Protein Alignment MYH3 and Myo81F

DIOPT Version :10

• Sequence 1: NP_002461.2
• Gene: MYH3 / 4621
• HGNCID: 7573
• Length: 1940
• Species: Homo sapiens
• Sequence 2: NP_001303492.1
• Gene: Myo81F / 26067052
• FlyBaseID: FBgn0267431
• Length: 2082
• Species: Drosophila melanogaster
• Alignment Length: 2165
• Identity: 500/2165 - (23%)
• Similarity: 844/2165 - (38%)
• Gaps: 535/2165 - (24%)

Human    90 DMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPH 154
            ||..::.::|..:..||..||.|.:||||:|...:.|||||.:.::|.:.:..|...|..:..||
  Fly    12 DMTEMSEIDEIGINQNLNVRYNSDIIYTYTGSILLAVNPYKTINIFNKQTISEYHTCKLGDLSPH 76

Human   155 IFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLED 219
            :|::::.||..::.|..||:.:|:||||||||.:||.::||..||.          |.:...::.
  Fly    77 VFAVAEAAYTSIVDDNINQACVISGESGAGKTESTKFILQYLCTIT----------SNVSSWVQQ 131

Human   220 QIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSY 284
            ||:.||.:|||||||||.|||||||||||:::.|.....:....|..||||:||:|||.:.||:|
  Fly   132 QILEANTILEAFGNAKTTRNDNSSRFGKFMQVCFDDNNCIKGCVILDYLLEQSRITFQSEGERNY 196

Human   285 HIFYQILSNKKPELIELLLITTNP---YDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEE 346
            ||.||:::..:..:.........|   |.|...|..|.:..:: ::::..|...|..:|......
  Fly   197 HIMYQLVAQGQKNIGIANTFHLRPAEFYKYLNTSNSEKINMNL-ESKKFDAVTMAFTVLQIPQFV 260

Human   347 KSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKT----------AYLMGLNSSDLLKALC 401
            ..|::|:..:::..||::|        .:.|| ||.|.|          :.|:||:..|..|.|.
  Fly   261 IDGVFKVISSILWLGNIEF--------IDVDG-EVTDLTRSDQEAISIISMLLGLSRCDFKKVLL 316

Human   402 FPRVKV-GN--EYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQ-----QLDTKLPRQHFI 458
            ..::.| ||  |...|.:...:..|   ||:|::|.|:|.|:|::||.     |.|.|     |:
  Fly   317 IRQINVRGNITEIPLKIKEALENRH---ALAKALYSKMFTWLVSKINNCTNPGQDDAK-----FL 373

Human   459 GVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIE 523
            |||||.|||.|.:||.||||||:|||||.:||||::|.|||..|:.|||.:..::| .|...|:|
  Fly   374 GVLDIFGFENFSHNSFEQLCINYTNEKLHKFFNHYVFALEQSIYEHEGIYFNHVEF-TDNTPCLE 437

Human   524 LIEK-PMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQK-PKVVKGRAEAH----FSLIH 582
            ::|| |..:|.:|.|:|..|:.:|.:|.:.::.:       |:. |..:||....|    |.:.|
  Fly   438 ILEKPPRCVFKLLTEQCHMPQGSDLAFLSNMHTE-------FESHPNYIKGSDRRHWETEFGIKH 495

Human   583 YAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQT 647
            |||.|.|:|.|::|||:| :.:.|:..|...|..:.....:|:  .|.........:....|..|
  Fly   496 YAGLVIYAVGGFVEKNRD-VQQDVMFEYMSYSKDVFIKDLSTY--QDIQFINSSYPRGSSKSKCT 557

Human   648 VSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPN 712
            ||..||..|..|:..|:.|.|.:||||.||..|.|.....||||.||:..||::.|||.|:|||.
  Fly   558 VSDNFRNQLQSLIDVLQNTKPWYVRCIKPNLQKRPNNYNSSLVLDQLKYLGVVDIIRIRREGFPI 622

Human   713 RILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEM 777
            .:.:.||..||:.|    |....|...:|....:|....:..|:::.|.:|:|.:......||..
  Fly   623 HLSHKDFIVRYKCL----IKNKSFEYPQKYLTNILEKNGMPTTEWQIGKSKIFLRNAAYELLETN 683

Human   778 RDDRLAK-----------------------LITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNI 819
            |.|.|.|                       .:.|.|...||:.:|:.|.:|   |.|...||..:
  Fly   684 RKDTLYKNAVIIQKNWKKYYIQKSFLRNKQAVLRIQHAYRGWRLRIRFMRM---RRSAIVIQSRL 745

Human   820 RSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQE 884
            |...                       .:|:|....|.::..:||    .||..:.|:|.::.
  Fly   746 RGVF-----------------------AREVAAALREMKRVDEEL----KKRDGIYEQLTSIT-- 781

Human   885 KNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECS 949
                       ::.|.|.|....:.:.....:...|:...:...|:.:.::. ||:        |
  Fly   782 -----------TKTLADCERSIQEELSVLAHISDNIRASAKPLSDKNDSDSN-TAR--------S 826

Human   950 ELKKDIDDLELTLAKVEKEKHATENK------------VKNLTEELSGL---------DETIAKL 993
            .:|::..:|:.....:.:.|.|.||.            ||||.||: ||         .:...||
  Fly   827 SIKQNTVNLDNMFDFLCESKGAFENALIKEINDNWNNLVKNLDEEI-GLCIHTEDSFDSQKTGKL 890

Human   994 --TREKKALQE-------AHQQALDDLQAEE--DKVN--SLNKTK--SKLEQQVEDLESSLEQEK 1043
              |:..:.|.|       .......:|.:|.  :.:|  .|..||  .|.:..:.|.:|..:..|
  Fly   891 FTTKTVRTLPEPTVPPPPPPVSISANLNSEPIYEAINLYKLTTTKEIKKEKYMLLDYKSIKDNSK 955

Human  1044 ---------KLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDF--EYCQLQSKVED 1097
                     |.::|...|:||...:.|:.:.:::|:..|....:......:|  .|........|
  Fly   956 KPSTSSVFVKYQIDERENRRKQRVEKKIHELTLIDIHKDIHIDNSFYNILEFAENYFNTHDLSSD 1020

Human  1098 EQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELE------------ELSER 1150
            ...:....||.        :..::..:.|....:|::|..:.:....:            ||.:.
  Fly  1021 CNFISTPTQKG--------KSSDKTAKHEMTMFSKSDKIPTSHIHMYDPENVVLSCSMFRELCKY 1077

Human  1151 LEEAGGVTSTQIELNKKREAEFLK--LRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQR 1213
            :..         |||.:||.:.::  :...:|...|:.|..:..:|:...:      ..||...|
  Fly  1078 MRG---------ELNAERELQIIQYIVGLGIEREELRDEIFIQCIRQSRNN------PNIDWTDR 1127

Human  1214 VKQKL-------EKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSL 1271
            :...|       :..|..|:..:..:..||..:.   ..|.:..:...|.....:........|.
  Fly  1128 IWIILCLSIVAFQPSKLLFRYFVSFIKKSMIELD---GKLRQYSQWCFDNCKSTKVSTRMYPPSS 1189

Human  1272 SELTTQKSRLQT---------------------EAGELSRQLEEKESIVS--------------- 1300
            .|:...: ||.|                     .||:..::|.||.::.:
  Fly  1190 VEVAAMR-RLGTIVCRFFFLDARTKAIDVHPTDTAGDAVQKLAEKLNLTTTDGWAIYQNRPDGEE 1253

Human  1301 ---------------QLSRSKQAFTQQTEELKRQLEEENKAKNAL---AHALQSSRHDCDLLREQ 1347
                           ::.::..|.|::|.......|.:...|..|   .|.|.....:..:|..|
  Fly  1254 HIKSYDYLYDVISSWEMKQATYAGTKKTTNGSSSGENQFVFKKRLFKSTHELSQDPVEVSMLYSQ 1318

Human  1348 Y--------EEEQEGKAELQRALSKANSEVA------QWRTKYETD-------AIQRTEELEEAK 1391
            .        |.....|..||  |:...::||      |.:.:|.:|       .|.:|.|.:...
  Fly  1319 AVHSVVKKDEFPVSEKVALQ--LAGLQAQVALGDPSNQPKPEYYSDINSFLPIRISKTREQQFWI 1381

Human  1392 KKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAAL------------- 1443
            ..|||..:.......|:.||...|....|..........|:.:...:||:.:
  Fly  1382 PILAQAHRQYGSSRNALTAKVLYLSCVMQYPLYGTTMFKVNYKGYWNLASNIILGIHYDGIMFIN 1446

Human  1444 --DK------KQRNFDKVLAEWKTKCEESQAELEASLKES-RSLSTELFKLKNAYEEALDQLETV 1499
              ||      |..:.:.:|      .:.|.:.:..||..| :.|.|.|:.......:.....||.
  Fly  1447 PEDKSVVFQFKYVDIESIL------LDPSDSFITISLNRSAQLLKTSLYGSSLIESQKCFVFETP 1505

Human  1500 KRENKNLEQEIADL--TEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRI 1562
            ::      .:|..|  :...|.:...::.||.|:|.....|.| :|.|        ::...:.|.
  Fly  1506 QK------NDIGSLIISYYPALSNWILNNLECSKKYKGTTKED-RLRL--------YQNVVLCRR 1555

Human  1563 QLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIE 1627
            .|        :|..|.:|..:.....||..|                    ||.|..   |.::.
  Fly  1556 HL--------VDMNIVKKPHDFGGFLRNTLR--------------------RLSKHR---LEKLR 1589

Human  1628 IQLSHANRQAAETLK---H-------------LRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVE 1676
            .::....::..||.|   |             :.::..|.:...:.:.|::.....|......:.
  Fly  1590 SEVGSNTQEHGETYKGFSHTFWAFSKQQIPFCISTISDQEEALMIQVFDSILTYSGLGSSGESIS 1654

Human  1677 RRANLLQAEVEELRATLEQTERARKLAEQELL---------------DSNERVQLLHTQNTSLIH 1726
            |      ||.|.:|......::..|  ::.||               |:|.||.|   :|.:|:.
  Fly  1655 R------AEDEHIRIIQSIMDKCMK--KESLLNELYLQLIKQTTDHPDANSRVNL---KNWALLS 1708

Human  1727 ------------TKKKLETDLMQLQSE-VEDASRDARNAEE------------------------ 1754
                        .:|.|...|.:..|: :.:..:.||.||.
  Fly  1709 VLCSVILPSMKAVRKYLIAHLKRCSSDFLSEEGKYARYAENCFFRTQGTRRRQWTPSREEILCTT 1773

Human  1755 -----KAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALK---GGKK 1811
                 .:|....|....:.|.:.....:..||.:||.:        ..||.|:..::.   |..:
  Fly  1774 NRRPCYSKFYFMDGQYYSIEFQPSSTANDVLEIIKKKI--------GLLDNAKGYSIYEVIGNSE 1830

Human  1812 QIQKLETRIREL-----EFELEGEQ--KKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLV 1869
            :....|.::.::     ::::..:|  :|||..:....:|....|:..:..     |.:.|:|:|
  Fly  1831 RSLSSEEKVCDVMAKWEKYQITSQQGFQKNTTLISQQNQYMFLFKKHLFFD-----NYINLEDIV 1890

Human  1870 DKLQVKVKSYKRQAEEADEQANAH--LTKFRKAQHELEEAE-----------ERADIAESQVNKL 1921
            :|                 :...|  |...|..::.:.|.|           ||.|..|| :...
  Fly  1891 EK-----------------ELLYHQILHNLRSERYPITEMEAIMLTALQSQLERGDCCES-ITDY 1937

Human  1922 RAKTRDFTSSRMV---VHES 1938
            ||........|.|   .||:
  Fly  1938 RAVASHCLPPRFVPNIPHEA 1957

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MYH3	NP_002461.2	Myosin_N	33..77	CDD:460670
		MYSc_Myh3	100..767	CDD:276878	247/693 (36%)
		Actin-binding	656..678		10/21 (48%)
		Actin-binding	758..772		3/13 (23%)
		Myosin_tail_1	847..1924	CDD:460256	227/1339 (17%)
Myo81F	NP_001303492.1	Motor_domain	23..673	CDD:473979	247/692 (36%)
		MyTH4	1091..1194	CDD:459939	16/111 (14%)
		FERM_F1_DdMyo7_like	1198..1299	CDD:340728	12/100 (12%)
		B41	1202..1419	CDD:214604	36/218 (17%)
		PH-like	1415..1523	CDD:473070	19/119 (16%)
		MyTH4	1664..1771	CDD:459939	18/111 (16%)
		FERM_F1_DdMyo7_like	1775..1880	CDD:340728	18/112 (16%)
		B41	1781..1992	CDD:214604	39/208 (19%)
		PH-like	1988..2076	CDD:473070