DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nup214 and AgaP_AGAP008970

DIOPT Version :9

Sequence 1:	NP_652039.2	Gene:	Nup214 / 46091	FlyBaseID:	FBgn0010660	Length:	1711	Species:	Drosophila melanogaster
Sequence 2:	XP_319720.4	Gene:	AgaP_AGAP008970 / 1279934	VectorBaseID:	AGAP008970	Length:	1850	Species:	Anopheles gambiae

Alignment Length:	1963	Identity:	698/1963 - (35%)
Similarity:	951/1963 - (48%)	Gaps:	366/1963 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MAQNAPDCEDTQDFQFKLHHKIVAFKKCGEPRSNVNLLAVSSSRGLLFAGSPTQPELKVIIVKDL 65
            |||.||...|..:.:|||..|:..||..|..::..:|||.:|..||:|||: ...||:|:.::|:
Mosquito     1 MAQPAPLGLDVTELKFKLQSKVPIFKGDGNAKNGCSLLATASVHGLVFAGT-VGAELRVLKLQDI 64

  Fly    66 VNAKSTAQQPQARLVPLPSIPNYIACSSDGNLLAVNHTQNGTSLLSIYAVLSFMTPDVRPVYNIR 130
            ...:...:....|.|||||.|..:|.|.|..:|||:...||...:.||:|.||:|..:..:..|:
Mosquito    65 TADRVINETVPLRTVPLPSEPYQLAVSCDHGMLAVDVVANGVPFVYIYSVPSFLTGSIVKLAEIK 129

  Fly   131 LAAEDHVHGVQLLWNPVLPNSLAVVLSNGALAMYALKEGGNFEMHSLDKN---QQVKCGCWSPKG 192
            .:.:..|...||.||||:.|.|||....|.|::|.|||....|.||||.|   ::.:|.||||||
Mosquito   130 TSPQPAVRSTQLCWNPVMHNVLAVRTEAGGLSVYTLKEPTGLEFHSLDANNPAERAQCACWSPKG 194

  Fly   193 KQIVLGFPGGTVKQFKPDLTLAKTLLCPPNIHD--APFDTIAIQWLSTFQFAVIFLQHGEDCSPS 255
            ||:|:.|..|.:.|:||||..|:|::|||.:.:  ..|:.:|:|||||:|||.:|:.|.:|..|:
Mosquito   195 KQLVVAFANGKLVQYKPDLKPARTIVCPPGVLEGGGGFEVLAVQWLSTYQFAAVFMPHRDDTVPA 259

  Fly   256 LYILNAPKAGAPSYINYYDICYSMNGPRNHQFVFSHVPQWNLLLVVSANGVEVGIMRSTEAGDTP 320
            |:|:||||||:|.:|||.|||||.:|||..|....|:..|||||:.|||.:||||:.:||:|:.|
Mosquito   260 LFIVNAPKAGSPVFINYDDICYSQSGPRRGQVFLQHILPWNLLLMASANSMEVGILGTTESGEAP 324

  Fly   321 AWQQLTLLDEARIEMPLSEDKDETFPLGFAFDTSTTHQLTINEKKLQTMPMVHVLSSDGELLSFN 385
            .|.|.|..||||.|:||:.||.||||:|.|.:|..||:|.|.|:....|||:|:||:.|:|:|||
Mosquito   325 TWCQWTTTDEARAELPLTADKQETFPIGLALETGCTHELVIGEQTFPVMPMIHLLSTYGQLVSFN 389

  Fly   386 FLNVLPTAVSVCSPPPPVADTSGQFKPLNMLLASEEEEQPAWAASPSKAPAATPAASSDISFAFT 450
            .||.:|...::||||.|:.|.:|...|  ....|..:..||         ||.|..||:|||| .
Mosquito   390 VLNTMPNVPNICSPPKPIQDLTGGAFP--KAFPSMNQNVPA---------AAAPPPSSEISFA-V 442

  Fly   451 PNTVTSTPAPSK--------------DKQP-SLFSGFGAAAA-------KAPAPQLSFGTAPTSS 493
            ||..|||||.:|              .|.| :||.|.|..||       :.|.|     .||..:
Mosquito   443 PNGATSTPAIAKSKSFFNAGVGAEAVQKSPINLFGGAGGTAAPGQQQQQQQPKP-----AAPAIA 502

  Fly   494 PVSFGAPTTNAAKP--------------TTPFGGFGTQATTTAMGSMFSASGANAFGGM-ALNKP 543
            | :||...|..|.|              .:||.||.|.|.....|...:..|....||: .::.|
Mosquito   503 P-TFGKQITFGASPGAGMQQQGVGGETKPSPFAGFSTPAGGGGGGGGTTFGGGTTAGGLPTMSAP 566

  Fly   544 ----AIASVTPRTAAPGSTVPATPASAPANKPLYTVPLTFTP-----------VDTKPATSAPPQ 593
                |:|::.|......|:.|    ||.|||||.|||.::.|           |..:|.|:|.|:
Mosquito   567 PFQAAMAAMGPPPQQSASSRP----SADANKPLVTVPPSYVPAVQQQPPNNGNVAARPQTTAAPK 627

  Fly   594 IADESLKPDDTEPIIKDMIALQIEAFSKDIQKQKEQTKELLKGIAAPSALRAYAKRLDDLQELNE 658
            ..|.|  .:|...||:.:...::..|::::.:.:.:.:.|...|..........:.|.:|:::..
Mosquito   628 RVDFS--SEDNNAIIRALTNDELNRFARELTELQRRNRALSVQIGQKEESAQMIRSLRELEDIIA 690

  Fly   659 QAKDVEFEL--DVQGLRQGLNEAYAIVAECRGKLEIYRKPEITRLMNS-SSCDPSGRRMLARLQS 720
            ||.:....|  |||.||.|||||:|:|||...|..||..|.:.|...: .....:.||.|:.|::
Mosquito   691 QANESTQSLVADVQALRLGLNEAFAMVAEANSKSAIYNNPTVHRYQEAHGGLSQTSRRQLSALEN 755

  Fly   721 YVAANEAQLRLAQQHVDLQWEQFQDVVRRNSKSRMHMPCLEGIYQRLTRLQNLTSNQRIVQNNIK 785
            .:..||:|||...:.::.||...::..:..:|.|||:|.||.:||.|::.|::.:.|......:|
Mosquito   756 MLQVNESQLRTVTKQLEAQWMGLEEAKQARAKQRMHIPSLEVLYQTLSKQQDILNRQGEKLAVLK 820

  Fly   786 SKLKERGLLQAALLDQEKS------------RTRTNE-AVDTLTDSILTMSLSQVVDSNAAKLTR 837
            |||..|..:: .|.|:.||            .|.|:| |:::|||||::|:|...|.::..||:.
Mosquito   821 SKLGLRSSVK-GLEDRNKSVKGGKQLLGGGDATDTSESAIESLTDSIISMTLGDQVAADTKKLSE 884

  Fly   838 ERLQKIRNIVQLQKINVICPQRPDRVGLKSEVILETK---RR----AEQIKRA-AAKPATANKYT 894
            .:|..:|..:..:|:..:.|||||||||:|||:.|.:   ||    .||.|:| .|:.|.|:|..
Mosquito   885 GKLSALRRALMGRKVVTVKPQRPDRVGLRSEVVREHRDQVRRQVVMVEQEKQAKVAQKAAASKAE 949

  Fly   895 QAAV---APPSPPDVAPTPAVAPMPQATVTVAPPLPKPMPSIPSVVEKPGVPTHPTTPVATP--- 953
            |:.|   ...:||...|    |..|..:.:..|...|||.:.|:  .|......|::..|.|
Mosquito   950 QSKVQQQRSSAPPQQQP----AAQPVQSQSKQPVGNKPMATAPA--NKAFSFAVPSSGAAAPPSV 1008

  Fly   954 --FSFSQSIPFVKTSTVTPTTNTVTPGEAAKPGLSIFGGSTISSFSFGGGAAKSALSFGTGSPAV 1016
              |||.|       :|:||.  :.....||||             :..||:..|.|||||
Mosquito  1009 GSFSFGQ-------TTITPM--SAAESNAAKP-------------TANGGSITSGLSFGT----- 1046

  Fly  1017 AAPTPKPNPLSAVEKPTPEPT-KPKEQKAAESKEFKAV------QPETEESKVPQKPKAETENKS 1074
              |.   |..|..:|...|.| ......|:.|..|.:|      :|.:.:|..|..||     ..
Mosquito  1047 --PV---NKESGGDKEKKESTISSSSAPASGSFSFGSVASAGTFKPPSSDSSAPAAPK-----PV 1101

  Fly  1075 FGFGGFTGTGGTVGNTS-----SSP---FSF-------GGLGSSLGFGGTAAA----VPKSEPSS 1120
            |.||. ||||...|..|     |.|   |||       |..|.|.|..|:::|    :...:|.:
Mosquito  1102 FSFGN-TGTGSNSGQQSGLTFGSIPKPNFSFDAPSTTAGSGGLSFGLAGSSSASEGPLSLGKPKA 1165

  Fly  1121 TATTSVATSASTAP-------------FGIFSAALAKPSNSEPITTVTS------NTTTITSKPT 1166
            |||.|.|......|             ||....|   |:...|:..:||      ::..::.|..
Mosquito  1166 TATASTANENVPPPVEEVQKDKPLPPSFGPVEPA---PATKAPLPALTSLLQKVDSSVDLSVKGA 1227

  Fly  1167 NVIASSSVTDAPSVTTTNAV-------TSSTDPIGGLFSSVTICKPNTPADTT-----------K 1213
            :..|::..|..||......:       |||:...||||.|||  ||..|:..|           |
Mosquito  1228 SGGAATGATSQPSKPAGGGLFGSITGGTSSSFATGGLFGSVT--KPTAPSAATNNASANEEGGAK 1290

  Fly  1214 PANIFGG---GLSAGSFSF-----GGTIDASKG--LFGSTKPVATAPT-SVTEANNKTDPISTTP 1267
            ||...||   |||.||.|.     ||  ||:.|  ||||.||...|.| .:|.....:...|...
Mosquito  1291 PAASGGGLFSGLSFGSLSVTPSSAGG--DAASGGSLFGSVKPANGAATGGLTFGGGSSFASSGFG 1353

  Fly  1268 SAISTTTATTTVSSPAVVPA-------------AVTA------AVPATSSTTVTSSTAVPGSAFS 1313
            .|.|:|..|.||:|||..||             |.|.      |.|:|:||.|.||   ||..||
Mosquito  1354 VAKSSTDTTATVASPATEPAKTAGKEESTATATATTGFGFGSIAAPSTTSTAVVSS---PGGGFS 1415

  Fly  1314 FSNAFSTLSAGGAAAPTTSASA--------PLAAKSPTATSTGNNS------------------- 1351
            |:...:|.||.....|||:|:|        .::..||||.|.|||:
Mosquito  1416 FAPVAATSSATTTTTPTTTAAADTTSTLFGSISICSPTAASAGNNAKTTPSITGGNIFSSGTFGA 1480

  Fly  1352 ------SNSVFGGGFAVATSTAA-PVASPFQSAAKSP----VSSANIFGSI--PKA--------- 1394
                  :.|:||||...||:||| ..||||...|.||    .||..:|||:  |.|
Mosquito  1481 SKPTTDTKSIFGGGGGGATATAAGGAASPFGPLAASPAPAAASSGGLFGSVGQPTAASTALGAAS 1545

  Fly  1395 ---ETSVFGGATTAPSNTTAAATPDAPPAGLFASAAISNPSPFGSPTTRAPASGGNIFG---QAV 1453
               .||||||.....|.|.||.|                     :||:..|:||| .||   .|.
Mosquito  1546 TTTTTSVFGGGFFGASTTAAAGT---------------------APTSTTPSSGG-FFGGGAGAA 1588

  Fly  1454 KPSVFGQPAQAGDSGGSIFGGGSASTPFGSSSIFGG-GNTQGAVGAPAAGSTSIFGQKVFGQSSA 1517
            ..||||  :.|..:..:|||..:::|..|.:.:||. ..:..|...||.|  ||||....|.:||
Mosquito  1589 TNSVFG--SSAASTTSNIFGSAASATSTGGTGLFGSVAASNSATPGPAGG--SIFGGGGGGGASA 1649

  Fly  1518 --AAPAAGGNIFSNPVGSPQASPFGGGGNSIFGSPATAPPASGGSIFGGGSSSGG--FGSFTQTT 1578
              .|..:|||||.:||.:..|||.|.|.   ||||.|.|.:|..||||||::|||  |||...|.
Mosquito  1650 FGGAGGSGGNIFGSPVAAAAASPAGAGP---FGSPQTPPQSSTQSIFGGGAASGGGAFGSSVATG 1711

  Fly  1579 PA--QGGF-GGGFGQGGGGSVAQTGFGSPQAPQQQTTTPGGFGAKPVFGGSPAFGASPTFGGGAT 1640
            .|  .|.| .|..|.|.....:.||..:...|.....|| .||..|.|||:|.||.:|||||..|
Mosquito  1712 AAASPGPFSSGAAGTGVNAFASPTGTSAFSKPPAFGATP-TFGGAPTFGGAPTFGGAPTFGGAPT 1775

  Fly  1641 FGSPKGFGGFGGASPVASPPPFGAAAKPAQG-------NIFETLGGQESGLSFGNLAQTGNSNAQ 1698
            |||||  ..|||...|....|||..|.||.|       |:||.||...||:|||.||  ....|:
Mosquito  1776 FGSPK--ATFGGGGVVGGGSPFGGGASPAIGSPATQSNNLFEQLGSSSSGVSFGGLA--AQQQAK 1836

  Fly  1699 KPAFGGSSFMNYR 1711
            ...||||||.::|
Mosquito  1837 PTQFGGSSFSSWR 1849

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nup214	NP_652039.2	WD40	<31..>233	CDD:225201	81/206 (39%)
		WD40 repeat	38..86	CDD:293791	18/47 (38%)
		WD40 repeat	90..131	CDD:293791	15/40 (38%)
		WD40 repeat	139..173	CDD:293791	16/33 (48%)
AgaP_AGAP008970	XP_319720.4	Phasin	<741..817	CDD:298582	23/75 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	855	1.000	Domainoid score	I229
eggNOG	1	0.900	-	-		E1_KOG3630
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	855	1.000	Inparanoid score	I1273
OMA	1	1.010	-	-		QHG46620
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0006508
OrthoInspector	1	1.000	-	-		oto108129
Panther	1	1.100	-	-	LDO	PTHR23193
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X5483
TreeFam	1	0.960	-	-
	11	10.930