Protein Alignment Nup214 and npp-14

DIOPT Version :9

• Sequence 1: NP_652039.2
• Gene: Nup214 / 46091
• FlyBaseID: FBgn0010660
• Length: 1711
• Species: Drosophila melanogaster
• Sequence 2: NP_492568.1
• Gene: npp-14 / 172813
• WormBaseID: WBGene00003800
• Length: 1390
• Species: Caenorhabditis elegans
• Alignment Length: 1794
• Identity: 400/1794 - (22%)
• Similarity: 641/1794 - (35%)
• Gaps: 571/1794 - (31%)

  Fly     5 APDCEDTQDFQFKLHHKIVAF--KKCGEPRSNVNL---LAVSSSRGLLFAGSPTQPELKVIIVKD 64
            |.|.....||.|....|...|  |..|..::.:|:   :|.||..|:.|. :....:|.....|.
 Worm     7 AEDVSQVTDFHFHTCRKFRLFSSKSDGYSQNEINIRNRVATSSQLGVTFV-TVNSNQLSCFHTKS 70

  Fly    65 LVNAKSTAQQPQARLVPLP---------SIPNYIACSSDGNLLAVNHTQNGTSLLSIYAVLSFMT 120
            |:..|.|.:.....:..||         .:.|.:..:|||.:|.|.||:|....:.::.:....|
 Worm    71 LLGYKITRENMNVEVTDLPIKTIRLHGVVLINDMGVNSDGTVLGVLHTKNNDVSVDVFDIKKICT 135

  Fly   121 PD----VRPVYNIRLAAEDHVHGVQLLWNPVLPNSLAVVLSNGAL--AMYALKEGGNFEMHSLDK 179
            ..    .:|:...|:..|....|..|.|||..|::.|...::.::  |...::...|.::..:.|
 Worm   136 SSSIEPFKPLCTTRVGTEQINQGSCLEWNPAFPDTFAASSTDRSILVAKINVQSPANQKLVGIGK 200

  Fly   180 NQQVKCG-CWSPKGKQIVLGFPGGTVKQFKPDLTLAKTLLCPPNIHDAPFDTIAIQWLSTFQFAV 243
            ...|... .|||||||:.:|...|.:.|.||:|.:.::...|.|..:....| .:.||:|.:: :
 Worm   201 FGAVTTAISWSPKGKQLTIGDSLGKIVQLKPELEVVRSQHGPENKPNYGRIT-GLCWLATTEW-L 263

  Fly   244 IFLQHGEDCSPSLYILNAPKAGAPSYINYYDICYSMNG--------PRNHQFVFSHVPQWNLLLV 300
            :.|::|.|  ...|::...|.....:|.::::.||.:.        |.....|     .||:::|
 Worm   264 VSLENGTD--QDAYLMRCKKDKPTEWIQFHELSYSSSKWPLPPQLFPATQLLV-----DWNVVIV 321

  Fly   301 VSANGVEVGIMRSTEAGDTPAWQQLTLLDEARIEMPLSEDKDETFPLGFAFDTSTTHQLTIN--- 362
            .::...|:     :..|....||....::...|.:|.:....:|.|:|.|.|.|.|.::.:|
 Worm   322 GNSKTSEI-----STVGKRDDWQTWVPVEGESIYLPTTSSGKDTVPIGVAVDRSMTDEVLLNPDG 381

  Fly   363 EKKLQTMPMVHVLSSDGELLSFNFLNVLPTAVSVCSPPPPVADTSGQFKPLNMLLASEEEEQPAW 427
            .::.:..|:|..|::||.|.:.:.::.....:.        ...|.|...:|.|...:.:.|...
 Worm   382 SQRHRPSPLVLCLTNDGILTAHHIISTFAAHIP--------CQMSSQNLAINDLKKLQFDSQKPI 438

  Fly   428 AASPSKAPAATPAASSDISFAFTPNTVTSTPAPSKDKQPSLFSGFGAAAAKAPAPQLSFGTAPTS 492
            :|.||..               ||.|..||....|.:..:|.|..                  ..
 Worm   439 SAPPSDQ---------------TPVTKPSTVFGQKPEAETLKSSL------------------VG 470

  Fly   493 SPVSFGAPTTNAAKPTTPFGGFGTQATTTAMGSMFSASGANAFGGMALNKPAIASVTPRTAAPGS 557
            ||.|...|     ||::               |:|             |..:||| ...|:....
 Worm   471 SPSSVQTP-----KPSS---------------SLF-------------NPKSIAS-NIETSQLTE 501

  Fly   558 TVPATPASAPANKPLYTVPLTFTPVDTKPATSAPPQIADESLKPDDTEPII--KDMIALQIEAFS 620
            :.|:||| ||:::|         .:.:.|.:.|.|:|:|::|:....|.|.  |.::..:::..:
 Worm   502 SKPSTPA-APSSQP---------KIASTPKSEAIPKISDKTLEHKKAELIATKKQVLIERMDKIN 556

  Fly   621 KDIQKQKEQTKELLKGI------------AAPSALRAYAKRLDDLQEL-----------NEQAKD 662
            ..:...|:.|.:|...:            ...::|....:.:|:|:.|           ....|:
 Worm   557 DSMAGAKDATMKLSFAVGKVKTTIMECADVVRASLGDSKEVMDELKNLILSIERMSDRTQHTVKE 621

  Fly   663 VEFELDVQ-GLRQGLNEAYAIVAECRG-----KLEIYRKPE----------------ITRLMNSS 705
            ::||:|.: .|..|:.:...::.:.|.     ||..:.|.|                |.:|..|.
 Worm   622 MDFEIDEKMELVAGVEDGNQVLEKLRNMSETEKLMRFNKLETAADLLNGKYEECSDLIKKLRMSL 686

  Fly   706 SCDPSGR--------RMLARLQSYVAANEAQLRLAQ---------------QHVDLQWEQFQ-DV 746
            |...|.|        |:.:.|....:.:|.:|.|..               |..:|::.:|| ||
 Worm   687 SEKESLRKQAILSPLRLSSNLNQLRSGSETELALKVMRNVSKIIMDTREQIQRTELEFVRFQRDV 751

  Fly   747 VRRNSK---------SRMHMPCLEGIY---QRLTRLQNLTSNQRIVQNNIKSK--LKERGLLQAA 797
            ..:|.|         ..:.|..|:|..   :.||..:::...|.:| |.|:.:  :|.|.::..:
 Worm   752 KFQNFKKGKENLNFTQPLEMSSLDGDAPQGKSLTDAESIKVRQALV-NQIQKRGIVKTRNVIVES 815

  Fly   798 LLDQEKSRTRTNEAVDT--LTDSILTMSLSQVVDSNAAKLTRERLQKIRNIVQLQKINVICPQRP 860
            ....|.|....|:.:||  |:::||.:|::                                  |
 Worm   816 YKKSENSAAMKNDLLDTSNLSNAILKLSMT----------------------------------P 846

  Fly   861 DRVGLKSEVILETKRRAEQIKRAAAKPATANKYTQAAVAPPSPPDVAPTPAVAPMPQATVTVAPP 925
            .||...|.:.             :|.|:|.:..:.||.....||.|         ....|||..|
 Worm   847 RRVMPSSSLF-------------SASPSTPSTKSDAATQADEPPIV---------KTVVVTVESP 889

  Fly   926 LPKPMPSIPSVVEKPGVPTHPTTPVATPFSFSQSIPFVKTSTVTPTTNTVTP-----------GE 979
             .||:.|.|:|                      |.|.:|.:|.|.||...||           .:
 Worm   890 -AKPIASAPAV----------------------SSPLIKLNTTTATTTMTTPKVTVPKEEANKTQ 931

  Fly   980 AAKPGLSIFGGSTI-SSFSFGGGAAKSAL------SFGTGSPAVAAPTPKPNPLSAVEKPT---- 1033
            ..||.:|....|:| ||.|..|...::.|      :..||.|::...:...:| ..:||.:
 Worm   932 DQKPIISTPASSSIFSSGSLFGTKTQTPLVSKEESTLTTGVPSLINSSLSISP-QEIEKASSKVE 995

  Fly  1034 ----PEPTKPKEQKAAESKEFKAVQPETEESKVPQKPK--AETENKSFGFGGFTGTGGTVGNTSS 1092
                .|..|.::.:...:.:.|:.:|::.|:||.::||  .:|..|.      ..||..:..|.|
 Worm   996 TLNKTEEVKDEKSENEVTPDLKSEEPKSLETKVKEEPKPAVQTPVKE------EETGSNIQKTPS 1054

  Fly  1093 SPFSFGGLGSSLGFGGTAAAVPKSEPSSTATTSVATSASTAPFGIFSAALAKPSNSEPITTVTSN 1157
              |||..               .:.|.||::||          .||...|             ..
 Worm  1055 --FSFNS---------------TTTPKSTSSTS----------SIFGGGL-------------KT 1079

  Fly  1158 TTTITSKPTNVIASSSVTDAPSVTTTNAVTSSTDPIGGLFSSVTICKPNTPADTTKPANIFGGGL 1222
            .|..:|..||:..:.        |||.|                     ||...:..::|||||.
 Worm  1080 QTPSSSNSTNIFGAR--------TTTTA---------------------TPTPASNTSSIFGGGS 1115

  Fly  1223 SAGSFSFGGTIDASKGLFGST--KPVATAPTSVTEANNKTDPISTTPSAISTTTATTTVSSPAVV 1285
            .|.|..||.        ||..  :|..|:.
 Worm  1116 KAASSPFGS--------FGQAGCQPAKTSN----------------------------------- 1137

  Fly  1286 PAAVTAAVPATSSTTVTSSTAVPGSAFSFSNAFSTLSAGGAAAPTTSASAPLAAKSPTATSTGNN 1350
            ||..||:|..:.:|..||::|.|.       .|.:..||.:|.|
 Worm  1138 PATSTASVTFSFNTGATSASAKPA-------GFGSFGAGASAKP--------------------- 1174

  Fly  1351 SSNSVFGGGFAVATSTAAPVASPFQSAAKSPVSSANIFGSIPKAETSVFGGATTAPSNTTAAATP 1415
              :|||||      |..||.. |.........|.||..||  ....|..|.|.|  |||:....|
 Worm  1175 --SSVFGG------SVTAPTV-PNVDDGMEDDSMANGGGS--GGFMSGLGNART--SNTSGGNNP 1226

  Fly  1416 DAPPAGLFASAAISNPSPFGSPTTRAPASGGNIFGQAVKPSVFGQPAQAGDSGGSIFGGGSASTP 1480
            .||......||: |:...||        .|||...|..:     :|:.:.::.||  ....||.|
 Worm  1227 FAPKTSTGTSAS-SSSWLFG--------GGGNQQQQQQQ-----KPSFSFNTAGS--SAQQASAP 1275

  Fly  1481 -FGSSSIFGGGNTQGAVGAPAAGSTSIFGQKVFGQSSAAAPAAGGNIFSNPVGSPQASPFGGGGN 1544
             .|:||:||        |||..||...||                           |.|||||.|
 Worm  1276 ATGTSSVFG--------GAPKFGSQPAFG---------------------------AKPFGGGAN 1305

  Fly  1545 SIFGSPATAPPASGGSIFGGGSSSGGFGSFTQTTPAQGGFGGGFGQGGGGSVAQTGFGSPQAPQQ 1609
                    |..:...|||||.:||      |...||.    |||.|...|....:.||....||.
 Worm  1306 --------AGLSKNASIFGGATSS------TTNNPAT----GGFAQFASGQKTSSLFGGGATPQT 1352

  Fly  1610 QTTTPGGFGAKPVFGGSPAFGASPT---FGGGATFGSPK 1645
            .|:         :|||......:||   |||||:..:.|
 Worm  1353 NTS---------IFGGGANTTPAPTSSVFGGGASANANK 1382

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nup214	NP_652039.2	WD40	<31..>233	CDD:225201	53/220 (24%)
		WD40 repeat	38..86	CDD:293791	12/56 (21%)
		WD40 repeat	90..131	CDD:293791	10/44 (23%)
		WD40 repeat	139..173	CDD:293791	9/35 (26%)
npp-14	NP_492568.1	NUP214	<271..400	CDD:293360	30/140 (21%)
		SMC_N	<532..>757	CDD:330553	40/224 (18%)
		Nucleoporin_FG	1109..>1186	CDD:316182	35/155 (23%)
		Nucleoporin_FG	1289..1373	CDD:316182	37/137 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160167252
Domainoid	1	1.000	188	1.000	Domainoid score	I1952
eggNOG	1	0.900	-	-		E1_KOG3630
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	189	1.000	Inparanoid score	I2580
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46620
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0006508
OrthoInspector	1	1.000	-	-		oto19028
orthoMCL	1	0.900	-	-		OOG6_106765
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R851
SonicParanoid	1	1.000	-	-		X5483
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	13	12.780