Protein Alignment MYH3 and Myo28B1

DIOPT Version :10

• Sequence 1: NP_002461.2
• Gene: MYH3 / 4621
• HGNCID: 7573
• Length: 1940
• Species: Homo sapiens
• Sequence 2: NP_723294.1
• Gene: Myo28B1 / 53515
• FlyBaseID: FBgn0040299
• Length: 2122
• Species: Drosophila melanogaster
• Alignment Length: 2127
• Identity: 543/2127 - (25%)
• Similarity: 855/2127 - (40%)
• Gaps: 542/2127 - (25%)

Human    18 RKSEKERIEAQNQ------PFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLV----- 71
            ||.|...::.||.      ||.|:                         .|.||..:|||
  Fly     8 RKGEYVWVKPQNTTSEFAVPFGAR-------------------------IVRTEKTQTLVCDDRN 47

Human    72 ----VKPEDVY-AMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKW 131
                |...||. ||:....:.:|||..|..|.|..:|.||::||...:||||:|...|.:|||:.
  Fly    48 KQFWVPAGDVLKAMHITSQEDVEDMITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPYQI 112

Human   132 LPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYF 196
            ||:|....::.||.|...|.|||||:|||||:|.:...:|||.::|:||||||||.:||.::||.
  Fly   113 LPIYTNREIQLYRNKSLAELPPHIFAISDNAFQRLQRLKENQCVVISGESGAGKTESTKLILQYL 177

Human   197 ATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLAS 261
            |.|           |.....:|.|||.|||::|||||||||||||||||||:|.|.|...|.:..
  Fly   178 AAI-----------SGKHSWIEQQIIEANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQGAIQG 231

Human   262 ADIETYLLEKSRVTFQLKAERSYHIFYQILSN-KKPELIELLLITTNPYDYPFISQGE-ILVASI 324
            |.|:.|||||||:.||.:.||:|||||.:|:. ...|...|.|...:|..|.:::||. ..:...
  Fly   232 ARIQQYLLEKSRIVFQSRDERNYHIFYCMLAGLSTAERERLKLQEQSPSQYHYLAQGGCFTLPGR 296

Human   325 DDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQ--REEQAEPDGTEVADKTAY 387
            .||::.....:|:.:|.|.|||...:..|..|::|.||::|...:  ....||.|.|....:.|.
  Fly   297 GDAKDFADIRAAMKVLSFKPEEVWSILSLLAAILHLGNLRFTATEVANLATAEIDDTPNLQRVAQ 361

Human   388 LMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKL 452
            |:|:..|.|..||....:.|..|:||...:.:......:|..||:|:.:|:.:|.|||:.::.::
  Fly   362 LLGIPISALNAALTQRTIFVHGEHVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVRRINETINKQV 426

Human   453 PR-QHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGM 516
            .: .:.||||||.|||.|:.||.||||||:.||.|||||..|:|.:||:||:.|.|.|..|:| .
  Fly   427 DQPMNSIGVLDIFGFENFDNNSFEQLCINYANENLQQFFVGHIFKMEQDEYQNEHINWQHIEF-Q 490

Human   517 DLAACIELI-EKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSL 580
            |....::|| .|||.:.|:::||..|||.||.:...||:.|| |..:.:.|.|..:   .:.|.:
  Fly   491 DNQQILDLIGMKPMNLMSLIDEESKFPKGTDQTLLEKLHVQH-GNRSIYVKGKTTQ---TSLFGI 551

Human   581 IHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSF 645
            .||||.|.|:..|:||||:|..:..:..|.|:|:|:.|..::......|       .|||:
  Fly   552 RHYAGVVMYNPLGFLEKNRDSFSGDLRTLVQRSTNKYLVDIFPHEMPMD-------TAKKQ---- 605

Human   646 QTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGF 710
            .|:...||.:|:.||..|...||:|:|||.|||.|.|...:..|.:.|||.:|::|..||.|.|:
  Fly   606 PTLCVKFRNSLDMLMRTLSQAHPYFIRCIKPNEYKEPKNFDKELCVRQLRYSGMMETARIRRAGY 670

Human   711 PNRILYGDFKQRYRVLNASAIPEGQFIDSKK----ACEKLLASIDIDHTQYKFGHTKVFFKAGLL 771
            |.|..|..|.:|||:|.....|..| .|.:|    .||..|.: |.|.   ::|.||:|.:....
  Fly   671 PIRHAYRAFVERYRLLVPPVGPLEQ-CDCRKLARQICEVALPA-DSDR---QYGKTKLFLRDEDD 730

Human   772 GTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQR-RESIFCIQYNIRSFMNVKHWPWMKLFF 835
            .:||..|...:.|.|...|   || :.||.|::.::| ||:|..:|         ::|
  Fly   731 ASLELQRSQLMLKSIVTIQ---RG-IRRVLFRRYMKRYREAIITVQ---------RYW------- 775

Human   836 KIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLL 900
                  :.....::...|::.|.:....:|..:...|      .|:|:.:.   :::||.|..
  Fly   776 ------RGRLQRRKYQVMRQGFHRLGACIAAQQLTTK------FTMVRCRT---IKLQALSRG-- 823

Human   901 DAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKV 965
                   .|::..||     |::.||.:.                   ::|||:      .|.|:
  Fly   824 -------YLVRKDFQ-----KKLLERRKQ-------------------NQLKKE------ELLKL 851

Human   966 EKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSL------NKT 1024
            .|.|.|         |||..|.:...:..||::..||...|....|:||....|:|      .|.
  Fly   852 AKMKEA---------EELLRLQQLKEQKEREQREQQEKRLQEEQRLKAEAAARNALAMAAVQQKR 907

Human  1025 KSKLEQQVEDLESSLEQEKKL---------RVDLERNKRKLEGDLKLAQES--ILDLENDKQQLD 1078
            ::|..:|......:|:....|         ...|..........:....||  .:|:|:.||.:|
  Fly   908 RTKPVKQEAPKAPTLQARNSLPPPPTTLIVAAPLPTRPASAVTRINTIPESPGTIDVESSKQMVD 972

Human  1079 ERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARE 1143
            :     .|.:      :.||....|   :|:..:.:     .:.|...::.....:.....|
  Fly   973 D-----VFRF------LNDEPDAAL---RKLNNISS-----GDTIRLPKSVPNNIDTSDFSY--- 1015

Human  1144 LEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKK------------ 1196
               |.......||..:.|.|            |:.|:::.|:||..:..:..|
  Fly  1016 ---LKYAATYFGGGATAQHE------------RKPLKKSLLKHEHPIDEMASKAIWLTILRFMGD 1065

Human  1197 --------------HADSVAELGEQIDNLQRVKQKL----------------EKEKSEFKLEIDD 1231
                          :.:.:::|...::.....|.:|                |||..||.....:
  Fly  1066 LPDVVSSPTLHVFDNENLMSDLASLLNTSDSYKPRLFVRQSQRRIPKPLASGEKEAQEFYQHWLN 1130

Human  1232 LSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQL---- 1292
            :.:|         :||||...:...:.:...::|.:.:...:|....||.....|.|...|
  Fly  1131 VPTS---------HLEKIHFIIGHGIIKNSLRDEILAQICKQLYLNPSRSSYSRGWLLLSLCLSC 1186

Human  1293 ----EEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQE 1353
                :|.|..:....:...|..|.|..|:|
  Fly  1187 FPPSKEFEPHLRSFMKQGTAQLQATPSLQR----------------------------------- 1216

Human  1354 GKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEE---QV-------EAV 1408
                |:|.|  .|....|..:.:|..||:....|     :|...|.|.::   ||       |||
  Fly  1217 ----LERTL--VNGPRCQPPSLFELHAIRGRHPL-----RLDIHLMDGQQRRLQVDAASTAREAV 1270

Human  1409 NAKCASLEKTK-------QRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWK-------- 1458
            |..|..:..|.       ..|.|::..|....|..  |.|..:.:||..|   |.||
  Fly  1271 NQLCQGMGLTDTFGFGLVMSLNGKLMPLGAGQEHV--LDAISECEQRQLD---APWKLYIRKEMF 1330

Human  1459 -----------------------TKCEESQAELEASLKESRSLST-------ELFKLKNAYEEAL 1493
                                   .||.|.:...|..:....:|:.       |:.:||.:...|.
  Fly  1331 ATWYDPSMDPKATQLIYKQILNGLKCGEYRCRSEKDIAMVCALACFVEYGPGEILRLKPSEITAF 1395

Human  1494 ---DQLETVKRENKNLEQEIADLTEQIA----ENGKTIHELEKSRKQ------------------ 1533
               |.|...:|..:|..:.||...|:.:    |....:.|.:|..|:
  Fly  1396 VPSDLLAPGERAIENWSRLIAATYEKSSYVKEEQNDLLLEAQKRAKEDICLFAHLSWPMRHSRLF 1460

Human  1534 -------IELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNY 1591
                   .:|:..::.|.:..|...|..|..::|     .:...||:.:...|.|:::..:...:
  Fly  1461 EVVRKEGPKLQSDELMLGINSAGLFLIDETEQVL-----ASCCFSEVLKVHVESDDKLHVMTFQH 1520

Human  1592 QRTVETMQSALDA---------EVRSRNE-AIRLKKKMEGDLN-----------EIEIQLSHANR 1635
            ...|....||.||         .:|.|:. .:.|...:||||.           |.|..::.|..
  Fly  1521 VNFVLQCSSAQDANEVINYMLDNLRQRSSYGVALDPVVEGDLEDCLVLNPGDLIEFEAGVTGAQL 1585

Human  1636 QAAETLKHLRS-VQGQ--------------LKDTQLHLDDALRG---QEDLK------------- 1669
            .|.......|. |.||              |......|.|.||.   ||..|
  Fly  1586 MAGNAQDCYRGCVNGQWGQFLAGNVRVLATLTKPSEKLQDILREGRFQEPPKPTPRANYSRRRQH 1650

Human  1670 --EQLA-------IVERRANLLQAEVEELRA-TLEQTERARKLAEQELLDSNERVQLLH------ 1718
              .|||       :...:|.|.:...|.|:| .|:...:...|.:|.|:..:..::.:.
  Fly  1651 NISQLAESHFREPLDSDKAPLSKFSPEPLKAPLLKAVVKVPPLFQQALVMHHHILKYMGDIARSN 1715

Human  1719 -TQNTSLIHTKKKLETDLM------QLQSEVEDASRDARNAEEK-------AKKAITDAAMMAEE 1769
             ..||.||. :..|:..|:      ||..::.|  ..:..:|::       |...:..:.::..|
  Fly  1716 LPVNTDLIF-QPALQHPLLCDELYCQLMKQLSD--NPSSESEKRGWDLLYLATGLVAPSVLVMRE 1777

Human  1770 L------KKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELE 1828
            |      :.:....|.|:|:|::|.|                  |.:|:...|        .|:|
  Fly  1778 LIILLRMRADALADACLKRLKRSLAQ------------------GQRKKAPHL--------IEVE 1816

Human  1829 GEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVK 1875
            |.|::.......:...:..|:  .::.|...:....:.|:..:|::|
  Fly  1817 GIQQRCLHIYHKIYFPDDTVE--AFEIESHTRGAELIADIAQRLELK 1861

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MYH3	NP_002461.2	Myosin_N	33..77	CDD:460670	8/52 (15%)
		MYSc_Myh3	100..767	CDD:276878	273/676 (40%)
		Actin-binding	656..678		11/21 (52%)
		Actin-binding	758..772		4/13 (31%)
		Myosin_tail_1	847..1924	CDD:460256	228/1271 (18%)
Myo28B1	NP_723294.1	MYSc_Myo7	81..726	CDD:276832	273/676 (40%)
		IQ	765..785	CDD:197470	5/41 (12%)
		Atrophin-1	<820..>946	CDD:460830	35/173 (20%)
		MyTH4	1031..1239	CDD:470587	41/269 (15%)
		FERM1_F1_Myosin-VII	1243..1334	CDD:340612	24/100 (24%)
		B41	1248..1429	CDD:214604	38/185 (21%)
		PH-like	1454..1547	CDD:473070	16/97 (16%)
		MyTH4	1674..1819	CDD:214535	32/173 (18%)
		FERM2_F1_Myosin-VII	1825..1919	CDD:340613	5/39 (13%)
		B41	1827..2032	CDD:214604	5/37 (14%)
		FERM_C2_MyoVII	2028..2121	CDD:270020