DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NAT1 and EIF4G

DIOPT Version :9

Sequence 1:	NP_001033943.2	Gene:	NAT1 / 46020	FlyBaseID:	FBgn0010488	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_001078319.1	Gene:	EIF4G / 825194	AraportID:	AT3G60240	Length:	1727	Species:	Arabidopsis thaliana

Alignment Length:	1371	Identity:	295/1371 - (21%)
Similarity:	479/1371 - (34%)	Gaps:	451/1371 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   100 GQTPPTPSVG---KTLLNTSPNL-LPITTATDTFNNLKNLVKIDKGSSPSSGSNSLGGNYRKGGS 160
            |||.|...|.   ...:....|| ||   ||:.|.  |.|:|:.. :|.:..|:|:..:..| .:
plant   641 GQTMPDSLVSDPETATVAAKENLSLP---ATNGFR--KQLLKVST-TSDAPTSDSVDTSIDK-ST 698

  Fly   161 NGSGYKSAGGNGGSP----------------------------AISGNSEGGRN---GSR----- 189
            .||.:.|:..:|.||                            .:||..|..:|   |:.
plant   699 EGSSHASSEISGSSPQEKDLKCDNRTASDKLDERSVISDAKHETLSGVLEKAQNEVDGATDVCPV 763

  Fly   190 ---------------------SSGGPTGHFSTGYSKNSVKIRQHDSEVSPRPRRSYQGDSSSSRY 233
                                 ||..|.||..|           |.|.|....||     ::|::.
plant   764 SEKLAVTDDTSSDLPHSTHVLSSTVPLGHSET-----------HKSAVETNTRR-----NTSTKG 812

  Fly   234 YPKNSDIL--GQGLGGNSDSYNGSSSSGRYQQQDQQGGSHRGSRSNYHSNSDGSYNRSINSNSVG 296
            ..|..:||  ....|..||.|  .:..|..::::.....|..|..|.......:....:::..|
plant   813 KKKIKEILQKADAAGTTSDLY--MAYKGPEEKKESSNVVHDVSNQNLLPAIPQAVEAIVDTEPV- 874

  Fly   297 GGGSGAVGSGSGGSNYRSARNYENGNGSNGTQPRYGSGNGNGNSSNSNSSSGYNGSAASST---- 357
                                                          .|....:..:|..||
plant   875 ----------------------------------------------KNEPEDWEDAADVSTPKLE 893

  Fly   358 NSKSSYGASRGGNDG------GVSSGYSSNY-----------RDNYEDSP-------------SH 392
            .:.:|..|.||.:|.      .....||.::           .:.::.||             ||
plant   894 TADNSVNAKRGSSDEVSDNCINTEKKYSRDFLLKFADLCTALPEGFDVSPDIANALIVAYMGASH 958

  Fly   393 QRYNNTNS----LERNGGGGSGAGASGGPRSYGRSDADRYGGTPSSRTPNLRNGSASAQISGSTS 453
            ..:::..:    ::|.   .|||.....|.:.  :..||:       |.|  .||..|...|
plant   959 HEHDSYPTPGKVMDRQ---ASGARLDRRPSNV--AGDDRW-------TKN--QGSLPAGYGG--- 1006

  Fly   454 NSSNISLNSGSASAAG----------------------GGTPHSQQQQERSQAG-----KASTPP 491
               |:....|....:|                      ||...:....||.|.|     |...|.
plant  1007 ---NVGFRPGQGGNSGVLRNPRMQGPIISRPMQPVGPMGGMGRNTPDLERWQRGSNFQQKGLFPS 1068

  Fly   492 PITGRWIPPSLRPQHGL-TQSEKNDAVFRRVRGILNKLTPEKFQELSDELLKLDLNSIAILKGVI 555
            |.|...:......::.: |.:::..|..|:::.|||||||:.|::|.:::..:::::...|.|||
plant  1069 PHTPMQVMHKAERKYQVGTIADEEQAKQRQLKSILNKLTPQNFEKLFEQVKSVNIDNAVTLSGVI 1133

  Fly   556 LLIFDKALDEPKYSSMYAQLCKRLSEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLKRDENDNR 620
            ..||||||.||.:..|||..|..||...|.|: |.....||.|||:..|:::|....|.:|..:|
plant  1134 SQIFDKALMEPTFCEMYADFCFHLSGALPDFN-ENGEKITFKRLLLNKCQEEFERGEKEEEEASR 1197

  Fly   621 PPPE-----NEADEEERRHLAKQRMLGNVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQ 680
            ...|     .|.:.||:|...::|||||::.||||.|..||::.::|.||.:|....:....
plant  1198 VAEEGQVEQTEEEREEKRLQVRRRMLGNIRLIGELYKKRMLTEKIMHACIQKLLGYNQDPHE--- 1259

  Fly   681 EMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWV 745
               |::|.|.:|:.|.|..:|..:.|..|:.||||::..|...|...|:||||.:.|:||:|.|.
plant  1260 ---ENIEALCKLMSTIGVMIDHNKAKFQMDGYFEKMKMLSCKQELSSRVRFMLINAIDLRKNKWQ 1321

  Fly   746 PRKLGTTEGPVPIKQIRSDDDSIIRTPFPNRNRDMRNNDRDSDSWMNRFHLNIQPGGYNDMFSGL 810
            .|.  ..|||..|:::..|.....:|   ..||..|....:|..  .|.|:.         ||
plant  1322 ERM--KVEGPKKIEEVHRDAAQERQT---QANRLSRGPSMNSSG--RRGHME---------FS-- 1368

  Fly   811 SVTGASPIVSPFATSNRDRDRDNRQYNNRGDRNRDRDQGGSGGANYGNRYNKHNQNGGGSGGGSS 875
            |..|...::||.|.                       |.||             .:|...|.|.|
plant  1369 SPRGGGGMLSPPAA-----------------------QMGS-------------YHGPPQGRGFS 1397

  Fly   876 NNRDRDDSRRNNDRDRDRNDGQYGGNQLLGSKELAPRFKRNLITTNQDAV--ENLQMRPAANSLL 938
            |...|.|.|                          |.::..::...|.:|  |.:.:.|...  |
plant  1398 NQDIRFDDR--------------------------PSYEPRMVPMPQRSVCEEPITLGPQGG--L 1434

  Fly   939 FRAASQNQKFPATLPMSTPPNSSGSNNQGSSQGQLS--NSHYPMLGTPTSHLINPTRPSSTPSAE 1001
            .:..|..:...|:....:....:|..:.....|.|:  .||.|  .:|.:|      ..|:|
plant  1435 GQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGSHRP--ASPVTH------GRSSP--- 1488

  Fly  1002 YADNRTLLGVQQERGL-----------KATSSSPNFSSGAEKLDLDGQAGRKGSHDGKDQGKNGS 1055
                       ||||.           :|:..||..||..:.|                ||.:.:
plant  1489 -----------QERGTAYVHREFASLSRASDLSPEVSSARQVL----------------QGPSAT 1526

  Fly  1056 VERPSTPAGGIGSAKQQKKDKGPNKEELSKKASQYFKDRFYYDEEEDAEVTEIEIKDIDETKTEE 1120
            |..|              ::...::|:|...:....|:  ||...::.|: .:.:||::.
plant  1527 VNSP--------------RENALSEEQLENLSLSAIKE--YYSARDENEI-GMCMKDMNS----- 1569

  Fly  1121 ADAAPVEVSEVKEDAAIPVTTPDRFTKLVDGFLELKLPEK-ALKDVCINLLMEVLDRVNDVFLER 1184
                             |...|...:..|....|.|..|: .|..:.:||:....:.:|:|.|
plant  1570 -----------------PAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALNEVQL-- 1615

  Fly  1185 AVRLLQTLRKQSNIKPNIVVEIFKQVVNKMNEREALN--PRIVSLVASLLAKTICEPALLKLSDI 1247
                               |:.|:.|:..:  .:|:|  |:....:..:..|::.| .::.|::|
plant  1616 -------------------VKGFESVLKTL--EDAVNDAPKAAEFLGRIFGKSVTE-KVVTLTEI 1658

  Fly  1248 ANFTDNGQHYPLFLL-----------VLQQLVKVIGKDALEEKFRASKVDLMNSLPEA-DRNKER 1300
            ......|...|..|:           ||:.:....|::.|.|..|:|.:.:.|..|.| :|:|
plant  1659 GRLIQEGGEEPGSLIEFGLGGDVLGSVLEMIKTEAGEETLVEIRRSSGLRIENFKPHAPNRSK-- 1721

  Fly  1301 LAGILE 1306
               |||
plant  1722 ---ILE 1724

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NAT1	NP_001033943.2	MIF4G	518..743	CDD:280935	85/229 (37%)
		RAM	<817..865	CDD:291966	6/47 (13%)
		MA3	1128..1241	CDD:214714	20/115 (17%)
		W2_eIF4G1_like	1314..1460	CDD:211397
EIF4G	NP_001078319.1	MIF4G	1096..1319	CDD:280935	85/229 (37%)
EIF4G	NP_001078319.1	MA3	1546..1654	CDD:295416	26/156 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	154	1.000	Domainoid score	I1351
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D594395at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm2499
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR23253
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	6	6.020