPAHRTDCQAATSRIC-AVVLGKSDYQLGHTKVFLKDAHDLFLEQERDRVLTRKIL 741

Human   791 RTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKL-YFKIKPLLKSAETEKEMA 854
            ..|...||::.|   |:.:..|.:...:|...:.:...|.:..|:: |.:::.|::|..
  Fly   742 ILQRSIRGWVYR---RRFLRLRAAAITVQRFWKGYAQRKRYRNMRVGYMRLQALIRSRV------ 797

Human   855 NMKEEFEKTKEELAKTEAK------RKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCDQLI 913
             :...|...:..:...:|.      |:|...||..:::.::.::..:         |..|
  Fly   798 -LSHRFRHLRGHIVGLQAHARGYLVRREYGHKMWAVIKIQSHVRRMI---------AMRR----- 847

Human   914 KTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENK 978
            ..|::||.|     :.||         ..:.||||::  ||..            ...|||.|..
  Fly   848 YRKLRLEHK-----QFAE---------VLQLRKLEEQ--ELLH------------RGNKHAREIA 884

Human   979 VKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDD---LEG 1040
            .::..:.:..|         |::.:||    .|::.:..|..:|.:..|..|.|:.|||   :|.
  Fly   885 EQHYRDRLHEL---------ERREIQE----QLENRRRVEVNMNIINDAARKQEEPVDDGKLVEA 936

Human  1041 SLE---------------QEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFE 1090
            ..:               :|..:..||..|:...:.|:    .:.:....|::.|:| .|.::|.
  Fly   937 MFDFLPDSSSDAPTPHGGRETSVFNDLPHAQNVNQDDI----IAPIHISEDEEDLSE-FKFQKFA 996

Human  1091 MSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLE 1155
            .:..||.:..:.|     :|.:|.....:....:::.|: |......:...|:........:|::
  Fly   997 ATYFQGNVNHQYA-----KKALKHPLLPLHTQGDQLAAQ-ALWITILRFTGDMPEPKYHTMDRMD 1055

Human  1156 EAG--GATSAQIEMNKKREAEFQK---MRRD----LEES--TLQHEATAAALRKKHADSVAELGE 1209
            ...  ...:|.:..|..|..|||:   |..|    |::.  :::|:..:..|::|:     :|||
  Fly  1056 TTSVMSKVTATLGRNFIRSKEFQEAQLMGLDPDAFLKQKPRSIRHKLVSLTLKRKN-----KLGE 1115

Human  1210 QI-----------DSLQ-----RVKQKLEK---------EKSELKMEI-----NDLASNMETVSK 1244
            .:           ||.|     |....|||         .::||:.||     ..|.:|....|.
  Fly  1116 DVRRRLQDDEYTADSYQSWLQSRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLTNNPLKSSH 1180

Human  1245 AK------------ANFEKMCRTLEDQLSE-----IKTKEEEQQRLIN-----------ELSAQK 1281
            |:            |..||....|...:.|     ....||..:|..|           ||.|.|
  Fly  1181 ARGWILLSLCVGCFAPSEKFVNYLRAFIREGPPGYAPYCEERLKRTFNNGTRNQPPSWLELQATK 1245

Human  1282 AR--------------------LHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEE 1326
            ::                    ..|.:.|...||.:|.::..|.     .|:..|     .|.::
  Fly  1246 SKKPIMLPITFMDGNTKTLLADSATTARELCNQLSDKISLKDQF-----GFSLYI-----ALFDK 1300

Human  1327 TKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEE-- 1389
            ..:..:....:..|...|    |||.:||        |..:.|   |.||..:..:......|
  Fly  1301 VSSLGSGGDHVMDAISQC----EQYAKEQ--------GAQERN---APWRLFFRKEIFAPWHEPT 1350

Human  1390 ---------LEEAKK--KLAQRLQDAEEHVEAV--------NSKCASLEKTKQRLQNEVEDLMID 1435
                     .::..:  |..:...|.||.:..:        .|...|:|:....|.|.:.|..:.
  Fly  1351 HDQVATNLIYQQVVRGVKFGEYRCDKEEDLAMIAAQQYFIEYSTDMSMERLFTLLPNFIPDFCLS 1415

Human  1436 -----VERSNAACIALDKKQ-----------------------------------RNF------- 1453
                 :||..|..:...||.                                   ||.
  Fly  1416 GVDKAIERWAALVLQAYKKSYYVKDKIAPLKIKEDIVSYAKYKWPLLFSRFYEAYRNSGPNLPKN 1480

Human  1454 DKVLA-EWKQKY---EETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQ---- 1510
            |.::| .|...|   ::.|..||.|..|..::|::  |....:.::.. |.|::.|....|
  Fly  1481 DVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQ--KTNKVFTQTFS-LSTVRGEEFTFQSPNA 1542

Human  1511 QEISDLTEQIAEG----GKHIHELEKVKKQLD--------------------------------- 1538
            ::|.||.....:|    .|::..|:..:...|
  Fly  1543 EDIRDLVVYFLDGLKKRSKYVIALQDYRAPSDGTSFLSFFKGDLIILEDESCGESVLNNGWCIGR 1607

Human  1539 ----HEKSELQT--------------------SLEEA-------------------------EAS 1554
                .|:.:...                    ::|||                         |.:
  Fly  1608 CDRSQERGDFPAETVYVLPTLSKPPQDILALFNIEEAHHGRRLSMASNGGAVEPRDRPHTLMEYA 1672

Human  1555 LEHEEGKILRIQLELNQVKSEIDRKIAEKDEEL-----DQLKRNHLRVVESMQSTLD-------A 1607
            |:|       .:|...:..|:.....:::.|||     |.:|...||.::|.:...:       |
  Fly  1673 LDH-------FRLPPKRTMSKTLTLSSKRSEELWRYSRDPIKAPLLRKLQSKEEFAEEACFAFAA 1730

Human  1608 EIRSRNDALRIKKKMEGDLNE--------MEIQLNHANRQAAEALRNLRN--------------- 1649
            .::...|....:.:|..::.:        .||..:....|..:.|.:.||
  Fly  1731 ILKYMGDLPSKRPRMGNEITDHIFDGPLKHEILRDEIYCQLMKQLTDNRNRMSEERGWELMWLAT 1795

Human  1650 -----TQGILKDTQLHLDDAIR--GQDD---LKEQLAMVERRANLMQAEVEELR----------- 1693
                 :||:||:..|.|.....  .||.   |::.:...:|:....|.|||.::
  Fly  1796 GLFACSQGLLKELLLFLRTRRHPISQDSMHRLQKTIRHGQRKYPPHQVEVEAIQHKTTQIFHKVY 1860

Human  1694 --------ASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGE------ 1744
                    ..::.:.|.:....    :.|:|:.|..::..||.  .|..:..||..:|:
  Fly  1861 FPDDTDEAFEVDSSTRAKDFCN----NISQRLSLRTSEGFSLF--VKIADKVISVPEGDFFFDFV 1919

Human  1745 --MEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTV------KDLQLRL 1801
              :.|.:::||...:.|....|.....                |||....||      .||....
  Fly  1920 RHLTDWIKKARPIRDGANPQFTYQVFF----------------MKKLWTNTVPGKDRNADLIFHY 1968

Human  1802 DEAEQLALKGGKKQIQKLEARVRELESEVE-SEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNI 1865
            .:.....|:|..|..::..|::..|...|. .|.|..::|:      .:.::||.      ..:|
  Fly  1969 HQELPKLLRGYHKCSREEAAKLAALVFRVRFGENKQELQAI------PQMLRELI------PSDI 2021

Human  1866 LRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAK 1899
            :::|...:..::.|.:|.:......|.:.|...|
  Fly  2022 MKIQSTSEWKRSIVASYNQDGGMTSEDAKVAFLK 2055

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MYH4	NP_060003.2	Myosin_N	33..77	CDD:460670	9/44 (20%)
		MYSc_Myh4	100..770	CDD:276879	275/684 (40%)
		Actin-binding. /evidence=ECO:0000250	659..681		11/21 (52%)
		Actin-binding. /evidence=ECO:0000250	761..775		7/13 (54%)
		Myosin_tail_1	850..1927	CDD:460256	241/1404 (17%)
ck	NP_523571.1	MYSc_Myo7	77..721	CDD:276832	275/684 (40%)
		MyTH4	<1138..1245	CDD:214535	24/106 (23%)
		FERM1_F1_Myosin-VII	1249..1347	CDD:340612	21/122 (17%)
		B41	1251..1467	CDD:214604	37/240 (15%)
		FERM_C1_MyoVII	1461..1559	CDD:270019	21/100 (21%)
		SH3	1560..1624	CDD:473055	4/63 (6%)
		MyTH4	1701..1849	CDD:214535	28/147 (19%)
		FERM2_F1_Myosin-VII	1854..1951	CDD:340613	18/118 (15%)
		B41	1856..2068	CDD:214604	40/234 (17%)
		FERM_C2_MyoVII	2064..2159	CDD:270020